scholarly journals Thrombopoietin Receptor Levels in Tumor Cell Lines and Primary Tumors

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Connie L. Erickson-Miller ◽  
Antony Chadderton ◽  
Anna Gibbard ◽  
Jennifer Kirchner ◽  
Kodandaram Pillarisetti ◽  
...  
Keyword(s):  
Growth Factor ◽  
Tumor Cell ◽  
Cell Lines ◽  
Hematologic Malignancies ◽  
Erythropoietin Receptor ◽  
Tumor Cell Lines ◽  
Hepatitis C Infection ◽  
Primary Tumors ◽  
Normal Tissues ◽  
Proliferation And Differentiation

Thrombopoietin (TPO) receptor agonists represent a new approach for the treatment of thrombocytopenia, which may develop as a consequence of immune thrombocytopenia, chemotherapy treatment, chronic hepatitis C infection, or myelodysplastic syndromes. There are concerns that use of certain growth factors can hasten disease progression in some types of hematologic malignancies and solid tumors. In this study, expression ofMPL(TPO-R) mRNA was examined in tumor cell lines, patient tumor samples (renal cell carcinoma, prostatic carcinoma, soft tissue and bony/cartilage sarcoma, colon cancer, and lymphoma), and normal tissues using microarray analysis and qRT-PCR.MPLmRNA is expressed at very low or undetectable levels compared with erythropoietin receptor (EPOR), human epidermal growth factor (ERBB2; HER2), and insulin-like growth factor-1 receptor (IGF1R) in these patient samples. These data suggest TPO-R agonists will likely preferentially stimulate proliferation and differentiation of cells of megakaryocytic lineage, potentially demonstrating their utility for correcting thrombocytopenia in clinical settings.

scholarly journals Characteristics of specific binding of epidermal growth factor (EGF) on human tumor cell lines.

1983 ◽  
Vol 30 (5) ◽  
pp. 601-607 ◽  
Author(s):  
YUKIO HIRATA ◽  
MASAHITO UCHIHASHI ◽  
TAKUO FUJITA ◽  
SHIGERU MATSUKURA ◽  
TEIICHI MOTOYAMA ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Tumor Cell ◽  
Cell Lines ◽  
Specific Binding ◽  
Human Tumor ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
Epidermal Growth

scholarly journals High periostin expression correlates with aggressiveness in papillary thyroid carcinomas

2008 ◽  
Vol 197 (2) ◽  
pp. 401-408 ◽  
Author(s):  
Cinzia Puppin ◽  
Dora Fabbro ◽  
Mariavittoria Dima ◽  
Carla Di Loreto ◽  
Efisio Puxeddu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Thyroid Tumor ◽  
Specific Gene ◽  
Mrna Levels ◽  
Tumor Cell Lines ◽  
Papillary Thyroid ◽  
Osteoblastic Cell Line ◽  
Thyroid Carcinomas ◽  
Normal Tissues

Periostin is a mesenchyme-specific gene product, which acts as an adhesion molecule during bone formation and supports osteoblastic cell line attachment and spreading. However, periostin expression is activated in a large variety of epithelial human tumors and correlates with their aggressiveness. Knowledge of expression of periostin in thyroid tumors is still scanty. The aim of the present work was to investigate periostin expression in differentiated neoplasms of the thyroid and to correlate it with several clinical and molecular features of these tumors. Periostin expression was evaluated by quantitative PCR and immunohistochemistry in normal thyroid tissues, papillary thyroid carcinomas (PTCs), follicular thyroid carcinomas (FTCs), and follicular adenomas (FAs). Periostin mRNA levels were also evaluated in several thyroid tumor cell lines. PTCs show mean periostin mRNA levels significantly higher than corresponding normal tissues. In five PTCs, periostin mRNA values were at least 30-fold higher than corresponding normal tissues. Conversely, mean periostin mRNA levels of FTCs and FAs were similar to those of normal tissues. Consistent with mRNA studies, periostin was detectable by immunohistochemistry in cancerous epithelial cells only in several cases of PTCs but not in normal tissue, FTCs, and FAs. In PTCs, periostin mRNA levels positively correlate with extrathyroidal invasion, distant metastasis, and higher grade staging. A negative correlation between periostin and expression of some markers of the thyroid-differentiated phenotype (thyroglobulin, thyrotropin receptor) was also present in the PTCs. These results indicate that an increase in periostin gene expression is present in several PTCs, in which it appears as a marker of aggressiveness. Experiments in thyroid tumor cell lines indicate that high levels of periostin mRNA are due, at least in part, to the increase in periostin promoter activity.

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