Use of arterialized venous blood sampling during incremental exercise tests

1992 ◽  
Vol 73 (3) ◽  
pp. 937-940 ◽  
Author(s):  
P. McLoughlin ◽  
P. Popham ◽  
R. A. Linton ◽  
R. C. Bruce ◽  
D. M. Band
Keyword(s):  
Exercise Test ◽  
Venous Blood ◽  
Normal Subjects ◽  
Work Load ◽  
Cycle Ergometer ◽  
Incremental Exercise ◽  
Exercise Tests ◽  
Incremental Exercise Test ◽  
Arterial Ph ◽  
Incremental Testing

Close agreement between arterialized venous and arterial pH, PCO2, and lactate has previously been demonstrated during steady-state exercise. The purpose of the present study was to compare arterialized venous and arterial pH, PCO2, K+, lactate, pyruvate, and epinephrine during the constantly changing circumstances of an incremental exercise test. Eight normal subjects undertook an incremental exercise test (increasing by 20 W/min) to exhaustion on a cycle ergometer during which simultaneous arterial and arterialized venous samples were drawn over the last 20 s of each work load. Linear regression of arterialized venous on arterial values showed that r varied from 0.97 to 0.99 for the variables examined and, therefore, showed that accurate estimates of arterial values could be made from the arterialized venous results during incremental testing. For many purposes it could be assumed that arterialized venous values equaled arterial values without serious error.

scholarly journals Comparison of the Ramp and Step Incremental Exercise Test Protocols in Assessing the Maximal Fat Oxidation Rate in Youth Cyclists

2021 ◽  
Vol 80 (1) ◽  
pp. 163-172
Author(s):  
Kamil Michalik ◽  
Natalia Danek ◽  
Marek Zatoń
Keyword(s):  
Oxygen Uptake ◽  
Exercise Test ◽  
Oxidation Rate ◽  
Fat Oxidation ◽  
Incremental Exercise ◽  
Step Test ◽  
Exercise Tests ◽  
Incremental Exercise Test ◽  
Ramp Test ◽  
Maximal Fat Oxidation

Abstract The incremental exercise test is the most common method in assessing the maximal fat oxidation (MFO) rate. The main aim of the study was to determine whether the progressive linear RAMP test can be used to assess the maximal fat oxidation rate along with the intensities that trigger its maximal (FATmax) and its minimal (FATmin) values. Our study comprised 57 young road cyclists who were tested in random order. Each of them was submitted to two incremental exercise tests on an electro-magnetically braked cycle-ergometer - STEP (50 W·3 min-1) and RAMP (~0.278 W·s-1) at a 7-day interval. A stoichiometric equation was used to calculate the fat oxidation rate, while the metabolic thresholds were defined by analyzing ventilation gases. The Student’s T-test, Bland-Altman plots and Pearson’s linear correlations were resorted to in the process of statistical analysis. No statistically significant MFO variances occurred between the tests (p = 0.12) and its rate amounted to 0.57 ± 0.15 g·min-1 and 0.53 ± 0.17 g·min-1 in the STEP and RAMP, respectively. No statistically significant variances in the absolute and relative (to maximal) values of oxygen uptake and heart rate were discerned at the FATmax and FATmin intensities. The RAMP test displayed very strong oxygen uptake correlations between the aerobic threshold and FATmax (r = 0.93, R2 = 0.87, p < 0.001) as well as the anaerobic threshold and FATmin (r = 0.88, R2 = 0.78, p < 0.001). Our results corroborate our hypothesis that the incremental RAMP test as well as the STEP test are reliable tools in assessing MFO, FATmax and FATmin intensities.

Lactate acidosis and the increase in VE/VO2 during incremental exercise

1987 ◽  
Vol 62 (4) ◽  
pp. 1551-1555 ◽  
Author(s):  
S. W. Farrell ◽  
J. L. Ivy
Keyword(s):  
Blood Lactate ◽  
Venous Blood ◽  
Experimental Treatment ◽  
Cycle Ergometer ◽  
Work Rate ◽  
Incremental Exercise ◽  
Exercise Tests ◽  
Similar Work ◽  
Blood Ph ◽  
Lactate Acidosis

The purpose of this investigation was to determine whether the onset of lactate acidosis is responsible for the increase in ventilatory equivalent (VE/VO2) during exercise of increasing intensity. Eight male subjects performed maximal incremental exercise tests on a cycle ergometer on two separate occasions. For the control (C) treatment, the initial work rates consisted of 4 min of unloaded pedaling (60 rpm) and 1 min of pedaling at a work rate of 30 W. Thereafter, the work rate was increased each minute by 22 W until volitional fatigue. Venous blood samples were taken before the onset of exercise and at the end of each work rate for determination of pH and lactate. Ventilatory parameters at each work rate were also monitored. Before the experimental treatment (E), the subjects performed two 3-min work bouts at high intensity (210–330 W) on the cycle ergometer in order to prematurely raise blood lactate levels and lower blood pH. The same incremental exercise test as C was then performed. The results indicated that the increase in VE/VO2 occurred at similar work rates and %VO2max although the venous H+ and lactate concentrations were significantly elevated during the E treatment. These results suggest that a decrease in the blood pH resulting from blood lactate accumulation is not responsible for the increase in VE/VO2 during incremental exercise.

Acid-base regulation during exercise and recovery in humans

1992 ◽  
Vol 72 (3) ◽  
pp. 954-961 ◽  
Author(s):  
W. Stringer ◽  
R. Casaburi ◽  
K. Wasserman
Keyword(s):  
Respiratory Acidosis ◽  
Normal Subjects ◽  
Cycle Ergometer ◽  
Moderate Intensity ◽  
Moderate Intensity Exercise ◽  
Exercise Tests ◽  
Cycle Ergometer Exercise ◽  
Arterial Ph ◽  
Ergometer Exercise ◽  
Exercise In Humans

Arterial pH, PCO2, standard bicarbonate, lactate, and ventilation were measured with a high sampling density during rest, exercise, and recovery in normal subjects performing upright cycle ergometer exercise. Three 6-min constant-work exercise tests (moderate, heavy, and very heavy) were performed by each subject. We found a small respiratory acidosis during the moderate-intensity exercise and an early respiratory acidosis followed by a metabolic acidosis for the heavy- and very-heavy-intensity exercise. During recovery, arterial pH rapidly returned to the preexercise value for the moderate-intensity work. However, arterial pH decreased further during the first 2 min of recovery for the heavy- and very-heavy-intensity work, before a slower return toward the resting values. We conclude that arterial acidosis is the consistent arterial pH reaction for moderate-, heavy-, and very-heavy-intensity cycle ergometer exercise in humans and that this acidosis is blunted but not eliminated by the ventilatory response. During recovery, the return to resting arterial pH and PCO2 and standard bicarbonate appears to be determined by the rate of lactate decline.

scholarly journals Cardiopulmonary exercise tests of adolescent elite sport climbers – a comparison of the German junior national team in sport climbing and Nordic skiing

2021 ◽  
Vol 14 (1) ◽  
pp. 1-8
Author(s):  
Isabelle Schöffl ◽  
Jan Wüstenfeld ◽  
Gareth Jones ◽  
Sven Dittrich ◽  
Chris Lutter ◽  
...  
Keyword(s):  
Exercise Test ◽  
Work Load ◽  
Cardiopulmonary Exercise Test ◽  
Cycle Ergometer ◽  
Exercise Tests ◽  
Endurance Sport ◽  
National Team ◽  
Cardiopulmonary Exercise ◽  
Sport Climbing ◽  
Nordic Skiing

Introduction: All the research investigating the cardiopulmonary capacity in climbers is focused on predictors for climbing performance. The effects of climbing on the cardiovascular system in adolescents climbing at an elite level (national team) have not been evaluated.Material and methods: Retrospective analysis of the cardiopulmonary exercise test (CPET) performed on a cycle ergometer during the annual medical examination of the entire German Junior National climbing team on one occasion and for a selected subgroup on two occasions spaced two years apart. The data from the subgroup was compared to an age- and gender-matched control of Nordic skiers from the German Junior National Nordic skiing team. Results: 47 climbers (20 girls, 27 boys) were examined once. The VO2peak achieved by the athletes was 41.3 mL kg<sup>−1</sup> min<sup>−1</sup> (boys) and 39.8 mL kg<sup>−1</sup>min<sup>−1</sup> (girls). 8 boys and 6 girls were tested twice over a period of 27.5 months. The parameters of the exercise test measured on both occasions were significantly lower than those of the 8 male and 6 female Nordic skiers. There was no change with respect to any variables (e.g. VO<sub>2peak</sub>, peak work load, peak heart rate, peak lactate or O<sub>2</sub> pulse) over the examined period. Conclusions: The elite climbers investigated in this study showed comparable VO<sub>2peak</sub> values to athletes from team and combat sports. The Nordic skiers to which they were compared showed significantly higher values consistant with the fact that this is an endurance sport. Even though the cardiopulmonary measurements of the Nordic skiers still improved after two years of training, no adaptations could be observed in the elite climbers.

Mediation of reduced ventilatory response to exercise after endurance training

1987 ◽  
Vol 63 (4) ◽  
pp. 1533-1538 ◽  
Author(s):  
R. Casaburi ◽  
T. W. Storer ◽  
K. Wasserman
Keyword(s):  
Endurance Training ◽  
Venous Blood ◽  
Normal Subjects ◽  
Cycle Ergometer ◽  
Work Rate ◽  
Exercise Tests ◽  
Average Decrease ◽  
Cycle Ergometer Exercise ◽  
Ergometer Exercise ◽  
The Difference

To investigate the mechanism by which ventilatory (VE) demand is modulated by endurance training, 10 normal subjects performed cycle ergometer exercise of 15 min duration at each of four constant work rates. These work rates represented 90% of the anaerobic threshold (AT) work rate and 25, 50, and 75% of the difference between maximum O2 consumption and AT work rates for that subject (as determined from previous incremental exercise tests). Subjects then underwent 8 wk of strenuous cycle ergometer exercise for 45 min/day. They then repeated the four constant work rate tests at work rates identical to those used before training. During tests before and after training, VE and gas exchange were measured breath by breath and rectal temperature (Tre) was measured continuously. A venous blood sample was drawn at the end of each test and assayed for lactate (La), epinephrine (EPI), and norepinephrine (NE). We found that the VE for below AT work was reduced minimally by training (averaging 3 l/min). For the above AT tests, however, training reduced VE markedly, by an average of 7, 23, and 37 l/min for progressively higher work rates. End-exercise La, NE, EPI, and Tre were all lower for identical work rates after training. Importantly, the magnitude of the reduction in VE was well correlated with the reduction in end-exercise La (r = 0.69) with an average decrease of 5.8 l/min of VE per milliequivalent per liter decrease in La. Correlations of VE with NE, EPI, and Tre were much less strong (r = 0.49, 0.43, and 0.15, respectively).

Influence of prior ventilatory experience on the estimation of breathlessness during exercise

1990 ◽  
Vol 78 (2) ◽  
pp. 149-153 ◽  
Author(s):  
Rachel C. Wilson ◽  
P. W. Jones
Keyword(s):  
Exercise Test ◽  
Prior Experience ◽  
Minute Ventilation ◽  
Normal Subjects ◽  
Resistive Load ◽  
Inspiratory Time ◽  
Borg Scale ◽  
Exercise Tests ◽  
Loaded Breathing ◽  
Inspiratory Load

1. The intensity of breathlessness was measured during exercise in nine normal subjects using a modified Borg scale to examine the effect of prior experience of breathlessness on subsequent estimates of breathlessness. 2. Each subject performed four exercise tests, each of which consisted of two identical runs of workload incrementation (run 1 and run 2). An inspiratory resistive load of 3.8 cmH2O s−1 l−1 was applied during the appropriate run of the exercise test to examine the effect of (a) prior experience of ‘loaded’ breathing on breathlessness estimation during ‘unloaded’ breathing, and (b) prior experience of ‘unloaded’ breathing on breathlessness estimation during ‘loaded’ breathing. Run 1 was the conditioning run; run 2 was the run in which the effect of conditioning was measured. 3. There was a good correlation between breathlessness and minute ventilation during both unloaded’ breathing (median r = 0.93) and ‘loaded’ breathing (median r = 0.95). 4. The slope of the Borg score/minute ventilation relationship was greater during ‘loaded’ breathing than during ‘unloaded’ breathing (P < 0.01). There was no difference in mean Borg score between ‘unloaded’ and ‘loaded’ breathing. 5. After a period of ‘loaded’ breathing during run 1, estimated breathlessness was significantly reduced during ensuing ‘unloaded’ breathing in run 2 (P < 0.01) compared with the exercise test in which ‘unloaded’ breathing was experienced throughout both run 1 and run 2. 6. After a period of ‘unloaded’ breathing in run 1, estimated breathlessness was significantly increased during ensuing ‘loaded’ breathing in run 2 (P < 0.01) compared with the exercise test in which the inspiratory load had already been experienced in run 1. 7. Changes in the pattern of breathing (inspiratory time, expiratory time, total breath duration, inspiration time/total breath duration ratio and tidal volume) were not consistent with the changes in breathlessness. 8. We suggest that perception of breathlessness may be influenced by a subject's immediate prior experience of an altered relationship between breathlessness and ventilation.

Maximal oxygen uptake, ventilatory threshold and fat oxidation after an incremental exercise test in triathletes

2017 ◽  
Vol 176 (12) ◽  
Author(s):  
Hanapi M. Johari ◽  
Brinnell A. Caszo ◽  
Victor F. Knight ◽  
Steven A. Lumley ◽  
Aminuddin K. Abdul Hamid ◽  
...  
Keyword(s):  
Oxygen Uptake ◽  
Exercise Test ◽  
Maximal Oxygen Uptake ◽  
Ventilatory Threshold ◽  
Fat Oxidation ◽  
Incremental Exercise ◽  
Incremental Exercise Test

Changes in physiological, haematological and biochemical parameters in police working dogs during a treadmill incremental exercise test

2020 ◽  
Vol 16 (5) ◽  
pp. 387-394
Author(s):  
J.C. Alves ◽  
A. Santos ◽  
P. Jorge ◽  
M.P. Lafuente
Keyword(s):  
Exercise Test ◽  
Biochemical Parameters ◽  
Complete Blood Count ◽  
Rest Period ◽  
Incremental Exercise ◽  
Total Plasma ◽  
Incremental Exercise Test ◽  
Total Plasma Protein ◽  
Working Dogs ◽  
Stage T1

This study aimed to evaluate the physiological, haematological and biochemical changes during a treadmill incremental exercise test (IET). Animals were submitted to five stages of 6 min each, at 6, 7, 8, 9 and 10 mph, at an inclination of 5%. Blood samples were collected at rest (T0), immediately after exercise (T5) and after a 20 min rest period (T6), to determine complete blood count, urea, creatinine, creatine kinase, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, total plasma protein, albumin, alkaline phosphatase (AP), cholesterol, triglycerides (Trig), Ca2+, Na+, K+ and Cl-. Blood lactate (BL), heart rate (HR), rectal temperature (RT) and glycaemia were measured at rest (T0), after each stage (T1-T5) and after the rest period (T6). Variations were recorded between T0 and T5 in red blood cells, haemoglobin, AP, Na+, K+ (P<0.01), Trig (P<0.05), Ca2+ and Cl- (P<0.02). Differences were observed in BL at T5 (P<0.02) and T6 (P<0.02), RT at T2-T6 (P<0.01), HR at T3-T5 (P<0.01) and glycaemia at T2-T4 (P<0.01) and T5 (P<0.05). This study is a novel description of the shifts of physical fit police working dogs during this IET protocol.

scholarly journals Ventilatory compensation during the incremental exercise test is inversely correlated with air trapping in COPD

F1000Research ◽  
2020 ◽  
Vol 8 ◽  
pp. 1661
Author(s):  
Rottem Kuint ◽  
Neville Berkman ◽  
Samir Nusair
Keyword(s):  
Gas Exchange ◽  
Exercise Testing ◽  
Exercise Test ◽  
Cardiopulmonary Exercise Testing ◽  
Incremental Exercise ◽  
Peak Exercise ◽  
Air Trapping ◽  
Incremental Exercise Test ◽  
Cardiopulmonary Exercise ◽  
Copd Patients

Background: Air trapping and gas exchange abnormalities are major causes of exercise limitation in chronic obstructive pulmonary disease (COPD). During incremental cardiopulmonary exercise testing, actual nadir values of ventilatory equivalents for carbon dioxide (V E/VCO 2) and oxygen (V E/VO 2) may be difficult to identify in COPD patients because of limited ventilatory compensation capacity. Therefore, we aimed in this exploratory study to detect a possible correlation between the magnitude of ventilation augmentation, as manifested by increments in ventilatory equivalents from nadir to peak exercise values and air trapping, detected with static testing.    Methods: In this observational study, we studied data obtained previously from 20 COPD patients who, during routine follow-up, underwent a symptom-limited incremental exercise test and in whom a plethysmography was obtained concurrently. Air trapping at rest was assessed by measurement of the residual volume (RV) to total lung capacity (TLC) ratio (RV/TLC). Gas exchange data collected during the symptom-limited incremental cardiopulmonary exercise test allowed determination of the nadir and peak exercise values of V E/VCO 2 and V E/VO 2, thus enabling calculation of the difference between peak exrcise value and nadir values of  V E/VCO 2 and V E/VO 2, designated ΔV E/VCO 2 and ΔV E/VO 2, respectively. Results: We found a statistically significant inverse correlation between both ΔV E/VCO 2 (r = -0. 5058, 95% CI -0.7750 to -0.08149, p = 0.0234) and ΔV E/VO 2 (r = -0.5588, 95% CI -0.8029 to -0.1545, p = 0.0104) and the degree of air trapping (RV/TLC). There was no correlation between ΔV E/VCO 2 and forced expiratory volume in the first second, or body mass index.  Conclusions: The ventilatory equivalents increment to compensate for acidosis during incremental exercise testing was inversely correlated with air trapping (RV/TLC).

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