scholarly journals Posttonsillectomy Bacteremia and Comparison of Tonsillar Surface and Deep Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-5
Author(s):  
Mahmood Shishegar ◽  
Mohammad Javad Ashraf
Keyword(s):  
Venous Blood ◽  
Blood Cultures ◽  
Chronic Tonsillitis ◽  
Blood Samples ◽  
Hemolytic Streptococcus ◽  
Microbiological Study ◽  
Common Organism ◽  
Before And After ◽  
Deep Culture ◽  
Time Of Operation

Objective.This study aimed to identify the microorganisms of surface and depth of tonsils and whether these microorganisms bring the menace of bacteremia during tonsillectomy in the children under surgery.Materials and Methods.The culture specimens were taken from surface and depth of tonsil from the patients suffering from chronic tonsillitis at the time of operation. Also, 10 mL venous blood samples were taken 5 minutes before and after the operation for microbiological study.Results.According to the results, 112 (76.1%) and 117 (79.6%) cultures from surface and depth of tonsils represented multiple microorganisms, respectively. Besides, staphylococci coagulase positive was the most common organism in both surface and depth of tonsils. None of the preoperation blood cultures were positive, while 3 postoperation blood cultures (2.1%) were positive. Staphylococci coagulase negative and alpha hemolytic streptococcus were detected in 2 cases (1.4%) and 1 case (0.7%), respectively.Conclusion.In the present study, the two cultured sites were almost similar regarding the types of isolated microorganisms. Our results suggested that bacteremia might occur after tonsillectomy. Therefore, to avoid the possible dramatic outcomes after tonsillectomy, pre- and postoperation attendances are essential.

ADRENAL 17-HYDROXYCORTICOSTEROID SECRETION IN THE DOG IN RESPONSE TO ETHANOL

1972 ◽  
Vol 70 (4) ◽  
pp. 736-740 ◽  
Author(s):  
T. Suzuki ◽  
R. Higashi ◽  
T. Hirose ◽  
H. Ikeda ◽  
K. Tamura
Keyword(s):  
Venous Blood ◽  
Secretion Rate ◽  
Conscious Dogs ◽  
Blood Samples ◽  
Before And After

ABSTRACT Conscious dogs were infused intravenously with ethanol in doses of 0.7 and 1.0 g/kg. The adrenal venous blood samples were collected before and after the infusion of ethanol and analysed for 17-hydroxycorticosteroids (17-OHCS). After the infusion of 0.7 g/kg (subanaesthetic dose) of ethanol the adrenal 17-OHCS secretion rate showed either a slight increase or no change. After the infusion of 1.0 g/kg (anaesthetic dose) of ethanol the adrenal 17-OHCS secretion rate increased markedly and reached 1.21±0.15 (mean±sem) μg/kg/min, while it was 0.09±0.023 μg/kg/min before the infusion.

Plasma catecholamines and their effect on blood lactate and muscle lactate output

1984 ◽  
Vol 57 (2) ◽  
pp. 321-325 ◽  
Author(s):  
W. N. Stainsby ◽  
C. Sumners ◽  
G. M. Andrew
Keyword(s):  
Venous Blood ◽  
Plasma Catecholamines ◽  
Muscle Group ◽  
O2 Uptake ◽  
Plantaris Muscle ◽  
Muscle Lactate ◽  
Blood Samples ◽  
Lactate Output ◽  
Before And After

This study was designed to test the hypothesis that epinephrine (E) and norepinephrine (NE) increase net muscle lactate output (L) of in situ gastrocnemius-plantaris muscle group during contractions. Plasma [E] and [NE] were measured before and after the surgical isolation of the muscle and at 10-min intervals during the 60-min experiments. Plasma [E] and [NE] were increased threefold by intravenous infusions of E (n = 3) or NE (n = 3) at a rate of 1.5 micrograms X kg body wt-1 X min-1. Arterial and muscle venous blood samples for O2 and lactate concentrations were also obtained. The infusions began at min 11 and repetitive isometric contractions (4 tw/s) began at min 31. The presurgery plasma [E] and [NE] averaged 0.34 and 0.52 ng/ml, respectively, and rose to 1.12 and 1.19 ng/ml 10 min after surgery. Arterial and venous lactate concentrations (CaL and CvL) increased continuously during E infusion but remained constant during NE infusion. Maximal L during the first 10 min of contractions was significantly increased compared with an identical earlier study without infusions. O2 uptake was not changed by the infusions. It is concluded that E causes CaL to rise and that both E and NE increase maximal net lactate output during contractions.

Free Radicals and Scavenging Enzymes in Chronic Tonsillitis

2003 ◽  
Vol 129 (3) ◽  
pp. 265-268 ◽  
Author(s):  
Irfan Kaygusuz ◽  
Nevin Ilhan ◽  
Turgut Karlidag ◽  
Erol Keles ◽  
Sinasi Yalçin ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Oxygen Radicals ◽  
Venous Blood ◽  
Chronic Tonsillitis ◽  
Free Oxygen Radicals ◽  
Free Oxygen ◽  
Blood Samples ◽  
Tonsil Tissue ◽  
Scavenging Enzymes ◽  
The Relationship

OBJECTIVE: This study aimed to define the relationship between chronic tonsillitis and levels of malondialdehyde and superoxide dismutase in free radical and antioxidant forms. It is suggested that free oxygen radicals may play a role in chronic tonsillitis. MATERIALS AND METHODS: One hundred twenty-four patients were enrolled in the study. Tonsillectomy was performed via the usual dissection-snare method. Venous blood was taken preoperatively and at 2 weeks postoperatively. Blood samples and tonsil specimens were evaluated for malondialdehyde and superoxide dismutase analysis. RESULTS: The levels of malondialdehyde and superoxide dismutase in plasma were compared preoperatively and postoperatively, and there were statistically significant differences between these levels ( P < 0.05). In contrast, the levels of malondialdehyde and superoxide dismutase in tonsil tissue were not correlated with the plasma levels of malondialdehyde and superoxide dismutase in pretonsillectomy and posttonsillectomy terms ( P > 0.05). CONCLUSION: The presence of malondialdehyde and superoxide dismutase in plasma and tonsil tissue reinforces the involvement of oxidative stress in the pathophysiology of chronic tonsillitis.

scholarly journals Micro-RNAs miR-375-3p and miR-7-5p are released alongside ACTH from corticotroph pituitary neuroendocrine tumor

2021 ◽  
Author(s):  
Helvijs Niedra ◽  
Raitis Peculis ◽  
Ilze Konrade ◽  
Inga Balcere ◽  
Mihails Romanovs ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Venous Blood ◽  
Differential Expression Analysis ◽  
Circulating Mirnas ◽  
Blood Samples ◽  
Peripheral Plasma ◽  
Bodily Fluids ◽  
Micro Rnas ◽  
Before And After ◽  
Acth Secreting

Objective: Circulating miRNAs are found in bodily fluids including plasma and can serve as biomarkers for diseases. The aim of this study was to provide the first insight into the landscape of circulating miRNAs in close proximity to the adrenocorticotropic hormone (ACTH) secreting PitNET. To achieve this objective next-generation sequencing of miRNAs in plasma from bilateral inferior petrosal sinus sampling (BIPSS) - a gold standard in diagnosing ACTH-secreting PitNETs, was carried out. Methods: Sinistral (left) and dextral (right) BIPSS blood samples of the patient were collected in three time points: before the administration of corticotropin-releasing hormone, 5 and 15 minutes after stimulation. Peripheral venous blood samples were also collected 24 hours before and after BIPSS and before the resection of PitNET and 24 hours after. In differential expression analysis sinistral plasma was compared with dextral. Results: BIPSS concluded that the highest amount of ACTH was released in the sinistral side at the 5th minute mark indicating a presence of tumor. The highest amount of differentially expressed miRNAs was observed 5 minutes after stimulation (20 upregulated, 14 downregulated). At the 5th minute mark in sinistral plasma, two miRNAs were identified: hsa-miR-7-5p and hsa-miR-375-3p that were highly upregulated compared to other BIPSS samples and peripheral plasma samples. Clustering analysis showed that BIPSS plasma differs from peripheral plasma in miRNA expression patterns. Conclusions: data indicates that ACTH-secreting PitNET actively releases two circulating miRNAs upon stimulation with CRH (hsa-mir-7-5p, hsa-mir-375-3p) alongside with ACTH implying further studies of these miRNA as diagnostic markers are needed.

Acute exercise improves serum adiponectin not leptin in sedentary adult obese men

2012 ◽  
Vol 1 (6) ◽  
pp. 298-304
Author(s):  
Eizadi Mojtaba ◽  
Kohandel Mahdi ◽  
Kasbparast JR Mehdi ◽  
Sarshin Amir
Keyword(s):  
Acute Exercise ◽  
Venous Blood ◽  
Serum Adiponectin ◽  
Acute Bout ◽  
Blood Samples ◽  
Serum Leptin ◽  
Before And After ◽  
Obese Subjects ◽  
Short Time ◽  
Inflammation Cytokines

 Leptin and adiponectin, adipose tissue secreted cytokines, play key role inobesity and cardiovascular disease. Although the physiopathological mechanisms underlying these associations are largely unknown. Venous blood samples were obtained before and after an acute bout of moderate cycling test in eighty non‐trained adult obese men (BMI: 33.54 ± 3.43 kg/m2) that participated in this study by accidentally. Blood samples were used for measuring serum leptin and adiponectin. No significant differences were found in serum leptin by cycling exercise with compared to baseline (P ≥ 0.05). But, serum adiponectin levels were significantly increased in response to acute exercise when compared with baseline levels (P < 0.05). Based on these data, we can say, although inflammation cytokines such as leptin does not affect by acute exercise for short time, but it seems that this exercise can increase anti‐inflamatory cytokines as adiponectin in obese subjects. The findings of this study indicate the fact that in response to short‐term exercise, changes in serum adiponectin levels are independent of the leptin response.

scholarly journals The effect of insulin on the dextrose consumption perfused skeletal muscle

1926 ◽  
Vol 99 (698) ◽  
pp. 375-382 ◽  
Keyword(s):  
Skeletal Muscle ◽  
Femoral Artery ◽  
Clinical Studies ◽  
Venous Blood ◽  
Laboratory Animals ◽  
Blood Samples ◽  
Mammalian Heart ◽  
Hind Limbs ◽  
Before And After ◽  
Marked Resistance

The experiments of Hepburn and Latchford (1), which have been confirmed by Burn and Dale (2), show that insulin accelerates the rate of disappearance of dextrose from the fluid used to perfuse the isolated mammalian heart. Burn and Dale also demonstrated that insulin greatly increases the rate of disappearance of dextrose from the circulating blood of the decapitated and eviscerated cat. Cori, Cori and Goltz (3), working on rabbits/ and Lawrence (4) and Pemberton and Cunningham (5), from clinical studies, have reported that insulin increases the loss of sugar from the blood during its passage through a limb. Frank, Nothman and Wagner (6) have obtained similar results by analyses of blood samples drawn simultaneously from the femoral artery and vein, after the injection of insulin into the femoral artery. Macleod (7) states that, in experiments in his laboratory, no increased discrepancy between the dextrose content of the arterial and venous blood was observed after the administration of insulin in normal or diabetic animals. Attempts to prove that insulin causes an increased disappearance of sugar from the fluid perfused through the isolated limbs of laboratory animals have been made by Macleod and his collaborators (7) and Staub (8). Macleod states that his experiments were unsatisfactory because of œdema of the muscles or the development of marked resistance to the perfusion. Staub has reported experiments in which the rate of sugar disappearance, before and after the addition of insulin, from the defibrinated blood used to perfuse the hind limbs of the dog, are recorded. In some of Staub’s experiments insulin appeared definitely to accelerate the sugar disappearance. Because of the very rapid disappearance of sugar from the blood before the addition of insulin, however, it is difficult to demonstrate convincingly, by this type of experiment, that the rate of disappearance is really accelerated by insulin.

Bacteriemia During Mastoidectomy and/or Tympanoplasty

2005 ◽  
Vol 133 (3) ◽  
pp. 347-351 ◽  
Author(s):  
Erol Keleş ◽  
Ahmet Kizirgil ◽  
Irfan Kaygusuz ◽  
Turgut Karlidaĝ ◽  
Şinasi Yalçin ◽  
...  
Keyword(s):  
Otitis Media ◽  
Microbial Growth ◽  
Venous Blood ◽  
Chronic Otitis Media ◽  
Blood Cultures ◽  
Preoperative Period ◽  
Ear Canal ◽  
Before And After ◽  
Graft Success ◽  
Pressure Dressing

OBJECTIVE: To investigate the risk of bacteriemia development during surgery in patients who underwent mastoidectomy and/or tympanoplasty for chronic otitis media. PATIENTS AND METHODS: A total of 59 patients with chronic otitis media who were undergoing tympanoplasty operation with or without mastoidectomy were enrolled in this study. Smear cultures were obtained from outer ear canal and/or middle ears of all patients before the operation. Venous blood samples were obtained before and after the operation for bacteriologic analysis. Smear cultures were also obtained from the pressure dressing material that was applied during the operation for retrieval of the outer ear canal pressure. RESULTS: There was a 13.5% difference between the outer ear canal and/or middle ear smear cultures and ear pressure dressing smear cultures of the same case. There was no growth in the blood cultures obtained before tympanoplasty in any of the involved cases, however, in 5 (8.4%) of the culture samples obtained immediately after the tympanoplasty operation, there was bacteriemia. In the pressure dressing smear cultures obtained after the operation, 11 patients had microbial growth. CONCLUSION: Risk of bacteriemia should be considered in the preoperative period for the patients undergoing mastoidectomy especially in patients with cardiovascular diseases, this is of importance for the dramatic consequences that might arise after the operation. We also think that bacteriemia is also one of the factors that influences graft success rate.

Effect of hypothermia on the secretion of immunoreactive insulin in response to glucose

1968 ◽  
Vol 46 (3) ◽  
pp. 411-415 ◽  
Author(s):  
B. J. Lin ◽  
J. Hunter ◽  
R. Weldon ◽  
R. E. Haist
Keyword(s):  
Venous Blood ◽  
Insulin Response ◽  
Immunoreactive Insulin ◽  
Rapid Rise ◽  
Blood Samples ◽  
Insulin Levels ◽  
Glucose Levels ◽  
Glucose Injection ◽  
Before And After ◽  
Sugar Levels

Insulin levels in the serum of peripheral venous blood samples from normothermic (37 °C) and hypothermic (24.5 ± 0.5 °C) dogs were estimated by immunoassay before and after the intravenous injection of glucose. In contrast to the rapid rise and fall of glucose levels and insulin levels in normothermic dogs following glucose injection, in most of the hypothermic dogs blood sugar levels rose and were sustained above normal levels throughout the test. These changes were accompanied by elevations in blood insulin levels which were sustained or progressively increased. Several hypothermic animals showed a reduced insulin response despite similar changes in blood glucose.

Transcerebral net exchange of vasoactive peptides and catecholamines during lipopolysaccharide-induced systemic inflammation in healthy humans

2018 ◽  
Vol 96 (3) ◽  
pp. 313-316
Author(s):  
Ronan M.G. Berg ◽  
Sarah Taudorf ◽  
Damian M. Bailey ◽  
Rasmus H. Dahl ◽  
Carsten Lundby ◽  
...  
Keyword(s):  
Venous Blood ◽  
Blood Samples ◽  
Fick Principle ◽  
Vasoactive Peptides ◽  
Healthy Humans ◽  
Cerebral Vasoconstriction ◽  
Calcitonin Gene ◽  
Human Volunteers ◽  
Before And After ◽  
Underlying Mechanisms

The systemic inflammatory response triggered by lipopolysaccharide (LPS) is associated with cerebral vasoconstriction, but the underlying mechanisms are unknown. We therefore examined whether a 4-hour intravenous LPS infusion (0.3 ng·kg−1) induces any changes in the transcerebral net exchange of the vasoactive peptides endothelin-1 (ET-1) and calcitonin-gene related peptide (CGRP) and catecholamines in human volunteers. Cerebral blood flow was measured by the Kety–Schmidt technique, and paired arterial-to-jugular venous blood samples were obtained for estimating the transcerebral exchange of ET-1, CGRP, and catecholamines by the Fick principle in 12 volunteers before and after LPS infusion. The cerebrovascular release of ET-1 was enhanced, whereas the transcerebral net exchange of CGRP and catecholamines was unaffected. Our findings thus point towards locally produced ET-1 within the cerebrovasculature as a contributor to cerebral vasoconstriction after LPS infusion.

Influence of a Carbohydrate-Electrolyte Beverage on Performance and Blood Homesstasis during Recovery from Football

1991 ◽  
Vol 1 (2) ◽  
pp. 178-191 ◽  
Author(s):  
David Criswell ◽  
Scott Powers ◽  
John Lawler ◽  
John Tew ◽  
Stephen Dodd ◽  
...  
Keyword(s):  
Venous Blood ◽  
Anaerobic Exercise ◽  
Football Players ◽  
Anaerobic Performance ◽  
High School Football ◽  
Blood Samples ◽  
Glucose Polymer ◽  
Before And After ◽  
Maximal Effort ◽  
Percent Decrease

This study compared the efficacy of a 7% glucose polymer beverage containing electrolytes (GP) versus a nonnutrient, nonelectrolyte placebo (P) in maintaining blood homeostasis during recovery from football and determined whether consumption of the GP beverage improved anaerobic performance immediately after football competition when compared with the placebo. Forty-four high school football players participated in a 50-play scrimmage designed to simulate game conditions. At each of six periods before and during the scrimmage, players consumed 170 ml of the GP or P beverage. Eight maximal-effort 40-yd sprints (40-sec rest intervals) were performed before and after the scrimmage to assess the decrement in anaerobic performance from the scrimmage. Venous blood samples were drawn before and after the scrimmage and analyzed. The pre- to postscrimmage differences in mean and peak sprint velocities did not differ between treatments, nor did body weight and plasma. In contrast, the percent decrease in plasma volume was significantly greater in the P group. Postscrimmage increases in glucose and insulin were greater in the GP group. These data suggest that CHO-electrolyte drinks do not prevent a decline in anaerobic performance when compared to water, but a CMO-electrolyte drink is more effective in maintaining PV than water during recovery from anaerobic exercise.

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