scholarly journals Alterations in myocardial collagen content affect rat papillary muscle function

2000 ◽  
Vol 279 (4) ◽  
pp. H1534-H1539 ◽  
Author(s):  
Luiz S. Matsubara ◽  
Beatriz B. Matsubara ◽  
Marina P. Okoshi ◽  
Antonio C. Cicogna ◽  
Joseph S. Janicki
Keyword(s):  
Papillary Muscle ◽  
Muscle Function ◽  
Volume Fraction ◽  
Systolic Function ◽  
Collagen Content ◽  
Passive Stiffness ◽  
Collagen Concentration ◽  
Cross Sectional ◽  
Rat Papillary Muscle ◽  
Myocardial Collagen

We investigated the influence of myocardial collagen volume fraction (CVF, %) and hydroxyproline concentration (μg/mg) on rat papillary muscle function. Collagen excess was obtained in 10 rats with unilateral renal ischemia for 5 wk followed by 3-wk treatment with ramipril (20 mg · kg−1· day−1) (RHTR rats; CVF = 3.83 ± 0.80, hydroxyproline = 3.79 ± 0.50). Collagen degradation was induced by double infusion of oxidized glutathione (GSSG rats; CVF = 2.45 ± 0.52, hydroxyproline = 2.85 ± 0.18). Nine untreated rats were used as controls (CFV = 3.04 ± 0.58, hydroxyproline = 3.21 ± 0.30). Active stiffness (AS; g · cm−2· % Lmax−1) and myocyte cross-sectional area (MA; μm2) were increased in the GSSG rats compared with controls [AS 5.86 vs. 3.96 ( P < 0.05); MA 363 ± 59 vs. 305 ± 28 ( P < 0.05)]. In GSSG and RHTR groups the passive tension-length curves were shifted downwards, indicating decreased passive stiffness, and upwards, indicating increased passive stiffness, respectively. Decreased collagen content induced by GSSG is related to myocyte hypertrophy, decreased passive stiffness, and increased AS, and increased collagen concentration causes myocardial diastolic dysfunction with no effect on systolic function.

Interstitial space and collagen alterations of the developing rat diaphragm

1993 ◽  
Vol 74 (5) ◽  
pp. 2450-2455 ◽  
Author(s):  
L. E. Gosselin ◽  
D. A. Martinez ◽  
A. C. Vailas ◽  
G. C. Sieck
Keyword(s):  
Postnatal Growth ◽  
Interstitial Space ◽  
Collagen Content ◽  
Diaphragm Muscle ◽  
Adult Males ◽  
Rat Diaphragm ◽  
Collagen Concentration ◽  
Cross Sectional ◽  
Hydroxyproline Concentration ◽  
Total Muscle

The effect of growth on the relative interstitial space [%total cross-sectional area (CSA)] and collagen content of the rat diaphragm muscle was examined at postnatal ages of 0, 7, 14, and 21 days as well as in adult males. The proportion of interstitial space relative to total muscle CSA was determined by computerized image analysis of lectin-stained cross sections of diaphragm muscle. To assess collagen content and extent of collagen maturation (i.e., cross-linking), high-pressure liquid chromatography analysis was used to measure hydroxyproline concentration and the nonreducible collagen cross-link hydroxylysylpyridinoline (HP), respectively. At birth, interstitial space accounted for approximately 47% of total diaphragm muscle CSA. During postnatal growth, the relative contribution of interstitial space decreased such that by adulthood the interstitial space accounted for approximately 18% of total muscle CSA. The change in relative interstitial space occurred without a concomitant change in hydroxyproline concentration. However, the concentration of HP markedly increased with age such that the adult diaphragm contained approximately 17 times more HP than at birth. These results indicate that during development the relative CSA occupied by interstitial space decreases as muscle fiber size increases. However, the reduction in relative interstitial space is not associated with a change in collagen concentration. Thus collagen density in the interstitial space may increase with age. It is possible that the observed changes in relative interstitial space and collagen influence the passive length-force properties of the diaphragm.

scholarly journals Ventricular remodeling induced by retinoic acid supplementation in adult rats

2003 ◽  
Vol 284 (6) ◽  
pp. H2242-H2246 ◽  
Author(s):  
Sergio Alberto Rupp de Paiva ◽  
Leonardo Antonio Mamede Zornoff ◽  
Marina Politi Okoshi ◽  
Katashi Okoshi ◽  
Luiz Shiguero Matsubara ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Papillary Muscle ◽  
Ventricular Remodeling ◽  
Volume Fraction ◽  
Maximum Velocity ◽  
Left Ventricular ◽  
Control Group ◽  
Adult Rats ◽  
Cross Sectional ◽  
Morphological Studies

Retinoic acid (RA) plays a role in regulating cardiac geometry and function throughout life. The aim of this study was to analyze the cardiac effects of RA in adult rats. Wistar rats were randomly allocated to a control group ( n = 18) receiving standard rat chow and a group treated with RA ( n = 14) receiving standard rat chow supplemented with RA for 90 days. All animals were evaluated by echocardiography, isolated papillary muscle function, and morphological studies. Whereas the RA-treated group developed an increase in both left ventricular (LV) mass and LV end-diastolic diameter, the ratio of LV wall thickness to LV end-diastolic diameter remained unchanged when compared with the control group. In the isolated papillary muscle preparation, RA treatment decreased the time to peak developed tension and increased the maximum velocity of isometric relengthening, indicating that systolic and diastolic function was improved. Although RA treatment produced an increase in myocyte cross-sectional area, the myocardial collagen volume fraction was similar to controls. Thus our study demonstrates that small physiological doses of RA induce ventricular remodeling resembling compensated volume-overload hypertrophy in rats.

The stabilization of B.C.C. Cu in Cu/Nb nanolayered composites

1996 ◽  
Vol 54 ◽  
pp. 228-229
Author(s):  
H. Kung ◽  
A.J. Griffin ◽  
Y.C. Lu ◽  
K.E. Sickafus ◽  
T.E. Mitchell ◽  
...  
Keyword(s):  
Electrical Resistivity ◽  
Layer Thickness ◽  
Volume Fraction ◽  
Ion Beam ◽  
High Strength ◽  
Cross Sectional ◽  
Metastable Structure ◽  
High Resolution Tem ◽  
Potential Improvement ◽  
Two Phases

Materials with compositionally modulated structures have gained much attention recently due to potential improvement in electrical, magnetic and mechanical properties. Specifically, Cu-Nb laminate systems have been extensively studied mainly due to the combination of high strength, and superior thermal and electrical conductivity that can be obtained and optimized for the different applications. The effect of layer thickness on the hardness, residual stress and electrical resistivity has been investigated. In general, increases in hardness and electrical resistivity have been observed with decreasing layer thickness. In addition, reduction in structural scale has caused the formation of a metastable structure which exhibits uniquely different properties. In this study, we report the formation of b.c.c. Cu in highly textured Cu/Nb nanolayers. A series of Cu/Nb nanolayered films, with alternating Cu and Nb layers, were prepared by dc magnetron sputtering onto Si {100} wafers. The nominal total thickness of each layered film was 1 μm. The layer thickness was varied between 1 nm and 500 nm with the volume fraction of the two phases kept constant at 50%. The deposition rates and film densities were determined through a combination of profilometry and ion beam analysis techniques. Cross-sectional transmission electron microscopy (XTEM) was used to examine the structure, phase and grain size distribution of the as-sputtered films. A JEOL 3000F high resolution TEM was used to characterize the microstructure.

scholarly journals Modeling of Bridging Law for Bundled Aramid Fiber-Reinforced Cementitious Composite and its Adaptability in Crack Width Evaluation

Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 179
Author(s):  
Daiki Sunaga ◽  
Takumi Koba ◽  
Toshiyuki Kanakubo
Keyword(s):  
Fiber Orientation ◽  
Crack Width ◽  
Volume Fraction ◽  
Characteristic Point ◽  
Crack Opening ◽  
Aramid Fiber ◽  
Cementitious Composite ◽  
Fiber Reinforced ◽  
Cross Sectional ◽  
Bridging Law

Tensile performance of fiber-reinforced cementitious composite (FRCC) after first cracking is characterized by fiber-bridging stress–crack width relationships called bridging law. The bridging law can be calculated by an integral calculus of forces carried by individual fibers, considering the fiber orientation. The objective of this study was to propose a simplified model of bridging law for bundled aramid fiber, considering fiber orientation for the practical use. By using the pullout characteristic of bundled aramid fiber obtained in the previous study, the bridging laws were calculated for various cases of fiber orientation. The calculated results were expressed by a bilinear model, and each characteristic point is expressed by the function of fiber-orientation intensity. After that, uniaxial tension tests of steel reinforced aramid-FRCC prism specimens were conducted to obtain the crack-opening behavior and confirm the adaptability of the modeled bridging laws in crack-width evaluation. The experimental parameters are cross-sectional dimensions of specimens and volume fraction of fiber. The test results are compared with the theoretical curves calculated by using the modeled bridging law and show good agreements in each parameter.

Ageing Changes in the Tensile Properties of Tendons: Influence of Collagen Fibril Volume Fraction

2008 ◽  
Vol 130 (2) ◽  
Author(s):  
K. L. Goh ◽  
D. F. Holmes ◽  
H.-Y. Lu ◽  
S. Richardson ◽  
K. E. Kadler ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Collagen Fibril ◽  
Volume Fraction ◽  
Area Fraction ◽  
Sectional Area ◽  
Cross Sectional ◽  
P Values ◽  
Age Related ◽  
Fibril Diameter ◽  
Age Related Changes

Connective tissues are biological composites comprising of collagen fibrils embedded in (and reinforcing) the hydrated proteoglycan-rich (PG) gel within the extracellular matrices (ECMs). Age-related changes to the mechanical properties of tissues are often associated with changes to the structure of the ECM, namely, fibril diameter. However, quantitative attempts to correlate fibril diameter to mechanical properties have yielded inconclusive evidence. Here, we described a novel approach that was based on the rule of mixtures for fiber composites to evaluate the dependence of age-related changes in tendon tensile strength (σ) and stiffness (E) on the collagen fibril cross-sectional area fraction (ρ), which is related to the fibril volume fraction. Tail tendons from C57BL6 mice from age groups 1.6–35.3months old were stretched to failure to determine σ and E. Parallel measurements of ρ as a function of age were made using transmission electron microscopy. Mathematical models (rule of mixtures) of fibrils reinforcing a PG gel in tendons were used to investigate the influence of ρ on ageing changes in σ and E. The magnitudes of σ, E, and ρ increased rapidly from 1.6monthsto4.0months (P-values <0.05) before reaching a constant (age independent) from 4.0monthsto29.0months (P-values >0.05); this trend continued for E and ρ (P-values >0.05) from 29.0monthsto35.3months, but not for σ, which decreased gradually (P-values <0.05). Linear regression analysis revealed that age-related changes in σ and E correlated positively to ρ (P-values <0.05). Collagen fibril cross-sectional area fraction ρ is a significant predictor of ageing changes in σ and E in the tail tendons of C57BL6 mice.

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