scholarly journals Effect of Ecotype and Latitude on Growth, Frost Hardiness, and Oxidative Stress of South to North Transplanted Scots Pine Seedlings

2010 ◽  
Vol 2010 ◽  
pp. 1-16 ◽  
Author(s):  
Erja Taulavuori ◽  
Kari Taulavuori ◽  
Ahti Niinimaa ◽  
Kari Laine
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Total Nitrogen ◽  
Scots Pine ◽  
Protein Oxidation ◽  
Reductase Activity ◽  
Shoot Elongation ◽  
Frost Hardiness ◽  
Glutathione Reductase Activity ◽  
And Oxidative Stress

Ecotypes of Pinus sylvestris seedlings from Kuhmo (64°N) and Ranua (66°N) were transplanted to 0 (control), 1, 2, and 3°N higher latitude in Northern Finland in 1997. Sampling was carried out twice per year (spring/autumn) during 1998–2000. Shoot elongation, total nitrogen concentration, frost hardiness and oxidative stress state (lipid peroxidation, glutathione reductase activity, and protein oxidation) in the needles were analyzed. Comparison between the seasonal sampling times indicated that total nitrogen, lipid peroxidation, glutathione reductase activity, and protein oxidation were lower in autumn, during which the frost hardiness was higher. The above suggests that the stress conditions were higher in spring. Comparison between the origins of the seedlings demonstrated that shoot elongation of the northern origin were minor, while their total nitrogen concentrations and frost hardiness were more pronounced. Effect of latitude on elongation of Scots pine is most probably a result of a combination of temperature and light quality. No delay in frost hardening due to higher latitude was observed during the autumnal equinox. No marked stress caused by transplantation to the north was either observed. It is concluded that southern ecotypes of Scots pine have the potential to adapt to migration driven by a warming climate.

Proteasome activity in experimental diabetes

2006 ◽  
Vol 1 (2) ◽  
pp. 289-298 ◽  
Author(s):  
Albena Alexandrova ◽  
Lubomir Petrov ◽  
Margarita Kirkova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Oxidation ◽  
Experimental Diabetes ◽  
Diabetic Rats ◽  
Ubiquitin Proteasome Pathway ◽  
Ubiquitin Proteasome ◽  
Proteasome Pathway ◽  
And Oxidative Stress

AbstractNumerous studies have indicated that oxidative stress contributes to the development and progression of diabetes and other related complications. Since the ubiquitin-proteasome pathway is involved in degradation of oxidized proteins, it is to be expected that alterations in proteasome-dependent proteolysis accompany diabetes. This paper focuses on the role of the proteasome in alloxan-induced experimental diabetes. The changes in proteasomal activity and oxidative stress indices (protein oxidation and lipid peroxidation) were evaluated. The obtained results revealed increased protein oxidation and lipid peroxidation, as well as alterations in proteasomal activities in diabetic rats. Our data indicates a significant decrease in chymotryptic-like activity; increased tryptic-like activity; and unchanged post-glutamyl peptide hydrolytic-like activity. These findings suggest the presence of oxidative stress in diabetes that appears to result in changes to the ubiquitin-proteasome pathway.

scholarly journals The relationship between vitamin D deficiency and oxidative stress can be independent of age and gender

2019 ◽  
pp. 1-16 ◽  
Author(s):  
Alice Barros Câmara ◽  
Igor Augusto Brandão
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Protein Oxidation ◽  
Age And Gender ◽  
Gender And Age ◽  
And Gender ◽  
The Relationship ◽  
And Oxidative Stress

Abstract. The active vitamin D (1,25(OH)2D) acts through a nuclear receptor to perform several functions in cellular metabolism. 1,25(OH)2D participates directly in calcium homeostasis, regulates the immune system, nervous system, blood pressure, insulin secretion, among others. Vitamin D deficiency could also be associated with several diseases and increased cellular oxidative damage. The present study aimed to investigate whether lipid peroxidation and/or protein oxidation are affected by vitamin D deficiency and whether sunlight exposure/diet, gender, and age might influence this relationship. Vitamin D concentrations were obtained from the Heart Hospital database and a questionnaire was applied among the 212 participants. We used the inactive vitamin D (25(OH)2) in the analyses since 1,25(OH)2D has a short half-life and a low blood concentration. Lipid peroxidation and protein oxidation analyses were performed using spectrophotometry. Multivariate analyses suggested the participation of vitamin D deficiency (<30 ng/mL) and sunlight/diet in oxidative stress (p <0.05; R2 MDA: 0.562; R2 CG: 0.429). Multiple linear regression test show that the age and gender of patients are not interfering in the analyses (p>0.05). Therefore, we suggest that the relationship between vitamin D deficiency and oxidative stress can be independent of age and gender.

Nose-only water-pipe smoking effects on airway resistance, inflammation, and oxidative stress in mice

2013 ◽  
Vol 115 (9) ◽  
pp. 1316-1323 ◽  
Author(s):  
Abderrahim Nemmar ◽  
Haider Raza ◽  
Priya Yuvaraju ◽  
Sumaya Beegam ◽  
Annie John ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Airway Resistance ◽  
Reduced Glutathione ◽  
Mechanistic Explanation ◽  
The United States ◽  
Forced Oscillation Technique ◽  
Water Pipe ◽  
Respiratory Effects ◽  
And Oxidative Stress

Water-pipe smoking (WPS) is a common practice in the Middle East and is now gaining popularity in Europe and the United States. However, there is a limited number of studies on the respiratory effects of WPS. More specifically, the underlying pulmonary pathophysiological mechanisms related to WPS exposure are not understood. Presently, we assessed the respiratory effects of nose-only exposure to mainstream WPS generated by commercially available honey flavored “moasel ” tobacco. The duration of the session was 30 min/day and 5 days/wk for 1 mo. Control mice were exposed to air only. Here, we measured in BALB/c mice the airway resistance using forced-oscillation technique. Lung inflammation was assessed histopathologically and by biochemical analysis of bronchoalveolar lavage (BAL) fluid, and oxidative stress was evaluated biochemically by measuring lipid peroxidation, reduced glutathione and several antioxidant enzymes. Pulmonary inflammation assessment showed an increase in neutrophil and lymphocyte numbers. Likewise, airway resistance was significantly increased in the WPS group compared with controls. Tumor necrosis factor α and interleukin 6 concentrations were significantly increased in BAL fluid. Lipid peroxidation in lung tissue was significantly increased whereas the level and activity of antioxidants including reduced glutathione, glutathione S transferase, and superoxide dismutase were all significantly decreased following WPS exposure, indicating the occurrence of oxidative stress. Moreover, carboxyhemoglobin levels were significantly increased in the WPS group. We conclude that 1-mo nose-only exposure to WPS significantly increased airway resistance, inflammation, and oxidative stress. Our results provide a mechanistic explanation for the limited clinical studies that reported the detrimental respiratory effects of WPS.

scholarly journals Strawberry Intake Ameliorates Oxidative Stress and Decreases GABA Levels Induced by High-Fat Diet in Frontal Cortex of Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 70 ◽  
Author(s):  
Cuauhtémoc Sandoval-Salazar ◽  
Cecilia Oviedo-Solís ◽  
Edmundo Lozoya-Gloria ◽  
Herlinda Aguilar-Zavala ◽  
Martha Solís-Ortiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Frontal Cortex ◽  
Protein Oxidation ◽  
High Fat Diet ◽  
High Fat ◽  
Gaba Concentration ◽  
Obese Rats ◽  
Lipid And Protein Oxidation

It has been proposed that there is a correlation between high-fat diet (HFD), oxidative stress and decreased γ-aminobutyric acid (GABA) levels, but this has not been thoroughly demonstrated. In the present study, we determined the effects of strawberry extract intake on the oxidative stress and GABA levels in the frontal cortex (FC) of obese rats. We observed that an HFD increased lipid and protein oxidation, and decreased GABA levels. Moreover, UV-irradiated strawberry extract (UViSE) decreased lipid peroxidation but not protein oxidation, whereas non-irradiated strawberry extract (NSE) reduced protein oxidation but not lipid peroxidation. Interestingly, NSE increased GABA concentration, whereas UViSE was not as effective. In conclusion, our results suggest that an HFD increases oxidative damage in the FC, whereas strawberry extract intake may ameliorate the disturbances associated with HFD-induced oxidative damage.

scholarly journals Oxidative Stress Induction by Lead in Leaves of Radish (Raphanus sativus) Seedlings

2011 ◽  
Vol 3 (4) ◽  
pp. 93-99 ◽  
Author(s):  
Nadjet BITEUR ◽  
Abdelkader AOUES ◽  
Omar KHAROUBI ◽  
Miloud SLIMANI
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Oxidation ◽  
Raphanus Sativus ◽  
Lead Concentration ◽  
Control Treatment ◽  
Enzymatic Antioxidants ◽  
Carotenoid Concentration ◽  
Hydroponic System ◽  
Physiological Processes

Oxidative stress was induced by lead acetate (Pb) in Raphanus sativus seedlings grown in a hydroponic system using sand as substrate. Thirty day old acclimated seeds were treated for 7 days with five Pb levels (0 as control, 100, 200, 500 and 1000 mg l-1). Parameters such as growth, oxidative damage markers (lipid peroxidation, protein oxidation and hydrogen peroxide contents) and enzymatic activities of catalase (CAT) and peroxidase (POD) were investigated. Lead concentration in plant tissues increased with increasing of Pb levels. Shoot fresh weight, chlorophyll and carotenoid concentration were significantly decreased at 100 mg l-1 Pb. Lipid peroxidation, protein oxidation and H2O2 levels were increased at 500 and 1000 mg l-1 Pb compared to control treatment, in shoots. Peroxidase activity showed a straight correlation with H2O2 concentration, whereas CAT activity decreased only in shoots. These changes in enzymatic and non-enzymatic antioxidants showed that the Pb exposition had a significant disturbance on Raphanus sativus plantlets and affect the biochemical and physiological processes.

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