scholarly journals In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz

2011 ◽  
Vol 8 (3) ◽  
pp. 1451-1455
Author(s):  
Ramalingam Mahesh ◽  
Hyo Won Jung ◽  
Jun Hong Park ◽  
Yong-Ki Park
Keyword(s):  
Cell Viability ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Neuronal Cell ◽  
Ethyl Acetate Fraction ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Ostericum Koreanum

Ostericum koreanummaximowicz (Umbelliferae), a medicinal herb in Korean Oriental Medicine, has been applied to treat cold, headache, neuralgia and arthralgia. The ethyl acetate fraction ofO. koreanumroot was subjected toin vitroantioxidant activity with different methods for free radical scavenging activities. In addition, the cell viability and nitric oxide release assays were performed here for the first time in neuroblastoma (Neuro-2a) cell cultures. Among all the tested methods, the ethyl acetate fraction was expressed very active, exhibiting a good Trolox equivalent values and IC50, comparable to that of the commercial antioxidants, Trolox and ascorbic acid, respectively. The results showed that there was a reduction of cell viability by the fraction in a concentration dependent manner. These results suggest thatO. koreanumshows good antioxidant activitiesin vitroby inhibiting free radicals. These findings provide a rationale for thein vivotesting. Also, the major constituents behind the antioxidant mechanisms of this fraction warrant further study.

scholarly journals Antioxidant Activities of Extracts from Sarcocarp of Cotoneaster multiflorus

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Xiangping Liu ◽  
Jia Jia ◽  
Xuemin Jing ◽  
Guoliang Li
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Ethyl Acetate Fraction ◽  
Dependent Manner ◽  
Antioxidant Power ◽  
Water Fraction

The ethanol-water (7 : 3, v/v) extract of Cotoneaster multiflorus sarcocarp was sequentially fractionated by liquid-liquid partition using n-hexane, diethyl ether, methylene dichloride, and ethyl acetate. The contents of total polyphenols, total flavones, and oligomeric proanthocyanidins in the five parts (including the ethanol-water extract) were determined. In addition, 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation decolorization, reducing power, ferric reducing antioxidant power, and lipid peroxidation inhibition assays were conducted to test the antioxidant activities of Sample 1 (the ethanol-water fraction) and Sample 2 (the ethyl acetate fraction) in vitro. In the above five assays, Sample 2 showed greater antioxidant capacities than Sample 1. Furthermore, Sample 2 was better able to protect low-density lipoproteins from oxidation in a dose-dependent manner. The test results show that C. multiflorus sarcocarp, especially the ethyl acetate-soluble fraction, may be a potential source of natural antioxidants.

Antioxidant Activity of Fruits of Perillafrutescens

2014 ◽  
Vol 644-650 ◽  
pp. 5262-5265
Author(s):  
Jing Rong Song ◽  
Gang Lv
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Dpph Method

The antioxidant activities of extracts and residuum of Perillafrutescens fruits from supercritical CO2 extraction were determined in vitro. The residuum was extracted in turn with water, propyl alcohol and ethyl acetate. The antioxidant activities of the extracts were assayed with antioxidant capacity in linoleic acid model system, reducing powers, radical scavenging activity using 1,1-diphenyl-2-picrylhy-drazyl (DPPH) method. The results show that the ethyl acetate extract of Perillafrutescens possesses strongest DPPH radical scavenging activity and reducing power in a concentration-dependent manner.

scholarly journals In Vitro Antiproliferative and Antioxidant Effects of Extracts from Rubus caesius Leaves and Their Quality Evaluation

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Daniel Mirosław Grochowski ◽  
Roman Paduch ◽  
Adrian Wiater ◽  
Adrianna Dudek ◽  
Małgorzata Pleszczyńska ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethyl Acetate Fraction ◽  
Human Colon ◽  
Dependent Manner ◽  
Human Colon Cancer ◽  
Concentration Dependent Manner ◽  
Two Stages

The present study was performed to evaluate the effect of different extracts and subfractions from Rubus caesius leaves on two human colon cancer cell lines obtained from two stages of the disease progression lines HT29 and SW948. Tested samples inhibited the viability of cells, both HT29 and SW948 lines, in a concentration-dependent manner. The most active was the ethyl acetate fraction which, applied at the highest concentration (250 μg/mL), decreased the viability of cells (HT29 and SW948) below 66%. The extracts and subfractions were also investigated for antioxidant activities on DPPH and FRAP assays. All extracts, with the exception of water extract at a dose of 250 μg/mL, almost totally reduced DPPH. The highest Fe3+ ion reduction was shown for the diethyl and ethyl acetate fractions. It was more than 6.5 times higher (at a dose 250 μg/mL) as compared to the control. The LC-MS studies of the analysed preparations showed that all samples contain a wide variety of polyphenolics, among which ellagitannins turned out to be the main constituents with dominant ellagic acid, sanguiin H-6, and flavonol derivatives.

scholarly journals Antioxidant Activities of the Leaf Extract and Fractions of Dryopteris filix-mas (L.) Schott could be Attributed to The Abundance of Polyphenol Compounds

2020 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Earnest Oghenesuvwe Erhirhie ◽  
Emmanuel Emeka Ilodigwe ◽  
Daniel Lotanna Ajaghaku ◽  
Blessing Ogechukwu Umeokoli ◽  
Peter Maduabuchi Eze ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bioactive Compound ◽  
Ethyl Acetate Fraction ◽  
In Vitro Assays ◽  
Anti Oxidant

Dryopteris filix mas (D filix-mas) is wildly used in ethnomedicine for the management of rheumatoid arthritis, wounds and other diseases. We investigated the anti-oxidant activities of its leaf extract, and chromatographic fractions. The ethanol leaf extract was partitioned into four fractions; n-hexane, ethyl acetate, n-butanol and water. Ferric reducing anti-oxidant power (FRAP), 1, 1-diphenyl-2-picrylhydrazil (DPPH) and nitric oxide (NO) scavenging in vitro assays were carried out on the extract and fractions at 6.25, 12.5, 25, 50, 100, 200, 400 and 800 µg/mL. The most active fraction (ethyl acetate fraction) was further purified using chromatographic techniques to isolate its major compound whose structure was elucidated using ID nuclear magnetic resonance (NMR) and mass spectrometry. The ethyl acetate fraction produced the highest free radical scavenging activity among the other fractions. The fraction (VLC-E7) from which the bioactive compound, quercetin-3-O-αL-rhamnopyranoside, was isolated had the best FRAP and DPPH scavenging activities with EC50 and IC50 values of 88.81 ± 3.41 and 26.87 ± 0.24 respectively more than the ethyl acetate fraction. This study revealed that the polyphenol flavonoid, quercetin-3-O-αL-rhamnopyranoside could be responsible for antioxidant activity of ethno-medicinal property of D filix-mas leaf.

scholarly journals Antioxidant and antibacterial activity of extracts, fractions and isolated substances from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don

2010 ◽  
Vol 46 (4) ◽  
pp. 715-722 ◽  
Author(s):  
Cláudia Alexandra de Andrade ◽  
João Luiz de Souza Carvalho ◽  
Miriam Machado Cunico ◽  
Ana Luísa Lacava Lordello ◽  
Carmen Etsuko Kataoka Higaskino ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Thiobarbituric Acid ◽  
Ethyl Acetate Fraction ◽  
Total Phenol Content

The extracts and fractions from the flowers of A. podalyriifolia were analyzed previously for antibacterial activity using diffusion in disk, Antioxidant properties were evaluated by determining radical scavenging power (DPPH test) and total phenol content was measured (Folin method). The present study describes the in vitro antibacterial (determining minimum inhibitory concentration) and antioxidant activities (by thiobarbituric acid reactive species - TBARS method) for the ethanol extract, dichloromethane and ethyl acetate fractions and two flavanones (naringenin and 5-β-D-glycosyl-naringenin) isolated from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don. The flavanones naringenin and 5-β-D-glycosyl-naringenin had not previously been obtained from this species. The most effective antibacterial activity was observed in the ethyl acetate fraction (MIC=0.25 mg mL-1 against Staphylococcus aureus ATCC 6538, MIC = 0.125 mg mL-1 against Staphylococcus epidermidis ATCC 12229, MIC=0.5 mg mL-1 against Streptococcus pyogenes ATCC 19615, Klebsiella pneumoniae ATCC 13883 and Proteus mirabilis ATCC 43071). The evaluated samples showed antioxidant activity on the TBARS test, especially for ethanol extract (1000 ppm), which was the most active (29.43% ± 0.65) followed by ethyl acetate fraction (1000 ppm, 24.84% ± 1,28), both demonstrating higher activity than that presented by ascorbic acid (1000 ppm, 21.73% ± 1.77), although lower than the BHT (1000 ppm 35.15% ± 3.42), both reference compounds. Naringenin and 5-β-D-glycosyl-naringenin demonstrated antioxidant action, but only naringenin inhibited the growth of gram-positive and gram-negative bacteria.

scholarly journals Acetone Extract fromRhodomyrtus tomentosa: A Potent Natural Antioxidant

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Goodla Lavanya ◽  
Supayang Piyawan Voravuthikunchai ◽  
Nongporn Hutadilok Towatana
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Antioxidant Power ◽  
Lipid Peroxidation Inhibition

Rhodomyrtus tomentosa(Myrtaceae) has been employed in traditional Thai medicine to treat colic diarrhoea, dysentery, abscesses, haemorrhage, and gynaecopathy. In addition, it has been used to formulate skin-whitening, anti-aging and skin beautifying agents. Ethnomedical activities of this plant may be due its antioxidant property. Hence, the aim of this study was to evaluate bothin vitroandin vivoantioxidant activities ofR. tomentosaleaf extract.In vitroantioxidant activity of the extract was assessed by lipid peroxidation inhibition capacity, ferric reducing antioxidant power, and metal chelating activity.R. tomentosaextract demonstrated its free radical scavenging effects in concentration dependent manner.In vivoantioxidant activity of the extract was conducted in SwissAlbinomice. Levels of thio-barbituric acid reactive substances (TBARS), glutathione (GSH), and the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in blood, liver, and kidney were analyzed using microtitre plate photometer. Administration of CCl4caused significant increase in TBARS and decrease in GSH, SOD, CAT and GPx levels. In contrast,R. tomentosaextract (0.8 g/kg) effectively prevented these alterations and maintained the antioxidant status. The results suggest thatR. tomentosaextract can serve as a potent antioxidant.

scholarly journals In-vitro and in-vivo Antioxidant Activity of the Butanolic Extract from the Stem of Ephedra alte

2018 ◽  
Vol 11 (3) ◽  
pp. 1239-1245 ◽  
Author(s):  
Bahaa Al-Trad ◽  
Mahmoud A Al –Qudah ◽  
Mazhar Al Zoubi ◽  
Alaa Al-Masri ◽  
Riyadh Muhaidat ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Dependent Manner ◽  
Sod Activity ◽  
Concentration Dependent Manner ◽  
Butanolic Extract ◽  
Phytochemical Components

Previous studies indicated that the extracts from different Ephedra species have antibacterial, antifungal and antioxidant activities. However, none of the published report described the phytochemical components and the antioxidant capacities of Ephedra alte belonging to the family Ephedraceae. To evaluate the in-vitro and in-vivo antioxidant activities of the butanolic extract from stems of Ephedra alte from northern Jordan. Graded concentrations of butanolic extracts from stems of E. alte plant were subjected to four different in-vitro antioxidant assays (DPPH, ABTS, ferrous ion chelating and hydroxyl radical scavenging activities). The in-vivo effects of two different doses of the extract (200 mg/kg and 500 mg/kg, orally for 12 days) on the activities of serum and liver superoxide dismutase (SOD) and catalase (CAT) were measured in mice. Strong in-vitro antioxidant activities in a concentration-dependent manner were recorded. As well, significant increases in both liver and serum CAT enzyme activity and in serum SOD activity were observed in mice treated for 12 days with the extract. These results suggested that the butanolic extract from stems of exhibited significant in-vitro and in-vivo antioxidant activities, supporting the use of E. alte as an important source of natural antioxidants.

scholarly journals Antioxidant Activities and Identification of an Active Compound from Rambutan (Nephelium lappaceum L.) Peel

2020 ◽  
Author(s):  
Mistriyani Mistriyani ◽  
Sugeng Riyanto ◽  
Anjar Windarsih ◽  
Abdul Rohman
Keyword(s):  
Ethyl Acetate ◽  
Active Compound ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Active Compounds ◽  
Beneficial Effects ◽  
Metal Chelating ◽  
Nephelium Lappaceum

The consumption of rambutan fruit resulted in a vast amount of peels and seeds waste. Therefore, the exploration of active compounds having beneficial effects on human health, such as antioxidants, is very lucrative. This research was aimed to isolate and to identify the active compound as an antioxidant from rambutan peel. The powdered rambutan peel was extracted with a maceration technique using methanol then fractionated using petroleum ether, chloroform, and ethyl acetate to get the corresponding fractions. The extract and fractions were determined for its antioxidant activities in vitro using 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and metal-chelating assay. The ethyl acetate fraction exhibited the highest antiradical activity with an IC50 value of 26.22 μg/mL and metal-chelating activity, accounting for 12.32%. The antioxidant activities of extract and fractions correlated with its phenolics and flavonoid contents. Identification of active compounds using FTIR, GC-MS, and NMR resulted in the chemical formula of C7H6O4, identified as 3,4-dihydroxybenzoic acid.

scholarly journals Phytochemicals of Minthostachys diffusa Epling and Their Health-Promoting Bioactivities

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 144
Author(s):  
Immacolata Faraone ◽  
Daniela Russo ◽  
Lucia Chiummiento ◽  
Eloy Fernandez ◽  
Alka Choudhary ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Mass Spectrometry Analysis ◽  
Antioxidant Power ◽  
Spectrometry Analysis ◽  
In Silico Docking ◽  
Aerial Parts ◽  
Health Promoting

The genus Minthostachys belonging to the Lamiaceae family, and is an important South American mint genus used commonly in folk medicine as an aroma in cooking. The phytochemical-rich samples of the aerial parts of Minthostachys diffusa Epling. were tested for pharmacological and health-promoting bioactivities using in vitro chemical and enzymatic assays. A range of radical scavenging activities of the samples against biological radicals such as nitric oxide and superoxide anion and against synthetic 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals, the ferric reducing antioxidant power and the lipid peroxidation inhibition were determined and ranked using the ‘relative antioxidant capacity index’ (RACI). The ethyl acetate fraction showed the highest RACI of +1.12. Analysis of the various fractions’ inhibitory ability against enzymes involved in diabetes (α-amylase and α-glucosidase), and against enzymes associated with Parkinson’s or Alzheimer’s diseases (acetylcholinesterase and butyrylcholinesterase) also suggested that the ethyl acetate fraction was the most active. Liquid chromatography–tandem mass spectrometry analysis of the ethyl acetate fraction showed more than 30 polyphenolic compounds, including triterpenes. The inhibitory cholinesterase effects of the triterpenes identified from M. diffusa were further analysed by in silico docking of these compounds into 3D-structures of acetylcholinesterase and butyrylcholinesterase. This is the first study on pharmacological activities and phytochemical profiling of the aerial parts of M. diffusa, showing that this plant, normally used as food in South America, is also rich in health-promoting phytochemicals.

scholarly journals Human Neuronal Cell Lines as An In Vitro Toxicological Tool for the Evaluation of Novel Psychoactive Substances

2021 ◽  
Vol 22 (13) ◽  
pp. 6785
Author(s):  
Valeria Sogos ◽  
Paola Caria ◽  
Clara Porcedda ◽  
Rafaela Mostallino ◽  
Franca Piras ◽  
...  
Keyword(s):  
Cell Death ◽  
Neuronal Cell ◽  
In Vitro Study ◽  
Dependent Manner ◽  
Novel Psychoactive Substances ◽  
Psychoactive Substances ◽  
Concentration Dependent Manner ◽  
Mechanisms Of Toxicity ◽  
Neuronal Cell Lines

Novel psychoactive substances (NPS) are synthetic substances belonging to diverse groups, designed to mimic the effects of scheduled drugs, resulting in altered toxicity and potency. Up to now, information available on the pharmacology and toxicology of these new substances is very limited, posing a considerable challenge for prevention and treatment. The present in vitro study investigated the possible mechanisms of toxicity of two emerging NPS (i) 4′-methyl-alpha-pyrrolidinoexanophenone (3,4-MDPHP), a synthetic cathinone, and (ii) 2-chloro-4,5-methylenedioxymethamphetamine (2-Cl-4,5-MDMA), a phenethylamine. In addition, to apply our model to the class of synthetic opioids, we evaluated the toxicity of fentanyl, as a reference compound for this group of frequently abused substances. To this aim, the in vitro toxic effects of these three compounds were evaluated in dopaminergic-differentiated SH-SY5Y cells. Following 24 h of exposure, all compounds induced a loss of viability, and oxidative stress in a concentration-dependent manner. 2-Cl-4,5-MDMA activates apoptotic processes, while 3,4-MDPHP elicits cell death by necrosis. Fentanyl triggers cell death through both mechanisms. Increased expression levels of pro-apoptotic Bax and caspase 3 activity were observed following 2-Cl-4,5-MDMA and fentanyl, but not 3,4-MDPHP exposure, confirming the different modes of cell death.

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