Tracking Transplanted Stem Cells Using Magnetic Resonance Imaging and the Nanoparticle Labeling Method in Urology

A reliablein vivoimaging method to localize transplanted cells and monitor their viability would enable a systematic investigation of cell therapy. Most stem cell transplantation studies have used immunohistological staining, which does not provide information about the migration of transplanted cellsin vivoin the same host. Molecular imaging visualizes targeted cells in a living host, which enables determining the biological processes occurring in transplanted stem cells. Molecular imaging with labeled nanoparticles provides the opportunity to monitor transplanted cells noninvasively without sacrifice and to repeatedly evaluate them. Among several molecular imaging techniques, magnetic resonance imaging (MRI) provides high resolution and sensitivity of transplanted cells. MRI is a powerful noninvasive imaging modality with excellent image resolution for studying cellular dynamics. Several types of nanoparticles including superparamagnetic iron oxide nanoparticles and magnetic nanoparticles have been used to magnetically label stem cells and monitor viability by MRI in the urologic field. This review focuses on the current role and limitations of MRI with labeled nanoparticles for tracking transplanted stem cells in urology.

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Magnetic Resonance Imaging Assessment of Bone Regeneration in Osseous Defects Filled with Different Biomaterials. An experimental in vivo study

Materiale Plastice ◽

10.37358/mp.19.1.5158 ◽

2019 ◽

Vol 56 (1) ◽

pp. 235-238

Author(s):

Tudor Sorin Pop ◽

Alina Dia Trambitas Miron ◽

Anca Maria Pop ◽

Klara Brinzaniuc ◽

Cristian Trambitas

Keyword(s):

Magnetic Resonance Imaging ◽

Stem Cells ◽

Magnetic Resonance ◽

Bone Regeneration ◽

Imaging Modality ◽

Treatment Options ◽

Relaxation Times ◽

Collagen Scaffold ◽

Resonance Imaging

Noninvasive techniques, such as Magnetic Resonance Imaging (MRI) are becoming more used in the study of bone regeneration outcomes, in both animal models and human patients. Many studies have shown the potential of tissue engineering therapies to promote healing of skeletal injuries, but further research is needed to optimize these treatment options. This investigation is a continuation of other previous published studies, in which critical-sized bone defects created in the parietal bones of Wistar rats were filled with either bioglass or a biologic collagen scaffold seeded both with adipose-derived mesenchymal stem cells. The present study aims to present the applications of MRI in the assessment of the bone regeneration process in these previously created defects, at specific time intervals. MRI techniques are based on the differences between the spin-latice T1 and spin-spin T2 relaxation times of examined specimens or areas, compared to normal tissues. We measured the relaxation times specific to calvarial specimens with a Brukner Minispec. The results showed that the most favorable results were observed when a collagen scaffold seeded with stem cells was used. MRI enabled the detection of hard and even soft tissue changes, these findings suggesting that MRI could be an effective imaging modality for assessing changes in bone morphology and pathobiology. Furthermore, it can be used as an alternative to Computed Tomography in examining the role of various biomaterials used in bone healing, especially when ionizing radiation is contraindicated.

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Noninvasive Tracking of Cardiac Embryonic Stem Cells In Vivo Using Magnetic Resonance Imaging Techniques

Stem Cells ◽

10.1634/stemcells.2007-0216 ◽

2007 ◽

Vol 25 (11) ◽

pp. 2936-2944 ◽

Cited By ~ 57

Author(s):

Steven N. Ebert ◽

David G. Taylor ◽

Ha-Long Nguyen ◽

David P. Kodack ◽

Ronald J. Beyers ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Stem Cells ◽

Embryonic Stem Cells ◽

Magnetic Resonance ◽

Imaging Techniques ◽

Embryonic Stem ◽

Resonance Imaging ◽

Magnetic Resonance Imaging Techniques

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In vivo tracking of human adipose-derived stem cells labeled with ferumoxytol in rats with middle cerebral artery occlusion by magnetic resonance imaging

Neural Regeneration Research ◽

10.4103/1673-5374.158355 ◽

2015 ◽

Vol 10 (6) ◽

pp. 909 ◽

Cited By ~ 8

Author(s):

Chang-bin Jiang ◽

Jing Liu ◽

Yan Yin ◽

Xiang Zhou ◽

Xin Guan ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Stem Cells ◽

Magnetic Resonance ◽

Middle Cerebral Artery ◽

Middle Cerebral Artery Occlusion ◽

Artery Occlusion ◽

Adipose Derived Stem Cells ◽

Resonance Imaging ◽

Cerebral Artery Occlusion

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In Vivo Magnetic Resonance Imaging of Intravenously Injected Neural Stem Cells in a Mouse Model of Multiple Sclerosis

The Neuroradiology Journal ◽

10.1177/197140090601900514 ◽

2006 ◽

Vol 19 (5) ◽

pp. 635-636

Author(s):

L.S. Politi ◽

S. Pluchino ◽

M. Bacigaluppi ◽

E. Brambilla ◽

M. Cadioli ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Multiple Sclerosis ◽

Stem Cells ◽

Magnetic Resonance ◽

Mouse Model ◽

Neural Stem Cells ◽

Resonance Imaging

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In Vivo Tracking and 1H/19F Magnetic Resonance Imaging of Biodegradable Polyhydroxyalkanoate/Polycaprolactone Blend Scaffolds Seeded with Labeled Cardiac Stem Cells

ACS Applied Materials & Interfaces ◽

10.1021/acsami.8b06096 ◽

2018 ◽

Vol 10 (30) ◽

pp. 25056-25068 ◽

Cited By ~ 14

Author(s):

Christakis Constantinides ◽

Pooja Basnett ◽

Barbara Lukasiewicz ◽

Ricardo Carnicer ◽

Edyta Swider ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Stem Cells ◽

Magnetic Resonance ◽

Cardiac Stem Cells ◽

Resonance Imaging ◽

19F Magnetic Resonance Imaging

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ECG-gated dynamic magnetic resonance imaging method for examination of the pig heart

Acta Veterinaria Hungarica ◽

10.1556/004.49.2001.3.3 ◽

2001 ◽

Vol 49 (3) ◽

pp. 275-284

Author(s):

Zs. Petrási ◽

R. Romvári ◽

G. Bajzik ◽

B. Fenyves ◽

I. Repa ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Three Dimensional ◽

Dynamic Magnetic Resonance Imaging ◽

Imaging Method ◽

Resonance Imaging ◽

Dynamic Magnetic Resonance ◽

Heart Cycle ◽

The Right

A dynamic magnetic resonance imaging (MRI) method was developed for in vivo examination of the pig heart. Measurements were carried out on 15 meat-type pigs of different liveweight using a 1.5 T equipment. Inhalation anaesthesia was applied, then data acquisition was synchronised by ECG gating. Depending on the heart rate and heart size, in each case 8 to 10 slices and in each slice 8 to 14 phases were acquired prospectively according to one heart cycle. During the post-processing of the images the left and the right ventricular volumes were determined. The values measured at 106 kg liveweight are 2.5 times higher than those obtained at 22 kg, while the ejection fractions are equal. The calculated cardiac output values were 3.5 l (22 kg, 132 beats/min.), and 6.0 l (106 kg, 91 beats/min.), respectively. After measuring the wall thickness, the contraction values were also determined for the septum (70%), and for the anterior (61%), posterior (41%) and lateral (54%) walls of the left ventricle. Three-dimensional animated models of the ventricles were constructed. Based on the investigations performed, the preconditioning, the anaesthetic procedure, the specific details of ECG measurement and the correct MR imaging technique were worked out.

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Visualization of exosomes from mesenchymal stem cells in vivo by magnetic resonance imaging

Magnetic Resonance Imaging ◽

10.1016/j.mri.2020.02.001 ◽

2020 ◽

Vol 68 ◽

pp. 75-82 ◽

Cited By ~ 3

Author(s):

Tianqi Liu ◽

Yurong Zhu ◽

Ruiting Zhao ◽

Xinhua Wei ◽

Xuegang Xin

Keyword(s):

Magnetic Resonance Imaging ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Magnetic Resonance ◽

Resonance Imaging

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The Use of Magnetic Resonance Imaging to Estimate the Mass of the Pectoralis Muscle of Chickens In Vivo

Proceedings of the British Society of Animal Production (1972) ◽

10.1017/s0308229600026015 ◽

1994 ◽

Vol 1994 ◽

pp. 50-50

Author(s):

N.D. Scollan ◽

L.J. Caston ◽

Z. Liu ◽

A.K. Zubair ◽

S. Leeson ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Broiler Chickens ◽

Animal Studies ◽

Whole Body ◽

Imaging Method ◽

Resonance Imaging ◽

Body Images ◽

Tissue Samples

In studies of animal growth it is often necessary to assess whole body composition or organ size prior to and during the course of a particular treatment. Nuclear Magnetic Resonance (NMR) offers the possibility to achieve these measurements on the same animal and in a non-invasive fashion. The use of NMR in attaining body images, referred to as Magnetic Resonance Imaging (MRI), has developed as the imaging method of choice for humans, due to its excellent soft tissue contrast and use of nonionizing radiation. The use of NMR in animal studies has been limited, which is probably related to the availability of suitable facilities and the cost of using them. However, several research groups have applied it to determining fat and water content of tissue samples and intact animals (Mitchell et al., 1991; Scollan et al., 1993). The aim of this study was to evaluate the use of MRI to determine the size (volume) and shape of the Pectoralis muscle (Pectoralis major and minor) in broiler chickens, non-invasively and in vivo.

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Detection of Wooden Foreign Bodies in Muscle Tissue: Experimental Comparison of Computed Tomography, Magnetic Resonance Imaging, and Ultrasonography

Foot & Ankle International ◽

10.1177/107110079401500807 ◽

1994 ◽

Vol 15 (8) ◽

pp. 437-443 ◽

Cited By ~ 58

Author(s):

Mark S. Mizel ◽

Neil D. Steinmetz ◽

Elly Trepman

Keyword(s):

Magnetic Resonance Imaging ◽

Computed Tomography ◽

Magnetic Resonance ◽

Muscle Tissue ◽

Foreign Bodies ◽

Imaging Modality ◽

Experimental Comparison ◽

Imaging Method ◽

Resonance Imaging ◽

Ct And Mri

An experimental study was performed to compare computed tomography (CT), magnetic resonance imaging (MRI), and real-time, high resolution ultrasonography (US) for the detection of wooden foreign bodies in muscle tissue. Wooden splinters were prepared, measured for size, soaked in saline, and placed in porcine muscle distant from and adjacent to bone. The specimens were then examined using roentgenography, CT and MRI in planes parallel and perpendicular to the splinters, and US. The largest wooden foreign bodies (minimum smallest width = 10 mm) were easily detected by CT, MRI, and US. Almost all splinters of various sizes, small and large, soaked in saline for 5 months were easily detected by MRI. Smaller splinters (minimum smallest width = 1–4 mm) soaked for only 3 days and placed distant from bone were most easily detected by US; those soaked for 5 months were most easily detected by either US or MRI. The smaller splinters soaked for only 3 days and placed near bone were not reliably detected by any of the imaging methods; CT and MRI were both more sensitive than US in this situation. MRI scanning was more sensitive perpendicular than parallel to the long axis of the splinters. Therefore, either US or MRI may be the best initial imaging modality for evaluation of a suspected wooden foreign body, depending on availability of imaging method, chronicity of symptoms, and proximity to bone.

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