scholarly journals Antioxidant Capacity and Total Phenolic Content in Fruit Tissues from Accessions ofCapsicum chinenseJacq. (Habanero Pepper) at Different Stages of Ripening

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Lizbeth A. Castro-Concha ◽  
Jemina Tuyub-Che ◽  
Angel Moo-Mukul ◽  
Felipe A. Vazquez-Flota ◽  
Maria L. Miranda-Ham

In the past few years, there has been a renewed interest in studying a wide variety of food products that show beneficial effects on human health.Capsicumis an important agricultural crop, not only because its economic importance, but also for the nutritional values of its pods, mainly due to the fact that they are an excellent source of antioxidant compounds, and also of specific constituents such as the pungent capsaicinoids localized in the placental tissue. This current study was designed to evaluate the antioxidant capacity and total phenolic contents from fruits tissues of twoCapsicum chinenseaccessions, namely, Chak k’an-iik (orange) and MR8H (red), at contrasting maturation stages. Results showed that red immature placental tissue, with a Trolox equivalent antioxidant capacity (TEAC) value of 55.59 μmols TE g−1 FW, exhibited the strongest total antioxidant capacity using both the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the CUPRAC methods. Placental tissue also had the highest total phenolic content (27 g GAE 100 g−1 FW). The antioxidant capacity ofCapsicumwas directly related to the total amount of phenolic compounds detected. In particular, placentas had high levels of capsaicinoids, which might be the principal responsible for their strong antioxidant activities.

2014 ◽  
Vol 58 (1) ◽  
pp. 103-111 ◽  
Author(s):  
María C. Ciappini ◽  
Fernando S. Stoppani

Abstract Polyphenolic compounds reportedly produce physiological effects that are beneficial to health. Bee products are particularly rich in polyphenolic compounds. We determined the antioxidant capacity and the phenolic and flavonoid compounds content of 81 samples of honey. We used the Folin-Ciocalteu reagent method to evaluate the total phenolic content. The antioxidant activities were evaluated using in vitro scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH ) and hydroxyl radicals (OH ), Trolox equivalent antioxidant capacity (TEAC ), and ferric-reducing antioxidant capacity (FRAC ). Total phenolic content ranged from 40.3 to 193.0 mg gallic acid equivalents (GAE )/100 g; the flavonoid content varied from 1.4 to 7.5 mg quercetin equivalents (QE)/100 g. Eucalyptus honeys exhibited significantly higher phenolic content and free radical-scavenging activity than clover honey samples (p<0.05 for all). Principal component analysis explained 73% of the differences observed in antiradical activity with respect to floral origin. Total phenolic content may be more useful than the radical-scavenging assay for detecting antioxidant capacity in honey; it also represents the variable that most appropriately discriminated among these honeys.


Genetika ◽  
2020 ◽  
Vol 52 (3) ◽  
pp. 825-834
Author(s):  
Emre Sevindik ◽  
Sinem Aydin ◽  
Mehmet Paksoy ◽  
Bahar Sokmen

The genus Inula belongs to family Asteraceae and it is medicinally important because it possesses cytotoxic, anticancer, hepatoprotective, antibacterial and anti-inflammatory properties. The aim of this research was to explore antioxidant and anti-urease activities of fifteen Inula taxa in Turkey. Ethanol extracts of Inula taxa were obtained by Soxhlet extraction. Antioxidant activity was determined through total antioxidant activity, total phenolic content and copper reducing antioxidant capacity (CUPRAC) analyses. The highest total phenolic content and total antioxidant capacity were found in I. orientalis (158.54?0.002 ?g GAE/mL) and I. macrocephala (189.16?0.002 ?g AAE/mL). CUPRAC activity increases with the increasing concentration of the extracts and the standard. Moreover, all the tested plants exhibited better anti-urease activity than standard thiourea except for I. anatolica and I. thapsoides subsp. thapsoides. Results obtained from this study demonstrate that Inula species might be utilized as a probable natural source in the pharmaceutical and food industries but further and more specific studies are needed to determine active antioxidative and anti-urease compounds in the extracts.


2015 ◽  
Vol 40 (6) ◽  
Author(s):  
Mahfuz Elmastaş ◽  
İsa Telci ◽  
Hüseyin Akşit ◽  
Ramazan Erenler

AbstractObjective: Mint (Mentha spp.) genotypes used as spices are cultivated in Turkey and used for different purposes including herbal tea, spices, the cosmetics industry, and are used in folk medicine. While mint species have been used in traditional practices during humanity’s long history, there is limited research on the comparison of their antioxidant capacity and phenolic contents. This aim of the research is to compare antioxidant capacity and phenolic contents in mint clones to determine superior genotypes for herbal and spice usage and cultivation.Methods: Antioxidant capacity was evaluated by ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC). Total phenolic content in clones were determined by Folin-Ciocalteu method.Results: Maximum total phenolic content [28.27±3.95 μg gallic acid equivalent (GAE)/g dry weight (DW)] and FRAP activity 577.09±46.02 μmol Trolox equiv/g DW were obtained from Clone 13 (M. spicata), while M. piperita clones were higher for TEAC activity (800.02±1.10 μmol Trolox equiv/g DW). The results were first records for M. villoso nervata. FRAP and TEAC activities selected clones were correlated with total phenolic content (r=0.77; 0.73 respectively).Conclusion: According to the results, it can be suggested that Clone 13, Clone 5, (M. spicata) Clone 3 and Clone 8 (M. piperita) are more suitable for use as spice, herbal tea, and antioxidant agents. The clones must be selected for commercial cultivation by the grower and they can be used as spices and herbal teas.


2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Surabhi Shukla ◽  
Radha Kushwaha ◽  
Monika Singh ◽  
Jyotsana Singh ◽  
Vinita Puranik ◽  
...  

Tropical fruits claim to have phenolic compounds that have been reported to possess strong antioxidant activity. The study investigatedantioxidant activity and total phenolic content of five Guava cultivars namely Allahabad Safeda, Allahabad Surkha, Lalit, Chittidar and Apple color harvested at three fruit ripening stages (unripe, semi-ripe and ripe) and extracted by four solvents (ethanol, methanol, acetone and water) with different polarities (50%, 70% and 100%).The findings suggest that guava cultivars with maturity stages and extracting solvent affected its phenolic content and antioxidant activity significantly. Pure solvents were inefficient extraction medium for antioxidants. Enhanced extraction yields were obtained from solvent containing higher water concentrations and 50% ethanol is a recommended solvent for extracting antioxidants compounds from guava fruit. High correlations between phenolic compositions and antioxidant activities of guava extracts were observed. The total phenolics and antioxidant capacity decreases as the ripening proceeds (unripe>semi-ripe>ripe) in all five cultivars.This study facilitates information on the total phenolic content and antioxidant capacities of guava cultivars which is an important commercial fruit. Results obtained showed that solvent polarities and ripening stages played the major role in extraction of ployphenolics from Guava. The significant declines in the levels of total phenolics and antioxidant capacity during fruit development suggest that the unripe fruits can also be taken for processing into nutraceutical foods.


2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Khalid Chatoui ◽  
Hicham Harhar ◽  
Taha El Kamli ◽  
Mohamed Tabyaoui

Lepidium sativum seeds (LSS) from four regions of Morocco have been analyzed for their total chemical composition and antioxidant activities. In the seeds of this plant, the moisture content and yield were, respectively, 9.24–9.88% and 19.13–19.94% of dry weight. Chemical analysis of the seeds revealed amounts of fatty acids, sterols, and tocopherols. The most important fatty acids are linolenic acid (33%) and oleic acid (23%). The main sterol is β-sitosterol (50%); the vegetable oil of Lepidium sativum revealed an amount of tocopherol (∼1500–1900 mg/kg) with dominance of γ-tocopherol. The Folin–Ciocalteu trial evaluated the total phenolic compound, DPPH radical scavenging, ABTS, and chelated iron ions. FRAP measured antioxidant potency. Results indicated that methanol extract from Lepidium sativum was a more potent reducing agent and radical scavenger than ethanol extract. Changes in the total phenolic content and antioxidant capacity of Lepidium sativum in four different regions grown under normal conditions were evaluated. The antioxidant activity of different extracts was found to correlate significantly with their total phenolic content. These results suggest that Lepidium sativum seeds could be used in food supplement preparations or as a food additive, for caloric gain or for protecting against oxidation in nutrient products.


2014 ◽  
Vol 70 (5) ◽  
Author(s):  
Mohsen Marvibaigi ◽  
Neda Amini ◽  
Eko Supriyanto ◽  
Shajarahtunnur Jamil ◽  
Fadzilah Adibah Abdul Majid ◽  
...  

Discovery of new therapeutic agents from nature, especially plants is one of the promising approaches for treatment of various diseases. In traditional medicine Scurrula ferruginea is applied to treat some disorders. To the best of our knowledge, there are no investigations on antioxidant capacity and antimicrobial activities of S.ferruginea in Malaysia. The present study was conducted to determine total phenolic content, Fe2+ chelating activity, antioxidative and antimicrobial potential of flowers, leaves and stems of S.ferruginea extracts. Antioxidant capacity, and total phenolic content of extracts were evaluated using DPPH free radical scavenging and Folin-Ciocalteu assays. Antibacterial properties were evaluated by disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration methods. Results indicated the highest total phenolic content for stem extract (309.069). All S. ferruginea extracts exhibited antioxidant activity in a dose dependent manner. Stem extract showed capacity to scavenge free radicals and it was also found to chelate Fe2+ better than others. All extracts presented moderate inhibition ability against selected bacteria. The most significant values of MIC and MBC were belonged to the stem extract. These findings suggest that acetone extracts of S. ferruginea, particularly stem extract, are potentially sources of antioxidant compounds.


Food Research ◽  
2021 ◽  
Vol 5 (S2) ◽  
pp. 91-97
Author(s):  
S. Ariviani ◽  
K.C. Hapsari ◽  
G. Fauza ◽  
D. Ishartani ◽  
W. Atmaka ◽  
...  

This study was aimed to investigate the total phenolic content and antioxidant activity of elicited pigeon pea (Cajanus cajan) sprouts prepared using various Na-alginate concentrations at different elicitation durations. The germination power and yield of the sprouts were also examined. Elicitation proved capable to improve the antioxidant capacity of legumes sprouts but the yield and germination rates were decreased due to the elicitation stress. In this study, elicitation conducted by immersing pigeon pea seeds in Na -alginate solutions (250, 300, and 350 ppm) for various duration (16, 20, and 24 hrs). The seeds were then germinated for 48 hrs to produce Na-alginate elicited pigeon pea sprouts. The results showed that the total phenolic content (TPC) and antioxidant activity (Trolox equivalent antioxidant capacity, TEAC, and Ferric reducing antioxidant power, FRAP) of elicited pigeon pea sprouts were significantly increased along with the increasing elicitation duration in all Na-alginate levels. The higher Na-alginate levels produce a higher level of TPC, TEAC, and FRAP values. On the other hand, germination power and the yield of the sprouts were significantly decreased along with increasing Na-alginate levels and elicitation duration. Elicitation using 350 ppm Na-alginate with an elicitation duration of 24 hrs produces elicited pigeon pea sprouts with the highest TPC and antioxidant activity, but lowest germination power and yield. These results have an important consequence in developing an elicitation technique to improve the antioxidant capacity of leguminous.


2016 ◽  
Vol 44 (2) ◽  
pp. 28-33 ◽  
Author(s):  
Geovanna Tafurt García ◽  
Luisa Jiménez Vidal ◽  
Ana Calvo Salamanca

<p>In this work, the possible correlation between the antioxidant activities and the Total Phenolic Content (TPC) and chemical composition of Lamiaceae (<em>H. conferta, H. dilatata, H. mutabilis, H. suaveolens</em>), Burseraceae (<em>P. heptaphyllum, T. rhoifoila, T. panamensis</em>),<em> </em>and Lauraceae (<em>Ocotea </em>sp.) were evaluated. The Trolox Equivalent Antioxidant Capacity or the Total Antioxidant Activity (TAA) was determined by using a colorimetric assay with the ABTS radical cation, Effective Concentration (EC<sub>50</sub>) was evaluated with the DPPH radical, and the TPC was established by the Folin-Ciocalteu method, for ethanolic extracts obtained by cold maceration and evaporation to dryness. Both the TAA and the EC<sub>50</sub> were highly correlated with the TPC. The barks of <em>T. rhoifolia</em> and<em> T. panamensis</em> demonstrated the highest antioxidant capacities. The Burseraceae spp. exhibited the highest TPC, and the Lamiaceae (<em>Hyptis</em> spp.) demonstrated the lowest TPC.</p>


2020 ◽  
Vol 21 (6) ◽  
Author(s):  
JANSON CALVINDI ◽  
MUHAMAD SYUKUR ◽  
Waras Nurcholis

Abstract. Calvindi J, Syukur M, Nurcholis W. 2020. Investigation of biochemical characters and antioxidant properties of different winged bean (Psophocarpus tetragonolobus) genotypes grown in Indonesia. Biodiversitas 21: 2420-2424. Winged bean, Psophocarpus tetragonolobus (L) DC, is described as having antioxidant properties. This work evaluated the biochemical and antioxidant characteristics of the P. tetragonolobus genotypes. Twelve-winged bean genotypes were calculated for total phenolic content (TPC) and total flavonoid content (TFC) of biochemical characters and antioxidant properties by using four methods: 2,2-diphenyl picrylhydrazyl (DPPH), cupric reducing antioxidant power (CUPRAC), ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant capacity (TEAC). The plant material was grown in the same location conditions in Indonesia. The total phenolic content varied from 154.6 to 161.5 mg GAE/ 100 g, and flavonoid ranged from 105.2 to 112.4 mg QE/ 100 g fresh weight. The antioxidant capacities were 30.6 - 47.0, 140.4 - 167.6, 66.9 - 170.8, and 28.0 - 52.4 µmol TE/ 100 g fresh weight as calculated by the DPPH, TEAC, FRAP, and CUPRAC assays, respectively. The antioxidant activities were significantly correlated with the polyphenol content of winged bean genotypes fruits. The genotypes TU, L3, H3U, H1P, and TH were recognized higher based on their TPC, TFC, and antioxidant activities, indicating that these genotypes to be promising for further breeding program and commercial purposes.


2020 ◽  
Vol 12 (15) ◽  
pp. 5926
Author(s):  
Po-Hsien Li ◽  
Yung-Jia Chan ◽  
Wen-Chien Lu ◽  
Da-Wei Huang ◽  
Ting-Chieh Chang ◽  
...  

Djulis (Chenopodium formosanum) is a yearly, fast-growing, under-utilized pseudo-cereal with a high proportion of biomass content. We used the hulls, which are usually removed from djulis as crop residue, to evaluate the free-radical scavenging and antioxidant capacity of djulis. We studied the antioxidant capacity of ethanol- and water-extracted hulls and roots by using various in vitro methods. Ascorbic acid was the reference sample. The extract samples were used at 200, 400, 600, 800, and 1000 µg/mL. Total sugar content, total phenolic content, and total flavonoid content were assessed. Antioxidant activity was assessed by using the Trolox equivalent antioxidant capacity, ferric reducing antioxidant power, cupric ion reducing antioxidant capacity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, and N, N-dimethyl-ρ-phenylenediamine. Ethanol- and water-extracted red djulis hulls showed high amounts of total sugar, total phenolic content, total flavonoid content, and antioxidant capacity. Moreover, ethanol- and water-extracted red djulis roots showed moderate antioxidant capacity. However, ethanol- and water-extracted yellow djulis hulls showed limited antioxidant activities. Utilization of the biomass of djulis hulls and roots as natural antioxidant resources may be environmentally friendly and foreseeable.


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