scholarly journals Amniotic fluid-borne hepatocyte growth factor protects rat pups against experimental necrotizing enterocolitis

2014 ◽  
Vol 306 (5) ◽  
pp. G361-G369 ◽  
Author(s):  
Sunil K. Jain ◽  
Eric W. Baggerman ◽  
Krishnan MohanKumar ◽  
Kopperuncholan Namachivayam ◽  
Ramasamy Jagadeeswaran ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Amniotic Fluid ◽  
Necrotizing Enterocolitis ◽  
Antibody Array ◽  
Rat Pups ◽  
Increased Risk ◽  
Fluid Supplementation ◽  
Hepatocyte Growth

Fetal swallowing of amniotic fluid, which contains numerous cytokines and growth factors, plays a key role in gut mucosal development. Preterm birth interrupts this exposure to amniotic fluid-borne growth factors, possibly contributing to the increased risk of necrotizing enterocolitis (NEC) in premature infants. We hypothesized that supplementation of formula feeds with amniotic fluid can provide amniotic fluid-borne growth factors and prevent experimental NEC in rat pups. We compared NEC-like injury in rat pups fed with infant formula vs. formula supplemented either with 30% amniotic fluid or recombinant hepatocyte growth factor (HGF). Cytokines/growth factors in amniotic fluid were measured by immunoassays. Amniotic fluid and HGF effects on enterocyte migration, proliferation, and survival were measured in cultured IEC6 intestinal epithelial cells. Finally, we used an antibody array to investigate receptor tyrosine kinase (RTK) activation and immunoblots to measure phosphoinositide 3-kinase (PI3K) signaling. Amniotic fluid supplementation in oral feeds protected rat pups against NEC-like injury. HGF was the most abundant growth factor in rat amniotic fluid in our panel of analytes. Amniotic fluid increased cell migration, proliferation, and cell survival in vitro. These effects were reproduced by HGF and blocked by anti-HGF antibody or a PI3K inhibitor. HGF transactivated several RTKs in IEC6 cells, indicating that its effects extended to multiple signaling pathways. Finally, similar to amniotic fluid, recombinant HGF also reduced the frequency and severity of NEC-like injury in rat pups. Amniotic fluid supplementation protects rat pups against experimental NEC, which is mediated, at least in part, by HGF.

Effect of hepatocyte growth factor in amniotic fluid on fetal alveolar type II cells

Placenta ◽  
1995 ◽  
Vol 16 (7) ◽  
pp. A4
Author(s):  
Atsuo Itakura ◽  
Osamu Kurauchi ◽  
Tomomitsi Okamoto ◽  
Shigehiko Morikawa ◽  
Kazunori Furugori ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Amniotic Fluid ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Hepatocyte Growth

Levels of hepatocyte growth factor in maternal serum and amniotic fluid

1995 ◽  
Vol 173 (3) ◽  
pp. 937-942 ◽  
Author(s):  
Nobuhito Horibe ◽  
Tomomitsu Okamoto ◽  
Atsuo Itakura ◽  
Toru Nakanishi ◽  
Takanobu Suzuki ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Amniotic Fluid ◽  
Maternal Serum ◽  
Hepatocyte Growth

Function of Hrs in endocytic trafficking and signalling

2001 ◽  
Vol 29 (4) ◽  
pp. 472-475 ◽  
Author(s):  
C. Raiborg ◽  
K. G. Bache ◽  
A. Mehlum ◽  
H. Stenmark
Keyword(s):  
Signal Transduction ◽  
Growth Factors ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Membrane Trafficking ◽  
Structural Similarity ◽  
Kinase Substrate ◽  
Vacuolar Protein ◽  
Hepatocyte Growth ◽  
Tyrosine Kinase Substrate

The hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, becomes tyrosine-phosphorylated upon the binding of various growth factors and cytokines to their receptors. This protein is essential for ventral folding morphogenesis, and it shares structural similarity with Vps27p, which is involved in vacuolar protein sorting in yeast. Since Hrs is localized to endosomes and has been implicated in the regulation of signal transduction as well as membrane trafficking, it has been regarded as a potential co-ordinator of endosomal receptor sorting and signalling. Here we discuss the possible functions of Hrs in light of its interactions with phosphatidylinositol 3-phosphate and multiple proteins.

scholarly journals The Incremental Prognostic Value of Hepatocyte Growth Factor in First-Ever Acute Ischemic Stroke: An Early Link Between Growth Factor and Interleukins

2021 ◽  
Vol 12 ◽  
Author(s):  
Fangfang Li ◽  
Ping Liu ◽  
Yuyou Huang ◽  
Lingzhi Li ◽  
Sijia Zhang ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Acute Ischemic Stroke ◽  
Predictive Accuracy ◽  
Characteristic Curve ◽  
Stroke Outcome ◽  
Net Reclassification Improvement ◽  
Increased Risk ◽  
Hepatocyte Growth

Hepatocyte growth factor (HGF) is a potential prognostic factor for acute ischemic stroke (AIS). In this study, we sought to validate its earlier predictive accuracy within 24 h for first-ever AIS. Moreover, as HGF interacts with interleukins, their associations may lead to novel immunomodulatory therapeutic strategies. Patients with first-ever AIS (n = 202) within 24 h were recruited. Plasma HGF and related interleukin concentrations were measured by multiplex immunoassays. The primary and secondary outcomes were major disability (modified Rankin scale score ≥3) at 3 months after AIS and death, respectively. Elastic net regression was applied to screen variables associated with stroke outcome; binary multivariable logistic analysis was then used to explore the relationship between HGF level and stroke outcome. After multivariate adjustment, upregulated HGF levels were associated with an increased risk of the primary outcome (odds ratio, 7.606; 95% confidence interval, 3.090–18.726; p < 0.001). Adding HGF to conventional risk factors significantly improved the predictive power for unfavorable outcomes (continuous net reclassification improvement 37.13%, p < 0.001; integrated discrimination improvement 8.71%, p < 0.001). The area under the receiver operating characteristic curve value of the traditional model was 0.8896 and reached 0.9210 when HGF was introduced into the model. An elevated HGF level may also be a risk factor for mortality within 3 months poststroke. The HGF level was also positively correlated with IL-10 and IL-16 levels, and HGF before interaction with all interleukins was markedly negatively correlated with the lymphocyte/neutrophil ratio. HGF within 24 h may have prognostic potential for AIS. Our findings reinforce the link between HGF and interleukins.

scholarly journals Autocrine Interactions of Keratinocyte Growth Factor, Hepatocyte Growth Factor, and Kit-Ligand in the Regulation of Normal Ovarian Surface Epithelial Cells*

Endocrinology ◽  
2000 ◽  
Vol 141 (7) ◽  
pp. 2532-2539 ◽  
Author(s):  
Jeff A. Parrott ◽  
Rachel Mosher ◽  
Grace Kim ◽  
Michael K. Skinner
Keyword(s):  
Ovarian Cancer ◽  
Growth Factors ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Ovarian Surface Epithelium ◽  
Surface Epithelium ◽  
Kit Ligand ◽  
Ovarian Surface ◽  
Hepatocyte Growth

Ovarian tumors are primarily derived from the layer of epithelium surrounding the ovary termed the ovarian surface epithelium (OSE). Although extensive research has focused on established ovarian tumors, relatively little is known about the normal biology of the OSE that gives rise to ovarian cancer. The local expression and actions of growth factors are likely involved in both normal and tumorigenic OSE biology. The current study investigates the expression and action of keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), and kit-ligand (KL) in normal ovarian surface epithelium (OSE). The actions of various growth factors on KGF, HGF, and KL expression are examined. Observations indicate that freshly isolated normal OSE express the genes for KGF, HGF, and KL and expression is maintained in vitro. KGF messenger RNA expression in OSE was found to be stimulated by KGF and HGF, but not KL. HGF expression in OSE was found to be stimulated by KGF, HGF, and KL. KL expression in OSE was also found to be stimulated by KGF, HGF, and KL. Therefore, the various growth factors can regulate the mRNA expression of each other in OSE. Effects of growth factors on OSE growth were examined. KGF, HGF, and KL stimulated OSE growth to similar levels as the positive control epidermal growth factor. Observations suggest that KGF, HGF, and KL interact to promote OSE growth and growth factor expression. The ability of these growth factors to interact in a positive autocrine feedback loop is postulated to be important for normal OSE biology. Paracrine interactions with the adjacent stromal cells will also be a factor in OSE biology. Abnormal interactions of these growth factors may be involved in the onset and progression of ovarian cancer.

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