Quantitative Analysis of Resorcinol from Marketed Hair Tonic Using Liquid Chromatographic Technique

Quantitative estimation of resorcinol from marketed pharmaceutical formulation has been reported in this study. Resorcinol as a pharmaceutical ingredient has a broad spectrum of application but its application is limited due to its toxic side effects. Method for the accurate estimation of resorcinol is therefore essential. In the current study we have developed a chromatographic technique for its estimation from a marketed hair tonic meant for the treatment of several dermatological diseases of the scalp. A stainless steel column 25 cm in length and 4 mm internal diameter packed with octadecylsilane (5 µm) was used for this purpose. The mobile phase was a mixture of phosphate buffer of pH 2.8 and acetonitrile. The flow rate was 0.6 mL·min−1 and the detection wavelength was 280 nm. The method was found to be linear between concentration range 10.28 µg·mL−1 to 71.96 µg·mL−1 with r2 value 0.999. The accuracy of the method and the intraday and interday precession study presents the applicability of the method for the estimation of resorcinol from any pharmaceutical and cosmetic product containing resorcinol.

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Gas-Liquid Chromatographic Determination of Chloroform in Toothpastes, Using the Headspace Analytical Technique

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/57.3.560 ◽

1974 ◽

Vol 57 (3) ◽

pp. 560-562

Author(s):

Martin J Stutsman

Keyword(s):

Stainless Steel ◽

Liquid Chromatography ◽

Chromatographic Determination ◽

Gas Liquid Chromatography ◽

Analytical Technique ◽

Steel Column ◽

Stainless Steel Column ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract Chloroform in toothpastes is determined by gas-liquid chromatography of the vapors in the headspace over a diluted sample, using an electron capture detector. The method is sensitive to small amounts of chloroform and avoids the injection of materials deleterious to the column. The 12' × 1/8" od stainless steel column is packed with acid-washed Gas-Chrom R coated with 2 0% Carbowax 20M. Recoveries of chloroform added to toothpastes range from 95 to 104%.

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Multiresidue Liquid Chromatographic Method for Simultaneous Determination of Pyrethroid Insecticides in Fruits and Vegetables

Journal of AOAC International ◽

10.1093/jaoac/78.6.1474 ◽

1995 ◽

Vol 78 (6) ◽

pp. 1474-1480 ◽

Cited By ~ 8

Author(s):

Guo-Fang Pang ◽

Yan-Zhong Chao ◽

Xie-Shan Liu ◽

Chun-Lin Fan

Keyword(s):

Simultaneous Determination ◽

Mobile Phase ◽

Fruits And Vegetables ◽

Deionized Water ◽

Pyrethroid Insecticides ◽

Liquid Chromatographic Method ◽

Steel Column ◽

Stainless Steel Column ◽

Liquid Chromatographic

Abstract A simple and rapid liquid chromatographic (LC) method has been developed for simultaneous determination of 9 pyrethroid insecticides (biphenthrin, cypermethrin, fenpropathrin, fenvalerate, flucythrinate, methothrin, permethrin, py-115, and tetramethrin) in fruits and vegetables. Residues are extracted from crops with methanol and partitioned with toluene. Extracts are cleaned up by Florisil–charcoal column chromatography. LC separation is performed on a μBondapak C18 stainless steel column with acetonitrile–deionized water as mobile phase. The insecticides are detected at 206 nm with 0.03 absorbance unit full scale. Recoveries of 9 pyrethroid insecticides from 6 crops (cucumbers, tomatoes, cabbages, apples, pears, and peaches) fortified at 0.5–5.0 mg/kg were 62.7–129.2%. Detection limits were about 0.05 mg/kg, except for py-115, for which detection limit was 0.10 mg/kg.

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High Pressure Liquid Chromatographic Determination of Pentachlorophenol by Using Paired Ion Chromatography Reagents

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/62.5.1004 ◽

1979 ◽

Vol 62 (5) ◽

pp. 1004-1006

Author(s):

Elmer H Hayes

Keyword(s):

High Pressure ◽

Mobile Phase ◽

Chromatographic Determination ◽

Hplc Method ◽

High Pressure Liquid Chromatographic ◽

Steel Column ◽

Stainless Steel Column ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract A high pressure liquid chromatographic (HPLC) method for determining pentachlorophenol in formulations is described. Samples containing pentachlorophenol are accurately weighed in suitable volumetric flasks and diluted with dioxane. The sample is then injected onto a stainless steel column containing μBondapak C18. The mobile phase is 60% methanol/PIC A and 40% water/PIC A. This method is simple and eliminates many of the extractions required in other methods of analysis.

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Experimental Investigation Into Creep Buckling of a Stainless Steel Plate Column Under Axial Compression at Extremely High Temperatures

Journal of Pressure Vessel Technology ◽

10.1115/1.4033155 ◽

2016 ◽

Vol 139 (1) ◽

Cited By ~ 1

Author(s):

Byeongnam Jo ◽

Koji Okamoto

Keyword(s):

Stainless Steel ◽

High Temperatures ◽

High Speed ◽

Failure Time ◽

Empirical Correlation ◽

Buckling Behavior ◽

Creep Buckling ◽

Steel Column ◽

Stainless Steel Column ◽

Buckling Failure

This study aims to investigate the creep buckling behavior of a stainless steel column under axial compressive loading at extremely high temperatures. Creep buckling failure time of a slender column with a rectangular cross section was experimentally measured under three different temperature conditions, namely, 800, 900, and 1000 °C. At each temperature, axial compressive loads with magnitudes ranging between 15% and 80% of the buckling loads were applied to the top of the column, and the creep buckling failure time was measured to examine its relationship with the compressive load. The stainless steel column was found to fail within a relatively short time compared to that of creep deformation under tensile loading. An increase in the temperature of the column was found to accelerate creep buckling failure. The in-plane and out-of-plane column displacements, which respectively, corresponded to the axial and lateral displacements, were monitored during the entire experiment. The creep buckling behavior of the column was also visualized by a high-speed camera. Based on the Larson–Miller parameters (LMP) determined from the experimental results, an empirical correlation for predicting the creep buckling failure time was developed. Another empirical correlation for predicting the creep buckling failure time based on the lateral deflection rate was also derived.

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DETERMINATION OF MICRO QUANTITIES OF ANDROSTERONE, AETIOCHOLANOLONE AND DEHYDROEPIANDROSTERONE BY GAS-LIQUID CHROMATOGRAPHY

Acta Endocrinologica ◽

10.1530/acta.0.045s071 ◽

1964 ◽

Vol 45 (4_Suppl) ◽

pp. S71-S80 ◽

Cited By ~ 11

Author(s):

John T. France ◽

Neal L. McNiven ◽

Ralph I. Dorfman

Keyword(s):

Stainless Steel ◽

Liquid Chromatography ◽

Trimethylsilyl Ether ◽

Nitrogen Pressure ◽

Gas Liquid Chromatography ◽

Column Temperature ◽

Steel Column ◽

Stainless Steel Column ◽

Reproducible Method

ABSTRACT A convenient, sensitive and reproducible method for the determination of androsterone, aetiocholanolone and dehydroepiandrosterone, as their trimethylsilyl ether derivatives, has been described. The conditions include: 3.3 % XE-60 on Anakrom ABS (90-100 mesh), 6 ft. × 3.8 mm i. d. stainless steel column, 225° column temperature, 327° vaporizer temperature, nitrogen pressure 11.0 p. s. i. (34 ml/min flow). The % variation between duplicate samples for androsterone, aetiocholanolone and dehydroepiandrosterone respectively was 1.5, 4.5 and 1.8 at the 0.3 μg level. Quantitation at the 0.05 μg level appears possible.

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Stainless-Steel Column for Robust, High-Temperature Microchip Gas Chromatography

Analytical Chemistry ◽

10.1021/acs.analchem.8b04174 ◽

2018 ◽

Vol 91 (1) ◽

pp. 792-796 ◽

Cited By ~ 4

Author(s):

Abhijit Ghosh ◽

Austin R. Foster ◽

Jacob C. Johnson ◽

Carlos R. Vilorio ◽

Luke T. Tolley ◽

...

Keyword(s):

Gas Chromatography ◽

Stainless Steel ◽

High Temperature ◽

Steel Column ◽

Stainless Steel Column

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Development and Validation of Novel Ultra Performance Liquid Chromatography Technique for the Simultaneous Determination of Metformin and Repaglinide in Bulk and Formulation

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i33a31778 ◽

2021 ◽

pp. 115-124

Author(s):

H. K. Sundeep Kumar ◽

Suman Acharyya ◽

Pratap Kumar Patra

Keyword(s):

Mobile Phase ◽

Dosage Form ◽

Limit Of Detection ◽

Ultra Performance Liquid Chromatography ◽

Chromatographic Technique ◽

Limit Of Quantification ◽

Study Approach ◽

Study Results ◽

Tablet Dosage ◽

Liquid Chromatographic

A novel ultra-performance liquid chromatographic technique for the estimation of metformin and repaglinide in a API and tablet dosage form. The chromatographic separation was achieved using DIKMA Endoversil (2.1 x 50mm, 1.7µm) column with a mobile phase of phosphate buffer, pH 4.2 and methanol as a mobile phase (38:62) with a flow rate of 0.3 mL/min and the detection wavelength was monitored at 241 nm. The method was validated in accordance with International conference on harmonization guidelines. In this present method metformin was eleued at 0.516 minute and repaglinide was eluted at 1.152 min. Limit of detection was 0.05 μg/ml for metformin and1.152 μg/ml for repaglinide limit of quantification was found 0.5 μg/mL. Calibration curve plots were found linear over the concentration ranges 1-50 μg/mL for both the analytes. The % assay of the marketed dosage form was found 99.45 % for metformin and 97.08 % for repaglinide. The present study approach was found to be effective in the analysis of both analytes in force degradation conditions, because both the analytes has been specifically eluted in presence of other chromatograms. The experiential evidences of all the study results revealed the suitability of the estimation of metformin and repaglinide in API and tablet dosage form.

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A simple reverse phase UPLC validated method for concurrent estimation of Emtricitabine, Darunavir, Tenofovir and Cobicistat in drug substances and drug product

Research Journal of Chemistry and Environment ◽

10.25303/259rjce7988 ◽

2021 ◽

Vol 25 (9) ◽

pp. 79-88

Author(s):

Suresh Gandi ◽

Manikandan Ayyar ◽

Venkat Rao Sirugubattula

Keyword(s):

Flow Rate ◽

Mobile Phase ◽

Drug Product ◽

Chromatographic Technique ◽

Reverse Phase ◽

Drug Substances ◽

Liquid Chromatographic ◽

Concurrent Estimation

A fast, precise, accurate and steady isocratic liquid chromatographic technique was created for the synchronous assurance of the Emtricitabine, Darunavir, Tenofovir and Cobicistat in bulk and in formulation. To optimize a column HSS C18 100 x 2.1 mm, 1.8mm, mobile phase including Buffer 0.01N KH2PO4(5.4pH): Acetonitrile pick in the proportion 60:40v/v was pumped through column at a flow rate of 0.3 ml/min at 260nm. The retention times of Emtricitabine, Darunavir, Tenofovir and Cobicistat were initiated to be 1.066 min, 1.727 min, 2.574 min and 2.977 min. % recovery was 99.75%, 100.05%, 100.42% and 100.59% for Emtricitabine, Darunavir, Tenofovir and Cobicistat respectively. LOD and LOQ values got from relapse formula of Emtricitabine, Darunavir, Tenofovir and Cobicistat and were 0.18, 0.54, 0.71,2.14, 0.01,0.05 0.44 and 1.32 correspondingly. Relapse equation of Emtricitabine is y = 13375x + 2577.4, for Darunavir it is y = 7196.3x + 2981.2, for Tenofovir it is y = 66663x + 1338.9 and y = 22723x + 6978.7 for Cobicistat.

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Extraction Efficiency of a Commercial Espresso Machine Compared to a Stainless-Steel Column Pressurized Hot Water Extraction (PHWE) System for the Determination of 23 Pharmaceuticals, Antibiotics and Hormones in Sewage Sludge

Applied Sciences ◽

10.3390/app9071509 ◽

2019 ◽

Vol 9 (7) ◽

pp. 1509 ◽

Cited By ~ 3

Author(s):

Ola Svahn ◽

Erland Björklund

Keyword(s):

Stainless Steel ◽

Sewage Sludge ◽

Extraction Efficiency ◽

Chemical Properties ◽

Hot Water ◽

Water Extraction ◽

Hot Water Extraction ◽

Pressurized Hot Water Extraction ◽

Steel Column ◽

Stainless Steel Column

Two green chemistry extraction systems, an in-house stainless-steel column Pressurized Hot Water Extraction system (PHWE) and a commercially available Espresso machine were applied for analysing 23 active pharmaceutical ingredients (APIs) in sewage sludge. Final analysis was performed on UPLC-MS/MS using two different chromatographic methods: acid and basic. When analysing all 23 APIs in sewage sludge both extraction methods showed good repeatability. The PHWE method allowed for a more complete extraction of APIs that were more tightly bound to the matrix, as exemplified by much higher concentrations of e.g., ketoconazole, citalopram and ciprofloxacin. In total, 19 out of 23 investigated APIs were quantified in sewage sludge, and with a few exceptions the PHWE method was more exhaustive. Mean absolute recoveries of 7 spiked labelled APIs were lower for the PHWE method than the Espresso method. Under acid chromatographic conditions mean recoveries were 16% and 24%, respectively, but increased to 24% and 37% under basic conditions. The difference between the PHWE method and the Espresso method might be interpreted as the Espresso method giving higher extraction efficiency; however, TIC scans of extracts revealed a much higher matrix co-extraction for the PHWE method. Attempts were made to correlate occurrence of compounds in sewage sludge with chemical properties of the 23 APIs and there are strong indications that both the number of aromatic rings and the presence of a positive charge is important for the sorption processes to sewage sludge.

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