Effect of hypoxia on gene expression by human hepatocytes (HepG2)

2003 ◽  
Vol 12 (3) ◽  
pp. 195-207 ◽  
Author(s):  
Larry A. Sonna ◽  
Michael L. Cullivan ◽  
Holly K. Sheldon ◽  
Richard E. Pratt ◽  
Craig M. Lilly
Keyword(s):  
Gene Expression ◽  
Hepg2 Cells ◽  
Cdna Array ◽  
Hypoxic Stress ◽  
Control Room ◽  
Significant Component ◽  
Oligonucleotide Arrays ◽  
Number Of Genes ◽  
Late Generation ◽  
Induced Changes

The full extent to which hypoxia produces gene expression changes in human cells is unknown. We used late-generation oligonucleotide arrays to catalog hypoxia-induced changes in gene expression in HepG2 cells. Five paired sets of cultures were subjected to either control (room air-5% CO2) or hypoxic (1% O2-5% CO2) conditions for 24 h, and RNA was analyzed on an Affymetrix cDNA array containing ∼12,600 sequences. A statistically significant change in expression was shown by 2,908 sequences (1,255 increased and 1,653 decreased). The observed changes were highly concordant with published literature on hypoxic stress but showed relatively little overlap (12–22%) with changes in gene expression that have been reported to occur after heat stress in other systems. Of note, of these 2,908 sequences, only 387 (213 increased and 174 decreased) both exhibited changes in expression of twofold or greater and were highly expressed in at least three of the five experiments. We conclude that the effect of hypoxia on gene expression by HepG2 cells is broad, has a significant component of downregulation, and includes a relatively small number of genes whose response is truly independent of cell and stress type.

Temporal profiling of methamphetamine-induced changes in gene expression in the mouse brain: Evidence from cDNA array

Synapse ◽  
2001 ◽  
Vol 41 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Jean Lud Cadet ◽  
Subramaniam Jayanthi ◽  
Michael T. Mccoy ◽  
Marquis Vawter ◽  
Bruce Ladenheim
Keyword(s):  
Gene Expression ◽  
Mouse Brain ◽  
Cdna Array ◽  
Induced Changes

scholarly journals Single-Cell Transcript Profiling of Barley Attacked by the Powdery Mildew Fungus

2007 ◽  
Vol 20 (3) ◽  
pp. 235-246 ◽  
Author(s):  
Torben Gjetting ◽  
Peter H. Hagedorn ◽  
Patrick Schweizer ◽  
Hans Thordal-Christensen ◽  
Timothy L. W. Carver ◽  
...  
Keyword(s):  
Gene Expression ◽  
Powdery Mildew ◽  
Expression Profiles ◽  
Cdna Array ◽  
Gene Expression Profiles ◽  
Powdery Mildew Fungus ◽  
Nonspecific Resistance ◽  
Susceptible Host ◽  
Leaf Epidermal Cell ◽  
Induced Changes

In many plant-pathogen interactions, there are several possible outcomes for simultaneous attacks on the same leaf. For instance, an attack by the powdery mildew fungus on one barley leaf epidermal cell may succeed in infection and formation of a functional haustorium, whereas a neighboring cell attacked at the same time may resist fungal penetration. To date, the mixed cellular responses seen even in susceptible host leaves have made it difficult to relate induced changes in gene expression to resistance or susceptibility in bulk leaf samples. By microextraction of cell-specific mRNA and subsequent cDNA array analysis, we have successfully obtained separate gene expression profiles for specific mildew-resistant and -infected barley cells. Thus, for the first time, it is possible to identify genes that are specifically regulated in infected cells and, presumably, involved in fungal establishment. Further, although much is understood about the genetic basis of effective papilla resistance associated with mutant mlo barley, we provide here the first evidence for gene regulation associated with effective papilla-based nonspecific resistance expressed in nominally “susceptible” wild-type barley.

scholarly journals Methamphetamine-induced changes in myocardial gene transcription are sex-dependent

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hasitha Chavva ◽  
Daniel A. Brazeau ◽  
James Denvir ◽  
Donald A. Primerano ◽  
Jun Fan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ischemic Injury ◽  
Female Rats ◽  
Dependent Effect ◽  
Methamphetamine Use ◽  
Number Of Genes ◽  
Myocardial Ischemic Injury ◽  
Induced Changes ◽  
Myocardial Gene Expression

Abstract Background Prior work demonstrated that female rats (but not their male littermates) exposed to methamphetamine become hypersensitive to myocardial ischemic injury. Importantly, this sex-dependent effect persists following 30 days of subsequent abstinence from the drug, suggesting that it may be mediated by long term changes in gene expression that are not rapidly reversed following discontinuation of methamphetamine use. The goal of the present study was to determine whether methamphetamine induces sex-dependent changes in myocardial gene expression and whether these changes persist following subsequent abstinence from methamphetamine. Results Methamphetamine induced changes in the myocardial transcriptome were significantly greater in female hearts than male hearts both in terms of the number of genes affected and the magnitude of the changes. The largest changes in female hearts involved genes that regulate the circadian clock (Dbp, Per3, Per2, BMal1, and Npas2) which are known to impact myocardial ischemic injury. These genes were unaffected by methamphetamine in male hearts. All changes in gene expression identified at day 11 returned to baseline by day 30. Conclusions These data demonstrate that female rats are more sensitive than males to methamphetamine-induced changes in the myocardial transcriptome and that methamphetamine does not induce changes in myocardial transcription that persist long term after exposure to the drug has been discontinued.

scholarly journals Analysis of isoproterenol-induced changes in parotid gland gene expression

2002 ◽  
Vol 8 (2) ◽  
pp. 107-114 ◽  
Author(s):  
Kelly G. Ten Hagen ◽  
Marlene M. Balys ◽  
Lawrence A. Tabak ◽  
James E. Melvin
Keyword(s):  
Gene Expression ◽  
Parotid Gland ◽  
Expression Patterns ◽  
Systemic Exposure ◽  
Oligonucleotide Array ◽  
Oligonucleotide Arrays ◽  
Starting Point ◽  
Array Technology ◽  
Induced Changes ◽  
Expressed Sequence

Parotid gland acinar cells undergo marked hypertrophy and hyperplasia upon systemic exposure to the β-adrenergic agonist, isoproterenol. This glandular enlargement is accompanied by substantial cellular changes including DNA synthesis, an increase in glandular protein synthesis, and differential changes in RNA transcription. To gain a more detailed understanding of the underlying changes induced by isoproterenol, we have examined the parotid gland gene expression profile of mice up to 24 h post-isoproterenol injection using high-density oligonucleotide arrays. Depending upon the exposure time, between 22 and 48 of the ∼6,500 mouse genes and expressed sequence tags (ESTs) analyzed displayed significant changes in expression patterns. Genes that were previously shown to be repressed (α-amylase) or activated (proline-rich proteins) following isoproterenol exposure were found to be similarly affected in this experiment, validating this technique. This study demonstrates that the oligonucleotide array technology is a useful tool for examining isoproterenol-induced salivary gland gene expression changes. Using this as a starting point, we can begin to dissect the specific pathways involved in mediating isoproterenol action within the parotid gland.

Microarray reveals positive effects of green and black tea polyphenols on TNF[alpha]-induced changes of gene expression

2013 ◽  
Author(s):  
Husna Zulkipli ◽  
Norita Salim ◽  
Gabriele Anisah Froemming ◽  
Aletza Mohd Ismail ◽  
Hapizah Nawawi
Keyword(s):  
Gene Expression ◽  
Black Tea ◽  
Tea Polyphenols ◽  
Tnf Alpha ◽  
Positive Effects ◽  
Black Tea Polyphenols ◽  
Green And Black Tea ◽  
Induced Changes

Cell-specific regulation of IRS-1 gene expression: role of E box and C/EBP binding site in HepG2 cells and CHO cells

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 354-362 ◽  
Author(s):  
K. Matsuda ◽  
E. Araki ◽  
R. Yoshimura ◽  
K. Tsuruzoe ◽  
N. Furukawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Binding Site ◽  
Cho Cells ◽  
Hepg2 Cells ◽  
E Box ◽  
Specific Regulation

Hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in HepG2 cells

2020 ◽  
Vol 01 ◽  
Author(s):  
Ayşe Mine Yılmaz ◽  
Gökhan Biçim ◽  
Kübra Toprak ◽  
Betül Karademir Yılmaz ◽  
Irina Milisav ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Cell Viability ◽  
Hepg2 Cells ◽  
Proteasome Activity ◽  
Cell Cycle Phases ◽  
Induced Changes ◽  
And Oxidative Stress ◽  
Quercetin Treatment

Background: Different cellular responses influence the progress of cancer. In this study, we have investigated the effect of hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in human hepatocellular carcinoma (HepG2) cells. Methods: The effects of hydrogen peroxide and quercetin on cell viability, cell cycle phases and oxidative stress related cellular changes were investigated. Cell viability was assessed by WST-1 assay. Apoptosis rate, cell cycle phase changes and oxidative stress were measured by flow cytometry. Protein expressions of p21, p27, p53, NF-Kβ-p50 and proteasome activity were determined by Western blot and fluorometry, respectively. Results: Hydrogen peroxide and quercetin treatment resulted in decreased cell viability and increased apoptosis in HepG2 cells. Proteasome activity was increased by hydrogen peroxide but decreased by quercetin treatment. Conclusion: Both agents resulted in decreased p53 protein expression and increased cell death by different mechanisms regarding proteostasis and cell cycle phases.

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