Progression of Gene Expression Changes following a Mechanical Injury to Articular Cartilage as a Model of Early Stage Osteoarthritis

An impact injury model of early stage osteoarthritis (OA) progression was developed using a mechanical insult to an articular cartilage surface to evaluate differential gene expression changes over time and treatment. Porcine patellae with intact cartilage surfaces were randomized to one of three treatments: nonimpacted control, axial impaction (2000 N), or a shear impaction (500 N axial, with tangential displacement to induce shear forces). After impact, the patellae were returned to culture for 0, 3, 7, or 14 days. At the appropriate time point, RNA was extracted from full-thickness cartilage slices at the impact site. Quantitative real-time PCR was used to evaluate differential gene expression for 18 OA related genes from four categories: cartilage matrix, degradative enzymes and inhibitors, inflammatory response and signaling, and cell apoptosis. The shear impacted specimens were compared to the axial impacted specimens and showed that shear specimens more highly expressed type I collagen (Col1a1) at the early time points. In addition, there was generally elevated expression of degradative enzymes, inflammatory response genes, and apoptosis markers at the early time points. These changes suggest that the more physiologically relevant shear loading may initially be more damaging to the cartilage and induces more repair efforts after loading.

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Differential gene expression profiling of gastric intraepithelial neoplasia and early-stage adenocarcinoma

World Journal of Gastroenterology ◽

10.3748/wjg.v20.i47.17883 ◽

2014 ◽

Vol 20 (47) ◽

pp. 17883-17893 ◽

Cited By ~ 5

Author(s):

Xue Xu ◽

Lin Feng ◽

Yu Liu ◽

Wei-Xun Zhou ◽

Ying-Cai Ma ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Differential Gene Expression ◽

Expression Profiling ◽

Early Stage ◽

Intraepithelial Neoplasia ◽

Differential Gene

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Differential gene expression in articular cartilage between rheumatoid arthritis and endemic Kashin–Beck disease

Bioscience Reports ◽

10.1042/bsr20190188 ◽

2019 ◽

Vol 39 (6) ◽

Cited By ~ 1

Author(s):

Zongqiang Gao ◽

Chen Duan ◽

Fang-fang Yu ◽

Xiong Guo

Keyword(s):

Rheumatoid Arthritis ◽

Gene Expression ◽

Articular Cartilage ◽

Signaling Pathway ◽

Differential Gene Expression ◽

Mapk Signaling ◽

Janus Kinase ◽

Ppi Network ◽

Differential Gene ◽

Beck Disease

AbstractKashin–beck disease (KBD) is endemic chronic osteoarthrosis and its pathogenesis is still unclear. The present study aimed to explore differential gene expression in articular cartilage between patients with rheumatoid arthritis (RA) and KBD. Articular cartilages were collected from KBD and RA patients, and differentially expressed genes (DEGs) were analyzed by RNA-seq. The signaling pathway and biological process (BP) of the DEGs were identified by enrichment analysis. The protein–protein interaction (PPI) network of DEGs and the key genes of KBD were identified by network analysis with STRING and cytoscape software. We identified 167 immune-related DEGs in articular cartilage samples from KBD patients compared with RA. The up-regulation of MAPK signaling pathway and the down-regulation of signaling pathways such as toll-like receptor, janus kinase-signal transducers and activators of transcription, leukocyte migration, T-cell receptor and chemokine, and antigen processing and presentation were involved in KBD. We identified 137 genes nodes related with immune and mapped the PPI network diagram. BP analysis revealed that immune response, calcium ion homeostasis, blood vessel morphogenesis, inflammatory response, lymphocyte proliferation, and MAPK activation were involved in KBD. In conclusion, gene expression profiling can be used to identify the different mechanism of pathogenesis between KBD and RA.

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Differential Gene Expression of the Intermediate and Outer Interzone Layers of Developing Articular Cartilage in Murine Embryos

Stem Cells and Development ◽

10.1089/scd.2013.0235 ◽

2014 ◽

Vol 23 (16) ◽

pp. 1883-1898 ◽

Cited By ~ 24

Author(s):

Florien Jenner ◽

Arne IJpma ◽

Mairead Cleary ◽

Daphne Heijsman ◽

Roberto Narcisi ◽

...

Keyword(s):

Gene Expression ◽

Articular Cartilage ◽

Differential Gene Expression ◽

Differential Gene ◽

Murine Embryos

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Reprogramming of the Wheat Transcriptome in Response to Infection with Claviceps Purpurea, the Causal Agent of Ergot

10.21203/rs.3.rs-126182/v1 ◽

2020 ◽

Author(s):

Lesley A. Boyd ◽

Eleni Tente ◽

Nelzo Ereful ◽

Anyela Camargo Rodriguez ◽

Paul Grant ◽

...

Keyword(s):

Gene Expression ◽

Differential Gene Expression ◽

Early Stage ◽

Receptor Protein ◽

Claviceps Purpurea ◽

Long Distance ◽

Transmitting Tissue ◽

Significant Differential Expression ◽

Differential Gene ◽

Wheat Transcriptome

Abstract Background: Ergot, caused by the fungal pathogen Claviceps purpurea, infects the female flowers of a range of cereal crops, including wheat. To understand the interaction between C. purpurea and hexaploid wheat we undertook an extensive examination of the reprogramming of the wheat transcriptome in response to C. purpurea infection through floral tissues (i.e. the stigma, transmitting and base ovule tissues of the ovary) and over time. Results: C. purpurea hyphae were observed to have grown into and down the stigma at 24 hours (H) after inoculation. By 48H hyphae had grown through the transmitting tissue into the base, while by 72H hyphae had surrounded the ovule. By 5 days (D) the ovule had been replaced by fungal tissue. Significant differential gene expression was first observed at 1H in the stigma tissue. Many of the wheat genes differentially transcribed in response to C. purpurea infection were associated with plant hormones and included the ethylene (ET), auxin, cytokinin, gibberellic acid (GA), salicylic acid and jasmonic acid (JA) biosynthetic and signaling pathways. Hormone-associated genes were first detected in the stigma and base tissues at 24H, but not in the transmitting tissue. Genes associated with GA and JA pathways were seen in the stigma at 24H, while JA and ET-associated genes were identified in the base at 24H. In addition, several defence-associated genes were differential expressed in response to C. purpurea infection, including antifungal proteins, endocytosis/exocytosis-related proteins, NBS-LRR class proteins, genes involved in programmed cell death, receptor protein kinases and transcription factors. Of particular interest was the identification of significant differential expression of wheat genes in the base tissue well before the appearance of fungal hyphae, suggesting that a mobile signal, either pathogen or plant-derived, is delivered to the base prior to colonisation.Conclusions: Multiple host hormonal biosynthesis and signalling pathways were significantly perturbed from an early stage in the wheat – C. purpurea interaction. Significant differential gene expression at the base of the ovary, ahead of arrival of the pathogen, indicated the potential presence of a long-distance signal modifying host gene expression.

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