scholarly journals Characterization of Bacterial Etiologic Agents of Biofilm Formation in Medical Devices in Critical Care Setup

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Sangita Revdiwala ◽  
Bhaumesh M. Rajdev ◽  
Summaiya Mulla
Keyword(s):  
Critical Care ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Medical Implants ◽  
Bacterial Isolates ◽  
Critical Care Units ◽  
Etiologic Agents ◽  
Bacterial Drug Resistance ◽  
Biofilm Development

Background. Biofilms contaminate catheters, ventilators, and medical implants; they act as a source of disease for humans, animals, and plants.Aim. Critical care units of any healthcare institute follow various interventional strategies with use of medical devices for the management of critical cases. Bacteria contaminate medical devices and form biofilms.Material and Methods. The study was carried out on 100 positive bacteriological cultures of medical devices which were inserted in hospitalized patients. The bacterial isolates were processed as per microtitre plate. All the isolates were subjected to antibiotic susceptibility testing by VITEK 2 compact automated systems.Results. Out of the total 100 bacterial isolates tested, 88 of them were biofilm formers. A 16–20-hour incubation period was found to be optimum for biofilm development. 85% isolates were multidrug resistants and different mechanisms of bacterial drug resistance like ESBL, carbapenemase, and MRSA were found among isolates.Conclusion. Availability of nutrition in the form of glucose enhances the biofilm formation by bacteria. Time and availability of glucose are important factors for assessment of biofilm progress. It is an alarm for those who are associated with invasive procedures and indwelling medical devices especially in patients with low immunity.

scholarly journals Preventing Biofilm Formation and Development on Ear, Nose and Throat Medical Devices

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1025
Author(s):  
Dan Cristian Gheorghe ◽  
Andrei Ilie ◽  
Adelina-Gabriela Niculescu ◽  
Alexandru Mihai Grumezescu
Keyword(s):  
Medical Devices ◽  
Biofilm Formation ◽  
Microbial Colonization ◽  
Long Time ◽  
3D Matrix ◽  
Common Problems ◽  
Underlying Mechanisms ◽  
Surface Modification Techniques ◽  
Biofilm Development ◽  
Nose And Throat

Otorhinolaryngology is a vast domain that requires the aid of many resources for optimal performance. The medical devices utilized in this branch share common problems, such as the formation of biofilms. These structured communities of microbes encased in a 3D matrix can develop antimicrobial resistance (AMR), thus making it a problem with challenging solutions. Therefore, it is of concern the introduction in the medical practice involving biomaterials for ear, nose and throat (ENT) devices, such as implants for the trachea (stents), ear (cochlear implants), and voice recovery (voice prosthetics). The surface of these materials must be biocompatible and limit the development of biofilm while still promoting regeneration. In this respect, several surface modification techniques and functionalization procedures can be utilized to facilitate the success of the implants and ensure a long time of use. On this note, this review provides information on the intricate underlying mechanisms of biofilm formation, the large specter of implants and prosthetics that are susceptible to microbial colonization and subsequently related infections. Specifically, the discussion is particularized on biofilm development on ENT devices, ways to reduce it, and recent approaches that have emerged in this field.

Does getting high hurt? Characterization of cases of LSD and psilocybin-containing mushroom exposures to national poison centers between 2000 and 2016

2018 ◽  
Vol 32 (12) ◽  
pp. 1286-1294 ◽  
Author(s):  
James B Leonard ◽  
Bruce Anderson ◽  
Wendy Klein-Schwartz
Keyword(s):  
Emergency Department ◽  
Critical Care ◽  
Clinical Effects ◽  
Medical Outcomes ◽  
Critical Care Units ◽  
Large Populations ◽  
Emergency Department Management ◽  
Poison Centers ◽  
Psychiatric Conditions

Background: Lysergic acid diethylamide (LSD) and psilocybin are serotonergic hallucinogens that are used primarily for recreational abuse. Small studies evaluated the efficacy of LSD and psilocybin for several psychiatric conditions. There are limited safety or toxicity data for either of these substances, especially in large populations. Methods: This was a retrospective analysis of single-substance exposures of LSD or psilocybin-containing mushrooms (PcMs) reported to United States poison centers from 1 January 2000 to 31 December 2016. The study describes the most frequent toxicities, management sites, and medical outcomes. Results: A total of 5883 PcM and 3554 LSD exposures were included. Most patients were between 13 and 29 years of age (83.9% PcM, 88.9% LSD) and primarily male (77.9% PcM, 74.1% LSD). Most common clinical effects were hallucinations (45.8% PcM, 37.4% LSD), agitation (24.1% PcM, 42.4% LSD), and tachycardia (18.0% PcM, 38.6% LSD). Serious clinical effects were infrequent, but included hyperthermia, seizures, coma, increased serum creatinine, and cardiac arrest. Most patients were treated and released from the emergency department. More LSD patients were admitted to critical care and non-critical care units than PcM patients. Moderate effect was the most frequent outcome for both substances (61.0% PcM, 62.3% LSD). Conclusion: These data find that LSD and PcM use occurs primarily in adolescents and young adults, who experience mild to moderate adverse effects. Serious effects are infrequent but can occur. While most LSD and PcM users require only emergency department management, LSD use is more likely to require medical admission.

scholarly journals Effective Prevention of Microbial Biofilm Formation on Medical Devices by Low-Energy Surface Acoustic Waves

2006 ◽  
Vol 50 (12) ◽  
pp. 4144-4152 ◽  
Author(s):  
Zadik Hazan ◽  
Jona Zumeris ◽  
Harold Jacob ◽  
Hanan Raskin ◽  
Gera Kratysh ◽  
...  
Keyword(s):  
Medical Devices ◽  
Acoustic Waves ◽  
Biofilm Formation ◽  
Energy Surface ◽  
Surface Acoustic Waves ◽  
Low Energy ◽  
Microbial Biofilm ◽  
Effective Prevention ◽  
Biofilm Development ◽  
Power Densities

ABSTRACT Low-energy surface acoustic waves generated from electrically activated piezo elements are shown to effectively prevent microbial biofilm formation on indwelling medical devices. The development of biofilms by four different bacteria and Candida species is prevented when such elastic waves with amplitudes in the nanometer range are applied. Acoustic-wave-activated Foley catheters have all their surfaces vibrating with longitudinal and transversal dispersion vectors homogeneously surrounding the catheter surfaces. The acoustic waves at the surface are repulsive to bacteria and interfere with the docking and attachment of planktonic microorganisms to solid surfaces that constitute the initial phases of microbial biofilm development. FimH-mediated adhesion of uropathogenic Escherichia coli to guinea pig erythrocytes was prevented at power densities below thresholds that activate bacterial force sensor mechanisms. Elevated power densities dramatically enhanced red blood cell aggregation. We inserted Foley urinary catheters attached with elastic-wave-generating actuators into the urinary tracts of male rabbits. The treatment with the elastic acoustic waves maintained urine sterility for up to 9 days compared to 2 days in control catheterized animals. Scanning electron microscopy and bioburden analyses revealed diminished biofilm development on these catheters. The ability to prevent biofilm formation on indwelling devices and catheters can benefit the implanted medical device industry.

scholarly journals Abstracts of an international conference “Biofilms II: Attachment and detachment in pure and mixed cultures”, held at the Leipziger Kubus UFZ Centre for Environmental Research, Leipzig, Germany, from 23 March to 24 March 2006

Biofilms ◽  
2005 ◽  
Vol 2 (4) ◽  
pp. 245-273
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Three Dimensional ◽  
Conjugative Plasmid ◽  
Environmental Research ◽  
Biofilm Growth ◽  
Biofilm Detachment ◽  
Biofilm Development

The effect of growth and detachment on formation of large-scale biofilm structureBiofilm cohesive energy density determination using a novel atomic force microscopy methodologyFluorescence correlation spectroscopy under two-photon excitation for the study of diffusion and reactivity of bacteriophage inside bacterial biofilmsBiothermodynamic characterization and dynamic analysis of biofilms using calorimetryBiomimetic antifouling coatings for sensor surfaces for water monitoring: performance control in defined biofilm cultures and under real environmental conditionsThe contribution of rpos to formation of Escherichia coli biofilmsSynergistic effects in mixed Escherichia coli biofilms: conjugative plasmid transfer drives biofilm expansionThe universal stress protein PA3309 in Pseudomonas aeruginosa is induced in biofilmsExtracellular polymeric substances from biofilms on membranes in waste-water treatment plantsBiofilm-to-planktonic cell yield: a strategy for proliferationPhysiological and phylogenetic characterization of the dispersed and loosely attached fraction of activated sludge flocsTowards a deterministic model of biofilm detachment: an experimental studyEffect of backwash on the characteristics of biofilm in a biological activated filter reactor using elemental sulfur particlesProcess performance and biomass properties in membrane-aerated bioreactorsBioaugmentation via conjugation in biofilms treating 3-chloroaniline: effects of selective pressureEffect of phosphorus on biofilm growth in a completely mixed biofilm reactorImpacts of biofilm development on reactive transport in porous media under variable flow regimensInfluence of biofilms on colloid mobility in the subsurfaceBiofilms in amendable in situ microcosms indicate relevant electron acceptor processes at a BTEX-contaminated aquiferFunctional biodiversity of complex biofilms grown on polychlorinated biphenyl oilIdentification and characterization of biofilm formation phenotypes of several clinically relevant Streptococcus pyogenes serotype strainsSelected probiotic bacteria disrupt biofilm development of vancomycin-resistant Enterococcus faeciumComparison of the extracellular polymeric substances of Candida albicans and Candida dubliniensis biofilmsInfluence of quorum-sensing regulated production of an antimicrobial component by Serratia plymuthica on establishment of dual species biofilms with Escherichia coliBiofilm formation by the thermophilic and cellulolytic actinomycete Thermobifida fuscaBiomonitoring of bacterial contamination on different surfaces of food-processing machinesRole of the flagella during the adhesion of Listeria monocytogenes EGD-e to inert surfaces after cultivation at different pHs and temperaturesAdhesion of Saccharomyces cerevisiae to stainless steel: influence of surface propertiesInvestigating the mechanical strength of biofilms with fluid dynamic gaugingThree-dimensional biofilm model with individual cells and continuum extracellular polymeric substances matrixA three-dimensional computer model analysis of four hypothetical biofilm detachment mechanismsModelling biofilm growth, detachment and fluid flow in a cross-section of tube reactorsBiofilm games

Unplanned Removal of Medical Devices in Critical Care Units in North West England Between 2011 and 2016

2019 ◽  
Vol 28 (3) ◽  
pp. 213-221 ◽  
Author(s):  
Joanna E. Balmforth ◽  
Antony N. Thomas
Keyword(s):  
Critical Care ◽  
Medical Devices ◽  
Nasogastric Tube ◽  
Device Removal ◽  
Patient Factors ◽  
North West ◽  
Critical Care Units ◽  
Incident Reports ◽  
Manual Handling ◽  
Central Catheters

Background The unplanned removal of medical devices poses a risk of harm to critically ill patients. Objective To determine rates, causes, and consequences of unplanned medical device removal, as well as factors mitigating harm to patients, in critical care units in the United Kingdom by reviewing patient safety incident reports. Methods Incidents of unplanned medical device removal in critical care units in North West England between 2011 and 2016 were retrospectively reviewed and classified. The incidents were classified by type of device displaced, staff and patient factors, causes and consequences of removal, and staff actions following removal. Displacement rates were calculated per 1000 patient days per unit. Results A total of 34 705 incident reports were reviewed, of which 1090 described unplanned device removal. The median rate of device removal was 0.7 (interquartile range, 0.4-2.2) per 1000 patient days per unit. Devices displaced most commonly included nasogastric tubes (317), central catheters (245), tracheostomy tubes (174), and endotracheal tubes (140). A total of 11 cardiac arrests were reported (8 associated with airway devices and 3 with central catheters). Factors contributing to displacement included initial placement (188), patient factors (563), and manual handling (238). Manual handling was cited in 49% of central catheter incidents and only 9% of nasogastric tube incidents. Patients’ organic confusion was a factor in 16% of endotracheal tube and 80% of nasogastric tube removals. Conclusions Unplanned device removal may cause patient harm and is often preventable. The causes and consequences depend on the type of device removed.

scholarly journals Inhibition of Staphylococcus epidermidis Biofilm by Trimethylsilane Plasma Coating

2012 ◽  
Vol 56 (11) ◽  
pp. 5923-5937 ◽  
Author(s):  
Yibao Ma ◽  
Meng Chen ◽  
John E. Jones ◽  
Andrew C. Ritts ◽  
Qingsong Yu ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Medical Devices ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Plasma Coating ◽  
Bacterial Cells ◽  
Implantable Medical Devices ◽  
Content Type ◽  
Coated Surfaces ◽  
Biofilm Development

ABSTRACTBiofilm formation on implantable medical devices is a major impediment to the treatment of nosocomial infections and promotes local progressive tissue destruction.Staphylococcus epidermidisinfections are the leading cause of biofilm formation on indwelling devices. Bacteria in biofilms are highly resistant to antibiotic treatment, which in combination with the increasing prevalence of antibiotic resistance among human pathogens further complicates treatment of biofilm-related device infections. We have developed a novel plasma coating technology. Trimethylsilane (TMS) was used as a monomer to coat the surfaces of 316L stainless steel and grade 5 titanium alloy, which are widely used in implantable medical devices. The results of biofilm assays demonstrated that this TMS coating markedly decreasedS. epidermidisbiofilm formation by inhibiting the attachment of bacterial cells to the TMS-coated surfaces during the early phase of biofilm development. We also discovered that bacterial cells on the TMS-coated surfaces were more susceptible to antibiotic treatment than their counterparts in biofilms on uncoated surfaces. These findings suggested that TMS coating could result in a surface that is resistant to biofilm development and also in a bacterial community that is more sensitive to antibiotic therapy than typical biofilms.

C-di-GMP Pathway in Biomining Bacteria

2009 ◽  
Vol 71-73 ◽  
pp. 223-226 ◽  
Author(s):  
M. Castro ◽  
Lina María Ruíz ◽  
A. Barriga ◽  
Carlos A. Jerez ◽  
David S. Holmes ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Molecular Mechanisms ◽  
Bacterial Attachment ◽  
Effector Proteins ◽  
Community Based ◽  
Gram Negative ◽  
Cell Extracts ◽  
Diguanylate Cyclases ◽  
Biofilm Development

Acidithiobacillus ferrooxidans, A. thiooxidans, and A. caldus are acidophilic Gram-negative -proteobacteria involved in the bioleaching of metal sulfides. Bacterial attachment to mineral surface and biofilm development play a pivotal role in this process. Therefore, the understanding of biofilm formation has relevance to the design of biological strategies to improve the efficiency of bioleaching processes. For this reason, our laboratory is focused on the characterization of the molecular mechanisms involved in biofilm formation in biomining bacteria. In many bacteria, the intracellular level of c-di-GMP molecules regulates the transition from the motile planktonic state to sessile community-based behaviors, such as biofilm development. Thus, we recently started the study of c-di-GMP pathway in biomining bacteria. C-di-GMP molecules are synthesized by diguanylate cyclases (DGCs) and degraded by phosphodiesterases (PDEs). So far, two kinds of effectors have been identified, including three protein families (pilZ, PleD and FleQ) and a conserved RNA domain (GEMM) which acts as a riboswitch. We previously reported the existence of different molecular players involved in c-di-GMP pathway in A. ferrooxidans ATCC 23270. Here, we expanded our work to other Acidithioibacillus species: A. thiooxidans ATCC 19377 and A. caldus ATCC 51756. In both, we identified several putative-ORFs encoding DGC, PDE and effector proteins. By using total RNA extracted from A. ferrooxidans and A. caldus cells in RT-PCR and qPCR experiments, we demonstrated that these genes are expressed. In addition, we characterized the presence of c-di-GMP in A. ferrooxidans ATCC 23270 and A. caldus ATCC 51756 cell extracts. Taken together, these results strongly suggest that A. ferrooxidans, A. caldus and A. thiooxidans possess functional c-di-GMP pathways. As it occurs in other Gram-negative bacteria, this pathway should be involved in the regulation of the planktonic/biofilm switch. In the future, we have to integrate this new biological dimension to improve the biological understanding of bioleaching.

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