scholarly journals Magnetic Resonance Imaging Assessment of Bone Regeneration in Osseous Defects Filled with Different Biomaterials. An experimental in vivo study

2019 ◽  
Vol 56 (1) ◽  
pp. 235-238
Author(s):  
Tudor Sorin Pop ◽  
Alina Dia Trambitas Miron ◽  
Anca Maria Pop ◽  
Klara Brinzaniuc ◽  
Cristian Trambitas
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Magnetic Resonance ◽  
Bone Regeneration ◽  
Imaging Modality ◽  
Treatment Options ◽  
Relaxation Times ◽  
Collagen Scaffold ◽  
Resonance Imaging

Noninvasive techniques, such as Magnetic Resonance Imaging (MRI) are becoming more used in the study of bone regeneration outcomes, in both animal models and human patients. Many studies have shown the potential of tissue engineering therapies to promote healing of skeletal injuries, but further research is needed to optimize these treatment options. This investigation is a continuation of other previous published studies, in which critical-sized bone defects created in the parietal bones of Wistar rats were filled with either bioglass or a biologic collagen scaffold seeded both with adipose-derived mesenchymal stem cells. The present study aims to present the applications of MRI in the assessment of the bone regeneration process in these previously created defects, at specific time intervals. MRI techniques are based on the differences between the spin-latice T1 and spin-spin T2 relaxation times of examined specimens or areas, compared to normal tissues. We measured the relaxation times specific to calvarial specimens with a Brukner Minispec. The results showed that the most favorable results were observed when a collagen scaffold seeded with stem cells was used. MRI enabled the detection of hard and even soft tissue changes, these findings suggesting that MRI could be an effective imaging modality for assessing changes in bone morphology and pathobiology. Furthermore, it can be used as an alternative to Computed Tomography in examining the role of various biomaterials used in bone healing, especially when ionizing radiation is contraindicated.

scholarly journals Tracking Transplanted Stem Cells Using Magnetic Resonance Imaging and the Nanoparticle Labeling Method in Urology

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Jae Heon Kim ◽  
Hong J. Lee ◽  
Yun Seob Song
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Molecular Imaging ◽  
Magnetic Resonance ◽  
Imaging Modality ◽  
Imaging Techniques ◽  
Image Resolution ◽  
Imaging Method ◽  
Resonance Imaging

A reliablein vivoimaging method to localize transplanted cells and monitor their viability would enable a systematic investigation of cell therapy. Most stem cell transplantation studies have used immunohistological staining, which does not provide information about the migration of transplanted cellsin vivoin the same host. Molecular imaging visualizes targeted cells in a living host, which enables determining the biological processes occurring in transplanted stem cells. Molecular imaging with labeled nanoparticles provides the opportunity to monitor transplanted cells noninvasively without sacrifice and to repeatedly evaluate them. Among several molecular imaging techniques, magnetic resonance imaging (MRI) provides high resolution and sensitivity of transplanted cells. MRI is a powerful noninvasive imaging modality with excellent image resolution for studying cellular dynamics. Several types of nanoparticles including superparamagnetic iron oxide nanoparticles and magnetic nanoparticles have been used to magnetically label stem cells and monitor viability by MRI in the urologic field. This review focuses on the current role and limitations of MRI with labeled nanoparticles for tracking transplanted stem cells in urology.

scholarly journals Dynamic contrast-enhanced magnetic resonance imaging for monitoring neovascularization during bone regeneration—a randomized in vivo study in rabbits

2021 ◽  
Author(s):  
L. A. R. Righesso ◽  
M. Terekhov ◽  
H. Götz ◽  
M. Ackermann ◽  
T. Emrich ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Bone Regeneration ◽  
Blood Perfusion ◽  
Autogenous Bone ◽  
Resonance Imaging ◽  
Dce Mri ◽  
Dynamic Contrast Enhanced ◽  
Contrast Enhanced

Abstract Objectives Micro-computed tomography (μ-CT) and histology, the current gold standard methods for assessing the formation of new bone and blood vessels, are invasive and/or destructive. With that in mind, a more conservative tool, dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), was tested for its accuracy and reproducibility in monitoring neovascularization during bone regeneration. Additionally, the suitability of blood perfusion as a surrogate of the efficacy of osteoplastic materials was evaluated. Materials and methods Sixteen rabbits were used and equally divided into four groups, according to the time of euthanasia (2, 3, 4, and 6 weeks after surgery). The animals were submitted to two 8-mm craniotomies that were filled with blood or autogenous bone. Neovascularization was assessed in vivo through DCE-MRI, and bone regeneration, ex vivo, through μ-CT and histology. Results The defects could be consistently identified, and their blood perfusion measured through DCE-MRI, there being statistically significant differences within the blood clot group between 3 and 6 weeks (p = 0.029), and between the former and autogenous bone at six weeks (p = 0.017). Nonetheless, no significant correlations between DCE-MRI findings on neovascularization and μ-CT (r =−0.101, 95% CI [−0.445; 0.268]) or histology (r = 0.305, 95% CI [−0.133; 0.644]) findings on bone regeneration were observed. Conclusions These results support the hypothesis that DCE-MRI can be used to monitor neovascularization but contradict the premise that it could predict bone regeneration as well.

In Vivo Magnetic Resonance Imaging of Intravenously Injected Neural Stem Cells in a Mouse Model of Multiple Sclerosis

2006 ◽  
Vol 19 (5) ◽  
pp. 635-636
Author(s):  
L.S. Politi ◽  
S. Pluchino ◽  
M. Bacigaluppi ◽  
E. Brambilla ◽  
M. Cadioli ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Multiple Sclerosis ◽  
Stem Cells ◽  
Magnetic Resonance ◽  
Mouse Model ◽  
Neural Stem Cells ◽  
Resonance Imaging

scholarly journals In Vivo Tracking and 1H/19F Magnetic Resonance Imaging of Biodegradable Polyhydroxyalkanoate/Polycaprolactone Blend Scaffolds Seeded with Labeled Cardiac Stem Cells

2018 ◽  
Vol 10 (30) ◽  
pp. 25056-25068 ◽  
Author(s):  
Christakis Constantinides ◽  
Pooja Basnett ◽  
Barbara Lukasiewicz ◽  
Ricardo Carnicer ◽  
Edyta Swider ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Magnetic Resonance ◽  
Cardiac Stem Cells ◽  
Resonance Imaging ◽  
19F Magnetic Resonance Imaging

Visualization of exosomes from mesenchymal stem cells in vivo by magnetic resonance imaging

2020 ◽  
Vol 68 ◽  
pp. 75-82 ◽  
Author(s):  
Tianqi Liu ◽  
Yurong Zhu ◽  
Ruiting Zhao ◽  
Xinhua Wei ◽  
Xuegang Xin
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Magnetic Resonance ◽  
Resonance Imaging

Non-invasive tracking of human haemopoietic CD34+ stem cells in vivo in immunodeficient mice by using magnetic resonance imaging

2010 ◽  
Vol 20 (9) ◽  
pp. 2184-2193 ◽  
Author(s):  
Markus Niemeyer ◽  
Robert A. J. Oostendorp ◽  
Markus Kremer ◽  
Sandra Hippauf ◽  
Volker R. Jacobs ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Immunodeficient Mice ◽  
Non Invasive

scholarly journals Multimodal evaluation of in vivo magnetic resonance imaging of myocardial restoration by mouse embryonic stem cells

2008 ◽  
Vol 136 (4) ◽  
pp. 1028-1037.e1 ◽  
Author(s):  
Stephen L. Hendry ◽  
Koen E.A. van der Bogt ◽  
Ahmad Y. Sheikh ◽  
Takayasu Arai ◽  
Scott J. Dylla ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Magnetic Resonance ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Resonance Imaging
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