Oxidative Damage to Nucleic Acids and Benzo(a)pyrene-7,8-diol-9,10-epoxide-DNA Adducts and Chromosomal Aberration in Children with Psoriasis Repeatedly Exposed to Crude Coal Tar Ointment and UV Radiation

The paper presents a prospective cohort study. Observed group was formed of children with plaque psoriasis (n=19) treated by Goeckerman therapy (GT). The study describes adverse (side) effects associated with application of GT (combined exposure of 3% crude coal tar ointment and UV radiation). After GT we found significantly increased markers of oxidative stress (8-hydroxy-2′-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine), significantly increased levels of benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) DNA adducts (BPDE-DNA), and significantly increased levels of total number of chromosomal aberrations in peripheral lymphocytes. We found significant relationship between (1) time of UV exposure and total number of aberrated cells and (2) daily topical application of 3% crude coal tar ointment (% of body surface) and level of BPDE-DNA adducts. The findings indicated increased hazard of oxidative stress and genotoxic effects related to the treatment. However, it must be noted that the oxidized guanine species and BPDE-DNA adducts also reflect individual variations in metabolic enzyme activity (different extent of bioactivation of benzo[a]pyrene to BPDE) and overall efficiency of DNA/RNA repair system. The study confirmed good effectiveness of the GT (significantly decreased PASI score).

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Defects in the Error Prevention Oxidized Guanine System Potentiate Stationary-Phase Mutagenesis in Bacillus subtilis

Journal of Bacteriology ◽

10.1128/jb.01210-08 ◽

2008 ◽

Vol 191 (2) ◽

pp. 506-513 ◽

Cited By ~ 26

Author(s):

Luz E. Vidales ◽

Lluvia C. Cárdenas ◽

Eduardo Robleto ◽

Ronald E. Yasbin ◽

Mario Pedraza-Reyes

Keyword(s):

Oxidative Stress ◽

Bacillus Subtilis ◽

Stationary Phase ◽

Repair System ◽

Induced Mutations ◽

General Role ◽

Error Prevention ◽

Repair Mechanisms ◽

Induced Mutagenesis ◽

Oxidized Guanine

ABSTRACT Previous studies showed that a Bacillus subtilis strain deficient in mismatch repair (MMR; encoded by the mutSL operon) promoted the production of stationary-phase-induced mutations. However, overexpression of the mutSL operon did not completely suppress this process, suggesting that additional DNA repair mechanisms are involved in the generation of stationary-phase-associated mutants in this bacterium. In agreement with this hypothesis, the results presented in this work revealed that starved B. subtilis cells lacking a functional error prevention GO (8-oxo-G) system (composed of YtkD, MutM, and YfhQ) had a dramatic propensity to increase the number of stationary-phase-induced revertants. These results strongly suggest that the occurrence of mutations is exacerbated by reactive oxygen species in nondividing cells of B. subtilis having an inactive GO system. Interestingly, overexpression of the MMR system significantly diminished the accumulation of mutations in cells deficient in the GO repair system during stationary phase. These results suggest that the MMR system plays a general role in correcting base mispairing induced by oxidative stress during stationary phase. Thus, the absence or depression of both the MMR and GO systems contributes to the production of stationary-phase mutants in B. subtilis. In conclusion, our results support the idea that oxidative stress is a mechanism that generates genetic diversity in starved cells of B. subtilis, promoting stationary-phase-induced mutagenesis in this soil microorganism.

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Faculty Opinions recommendation of Alternate day calorie restriction improves clinical findings and reduces markers of oxidative stress and inflammation in overweight adults with moderate asthma.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1071147.524148 ◽

2007 ◽

Author(s):

Andreina Bruno

Keyword(s):

Oxidative Stress ◽

Calorie Restriction ◽

Clinical Findings ◽

Moderate Asthma ◽

Markers Of Oxidative Stress ◽

Overweight Adults

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Biological markers of oxidative stress and allopurinol therapy: A meta-analysis of randomized controlled trials

10.35841/pharmacology.2.1.7-16 ◽

2018 ◽

Vol 02 (01) ◽

Author(s):

Manal M. Alem

Keyword(s):

Oxidative Stress ◽

Randomized Controlled Trials ◽

Biological Markers ◽

Meta Analysis ◽

Controlled Trials ◽

Randomized Controlled ◽

Markers Of Oxidative Stress

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Oxidative status in plasma, urine and saliva of girls with anorexia nervosa and healthy controls: a cross-sectional study

Journal of Eating Disorders ◽

10.1186/s40337-021-00408-6 ◽

2021 ◽

Vol 9 (1) ◽

Author(s):

Alexandra Gaál Kovalčíková ◽

Ľubica Tichá ◽

Katarína Šebeková ◽

Peter Celec ◽

Alžbeta Čagalová ◽

...

Keyword(s):

Oxidative Stress ◽

Anorexia Nervosa ◽

Redox Homeostasis ◽

Oxidative Status ◽

Carbonyl Stress ◽

Healthy Controls ◽

Psychosomatic Disorder ◽

Cross Sectional ◽

Wide Range ◽

Markers Of Oxidative Stress

Abstract Background Anorexia nervosa (AN) is a serious psychosomatic disorder with unclear pathomechanisms. Metabolic dysregulation is associated with disruption of redox homeostasis that might play a pivotal role in the development of AN. The aim of our study was to assess oxidative status and carbonyl stress in plasma, urine and saliva of patients with AN and healthy controls. Methods Plasma, spot urine, and saliva were collected from 111 girls with AN (aged from 10 to 18 years) and from 29 age-matched controls. Markers of oxidative stress and antioxidant status were measured using spectrophotometric and fluorometric methods. Results Plasma advanced oxidation protein products (AOPP) and advanced glycation end products (AGEs) were significantly higher in patients with AN than in healthy controls (by 96, and 82%, respectively). Accordingly, urinary concentrations of AOPP and fructosamines and salivary concentrations of AGEs were higher in girls with AN compared with controls (by 250, and 41% in urine; by 92% in saliva, respectively). Concentrations of thiobarbituric acid reactive substances (TBARS) in saliva were 3-times higher in the patients with AN than in the controls. Overall antioxidants were lower in plasma of girls with AN compared to the controls, as shown by total antioxidant capacity and ratio of reduced and oxidized glutathione (by 43, and 31%, respectively). Conclusions This is the first study assessing wide range of markers of oxidative status in plasma, urine and saliva of the patients with AN. We showed that both, higher levels of markers of oxidative stress and lower antioxidants play a role in redox disruption. Restoration of redox homeostasis might be of the clinical relevance

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Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection

Pharmaceutics ◽

10.3390/pharmaceutics13020271 ◽

2021 ◽

Vol 13 (2) ◽

pp. 271

Author(s):

Lucía Yepes-Molina ◽

José A. Hernández ◽

Micaela Carvajal

Keyword(s):

Oxidative Stress ◽

Heavy Metals ◽

Protective Effect ◽

Uv Radiation ◽

Entrapment Efficiency ◽

Hacat Cells ◽

Biotechnological Applications ◽

Accelerated Stability ◽

The Stability ◽

Brassica Oleracea L

Pomegranate extract (PG-E) has been reported to exert a protective effect on the skin due to its antioxidant activity. Ingredients rich in phenolic compounds are unstable in extract solutions, and, therefore, the use of a suitable nanosystem to encapsulate this type of extract could be necessary in different biotechnological applications. Thus, we investigated the capacity of Brassica oleracea L. (cauliflower) inflorescence vesicles (CI-vesicles) to encapsulate PG-E and determined the stability and the antioxidant capacity of the system over time. In addition, the protective effect against UV radiation and heavy metals in HaCaT cells was also tested. The CI-vesicles had an entrapment efficiency of around 50%, and accelerated stability tests did not show significant changes in the parameters tested. The results for the HaCaT cells showed the non-cytotoxicity of the CI-vesicles containing PG-E and their protection against heavy metals (lead acetate and mercuric chloride) and UV-B radiation through a reduction of oxidative stress. The reduction of the percentage of deleted mtDNA (mtDNA4977, “common deletion”) in UV-treated HaCaT cells due to the presence of CI-vesicles containing PG-E indicated the mechanism of protection. Therefore, the effects of CI-vesicles loaded with PG-E against oxidative stress support their utilization as natural cosmeceuticals to protect skin health against external damage from environmental pollution and UV radiation.

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Effect of Tai Chi on Markers of Oxidative Stress: Systematic Review and Meta-Analysis

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18073458 ◽

2021 ◽

Vol 18 (7) ◽

pp. 3458

Author(s):

Juana Rosado-Pérez ◽

Osvaldo D. Castelán-Martínez ◽

Abril J. Mújica-Calderón ◽

Martha A. Sánchez-Rodríguez ◽

Víctor Manuel Mendoza-Núñez

Keyword(s):

Oxidative Stress ◽

Systematic Review ◽

Superoxide Dismutase ◽

Sedentary Behavior ◽

Tai Chi ◽

Meta Analysis ◽

Cochrane Library ◽

Stress Markers ◽

Markers Of Oxidative Stress ◽

Mean Differences

Background: This study aimed to synthesize the evidence of the effect of practicing Tai Chi on oxidative stress markers (OxSM). Methods: This systematic review and meta-analysis was conducting using the MEDLINE, Cochrane Library, ScienceDirect, Scopus, Epistemonikos, Lilacs, and Ovid databases to identify randomized (RCT) and non-randomized (NRCT) clinical trials that evaluated the Tai Chi effect on OxSM compared to sedentary behavior, walking or yoga. Pooled mean differences (MDs) with 95% confidence intervals (95%CI) were estimated using the inverse variance method to determine the effect of Tai Chi on OxSM. PROSPERO register: CRD42019138362. Results: Five RCT and five NRCT were included. Compared to sedentary behavior, regular Tai Chi practice increases the levels of the enzymes superoxide dismutase (MD = 34.97 U/mL, (95%CI, 9.45 to 60.48), 344 participants) and catalase (MD = 15.63 U/mL, (95%CI, 4.05 to 27.22), 110 participants), as well as reducing the levels of lipoperoxides (MD = −0.02 µmol/L, (95%CI, −0.04 to −0.00), 234 participants). For comparisons with walking or yoga, only one study per activity was identified comparing the effect on OxSM. Conclusions: Regular Tai Chi practice increases the levels of superoxide dismutase and catalase, as well as reducing the levels of lipoperoxides. More studies are necessary to determine the effect of Tai Chi on OxSM when compared to other physical activities.

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