Bronchoalveolar Lavage Fluid IFN-γ+Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis

In sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 cells producing IFN-γhave been observed in peripheral blood. The balance between Th1, Th17, and FoxP3+CD4+T cell subsets in sarcoidosis remains unclear. Bronchoalveolar lavage fluid cells, from 30 patients with sarcoidosis, 18 patients with other diffuse parenchymal lung diseases, and 15 healthy controls, were investigated with flow cytometry for intracellular expression of FoxP3. In a subset of the patients, expression of the cytokines IL17A and IFN-γwas investigated. The fractions of FoxP3+CD4+T cells and Th17 cells were both lower in sarcoidosis compared to controls (P=0.017andP=0.011, resp.). The proportion of Th17 cells positive for IFN-γwas greater in sarcoidosis than controls (median 72.4% versus 31%,P=0.0005) and increased with radiologic stage (N=23,rho=0.45, andP=0.03). IFN-γ+Th17 cells were highly correlated with Th1 cells (N=23,rho=0.64, andP=0.001), and the ratio of IFN-γ+Th17/FoxP3+CD4+T cells was prominently increased in sarcoidosis. IFN-γ+Th17 cells may represent a pathogenic subset of Th17 cells, yet their expression of IFN-γcould be a consequence of a Th1-polarized cytokine milieu. Our results indicate a possible immune cell imbalance in sarcoidosis.

Download Full-text

Yupingfeng Pulvis Regulates the Balance of T Cell Subsets in Asthma Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/6916353 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Zhigang Wang ◽

Xueting Cai ◽

Zhonghua Pang ◽

Dawei Wang ◽

Juan Ye ◽

...

Keyword(s):

Bronchoalveolar Lavage ◽

Th17 Cells ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Treg Cells ◽

T Cell Subsets ◽

Control Group ◽

Group Model ◽

Therapy Effect ◽

Tract Infections

Background. Yupingfeng Pulvis (HFBP) had played an active role in many diseases, especially respiratory tract infections. Exploring the possible prevention mechanism of HFBP may provide new ideas in clinical applications for this well-known herbal formula.Purpose. To study the possible mechanisms of therapy effect of HFBP on asthma mice via regulating the balance of Tregs and Th17 cells.Method. The female BALB/c mice were divided into five groups: control group, model group, prednisone (5.5 mg/kg) group, and 22 g/kg HFBP and 44 g/kg HFBP groups. Ovalbumin was used to make the asthma model of mice; the drug was ig administered daily after atomization for consecutive 15 d. The mice were killed after the last administration. The paraffin-embedded tissue sections of the lungs were stained by H&E. Tregs and Th17 cells in bronchoalveolar lavage fluid were detected by flow cytometry. IL-4, TGF-β, and TNF-αin the serum were detected by ELISA assay.Results. HFBP could alleviate the inflammation in the lung tissue of mice, decrease the proportion of Th17 cells, and increase the proportion of Treg cells in bronchoalveolar lavage fluid. HFBP could decrease IL-4 and TNF-αlevel and increase TGF-βlevel in blood.Conclusion. HFBP could treat the asthma through impacting the balance of Th17 cells and Treg cells as well as the levels of related inflammatory cytokines in asthma mice.

Download Full-text

Imaging Changes and Immune-Checkpoint Expression on T Cells in Bronchoalveolar Lavage Fluid from Patients with Pulmonary Sarcoidosis

Biomedicines ◽

10.3390/biomedicines9091231 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1231

Author(s):

Yasuaki Kotetsu ◽

Toyoshi Yanagihara ◽

Kunihiro Suzuki ◽

Hiroyuki Ando ◽

Daisuke Eto ◽

...

Keyword(s):

T Cells ◽

Bronchoalveolar Lavage ◽

Immune Checkpoint ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Pulmonary Sarcoidosis ◽

Immune Checkpoint Molecules ◽

Ct Findings ◽

Programmed Cell Death 1 ◽

Spontaneous Improvement

Sarcoidosis is a systemic, granulomatous disease caused by unknown immunological abnormalities. The organs most vulnerable to sarcoidosis are the lungs. Patients often resolve spontaneously, but the lungs can also be severely affected. Although details regarding prognostic factors in sarcoidosis patients with lung involvement remain unclear, several reports have suggested that immune checkpoint molecules are involved in the pathogenesis of sarcoidosis. In this study, we divided sarcoidosis patients into two groups based on chest computed tomography (CT) findings and compared immune checkpoint molecules expressed on T cells in bronchoalveolar lavage fluid (BALF) in the two groups, using flow cytometry. We found elevated programmed cell death 1 (PD-1) or T cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3) expression on T cells in BALF in patients with spontaneous improvement in CT findings, compared with those in patients without improvement in CT findings. In conclusion, our study implies that PD-1 or TIM-3 expression on T cells in BALF may be a prognostic factor for pulmonary lesions in sarcoidosis.

Download Full-text

P2‐22: Imaging changes and immune‐checkpoint expression on T cells in bronchoalveolar lavage fluid from patients with pulmonary sarcoidosis

Respirology ◽

10.1111/resp.14150_118 ◽

2021 ◽

Vol 26 (S3) ◽

pp. 122-122

Keyword(s):

T Cells ◽

Bronchoalveolar Lavage ◽

Immune Checkpoint ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Pulmonary Sarcoidosis

Download Full-text

Adipose-Derived Stem Cells Ameliorate Allergic Airway Inflammation by Inducing Regulatory T Cells in a Mouse Model of Asthma

Mediators of Inflammation ◽

10.1155/2014/436476 ◽

2014 ◽

Vol 2014 ◽

pp. 1-12 ◽

Cited By ~ 34

Author(s):

Kyu-Sup Cho ◽

Mi-Kyung Park ◽

Shin-Ae Kang ◽

Hee-Young Park ◽

Sung-Lyong Hong ◽

...

Keyword(s):

Stem Cells ◽

T Cells ◽

Regulatory T Cells ◽

Bronchoalveolar Lavage ◽

Airway Inflammation ◽

Bronchoalveolar Lavage Fluid ◽

Immune Cell ◽

Allergic Airway Inflammation ◽

Lavage Fluid ◽

Th2 Cytokines

Although several studies have demonstrated that mesenchymal stem cells derived from adipose tissue (ASCs) can ameliorate allergic airway inflammation, the immunomodulatory mechanism of ASCs remains unclear. In this study, we investigated whether regulatory T cells (Tregs) induction is a potential mechanism in immunomodulatory effects of ASCs on allergic airway disease and how these induced Tregs orchestrate allergic inflammation. Intravenous administration of ASCs significantly reduced allergic symptoms and inhibited eosinophilic inflammation. Airway hyperresponsiveness, total immune cell and eosinophils in the bronchoalveolar lavage fluid, mucus production, and serum allergen-specific IgE and IgG1 were significantly reduced after ASCs administration. ASCs significantly inhibited Th2 cytokines (IL-4, IL-5, and IL-13) and enhanced Th1 cytokine (IFN-γ) and regulatory cytokines (IL-10 and TGF-β) in the bronchoalveolar lavage fluid and lung draining lymph nodes. Furthermore, levels of IDO, TGF-β, and PGE2were significantly increased after ASCs administration. Interestingly, this upregulation was accompanied by increased Treg populations. In conclusion, ASCs ameliorated allergic airway inflammation and improved lung function through the induction of Treg expansion. The induction of Treg by ASCs involves the secretion of soluble factors such as IDO, TGF-β, and PGE2and Treg might be involved in the downregulation of Th2 cytokines and upregulation of Th1 cytokines production.

Download Full-text