Selective mobilization of CD14+CD16+monocytes by exercise

2000 ◽  
Vol 279 (3) ◽  
pp. C578-C586 ◽  
Author(s):  
Birgit Steppich ◽  
Farshid Dayyani ◽  
Rudolf Gruber ◽  
Reinhard Lorenz ◽  
Matthias Mack ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood ◽  
Adrenergic Receptor ◽  
Initial Level ◽  
Human Peripheral Blood ◽  
Fold Increase ◽  
Anaerobic Exercise ◽  
Late Antigen ◽  
Clinical Conditions ◽  
Dependent Mechanism

Strenuous, anaerobic exercise leads to an increase of leukocytes that are mobilized from the marginal pool. We have analyzed in human peripheral blood the effect of exercise on the number of CD14+CD16+monocytes as determined by two-color immunofluorescence and flow cytometry. We show herein that this type of monocyte responds with a dramatic up to 4.8-fold increase. Mobilization does not occur after 1 min at 100 or 200 W but 1 min at 400 W leads to a twofold increase of the CD14+CD16+monocytes immediately after exercise. The numbers remain high at 5 min and gradually decrease to reach the initial level at 20 min postexercise. After 20 min of rest, the CD14+CD16+monocytes can be mobilized again by a second exercise. The CD14+CD16+monocytes appear to be mobilized from the marginal pool where they preferentially home because of a higher expression of adhesion molecules like CD11d and very late antigen-4. Exercise goes along with an increase of catecholamines, and mobilization of the CD14+CD16+monocytes can be substantially reduced by treatment of donors with the β-adrenergic receptor blocker propranolol. Mobilization of CD14+CD16+monocytes by a catecholamine-dependent mechanism may contribute to the increase of these cells in various clinical conditions.

Abstract 3310: Human Peripheral Blood-derived CD31 + Cells Have Robust Angio-vasculogenic Properties And Enhance Recovery Following Hindlimb Ischemia

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Sung-Whan Kim ◽  
Hyun-Jai Cho ◽  
Ji Yoon Lee ◽  
Yong Jin Choi ◽  
Young-sup Yoon
Keyword(s):  
Peripheral Blood ◽  
Network Formation ◽  
Therapeutic Potential ◽  
Human Peripheral Blood ◽  
Therapeutic Option ◽  
Fold Increase ◽  
Treated Group ◽  
Circulating Endothelial Cells ◽  
Hindlimb Ischemia ◽  
Murine Bone Marrow

Background: Recently, we reported that murine bone marrow derived CD31 + cells have hemangioblastic activities. In this study, we hypothesized that human peripheral blood derived CD31 + cells (hCD31 + ) have robust angio-vasculogenic potential and can induce therapeutic neovascularization in hindlimb ischemia. Methods and Results: hCD31 + cells were isolated using a magnetic bead separation technique. By FACS analysis, we confirmed that more than 98% of hCD31 + cells are positive for CD45, suggesting that hCD31 + cells are not circulating endothelial cells. In endothelial progenitor cell (EPC) culture assay, hCD31 + cells almost exclusively gave rise to EPCs and produced significantly higher colony forming activity than hCD31 - cells (n=5, each) (25.2 ± 2.2 vs. 3.6 ± 0.5/mm 2 , P<0.01). In the matrigel tube formation assay, coculturing HUVEC with hCD31 + cells revealed that hCD31 + cells robustly contributed to network formation with HUVECs suggestive of angio-vasculogenic activity. Real-time PCR showed that hCD31 + cells express higher levels of angiogenic genes (fold increase: angiopoietin-1, 5.6 ± 0.8; HGF, 2.7 ± 0.6; VEGF-A, 3.8 ± 0.5; FGF-2, 2.0 ± 0.5) and lower levels of inflammatory genes (fold decrease: Interferonγ, 13.2 ± 2.5; TNFα, 2.3 ± 0.9) than hCD31 − cells. To investigate therapeutic potential, we surgically induced hindlimb ischemia in athymic nude mice, and injected hCD31 + cells, hCD31 − cells and PBS into the ischemic limbs (n=9, each). The hCD31 + treated group showed a higher limb salvage rate than other groups [total salvage/tip necrosis/amputation; hCD31 + cells 5/4/0, hCD31 − cells 2/5/2, PBS control 0/5/4] (P<0.01). At 14 days, perfusion ratio and capillary density were significantly higher in the hCD31 + treated group compared to the hCD31 − and PBS treated groups. Histologic analysis demonstrated that a fraction of injected DiI-labeled hCD31 + cells exhibit endothelial phenotypes during the follow-up period of 8 weeks. Conclusion: The present study demonstrated that hCD31 + cells have robust angio-vasculogenic potential with low inflammatory activity, and enhanced post-ischemia recovery. These data suggested that hCD31 + cell transplantation may represent a novel therapeutic option for treating ischemic cardiovascular diseases.

scholarly journals Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187440 ◽  
Author(s):  
Bo Langhoff Hønge ◽  
Mikkel Steen Petersen ◽  
Rikke Olesen ◽  
Bjarne Kuno Møller ◽  
Christian Erikstrup
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals OMIP-042: 21-color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood

2017 ◽  
Vol 93 (2) ◽  
pp. 186-189 ◽  
Author(s):  
Karl W. Staser ◽  
William Eades ◽  
Jaebok Choi ◽  
Darja Karpova ◽  
John F. DiPersio
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Color Flow

scholarly journals Incorporation of stilbazolium merocyanines into human leukocytes measured by flow cytometry.

1995 ◽  
Vol 42 (3) ◽  
pp. 333-338 ◽  
Author(s):  
K Wiktorowicz ◽  
M Niedbalska ◽  
A Planner ◽  
D Frackowiak
Keyword(s):  
Flow Cytometry ◽  
Cell Membrane ◽  
Fluorescence Intensity ◽  
Peripheral Blood ◽  
Incubation Temperature ◽  
Human Peripheral Blood ◽  
Dye Molecules ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Human Leukocytes

Human peripheral blood leukocytes were incubated with thirteen various merocyanines of the stilbazolium betaine type and the fluorescence intensities of the cells were measured by flow cytometry. The fluorescence intensity of lymphocytes, monocytes and granulocytes depended on the time and temperature of incubation with the dyes. An increase in the incubation temperature enhanced the fluorescence intensity whereas washing of the cells after incubation had little influence on the observed emission. This points to incorporation of the dye molecules into the cell membrane. From the measured fluorescence intensities corrected for relative fluorescence yields, the relative efficiencies of incorporation into the cells of the various merocyanines tested were evaluated. The efficiency was dependent on the type of the cells and the lenght and side groups of the merocyanine molecules studied.

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