Identification and localization of extracellular Ca2+-sensing receptor in rat intestine

1998 ◽  
Vol 274 (1) ◽  
pp. G122-G130 ◽  
Author(s):  
Naibedya Chattopadhyay ◽  
Ivan Cheng ◽  
Kimberly Rogers ◽  
Daniela Riccardi ◽  
Amy Hall ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Large Intestine ◽  
Rat Kidney ◽  
Cell Types ◽  
Northern Analysis ◽  
Reaction Products ◽  
Polymerase Chain ◽  
Small Intestinal ◽  
Small And Large Intestine

The extracellular calcium ([Formula: see text])-sensing receptor (CaR) plays vital roles in [Formula: see text] homeostasis, but no data are available on its expression in small and large intestine. Polymerase chain reaction products amplified from reverse-transcribed duodenal RNA using CaR-specific primers showed >99% homology with the rat kidney CaR. Northern analysis with a CaR-specific cRNA probe demonstrated 4.1- and 7.5-kb transcripts in all intestinal segments. Immunohistochemistry with CaR-specific antisera showed clear basal staining of epithelial cells of small intestinal villi and crypts and modest apical staining of the former, whereas there was both basal and apical staining of colonic crypt epithelial cells. In situ hybridization and immunohistochemistry also demonstrated CaR expression in Auerbach’s myenteric plexus of small and large intestines and in the submucosa in the region of Meissner’s plexus. Our results reveal CaR expression in several cell types of small and large intestine, in which it may modulate absorptive and/or secretomotor functions.

Tissue-specific expression of mRNA encoding rat kidney water channel CHIP28k by in situ hybridization

1993 ◽  
Vol 264 (1) ◽  
pp. C237-C245 ◽  
Author(s):  
H. Hasegawa ◽  
R. Zhang ◽  
A. Dohrman ◽  
A. S. Verkman
Keyword(s):  
In Situ Hybridization ◽  
Epithelial Cells ◽  
Proximal Tubule ◽  
Tissue Distribution ◽  
Renal Cortex ◽  
Rat Kidney ◽  
Water Channel ◽  
Northern Analysis ◽  
Selective Hybridization

The tissue distribution of mRNA encoding rat kidney water channel CHIP28k was determined by in situ hybridization. cDNA encoding rat kidney CHIP28k was isolated by homology to human erythrocyte CHIP28 (G. M. Preston and P. Agre. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991) and used to construct 155-base 35S-labeled cRNA sense and antisense probes corresponding to base pair 7-162. Fixed and frozen tissues were cut in 6- to 12- microns sections, hybridized with probes at 55 degrees C for 16 h, and exposed for 5-9 days. In renal cortex, CHIP28k mRNA was detected intensely on proximal tubule epithelial cells but not in glomeruli or collecting duct. Hybridization to proximal tubule was strongest in deep renal cortex. In no study was there significant hybridization of sense cRNA probe. In renal papilla, CHIP28k mRNA was detected in only a fraction of tubules corresponding to thin limbs of Henle. Hybridization in spleen was observed in red splenic pulp containing erythroid precursors but not in white pulp. In colon, there was selective hybridization in crypt epithelial cells but not in villus epithelial cells or nonepithelial structures. In lung, hybridization was observed in alveolar epithelial cells. In eye, there was selective hybridization in corneal endothelium and ciliary body. No hybridization was observed in any cell types in liver. Northern analysis revealed a 2.8-kilobase mRNA encoding CHIP28k in kidney cortex and papilla but not in brain, skeletal muscle, and liver. These results indicate a wide and highly selective tissue distribution of mRNA encoding the CHIP28k water channel.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Expression of IL-10 receptors on epithelial cells from the murine small and large intestine

2000 ◽  
Vol 12 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Timothy L. Denning ◽  
Nicola A. Campbell ◽  
Fei Song ◽  
Roberto P. Garofalo ◽  
Gary R. Klimpel ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Large Intestine ◽  
Small And Large Intestine

Description of the gastrointestinal tract and associated organs of the kultarr (Antechinomys laniger)

2013 ◽  
Vol 35 (1) ◽  
pp. 39 ◽  
Author(s):  
Hayley J. Stannard ◽  
Julie M. Old
Keyword(s):  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Large Intestine ◽  
Cell Types ◽  
Post Mortem ◽  
Microscopic Description ◽  
Future Studies ◽  
New Information ◽  
Small And Large Intestine

This paper provides a macro- and microscopic description of the digestive tract of the kultarr (Antechinomys laniger), a small dasyurid marsupial. The digestive tract was simple, with no external differentiation between the small and large intestine, and lacked a caecum. Mean gross length of the kultarr digestive tract was 165.2 ± 32.1 mm. Microscopically, the tissues had cell types similar to those of other mammals. The new information will aid future post-mortem investigations of captive kultarrs and future studies of nutrition.

Region-specific ontogeny of α-2,6-sialyltransferase during normal and cortisone-induced maturation in mouse intestine

2002 ◽  
Vol 282 (3) ◽  
pp. G480-G490 ◽  
Author(s):  
Dingwei Dai ◽  
N. Nanda Nanthakumar ◽  
Tor C. Savidge ◽  
David S. Newburg ◽  
W. Allan Walker
Keyword(s):  
Large Intestine ◽  
Regional Differences ◽  
Trophic Factors ◽  
Mrna Levels ◽  
Age Dependent ◽  
Regional Specificity ◽  
Microvillus Membrane ◽  
Mouse Intestine ◽  
Small Intestinal ◽  
Small And Large Intestine

Regional differences in the ontogeny of mouse intestinal α-2,6-sialyltransferase activities (α-2,6-ST) and the influence of cortisone acetate (CA) on this expression were determined. High ST activity and α-2,6-ST mRNA levels were detected in immature small and large intestine, with activity increasing distally from the duodenum. As the mice matured, ST activity (predominantly α-2,6-ST) in the small intestine decreased rapidly to adult levels by the fourth postnatal week. CA precociously accelerated this region-specific ontogenic decline. A similar decline of ST mRNA levels reflected ST activity in the small, but not the large, intestine. Small intestinal sialyl α-2,6-linked glycoconjugates displayed similar developmental and CA induced-precocious declines when probed using Sambucus nigraagglutinin (SNA) lectin. SNA labeling demonstrated age-dependent diminished sialyl α2,6 glycoconjugate expression in goblet cells in the small (but not large) intestine, but no such regional specificity was apparent in microvillus membrane. This suggests differential regulation of sialyl α-2,6 glycoconjugates in absorptive vs. globlet cells. These age-dependent and region-specific differences in sialyl α-2,6 glycoconjugates may be mediated in part by altered α-2,6-ST gene expression regulated by trophic factors such as glucocorticoids.

Renal expression of the gene for atrial natriuretic factor

1992 ◽  
Vol 263 (5) ◽  
pp. F974-F978
Author(s):  
J. E. Greenwald ◽  
D. Ritter ◽  
E. Tetens ◽  
P. S. Rotwein
Keyword(s):  
Epithelial Cells ◽  
Atrial Natriuretic Factor ◽  
Rat Kidney ◽  
Pcr Amplification ◽  
Northern Analysis ◽  
Mammalian Kidney ◽  
Natriuretic Factor ◽  
Normal Rat ◽  
Gene Transcripts ◽  
Atrial Natriuretic

To date, atrial natriuretic factor (ANF) mRNA has eluded detection in the mammalian kidney, although we and others have identified ANF protein in the kidney using immunohistochemical and immunoassay techniques. Furthermore, we have demonstrated the synthesis and secretion of the ANF prohormone in the distal cortical nephron of the intact rat kidney and from rat primary cultured renal distal cortical tubular epithelial cells. In the present study, we show that the ANF gene is expressed in the kidney. Amplification of RNA isolated from rat distal cortical tubular epithelial cultures using ANF specific primers produced a 213-bp fragment that specifically hybridized to a 32P-labeled ANF cDNA. We had previously demonstrated these cultures to be enriched for the renal ANF synthetic and secretory cell type. However, we were unable to detect an ANF gene transcript in total rat kidney RNA using the above-mentioned polymerase chain reaction (PCR) conditions. Reanalysis of normal rat kidney PCR products by a second round of PCR amplification using nested primers successfully identified ANF mRNA. Similar to cultured kidney epithelial cells, normal rat kidney expresses ANF mRNA, but at a very low abundance, thus necessitating two rounds of PCR amplification. Further characterization of rat cortical distal tubular epithelia poly(A)+ RNA by Northern analysis revealed two ANF gene transcripts. A 1.0-kb message that comigrated with rat atrial ANF mRNA, and a second larger 1.4-kb transcript. These studies further substantiate the synthesis of ANF in the mammalian kidney. Unlike the mammalian heart, the kidney contains two ANF gene transcripts.

scholarly journals Chlorpromazine Induces Basolateral Aquaporin-2 Accumulation via F-Actin Depolymerization and Blockade of Endocytosis in Renal Epithelial Cells

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 1057
Author(s):  
Richard Bouley ◽  
Naofumi Yui ◽  
Abby Terlouw ◽  
Pui W. Cheung ◽  
Dennis Brown
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Mdck Cells ◽  
Rat Kidney ◽  
Apical Plasma Membrane ◽  
Rhodamine Phalloidin ◽  
Renal Epithelial Cells ◽  
Aquaporin 2 ◽  
Basolateral Plasma Membrane

We previously showed that in polarized Madin–Darby canine kidney (MDCK) cells, aquaporin-2 (AQP2) is continuously targeted to the basolateral plasma membrane from which it is rapidly retrieved by clathrin-mediated endocytosis. It then undertakes microtubule-dependent transcytosis toward the apical plasma membrane. In this study, we found that treatment with chlorpromazine (CPZ, an inhibitor of clathrin-mediated endocytosis) results in AQP2 accumulation in the basolateral, but not the apical plasma membrane of epithelial cells. In MDCK cells, both AQP2 and clathrin were concentrated in the basolateral plasma membrane after CPZ treatment (100 µM for 15 min), and endocytosis was reduced. Then, using rhodamine phalloidin staining, we found that basolateral, but not apical, F-actin was selectively reduced by CPZ treatment. After incubation of rat kidney slices in situ with CPZ (200 µM for 15 min), basolateral AQP2 and clathrin were increased in principal cells, which simultaneously showed a significant decrease of basolateral compared to apical F-actin staining. These results indicate that clathrin-dependent transcytosis of AQP2 is an essential part of its trafficking pathway in renal epithelial cells and that this process can be inhibited by selectively depolymerizing the basolateral actin pool using CPZ.

scholarly journals The metalloproteinase matrilysin is preferentially expressed by epithelial cells in a tissue-restricted pattern in the mouse.

1995 ◽  
Vol 6 (7) ◽  
pp. 851-869 ◽  
Author(s):  
C L Wilson ◽  
K J Heppner ◽  
L A Rudolph ◽  
L M Matrisian
Keyword(s):  
Polymerase Chain Reaction ◽  
Small Intestine ◽  
Epithelial Cells ◽  
Early Time ◽  
Reproductive Organs ◽  
Northern Analysis ◽  
Chain Reaction ◽  
Adult Mice ◽  
The Matrix ◽  
Polymerase Chain

To explore the role of the matrix metalloproteinase matrilysin (MAT) in normal tissue remodeling, we cloned the murine homologue of MAT from postpartum uterus using RACE polymerase chain reaction and examined its pattern of expression in embryonic, neonatal, and adult mice. The murine coding sequence and the corresponding predicted protein sequence were found to be 75% and 70% identical to the human sequences, respectively, and organization of the six exons comprising the gene is similar to the human gene. Northern analysis and in situ hybridization revealed that MAT is expressed in the normal cycling, pregnant, and postpartum uterus, with levels of expression highest in the involuting uterus at early time points (6 h to 1.5 days postpartum). The mRNA was confined to epithelial cells lining the lumen and some glandular structures. High constitutive levels of MAT transcripts were also detected in the small intestine, where expression was localized to the epithelial Paneth cells at the base of the crypts. Similarly, MAT expression was found in epithelial cells of the efferent ducts, in the initial segment and cauda of the epididymis, and in an extra-hepatic branch of the bile duct. MAT transcripts were detectable only by reverse transcription-polymerase chain reaction in the colon, kidney, lung, skeletal muscle, skin, stomach, juvenile uterus, and normal, lactating, and involuting mammary gland, as was expression primarily late in embryogenesis. Analysis of MAT expression during postnatal development indicated that although MAT is expressed in the juvenile small intestine and reproductive organs, the accumulation of significant levels of MAT mRNA appears to correlate with organ maturation. These results show that MAT expression is restricted to specific organs in the mouse, where the mRNA is produced exclusively by epithelial cells, and suggest that in addition to matrix degradation and remodeling, MAT may play an important role in the differentiated function of these organs.

Effect of intoxication and burn on claudin and occludin expression in epithelial cells from the small and large intestine

Alcohol ◽  
2014 ◽  
Vol 48 (7) ◽  
pp. 730
Author(s):  
A.M. Hammer ◽  
J.L. Rendon ◽  
X. Li ◽  
N.L. Morris ◽  
Z.M. Earley ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Large Intestine ◽  
Small And Large Intestine
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