scholarly journals Histological Ex Vivo Evaluation of Peri-Incisional Thermal Effect Created by a New-Generation CO2Superpulsed Laser

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
G. Palaia ◽  
A. Del Vecchio ◽  
A. Impellizzeri ◽  
G. Tenore ◽  
P. Visca ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Connective Tissue ◽  
Thermal Damage ◽  
Histological Analysis ◽  
Continuous Wave ◽  
Ex Vivo ◽  
Optical Microscope ◽  
Control Group ◽  
New Generation ◽  
Ex Vivo Study

The purpose of this study is the evaluation of the histological effects of a new-generation superpulsed CO2laser through an “ex vivo” study. A CO2(λ = 10,600 nm) ultra-speed laser (SmartUS20D, DEKA, Florence, Italy) has been used at different parameters from 2 to 4 watt in Continuous Wave (CW) and Pulsed Wave (PW, 50 Hz) to obtain 30 samples from pig cadaver tongues. All the specimens have been subdivided into 6 groups (from A to F) and each group consisted of 5 samples. A final specimen has been taken by scalpel and used as control group. Histological analysis has been performed using an optical microscope (Leica DM 2000) at a magnification of ×40. Results showed that histological readability was optimal in all the samples. The thermal damage has been negligible in all the groups. Furthermore, the average of thermal damage was 0,095 mm in the epithelial, while it was 0.245 mm in the connective tissue. Statistical analysis using Graphpad Prism 5 software showed no significant differences among the groups. CO2laser demonstrated a good surgical effectiveness provoking little peripheral damage onto the cut edges and allowing a safe histological diagnosis.

scholarly journals Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue Biopsies

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Gaspare Palaia ◽  
Federico Renzi ◽  
Daniele Pergolini ◽  
Alessandro Del Vecchio ◽  
Paolo Visca ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Soft Tissue ◽  
Diode Laser ◽  
Thermal Damage ◽  
Continuous Wave ◽  
Ex Vivo ◽  
Histological Evaluation ◽  
Epithelial Tissue ◽  
Medical Laser ◽  
Oral Soft Tissue

Introduction. Laser-induced thermal effects can preclude a safe histological evaluation of biopsy resection margins. The aim of this study was to evaluate the suitability of a 976 nm diode laser in oral soft tissue biopsies in an ex vivo study. Materials and Methods. A 976 nm diode laser (Solase®, Lazon Medical Laser, China) has been used in the contact mode, using a 400 μm fiber tip, at different parameters from 4 to 6 W in the continuous wave (CW), with a fluence between 3184 and 4777 J/cm2, and pulsed wave (PW) mode, with a fluence between 3184 and 4777 J/cm2, to obtain 30 samples from fresh pig cadaver tongues. All specimens were subdivided into 6 groups (from A to F), and each group consisted of 5 samples. Two sections were obtained from each sample. A histological analysis was performed using an optical microscope at magnifications of 5x and 10x. Statistical analysis was carried out using Kruskal–Wallis and Dunn’s tests. Results. The results showed that histological readability was optimal in all the samples. The thermal damage was negligible in all groups. The average thermal damage was 208.40 ± 133.81 μm in the epithelial tissue and 330.14 ± 147.45 μm in the connective tissue. The statistical analysis showed no differences between the groups ( p  > 0.05). Conclusion. A 976 nm diode laser demonstrated good surgical effectiveness that provoked little peripheral damage in the cut edges and allowed a safe histological diagnosis. Clinical Relevance. In oral pathology, many times, there is fear in using the laser to remove some lesions due to its thermal effect on the tissues close to the lesion. This effect is always present in the use of the laser, but the intent is to minimize this effect to have as little alteration as possible on the surrounding tissues.

scholarly journals Experimental justification for prompt neutralization of traumatic action of thermal exo- and endogenous damage factors (morphological features)

2021 ◽  
Vol 26 (6) ◽  
pp. 3040-3046
Author(s):  
VASYL NAGAICHUK ◽  
ROMAN CHORNOPYSHCHUK ◽  
OLEKSANDR NAZARCHUK ◽  
LUDMILA SIDORENKO ◽  
MYKOLA ZHELIBA ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Histological Examination ◽  
Thermal Damage ◽  
Burn Injuries ◽  
Control Group ◽  
Experimental Conditions ◽  
Skin Appendages ◽  
Experimental Justification ◽  
Traumatic Effect ◽  
Burn Depth

Objective: morphological substantiation of efficiency of prompt neutralization of traumatic action of exogenous and endogenous damage factors in burn injuries in experimental conditions. After simulating the burns on 60 rats, the traumatic hyperthermic damage factors in the main group were immediately neutralized by a gauze napkin soaked in water. Depending on its duration, the animals were divided into subgroups. Such applications were not performed on the control group. The study involved histological examination of tissues. Animals of the main groups had the presence of histologically confirmed skin appendages with a formed scar of connective tissue without signs of inflammation. In animals of the control group there were no skin appendages, which indicates a deeper thermal damage to tissues and the impossibility of self-epithelialization of wounds. These results confirm the importance and necessity of prompt neutralization of the traumatic effect of damage factors as the main elements of burn depth formation.

Effects of Dall-Miles Cables on the Cement Mantle in Total Hip Arthroplasty: A Cadaveric Study

2003 ◽  
Vol 13 (1) ◽  
pp. 29-31
Author(s):  
R. Kingston ◽  
J. Noël ◽  
B. Murphy ◽  
T. C Lee ◽  
M.G. Walsh
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Hip Arthroplasty ◽  
Ex Vivo ◽  
Cement Mantle ◽  
Control Group ◽  
Before And After ◽  
Diamond Saw ◽  
Ex Vivo Study ◽  
Scanning Electron

Dall-Miles cables are widely used for trochanteric re-attachment in hip arthroplasty, but their effects on the cement mantle have not been reported. We have carried out an ex vivo study to investigate the influence of cables on the mantle. Charnley femoral components were implanted in eight proximal human femora using Palacos cement. Wires (control group, n=4) or 2mm stainless steel Dall-Miles cables (n=4) were passed through holes drilled in the proximal femur before the cement was introduced. The wires or cables were tightened using the appropriate instruments after the cement had hardened. The specimens were sectioned using a diamond saw, and examined by scanning electron microscopy and light microscopy before and after staining with penetrant dye. Deformation of the strands at the tension edge of each cable, with debonding from the cement in two specimens, was observed. There was no damage to cement at the compression edge. Tensioning of Dall-Miles cables passing through Palacos cement does not appear to damage the mantle.

scholarly journals Effect of Toothpastes with Different Abrasives on Eroded Human Enamel: An in situ/ex vivo Study

2013 ◽  
Vol 7 (1) ◽  
pp. 132-139 ◽  
Author(s):  
Meire Coelho Ferreira ◽  
Maria Letícia Ramos-Jorge ◽  
Alberto Carlos Botazzo Delbem ◽  
Ricardo de Sousa Vieirac
Keyword(s):  
Ex Vivo ◽  
Soft Drink ◽  
Dental Enamel ◽  
Additional Treatment ◽  
Control Group ◽  
Wear Depth ◽  
Human Enamel ◽  
Waiting Periods ◽  
Ex Vivo Study

The aim of the present study was to investigate the abrasive effect of CaCO3 and SiO2-based fluoride-free experimental toothpastes on eroded human permanent dental enamel and evaluate the effectiveness of waiting periods between acid exposure and tooth brushing. Twelve volunteers wore palatal appliances containing human enamel blocks for two periods of five days each. The appliances were immersed in a soft drink for five minutes four times a day (9:00 am, 11:00 am, 2:00 pm and 4:00 pm). On two occasions, two blocks were not submitted to additional treatment; two blocks were brushed (30 s) either with a CaCO3 or SiO2 toothpaste immediately after erosion and two blocks were brushed 1 h after erosion. Thus, the sample was divided into six groups: erosion alone (CaCO3 and SiO2 control); brushing with fluoride-free toothpaste (CaCO3 immediate and 1 h after erosion; SiO2 immediate and 1 h after erosion). Significant differences in wear depth were found between the enamel blocks in the CaCO3 immediate and 1 h after erosion groups and the blocks in the CaCO3 control group (p=0.001; p=0.022). No significant differences were found regarding the change in roughness and wear depth between blocks submitted to immediate abrasion and 1 h after erosion (CaCO3 and SiO2). The data revealed that surface roughness and wear depth is increased when erosion is combined with dental abrasion, regardless of the abrasive used. Waiting for 1 h to brush the eroded blocks offered no protective effect.

scholarly journals Effect of Smear Layer on the Apical Seal of Endodontically Treated Teeth: An ex vivo Study

2012 ◽  
Vol 13 (1) ◽  
pp. 23-26 ◽  
Author(s):  
KG Nischith ◽  
GPV Srikumar ◽  
Shuaib Razvi ◽  
R Vinay Chandra
Keyword(s):  
Root Canal ◽  
Ex Vivo ◽  
Smear Layer ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Root Canals ◽  
Group A ◽  
Group B ◽  
Root Canal Irrigant ◽  
Ex Vivo Study

ABSTRACT Aim The purpose of this study was to evaluate the effect of smear layer on apical seal of endodontically treated teeth. Materials and methods Sixty freshly extracted human maxillary central incisor teeth were selected and were randomly divided into two experimental groups. Group A of 25 teeth and group B of 25 teeth and a control group of 10 teeth. Cleaning and shaping of the root canals were performed using endodontic K-files up to no. 50 size file in step-back technique. During the process, in root canals of group A, 17% EDTA (ethylenediaminetetraacetic acid) followed by 3% NaOCl (sodium hypochlorite) was used as root canal irrigant. In group B, MTAD (mixture tetracycline citric acid and detergent) was used as a root canal irrigant. In control group, saline was used as root canal irrigant. The root surfaces were then coated with nail polish of both experimental groups and control group. The samples were then immersed in India ink dye for 1 week at 37°C. Results The statistical analysis of the results showed that the apical leakage was significantly more in teeth, where smear layer was not removed. Conclusion The removal of smear layer improves the longterm apical seal and success of endodontically treated teeth. Clinical significance The development and maintenance of apical seal is desirable and considered to be a major prerequisite to improve the outcome of root canal treatment. How to cite this article Nischith KG, Srikumar GPV, Razvi S, Chandra RV. Effect of Smear Layer on the Apical Seal of Endodontically Treated Teeth: An ex vivo Study. J Contemp Dent Pract 2012;13(1):23-26.

Comparison of heat induced damage at the saphenofemoral junction after ablation with 1,470 nm laser or radiofrequency

VASA ◽  
2017 ◽  
Vol 46 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Evren Ozcinar ◽  
Mehmet Cakici ◽  
Oktay Korun ◽  
Unsal Han ◽  
Ugursay Kiziltepe
Keyword(s):  
Laser Ablation ◽  
Continuous Wave ◽  
Ex Vivo ◽  
In Vivo Studies ◽  
Control Group ◽  
Damage Score ◽  
Significant Difference ◽  
Level 1 ◽  
Total Damage ◽  
Saphenofemoral Junction

Abstract. Background: The aim of this study was to evaluate the heat induced damage at the saphenofemoral junction level according to histopathological changes after radiofrequency or 1,470 nm radial tip laser ablation. Patients and methods: Varicose vein segments of 6–10 mm in diameter were exposed to radiofrequency (Closure Fast catheter, 7 cm heat segment, one cycle, 15 seconds, 10 Watt, 120 °C) or laser ablation (1,470 nm radial tip, continuous wave, vein diameter: 6 cm/8 cm/10 cm-power: 10 Watt-pullback speed: 2.2 mm/s, 1.7 mm/s, 1.3 mm/s-LEED: 45J/cm, 60J/cm, 75J/cm-EFE 25J/cm2, respectively). Approximate 2 cm segments of the vein were left untreated, then histopathological examinations of the untouched segments (5 slices: level 1 – furthest segment, level 2 – nearest segment) for heat induced damage were performed. A total damage scoring system was established, including the presence of endothelial swelling, intimal thickening, cellular vacuolisation in the muscle layer, oedema in the tunica media, and extent of necrosis. Results: At level 1, the furthest segment of the specimen, there was no significant difference between the laser and control group, while the total damage score of the radiofrequency group was significantly higher than the control group (p < 0.01). Radiofrequency group had higher total damage score compared to the laser group at level 1 (p < 0.01), 2 (p < 0.01), and 5 (p < 0.01); while no significant difference was observed at level 3 (p = 0.46) and 4 (p = 0.13). Conclusions: Significant heat induced damage may be seen even if the 2 cm segment of the vessel is left unablated. Radiofrequency ablation seems to cause more histological damage than laser ablation in this ex vivo study. Further in vivo studies are necessary, in order to validate these findings.

scholarly journals Assessment of Temperature Rise and Time of Alveolar Ridge Splitting by Means of Er:YAG Laser, Piezosurgery, and Surgical Saw: An Ex Vivo Study

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Jacek Matys ◽  
Rafał Flieger ◽  
Marzena Dominiak
Keyword(s):  
Temperature Rise ◽  
Laser Power ◽  
Thermal Damage ◽  
Er:Yag Laser ◽  
Ex Vivo ◽  
Common Adverse Effect ◽  
Study Groups ◽  
Alveolar Ridge ◽  
Bone Surface ◽  
Ex Vivo Study

The most common adverse effect after bone cutting is a thermal damage. The aim of our study was to evaluate the bone temperature rise during an alveolar ridge splitting, rating the time needed to perform this procedure and the time to raise the temperature of a bone by 10°C, as well as to evaluate the bone carbonization occurrence. The research included 60 mandibles (n=60) of adult pigs, divided into 4 groups (n=15). Two vertical and one horizontal cut have been done in an alveolar ridge using Er:YAG laser with set power of 200 mJ (G1), 400 mJ (G2), piezosurgery unit (G3), and a saw (G4). The temperature was measured by K-type thermocouple. The highest temperature gradient was noted for piezosurgery on the buccal and lingual side of mandible. The temperature rises on the bone surface along with the increase of laser power. The lower time needed to perform ridge splitting was measured for a saw, piezosurgery, and Er:YAG laser with power of 400 mJ and 200 mJ, respectively. The temperature rise measured on the bone over 10°C and bone carbonization occurrence was not reported in all study groups. Piezosurgery, Er:YAG laser (200 mJ and 400 mJ), and surgical saw are useful and safe tools in ridge splitting surgery.

New generation argon plasma coagulation in flexible endoscopy: Ex vivo study and clinical experience

2006 ◽  
Vol 21 (7) ◽  
pp. 1122-1128 ◽  
Author(s):  
KAZUKI SUMIYAMA ◽  
MITSURU KAISE ◽  
MASAYUKI KATO ◽  
SYOUICHI SAITO ◽  
KENICHI GODA ◽  
...  
Keyword(s):  
Clinical Experience ◽  
Ex Vivo ◽  
Argon Plasma Coagulation ◽  
Argon Plasma ◽  
Flexible Endoscopy ◽  
Plasma Coagulation ◽  
New Generation ◽  
Ex Vivo Study

scholarly journals Acetabular ventroversion using the sacroiliac wedge, with or without pelvic osteotomies in dogs: an ex vivo study

2019 ◽  
Vol 39 (8) ◽  
pp. 643-648
Author(s):  
Fernanda M. Ikenaga ◽  
Jessé R. Rocha ◽  
Leonardo L. Carvalho ◽  
Cristiane S. Honsho ◽  
Fernanda G.G. Dias ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Surgical Techniques ◽  
Pelvic Osteotomy ◽  
Axial Rotation ◽  
Control Group ◽  
Acetabular Coverage ◽  
Joint Stability ◽  
Canine Hip Dysplasia ◽  
Ex Vivo Study ◽  
Wedge Technique

ABSTRACT: Canine hip dysplasia (CHD) is a common condition observed in the surgical clinics for small animals. Among the surgical techniques for management of CHD, triple pelvic osteotomy and sacroiliac wedge promote acetabular lateral axial rotation (ventroversion), increasing acetabular coverage and joint stability. The present study aimed to evaluate radiographically, by measuring the Norberg angle (NA) and the acetabular coverage percentage (ACP), the acetabular ventroversion induced by the sacroiliac wedge technique, with or without pelvic osteotomies; we also checked the feasibility of wedges made of polyamide with an angulation of 20° and 30°. The software used to measure NA and ACP was AutoCAD® 2009. Pelves from 10 canine corpses were evaluated radiographically at four time-points: M0 (Control Group), M1 (wedges of 20° and 30°), M2 and M3 (wedges associated with bilateral pubis and ischium osteotomies, respectively). There was no significant increase in the acetabular ventroversion at M1, M2, and M3. The polyamide sacroiliac wedge technique proved to be feasible, stable, and easy to apply. Further, the software proved to be efficient and easy to use for NA and ACP measurements. In the present study, even in the cases of non-dysplasic adult canine corpses, it was concluded that the sacroiliac wedge technique does not require to be accompanied by pubis and ischial osteotomies because they did not significantly increase the NA and ACP.

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