scholarly journals Impact of Atmospheric Microparticles on the Development of Oxidative Stress in Healthy City/Industrial Seaport Residents

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Kirill Golokhvast ◽  
Tatyana Vitkina ◽  
Tatyana Gvozdenko ◽  
Victor Kolosov ◽  
Vera Yankova ◽  
...  
Keyword(s):  
Energy State ◽  
Oxidative Modification ◽  
System Response ◽  
Energy Potential ◽  
Healthy City ◽  
Oxidative Modification Of Proteins ◽  
Industrial Areas ◽  
Total Antioxidant ◽  
Different Levels ◽  
Snow Samples

Atmospheric microsized particles producing reactive oxygen species can pose a serious health risk for city residents. We studied the responses of organisms to microparticles in 255 healthy volunteers living in areas with different levels of microparticle air pollution. We analyzed the distribution of microparticles in snow samples by size and content. ELISA and flow cytometry methods were employed to determine the parameters of the thiol-disulfide metabolism, peroxidation and antioxidant, genotoxicity, and energy state of the leukocytes. We found that, in the park areas, microparticles with a size of 800 μm or more were predominant (96%), while in the industrial areas, they tended to be less than 50 μm (93%), including size 200–300 nm (7%). In the industrial areas, we determined the oxidative modification of proteins (21% compared to the park areas,p≤0.05) and DNA (12%,p≤0.05), as well as changes in leukocytes’ energy potential (53%,p≤0.05). An increase in total antioxidant activity (82%,p≤0.01) and thiol-disulfide system response (thioredoxin increasing by 33%,p≤0.01; glutathione, 30%,p≤0.01with stable reductases levels) maintains a balance of peroxidation-antioxidant processes, protecting cellular and subcellular structures from significant oxidative damage.

Correction of Free Radical Blood Imbalance and Behaviour in Prenatally Stressed Rats with α-Tocopherol

2021 ◽  
pp. 25-34
Author(s):  
Olʼga N. Kuleshova ◽  
◽  
David L. Teplyy ◽  
Dmitriy D. Teplyy
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Oxidative Modification ◽  
Male Rats ◽  
Total Antioxidant Activity ◽  
Oxidative Modification Of Proteins ◽  
Total Antioxidant ◽  
Prenatally Stressed ◽  
Ceruloplasmin Activity

The research involved female mongrel white rats (n = 16) and their offspring (sexually mature males, n = 75). Pregnant females were divided into 4 groups: control (intact animals), stress (from the 16th to the 19th day of pregnancy females were subjected to 3-hour immobilization in plastic cases), stress+α-tocopherol (against the background of immobilization, females received α-tocopherol at a dose of 1 mg per 100 g of weight starting from the 2nd day of pregnancy), and α-tocopherol (females received α-tocopherol in the same dose and regimen as the group above). The control and stress groups received vegetable oil instead of α-tocopherol. In 3-month-old female offspring, we studied behaviour and determined the levels of products of free radical modification of proteins and lipids, total antioxidant activity as well as superoxide dismutase and ceruloplasmin activity in the blood plasma. Prenatal stress increased the exploratory and motor activity in offspring and modified the level of free radical homeostasis in the serum of male rats: there was an increase in the level of products of oxidative modification of proteins, thiobarbituric acid reactive substances concentration, end products of lipid peroxidation (Schiff bases), total antioxidant activity and ceruloplasmin activity, as well as a decrease in superoxide dismutase activity. Alpha-tocopherol taken by females during pregnancy brought all the oxidation parameters modified by immobilization to the values of the control group; the levels of primary products of oxidative modification of proteins and lipid peroxidation decreased two-fold, compared with the stress group. The activity of superoxide dismutase and ceruloplasmin returned to the control values, but the total antioxidant activity remained at the level of the stress group. Thus, there is reason to assume that α-tocopherol has a significant corrective effect on the intensity of free radical processes in prenatally stressed white male rats.

The evaluation of lipid peroxidation and oxidative modification of proteins in blood serum under obesity development and the consumption of aqueous kidney beans Phaseolus vulgaris pods extract

2020 ◽  
Vol 33 (1) ◽  
pp. 38-44
Author(s):  
Alona Yurchenko ◽  
Daryna Krenytska ◽  
Olexii Savchuk ◽  
Tetiana Halenova ◽  
Natalia Raksha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Modification ◽  
Resistance Development ◽  
Oxidative Modification Of Proteins ◽  
Kidney Beans ◽  
Calorie Diet ◽  
Diet Control ◽  
High Calorie Diet ◽  
High Calorie

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.

THE INTENSITY OF OXIDATIVE MODIFICATION OF PROTEINS AND PEROXIDATION OF LIPIDS IN THE BLOOD SERUM, BRAIN, LIVER AND PANCREAS TISSUES OF RATS WITH INSULIN RESISTANCE AND CONGENITAL IODINE DEFICIENCY

2019 ◽  
pp. 115-120
Author(s):  
V. B. Stetsevyat ◽  
N. M. Voronych-Semchenko
Keyword(s):  
Insulin Resistance ◽  
Iodine Deficiency ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
High Fructose ◽  
Liver And Pancreas ◽  
Dependent Processes

The results of the study about the nature of oxygen-dependent processes in rats that were on a high-fructose diet for 8 weeks under conditions of adequate iodine supply and congenital iodine deprivation is presented in the article. The significant activation of peroxide oxidation of proteins (by increasing the aldo- and keto-derivates of a neutral nature) and lipids (increased of diene conjugates content and products, that are responsible to thiobarbituric acid) of animals with insulin resistance was found. An aggravating factor in the course of these processes is a congenital iodine deficiency. The changes of oxidative modification of proteins in animals with congenital iodine deficiency had multidirectional character, and processes of lipid peroxidation mainly increased. The most pronounced changes of the studied processes were observed in the liver and pancreas of animals with insulin resistance against the background of congenital iodine deficiency.

ПОКАЗАТЕЛИ И МЕТОДЫ ОЦЕНКИ ЭНЕРГЕТИЧЕСКОГО ПОТЕНЦИАЛА ПОЧВЫ В АГРОЛАНДШАФТАХ

2020 ◽  
pp. 10
Author(s):  
V.Yu. Pavlov
Keyword(s):  
Energy State ◽  
Energy Potential

В статье рассмотрены вопросы оценки энергетического потенциала почв. Приведены методики его расчета. Определена совокупность показателей для оценки энергетического состояния почв и приведены методики для их определения.The article deals with the assessment of the energy potential of soils. Methods of its calculation are given. A set of indicators for assessing the energy state of soils is determined and methods for determining them are given.

Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain

2006 ◽  
Vol 27 (1) ◽  
pp. 42-53 ◽  
Author(s):  
Minna A. Korolainen ◽  
Gundars Goldsteins ◽  
Tuula A. Nyman ◽  
Irina Alafuzoff ◽  
Jari Koistinaho ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Frontal Cortex ◽  
Oxidative Modification ◽  
Oxidative Modification Of Proteins

scholarly journals Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Sahoko Ichihara ◽  
Yuka Suzuki ◽  
Jie Chang ◽  
Kentaro Kuzuya ◽  
Chisa Inoue ◽  
...  
Keyword(s):  
Atp Synthesis ◽  
Oxidative Modification ◽  
Oxidative Modification Of Proteins

Oxidative modification of proteins and phospholipids in rotenone-induced neurotoxicity in PC12 cells

2016 ◽  
Vol 96 ◽  
pp. S68
Author(s):  
Elisabetta Chiaradia ◽  
Fiorella D'Onofrio ◽  
Angela Gambelunghe ◽  
Lorena Urbanelli ◽  
Carla Emiliani ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Oxidative Modification ◽  
Oxidative Modification Of Proteins

scholarly journals Improving 'Piyarom' date palm fruit quality with fruit thinning and bunch covering treatments

2021 ◽  
Vol 35 (1) ◽  
pp. 11-19
Author(s):  
Babak Madani ◽  
Abdolmajid Mirzaalian Dastjerdy ◽  
Ali Shahriyari
Keyword(s):  
Fruit Quality ◽  
Date Palm ◽  
Ascorbic Acid Content ◽  
Total Phenolic ◽  
Positive Role ◽  
Palm Fruit ◽  
Fruit Thinning ◽  
Date Fruit ◽  
Total Antioxidant ◽  
Different Levels

‘Piyarom’ dates are one of the most important commercial date cultivars grown in Iran but fruit quality and postharvest losses can be a problem. Thinning and the use of bunch covers were examined to improve the date fruit quality. Three different levels of fruit thinning were applied (without thinning, removal of one third of total strands from terminal tips during pollination and removal of one third of terminal tips of central strands in early kimri). In addition, the effects of bunch covers were assessed with the use of polyethylene bunch covers that were applied in the early stages of Khalal. The results showed that bunch covering reduced the lightness of the fruit but increased bunch weight, fruit length, total antioxidant activity and total phenolic compounds compared to the control. Thinning during pollination increased the weight of the fruit compared to the control. The thinning during Kimri stage increased the percentage of Tamar and ascorbic acid content. These results show that the use of covers play a positive role in increasing quality and bunch weight and thinning at both pollination and Kimri ripening stage is recommended to produce high quality ‘Piyarom’ dates.

scholarly journals PROSPECTS FOR THE USE OF THE PARAMETERS OF OXIDATIVE MODIFICATION OF SERUM PROTEINS FOR ASSESSMENT OF THE AGONAL PERIOD DURATION

2019 ◽  
Vol 5 (3) ◽  
pp. 28-32
Author(s):  
I. S. Edelev ◽  
L. M. Obukhova ◽  
N. A. Andriyanova ◽  
N. S. Edelev
Keyword(s):  
Serum Proteins ◽  
Oxidative Modification ◽  
Evidence Based ◽  
Natural Death ◽  
Oxidative Modification Of Proteins ◽  
Carbonyl Derivatives ◽  
Forensic Examination ◽  
Autopsy Data ◽  
Period Duration ◽  
Relevant Factors

In the article we present results of the studies in cases of death with a long agonal period to determine the length of the agony.Objectives. The task was to examine the features of oxidative modification of serum proteins in cases of deaths with a long agonal period.Material and methods. In all experiments we analyzed the liquid blood sampled from 54 corpses of persons of both sexes who died from the violent and natural death in age of 13 to 89 years. The assessment of the oxidative modification of proteins (OMP) was performed by the level of carbonyl derivatives detected in the reaction with 2,4-dinitrophenylhydrazine. The levels of absolute and relative (in terms of protein) total OMP in cases of long agonal period were compared with the counterpart levels in cases of the short agony. The duration of the agonу was determined using information about the circumstances of the case and investigative data with the permission of the authority ordered the forensic examination. The autopsy data also were taken into account.Results. We revealed the dependence of the OMP level on the duration of the agonal period. The total OMP (in terms of protein) in cases of long agony have decreased by 45.46 %. The data obtained have showed that the parameters of the OMP, taking into account all the relevant factors, can be used to develop criteria for establishing the duration of the agonal period as well as to increase the evidence-based significance of medico-legal conclusions.

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