Interaction of neutrophils and endothelium in isolated coronary venules and arterioles

1995 ◽  
Vol 268 (1) ◽  
pp. H490-H498 ◽  
Author(s):  
Y. Yuan ◽  
R. A. Mier ◽  
W. M. Chilian ◽  
D. C. Zawieja ◽  
H. J. Granger
Keyword(s):  
Flow Velocity ◽  
Shear Force ◽  
Low Flow ◽  
Microvascular Endothelium ◽  
Neutrophil Adherence ◽  
Flow Velocities

This study reports measurements of porcine neutrophil dynamics in isolated microvessels. Porcine coronary venules and arterioles were isolated, cannulated, and perfused with fluorescently labeled neutrophils at a series of flow velocities. In venules (62.50 +/- 5.41 microns diam) under control conditions, rolling neutrophils were often observed at intraluminal flow velocities ranging from 600 to 6,000 microns/s, and the rolling fraction varied inversely as a function of flow velocity. There was no significant adherence under the control conditions at any of the various flow velocities. Pretreatment of the neutrophils with human recombinant complement 5a (C5a, 10(-8) M) increased adherence at low flow velocities but did not alter the rolling fraction. In contrast to venules, rolling neutrophils were not observed in arterioles (58.80 +/- 5.6 microns diam). Furthermore, neutrophils that were pretreated with C5a did not adhere to the arteriolar endothelium even at low flow velocities. We suggest that 1) isolated microvessels perfused with fluorescently labeled neutrophils are suitable models for the study of the interaction between neutrophils and the microvascular endothelium, 2) shear force plays an important role in neutrophil rolling in coronary venules but is not the major factor that prevents neutrophil rolling and adherence in arterioles, and 3) C5a causes neutrophil adherence in venules but not in arterioles, indicating that different mechanisms underlie the interaction between neutrophils and endothelium in venules and arterioles.

Surface Radiation Effects on Flame Spread Over Thermally Thick Fuels in an Opposing Flow

1994 ◽  
Vol 116 (3) ◽  
pp. 646-651 ◽  
Author(s):  
J. West ◽  
S. Bhattacharjee ◽  
R. A. Altenkirch
Keyword(s):  
Flow Velocity ◽  
Gas Phase ◽  
Flame Spread ◽  
Radiation Effects ◽  
Solid Phase ◽  
Surface Radiation ◽  
Low Flow ◽  
Fuel Surface ◽  
Wide Range ◽  
Flow Velocities

A computational model of flame spread over a thermally thick solid fuel in an opposing-flow environment is presented. Unlike thermally thin fuels, for which the effect of fuel surface radiation is negligible for high levels of opposing flow, fuel surface radiation is important for thermally thick fuels for all flow levels. This result is shown to derive from the fact that the ratio of the rate of heat transfer by re-radiation from the surface to that by conduction from the gas to the solid is proportional to the length over which heat can be conducted forward of the flame to sustain spreading. For thin fuels, this length decreases with increasing flow velocity such that while radiation is important at low flow velocities it is not at the higher velocities. For thick fuels at low flow velocities, the conduction length is determined by gas-phase processes and decreases with increasing flow velocity. But at higher flow velocities, the conduction length is determined by solid-phase processes and is rather independent of the gas-phase flow. The result is that over a wide range of flow velocities, the conduction length of importance does not change substantially as it switches from one phase to another so that the ratio of radiation to conduction is of unit order throughout that wide range of flow.

scholarly journals Relative impacts of the invasive Pacific oyster, Crassostrea gigas, over the native blue mussel, Mytilus edulis, are mediated by flow velocity and food concentration

NeoBiota ◽  
2019 ◽  
Vol 45 ◽  
pp. 19-37 ◽  
Author(s):  
Patrick W.S. Joyce ◽  
Louise Kregting ◽  
Jaimie T.A. Dick
Keyword(s):  
Flow Velocity ◽  
Pacific Oyster ◽  
Food Concentration ◽  
Ecological Impacts ◽  
Low Flow ◽  
Relative Impact ◽  
Clearance Rates ◽  
Mussel Beds ◽  
Food Concentrations ◽  
Flow Velocities

The ecological impacts of invasive species can be severe, but are generally viewed as highly unpredictable. Recent methods combining per capita feeding rates, population abundances and environmental contexts have shown great utility in predicting invader impacts. Here, clearance rates of the invasive Pacific oyster, Crassostreagigas, and native mussel, Mytilusedulis, were investigated in a laboratory experiment where oscillatory water flow and algal food concentrations were manipulated. Invasive oysters had lower clearance rates than native mussels in all experimental groups and did not differ among flow velocities or food concentrations. Native mussel clearance rates were higher at 5 cm s-1 compared to 0 and 15 cm s-1 flow velocities and increased with increasing food concentration. The Relative Impact Potential (RIP) metric was used to assess (i) the influence of flow velocity and food concentration on potential impacts of C.gigas on plankton resources and, (ii) the impacts of coexisting reefs, containing both species, on resources compared to monospecific native mussel beds. Greatest Relative Impact Potential of invasive oysters was seen at the lowest flow velocity, but became reduced with increasing flow velocity and food concentration. Relative Impact Potentials of coexisting reefs were generally greater than monospecific native mussel beds, with greatest impacts predicted at lowest flow velocity. We suggest that the greatest ecological impacts and competition potential of C.gigas will occur in areas with low flow velocity, but that increased flow will mediate co-existence between the two species.

Experimental Investigation of the Acoustic Power Around Two Tandem Cylinders

2010 ◽  
Vol 132 (4) ◽  
Author(s):  
S. L. Finnegan ◽  
C. Meskell ◽  
S. Ziada
Keyword(s):  
Flow Field ◽  
Flow Velocity ◽  
Vortex Shedding ◽  
Acoustic Resonance ◽  
Low Flow ◽  
Interaction Mechanisms ◽  
Tandem Cylinders ◽  
Vortex Shedding Frequency ◽  
Shedding Frequency ◽  
Flow Velocities

Aeroacoustic resonance of bluff bodies exposed to cross flow can be problematic for many different engineering applications and knowledge of the location and interaction of acoustic sources is not well understood. Thus, an empirical investigation of the acoustically coupled flow around two tandem cylinders under two different resonant conditions is presented. It is assumed that the resonant acoustic field could be decoupled from the hydrodynamic flow field, resolved separately, and then recoupled to predict the flow/sound interaction mechanisms using Howe's theory of aerodynamic sound. Particle image velocimetry was employed to resolve the phase-averaged flow field characteristics around the cylinders at various phases in an acoustic wave cycle. It was found that the vortex shedding patterns of the two resonant conditions exhibit substantial differences. For the first condition, which occurred at low flow velocities where the natural vortex shedding frequency was below the acoustic resonance frequency, fully developed vortices formed in both the gap region between the cylinders and in the wake. These vortices were found to be in phase with each other. For the second resonant condition, which occurred at higher flow velocities where the natural vortex shedding frequency was above the acoustic resonant frequency, fully developed vortices only formed in the wake and shedding from the two cylinders were not in phase. These differences in the flow field resulted in substantial variation in the flow-acoustic interaction mechanisms between the two resonant conditions. Corresponding patterns of the net acoustic energy suggest that acoustic resonance at the lower flow velocity is due to a combination of shear layer instability in the gap and vortex shedding in the wake, while acoustic resonance at the higher flow velocity is driven by the vortex shedding in the wake of the two cylinders.

Effect of flow velocity on phosphate release from sediment

1994 ◽  
Vol 30 (10) ◽  
pp. 263-272 ◽  
Author(s):  
Yoshiyuki Nakamura
Keyword(s):  
Flow Velocity ◽  
Release Rate ◽  
Bulk Water ◽  
Low Flow ◽  
Limiting Factor ◽  
Phosphate Release ◽  
Release Flux ◽  
Diffusive Boundary ◽  
Do Concentration ◽  
Flow Velocities

A mathematical model of phosphate release rate from sediment, fp, is presented which determines the fp as a function of flow velocity over the sediment and dissolved oxygen concentration. Oxygen consumption in the sediment is expressed as the sum of chemical consumption due to ferrous iron oxygenation and the bacterial consumption which is assumed to be a first order reaction of oxygen. At very low flow velocities, transport through the diffusive boundary layer is the limiting factor of SOD, and phosphate release rate is expressed as a linear decreasing function of the velocity. When flow velocities are increased, both SOD and phosphate release rate become independent of velocity, since the reactions in the sediment are the rate limiting factor. The model suggests that phosphate release flux is a linear decreasing function of DO in the bulk water, while SOD is an increasing function of DO concentration. The critical DO concentration at which the phosphate release ceases is expressed in terms of the flow velocity. The prediction of SOD and ϕp by the present model is favourably compared with experiments by former researchers.

scholarly journals Optical Micro-Wire Flow-Velocity Sensor

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 4025
Author(s):  
Matej Njegovec ◽  
Simon Pevec ◽  
Denis Donlagic
Keyword(s):  
Response Time ◽  
Flow Velocity ◽  
Time Constant ◽  
Gas Flow ◽  
Low Flow ◽  
Velocity Sensor ◽  
Short Response Time ◽  
Wide Range ◽  
Cost Efficient ◽  
Flow Velocities

This paper presents a short response time, all-silica, gas-flow-velocity sensor. The active section of the sensor consists of a 16 µm diameter, highly optically absorbing micro-wire, which is heated remotely by a 980 nm light source. The heated microwire forms a Fabry–Perot interferometer whose temperature is observed at standard telecom wavelengths (1550 nm). The short response time of the sensor allows for different interrogation approaches. Direct measurement of the sensor’s thermal time constant allowed for flow-velocity measurements independent of the absolute heating power delivered to the sensor. This measurement approach also resulted in a simple and cost-efficient interrogation system, which utilized only a few telecom components. The sensor’s short response time, furthermore, allowed for dynamic flow sensing (including turbulence detection). The sensor’s bandwidth was measured experimentally and proved to be in the range of around 22 Hz at low flow velocities. Using time constant measurement, we achieved a flow-velocity resolution up to 0.006 m/s at lower flow velocities, while the resolution in the constant power configuration was better than 0.003 m/s at low flow velocities. The sensing system is constructed around standard telecommunication optoelectronic components, and thus suitable for a wide range of applications.

scholarly journals Near-occlusion is difficult to diagnose with common carotid ultrasound methods

2021 ◽  
Vol 63 (5) ◽  
pp. 721-730
Author(s):  
Elias Johansson ◽  
Davide Vanoli ◽  
Isa Bråten-Johansson ◽  
Lucy Law ◽  
Richard I Aviv ◽  
...  
Keyword(s):  
Flow Velocity ◽  
Sensitivity And Specificity ◽  
Low Flow ◽  
Reference Standard ◽  
Carotid Ultrasound ◽  
Ultrasound Method ◽  
Velocity Parameter ◽  
Near Occlusion

Abstract Purpose To assess the sensitivity and specificity of common carotid ultrasound method for carotid near-occlusion diagnosis. Methods Five hundred forty-eight patients examined with both ultrasound and CTA within 30 days of each other were analyzed. CTA graded by near-occlusion experts was used as reference standard. Low flow velocity, unusual findings, and commonly used flow velocity parameters were analyzed. Results One hundred three near-occlusions, 272 conventional ≥50% stenosis, 162 <50% stenosis, and 11 occlusions were included. Carotid ultrasound was 22% (95%CI 14–30%; 23/103) sensitive and 99% (95%CI 99–100%; 442/445) specific for near-occlusion diagnosis. Near-occlusions overlooked on ultrasound were found misdiagnosed as occlusions (n = 13, 13%), conventional ≥50% stenosis (n = 65, 63%) and < 50% stenosis (n = 2, 2%). No velocity parameter or combination of parameters could identify the 65 near-occlusions mistaken for conventional ≥50% stenoses with >75% sensitivity and specificity. Conclusion Near-occlusion is difficult to diagnose with commonly used carotid ultrasound methods. Improved carotid ultrasound methods are needed if ultrasound is to retain its position as sole preoperative modality.

Intraoperative transesophageal assessment of coronary artery flow can predict 5 type myocardial infarction

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
A Zagatina ◽  
M Novikov ◽  
N Zhuravskaya ◽  
V Balakhonov ◽  
S Efremov ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Surgery ◽  
Coronary Artery ◽  
Flow Velocity ◽  
Coronary Flow ◽  
Index Number ◽  
Left Main ◽  
Coronary Flow Velocity ◽  
Before And After ◽  
Flow Velocities

Abstract Background Stenosis of a coronary artery results in an increase in flow velocity in the pathologic segment. Effective grafting should decrease the stenotic native coronary velocity according to hemodynamic law. The range of decreased velocity before and after cardiac surgery can hypothetically reflect the effectiveness of a graft. The aim of the study is to determine if measuring coronary flow velocity changes during coronary artery bypass grafting (CABG) can predict intraoperative myocardial infarction. Methods One hundred sixty-six (166) consecutive patients (121 men, 64±9 years old) referred for cardiac surgery, were prospectively included in the study. A standard basic perioperative transesophageal echocardiography (TEE) examination was performed with additional scans of the left main, left anterior descending (LAD), and circumflex (LCx) arteries' proximal segments. Measurements of coronary flow velocities were performed before and after grafting in the same sites of the arteries. The maximal value of cardiac troponin I (cTnI) after CABG and the additive criteria were accounted for in the analysis as it is described in the expert consensus document for Type 5 myocardial infarction (MI) definition. Results One hundred sixty-three patients (98%) had arterial hypertension, 28 patients (17%) had diabetes mellitus, 35 patients (21%) were currently smokers. The feasibility of coronary flow assessment during cardiac operations was 95%. Before grafting, the mean velocity in the left main artery was 91±49 cm/s, in LAD 101±35 cm/s, and in LCx 117±49 cm/s. There was a significant correlation between changes in coronary flow velocities during operation and the value of cTnI (R=0.34, p&lt;0.0001). Ten patients met the criteria for Type 5 MI. There were no differences in age, body mass index, number of coronary arteries with stenoses, frequency of prior MI, ejection fraction or coronary flow velocity before surgery in patients with and without Type 5 MI. The group of patients with Type 5 MI had an increase in native artery velocities during surgery in comparison with patients without MI, who had a significant decrease in coronary flow velocity after grafting (30±48 vs. −10±30 cm/s; p&lt;0.0006). Increases in native coronary velocities greater than 3 cm/s predicted Type 5 MI with 81% accuracy (sensitivity 88%, specificity 70%). Conclusion Coronary flow velocity assessment during cardiac surgery could predict an elevation of cardiac troponins and Type 5 MI. Funding Acknowledgement Type of funding source: None

Neutrophil-dependent augmentation of PAF-induced vasoconstriction and albumin flux in coronary arterioles

1998 ◽  
Vol 275 (4) ◽  
pp. H1138-H1147 ◽  
Author(s):  
Qiaobing Huang ◽  
Mac Wu ◽  
Cynthia Meininger ◽  
Katherine Kelly ◽  
Yuan Yuan
Keyword(s):  
Platelet Activating Factor ◽  
Microvascular Dysfunction ◽  
Low Flow ◽  
Intercellular Adhesion Molecule ◽  
Neutrophil Adhesion ◽  
Intercellular Adhesion Molecule 1 ◽  
Apparent Permeability ◽  
Inflammatory Reactions ◽  
Coronary Arterioles ◽  
Flow Velocities

Platelet-activating factor (PAF) has been implicated in the pathogenesis of ischemic heart disease, reperfusion injury, and inflammatory reactions. Although neutrophils have been shown to primarily mediate PAF-induced microvascular dysfunction, the vasoactive effect of PAF and its neutrophil-dependent mechanism have not been directly and systematically studied in coronary resistance vessels. Therefore, the aim of this study was to examine the effects of PAF on coronary arteriolar function and neutrophil dynamics using an isolated and perfused microvessel preparation. Topical application of PAF to the vessels induced a dose-dependent decrease in the diameter but an increase in the apparent permeability coefficient of albumin. Disruption of the endothelium abolished the vasomotor response to PAF, and perfusion of neutrophils significantly augmented PAF-induced changes in vasomotor tone and permeability. Furthermore, the interaction between neutrophils and the endothelium was studied in the intact perfused coronary arterioles. Under control conditions, there were no adherent neutrophils observed in the vessels at varied intraluminal flow velocities. However, administration of PAF caused neutrophil adhesion to the endothelium of coronary arterioles at low flow velocities. Western blot analysis indicated that PAF upregulated the expression of intercellular adhesion molecule-1 in cultured coronary microvascular endothelial cells. Taken together, the results suggest that 1) PAF induces vasoconstriction and hyperpermeability in coronary arterioles via an endothelium-dependent and neutrophil-mediated mechanism, and 2) PAF is able to stimulate neutrophil adhesion in coronary arterioles under a condition of low flow rate.

Corrosion Rate Measurement by Hydrogen Effusion In Dynamic Aqueous Systems At Elevated Temperature and Pressure

CORROSION ◽  
1959 ◽  
Vol 15 (4) ◽  
pp. 29-32
Author(s):  
M. KRULFELD ◽  
M. C. BLOOM ◽  
R. E. SEEBOLD
Keyword(s):  
Elevated Temperature ◽  
Corrosion Rate ◽  
Flow Velocity ◽  
High Flow ◽  
Low Flow ◽  
Aqueous Systems ◽  
Effusion Rates ◽  
Temperature And Pressure ◽  
Effusion Method ◽  
Hydrogen Effusion

Abstract A method of applying the hydrogen effusion method to the measurement of corrosion rates in dynamic aqueous systems at elevated temperature and pressure is described. Data obtained in low carbon steel systems are presented, including (1) reproducibility obtained in measured hydrogen effusion rates at a flow velocity of 1 foot per second at a temperature of 600 F and 2000 psi, and (2) a quantitative comparison between the hydrogen effusion rates in static and in low flow velocity dynamic systems at this temperature and pressure. Some observations are included on corrosion rate measurements in a high flow velocity (30 feet per second) loop by the hydrogen effusion method. Implications of these measurements with regard to the comparison between high flow velocity corrosion and low flow velocity corrosion are mentioned and some data indicating high local sensitivity of the hydrogen effusion method are noted. Some possible difficulties involved in the method are pointed out. 2.3.4

Unidirectional to bidirectional subtidal sandwaves influenced by gradually decreasing steady flow velocity

1997 ◽  
Vol 134 (4) ◽  
pp. 557-561
Author(s):  
KATSUHIRO NAKAYAMA
Keyword(s):  
Grain Size ◽  
Flow Velocity ◽  
Steady Flow ◽  
Periodic Flow ◽  
Southwest Japan ◽  
Unit Thickness ◽  
Flow Components ◽  
Flow Velocities

Miocene subtidal sandwave deposits in southwest Japan were influenced by periodic flow and steady flow. The sandwave deposits can be divided into five units, based on lithofacies and thickness. In order of accretion, unit 1 consists of unidirectional sand bedforms without mud drapes, unit 2 of unidirectional sand bedforms with thin, discontinuous mud drapes, unit 3 of bidirectional sand bedforms with thin continuous mud drapes, and units 4 and 5 of relatively thinner and smaller bidirectional sand bedforms with continuous mud drapes. The thickness of units 1 to 3 increase progressively to 2.6 m, and units 4 to 5 subsequently decrease from 2.0 to 1.0 m. Variations between the units are due to differing combinations of periodic and steady flow velocities. Palaeoflow velocity is estimated from grain size and unit thickness. Depth-mean velocities of steady flow components gradually decrease from 0.72 ms−1 to 0.16 ms−1 with unit accumulation.

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