Effects of long-term orall-arginine on esophageal motility and gallbladder dynamics in healthy humans

Inhibitory nitrergic neurons are known to play a role in the regulation of motility patterns of the distal esophagus, the lower esophageal sphincter (LES), and the gallbladder. Our study aim was to investigate the effects of “long-term” (i.e., prolonged) oral intake ofl-arginine (l-Arg), the endogenous source for nitric oxide (NO) synthesis, on postprandial LES pressure (LESP), esophageal motility, gastroesophageal reflux, and gallbladder motility. l-Arg (30 g/day) or glycine (placebo; 13 g/day; isosmolar) was given orally to 10 healthy male volunteers for 8 days, according to a randomized, crossover design. Twenty-four-hour urinary nitrite/nitrate excretion was measured to indicate NO synthesis. Basal early postprandial LESP was lower after l-Arg ingestion (2.2 kPa) than after glycine ingestion (2.7 kPa) ( P < 0.05).l-Arg abolished the physiological late postprandial rise in LESP. Transient LES relaxations were longer lasting after l-Arg ingestion ( P < 0.02). Esophageal motility and reflux were not affected (not significant). Fasting and residual gallbladder volumes were greater afterl-Arg ingestion ( P < 0.05). Urinary nitrite/nitrate excretion was higher after l-Arg intake ( P < 0.05). In conclusion, long-term oral l-Arg suppresses late postprandial LESP increase, prolongs transient LES relaxations, and increases fasting and residual gallbladder volumes. These effects may be mediated by increased NO synthesis.

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Faculty Opinions recommendation of Long-term plasticity of the spinal locomotor circuitry mediated by endocannabinoid and nitric oxide signaling.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1097590.553676 ◽

2008 ◽

Author(s):

Keith Sillar

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Signaling

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Faculty Opinions recommendation of Educational intervention in farmers with occupational asthma: long-term effect on exhaled nitric oxide.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1161657.623228 ◽

2009 ◽

Author(s):

Andrea Siracusa ◽

Ilenia Folletti

Keyword(s):

Nitric Oxide ◽

Educational Intervention ◽

Occupational Asthma ◽

Exhaled Nitric Oxide ◽

Term Effect ◽

Long Term Effect

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Hydrogen Sulfide in Physiological and Pathological Mechanisms in Brain

CNS & Neurological Disorders - Drug Targets ◽

10.2174/1871527317666180605072018 ◽

2018 ◽

Vol 17 (9) ◽

pp. 654-670 ◽

Cited By ~ 16

Author(s):

Mohit Kumar ◽

Rajat Sandhir

Keyword(s):

Nitric Oxide ◽

Traumatic Brain Injury ◽

Hydrogen Sulfide ◽

Neurodegenerative Disorders ◽

Neurological Disorders ◽

Scientific Community ◽

Molecular Targets ◽

Long Term Potentiation ◽

Term Potentiation

Background & Objective: Hydrogen sulfide [H2S] has been widely known as a toxic gas for more than 300 years in the scientific community. However, the understanding about this small molecule has changed after the discovery of involvement of H2S in physiological and pathological mechanisms in brain. H2S is a third gasotransmitter and neuromodulator after carbon monoxide [CO] and nitric oxide [NO]. H2S plays an important role in memory and cognition by regulating long-term potentiation [LTP] and calcium homeostasis in neuronal cells. The disturbances in endogenous H2S levels and trans-sulfuration pathway have been implicated in neurodegenerative disorders like Alzheimer’s disease, Parkinson disease, stroke and traumatic brain injury. According to the results obtained from various studies, H2S not only behaves as neuromodulator but also is a potent antioxidant, anti-inflammatory and anti-apoptotic molecule suggesting its neuroprotective potential. Conclusion: Recently, there is an increased interest in developing H2S releasing pharmaceuticals to target various neurological disorders. This review covers the information about the involvement of H2S in neurodegenerative diseases, its molecular targets and its role as potential therapeutic molecule.

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Effects of Long-term Nitric Oxide Synthesis Inhibition on Plasma Volume Expansion and Fetal Growth in the Pregnant Rat

Hypertension ◽

10.1161/01.hyp.26.6.1019 ◽

1995 ◽

Vol 26 (6) ◽

pp. 1019-1023 ◽

Cited By ~ 45

Author(s):

Sofía P. Salas ◽

Fernando Altermatt ◽

Mauricio Campos ◽

Andrea Giacaman ◽

Pedro Rosso

Keyword(s):

Nitric Oxide ◽

Fetal Growth ◽

Plasma Volume ◽

Volume Expansion ◽

Nitric Oxide Synthesis ◽

Plasma Volume Expansion ◽

Pregnant Rat ◽

Synthesis Inhibition

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A metal-organic-framework incorporated vascular graft for sustained nitric oxide generation and long-term vascular patency

Chemical Engineering Journal ◽

10.1016/j.cej.2021.129577 ◽

2021 ◽

pp. 129577

Author(s):

Xiangyun Zhang ◽

Yuanbo Wang ◽

Jing Liu ◽

Jie Shi ◽

Duo Mao ◽

...

Keyword(s):

Nitric Oxide ◽

Vascular Graft ◽

Metal Organic Framework ◽

Metal Organic ◽

Vascular Patency ◽

Organic Framework

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Cold Atmospheric Pressure Plasma-Activated Medium Induces Selective Cell Death in Human Hepatocellular Carcinoma Cells Independently of Singlet Oxygen, Hydrogen Peroxide, Nitric Oxide and Nitrite/Nitrate

International Journal of Molecular Sciences ◽

10.3390/ijms22115548 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5548

Author(s):

Yan Li ◽

Tianyu Tang ◽

Haejune Lee ◽

Kiwon Song

Keyword(s):

Nitric Oxide ◽

Cell Death ◽

Singlet Oxygen ◽

Atmospheric Pressure ◽

Atmospheric Pressure Plasma ◽

Proliferative Effect ◽

Huh7 Cells ◽

Anti Cancer ◽

Cold Atmospheric Pressure Plasma ◽

Nitrite Nitrate

Cold atmospheric pressure plasma (CAP) and plasma-activated medium (PAM) induce cell death in diverse cancer cells and may function as powerful anti-cancer agents. The main components responsible for the selective anti-cancer effects of CAP and PAM remain elusive. CAP or PAM induces selective cell death in hepatocellular carcinoma cell lines Hep3B and Huh7 containing populations with cancer stem cell markers. Here, we investigated the major component(s) of CAP and PAM for mediating the selective anti-proliferative effect on Hep3B and Huh7 cells. The anti-proliferative effect of CAP was mediated through the medium; however, the reactive oxygen species scavenger N-acetyl cysteine did not suppress PAM-induced cell death. Neither high concentrations of nitrite or nitrite/nitrate nor a low concentration of H2O2 present in the PAM containing sodium pyruvate affected the viability of Hep3B and Huh7 cells. Inhibitors of singlet oxygen, superoxide anions, and nitric oxide retained the capacity of PAM to induce anti-cancer effects. The anti-cancer effect was largely blocked in the PAM prepared by placing an aluminum metal mesh, but not a dielectric PVC mesh, between the plasma source and the medium. Hence, singlet oxygen, hydrogen peroxide, nitric oxide, and nitrite/nitrate are not the main factors responsible for PAM-mediated selective death in Hep3B and Huh7 cells. Other factors, such as charged particles including various ions in CAP and PAM, may induce selective anti-cancer effects in certain cancer cells.

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How to Cope with Big Data in Functional Analysis of the Esophagus

Visceral Medicine ◽

10.1159/000511931 ◽

2020 ◽

Vol 36 (6) ◽

pp. 439-442

Author(s):

Alissa Jell ◽

Christina Kuttler ◽

Daniel Ostler ◽

Norbert Hüser

Keyword(s):

Esophageal Motility ◽

Learning Algorithm ◽

Supervised Machine Learning ◽

Esophageal Motility Disorders ◽

Motility Disorders ◽

High Resolution Manometry ◽

Evaluation Time ◽

Core Evaluation

Introduction: Esophageal motility disorders have a severe impact on patients’ quality of life. While high-resolution manometry (HRM) is the gold standard in the diagnosis of esophageal motility disorders, intermittently occurring muscular deficiencies often remain undiscovered if they do not lead to an intense level of discomfort or cause suffering in patients. Ambulatory long-term HRM allows us to study the circadian (dys)function of the esophagus in a unique way. With the prolonged examination period of 24 h, however, there is an immense increase in data which requires personnel and time for evaluation not available in clinical routine. Artificial intelligence (AI) might contribute here by performing an autonomous analysis. Methods: On the basis of 40 previously performed and manually tagged long-term HRM in patients with suspected temporary esophageal motility disorders, we implemented a supervised machine learning algorithm for automated swallow detection and classification. Results: For a set of 24 h of long-term HRM by means of this algorithm, the evaluation time could be reduced from 3 days to a core evaluation time of 11 min for automated swallow detection and clustering plus an additional 10–20 min of evaluation time, depending on the complexity and diversity of motility disorders in the examined patient. In 12.5% of patients with suggested esophageal motility disorders, AI-enabled long-term HRM was able to reveal new and relevant findings for subsequent therapy. Conclusion: This new approach paves the way to the clinical use of long-term HRM in patients with temporary esophageal motility disorders and might serve as an ideal and clinically relevant application of AI.

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The Long-Term Effects of Oral and Transdermal Postmenopausal Hormone Replacement Therapy on Nitric Oxide, Endothelin-1, Prostacyclin, and Thromboxane

Fertility and Sterility ◽

10.1016/s0015-0282(98)00028-4 ◽

1998 ◽

Vol 69 (5) ◽

pp. 883-888 ◽

Cited By ~ 50

Author(s):

O Ylikorkala

Keyword(s):

Nitric Oxide ◽

Hormone Replacement Therapy ◽

Replacement Therapy ◽

Hormone Replacement ◽

Long Term Effects ◽

Postmenopausal Hormone ◽

Endothelin 1 ◽

Postmenopausal Hormone Replacement Therapy

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Effects of Early Neuronal and Delayed Inducible Nitric Oxide Synthase Blockade on Cardiovascular, Renal, and Hepatic Function in Ovine Sepsis

Anesthesiology ◽

10.1097/aln.0b013e3181fc5588 ◽

2010 ◽

Vol 113 (6) ◽

pp. 1376-1384 ◽

Cited By ~ 14

Author(s):

Matthias Lange ◽

Atsumori Hamahata ◽

Daniel L. Traber ◽

Yoshimitsu Nakano ◽

Aimalohi Esechie ◽

...

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Inducible Nitric Oxide Synthase ◽

Nitric Oxide Synthase Inhibitor ◽

Nitrite Nitrate ◽

Synthase Inhibitor ◽

Plasma Nitrite ◽

Oxide Synthase ◽

Organ Dysfunctions ◽

Inducible Nitric Oxide

Background Recent evidence suggests that nitric oxide produced via the neuronal nitric oxide synthase is involved mainly in the early response to sepsis, whereas nitric oxide derived from the inducible nitric oxide synthase is responsible during the later phase. We hypothesized that early neuronal and delayed inducible nitric oxide synthase blockade attenuates multiple organ dysfunctions during sepsis. Methods Sheep were randomly allocated to sham-injured, nontreated animals (n = 6); injured (48 breaths of cotton smoke and instillation of Pseudomonas aeruginosa into the lungs), nontreated animals (n = 7); and injured animals treated with a neuronal nitric oxide synthase inhibitor from 1 to 12 h and an inducible nitric oxide synthase inhibitor from 12 to 24 h postinjury (n = 6). Results The injury induced arterial hypotension, vascular leakage, myocardial depression, and signs of renal and hepatic dysfunctions. The treatment significantly attenuated, but did not fully prevent, the decreases in mean arterial pressure and left ventricular stroke work index. Although the elevation of creatinine levels was partially prevented, the decreases in urine output and creatinine clearance were not affected. The injury-related increases in bilirubin levels, international normalized ratio, and lipid peroxidation in liver tissue were significantly attenuated. Although plasma nitrite/nitrate levels were significantly increased versus baseline from 12-24 h in controls, plasma nitrite/nitrate levels were not increased in treated animals. Conclusions The combination treatment shows potential benefit on sepsis-related arterial hypotension and surrogate parameters of organ dysfunctions in sheep. It may be crucial to identify the time course of expression and activation of different nitric oxide synthase isoforms in future investigations.

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