scholarly journals Bovine Serum Albumin and Chitosan Coated Silver Nanoparticles and Its Antimicrobial Activity against Oral and Nonoral Bacteria

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
León Francisco Espinosa-Cristóbal ◽  
Gabriel Alejandro Martínez-Castañón ◽  
Juan Pablo Loyola-Rodríguez ◽  
Nereyda Niño-Martínez ◽  
Facundo Ruiz ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Drug Resistance ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Antimicrobial Agents ◽  
Reduction Method ◽  
Gram Positive Bacteria ◽  
Transmission Electron

Antimicrobial agents have been developed for drug-resistance infections, which have been rapidly increasing; however, the control of involved microorganisms is still a challenge. In this work, SNP with bovine serum albumin (BSA) and chitosan (CS) coatings were prepared with an aqueous reduction method, characterized using dispersion light scattering, transmission electron microscopy, and thermal analysis. Antibacterial activity was tested on seven oral and nonoral bacteria by microdilution test and scanning electron microscopy. Six different sizes and shapes of coated SNP were prepared and used. Characterization revealed narrow size and good distribution of particles, spherical and pseudospherical shapes, and the presence of coatings on the SNP surfaces. All samples showed antimicrobial activity, although smaller sizes and CS samples had the best inhibition effects. The highest microbial resistance was shown by Gram-positive bacteria. Although coated SNP action depends on particular bacterium, BSA and CS coated SNP could be used for drug-resistance infections.

Bovine Serum Albumin Conjugation in Superparamagnetic/Poly(methyl methacrylate) Nanoparticles as an Alternative for Magnetic Enzyme-Linked Immunosorbent Assays

2021 ◽  
Vol 21 (11) ◽  
pp. 5493-5498
Author(s):  
Paulo Emilio Feuser ◽  
Camila Guindani ◽  
Jonathann Correa Possato ◽  
Jaqueline Pereira Guessi ◽  
Arthur Poester Cordeiro ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Bovine Serum ◽  
Protein Quantification ◽  
Elisa Assay ◽  
Poly Methyl Methacrylate ◽  
Transmission Electron ◽  
Superparamagnetic Properties ◽  
Significant Attention

Nanomaterials, such as magnetic nanoparticles have attracted significant attention of medical area due to their capacity to improve the performance of immunoassays. Therefore the aim of this work was to study the bovine serum albumin (BSA) conjugation in superparamagnetic (MNPs)/poly(methyl methacrylate) (PMMA) nanoparticles with further characterization and application in enzyme-linked immunosorbent (ELISA) assay. The successful conjugation of BSA in MNPs- PMMA nanoparticles was confirmed by several techniques, including light scattering, zeta potential, transmission electron microscopy (TEM) and Lowry protein quantification assay. The superparamagnetic properties were confirmed by vibrating sample magnetometer. BSA conjugated MNPs-PMMA nanoparticles presented higher interactions with antibody than free BSA. The BSA + MNPs-PMMA nanoparticles (magnetic ELISA assay) reduced the time and increased the sensibility of traditional ELISA assay, reinforcing the idea that the use these nanomaterials are an excellent alternative for the immunoassays field.

Surface Morphology Investigation of Poly(ether ether ketone) Immobilized with Heparin and Bovine Serum Albumin

2013 ◽  
Vol 683 ◽  
pp. 409-412
Author(s):  
Hui Sun ◽  
Rui Chao Chen ◽  
Biao Yang ◽  
Guo Zhi Xu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Surface Morphology ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Ether Ether Ketone ◽  
Poly Ether Ether Ketone ◽  
Ether Ketone ◽  
Scanning Electron

The surface topography of poly(ether ether ketone) (PEEK) film immobilized with heparin and Bovine Serum Albumin (BSA) was characterized via scanning electron microscopy (SEM). Compared with the unmodified film, the surface of modified film changed and become rough, which indirectly proved the successful introduction of monomer and biomolecule on PEEK

A nitro-capped tetraaniline derivative with AIE features for BSA detection and the selective imaging of Gram-positive bacteria

2019 ◽  
Vol 43 (30) ◽  
pp. 11816-11820 ◽  
Author(s):  
Beibei Liu ◽  
Kun Wang ◽  
Hao Lu ◽  
Mingming Huang ◽  
Jiping Yang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Turn On

A nitro-capped tetraaniline derivative (NO2-B3-Ani4-NO2) with AIE feature was synthesized and characterized, which presented an ultrasensitive turn-on response towards bovine serum albumin and selective imaging of Gram-positive bacteria based on AIE mechanism.

Synthesis, Characterization and Biological Studies of a Organoselenium trans-Palladium(II) Complexes

2020 ◽  
Vol 16 ◽  
Author(s):  
Ivana Raković ◽  
Jovana Bogojeski ◽  
Katarina Mladenović ◽  
Angelina Petrović ◽  
Vera Divac ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell Line ◽  
Spectral Studies ◽  
Cytotoxic Effects ◽  
Hct 116

Background: Over the years, transition metal complexes have exhibited significant antimicrobial and antitumor activity. It all started with cisplatin discovery, but due to the large number of side effects it shows, there is a growing need to find a new metal-based compound with higher selectivity and activity on more tumors. Objectives: Two novel trans-palladium(II) complexes with organoselenium compounds as ligands, [Pd(L1)2Cl2] (L1 = 5- (phenylselanylmethyl)-dihydrofuran-2(3H)-one) and [Pd(L2)2Cl2] (L2 = 2-methyl-5-(phenylselanylmethyl)- tetrahydrofuran) were synthesized, in the text referred to as Pd-Se1 and Pd-Se2. Also, a structurally similar trans-palladium(II) complex, [Pd(L3)2Cl2] (L3= 2,2-dimethyl-3-(phenylselanylmethyl)-tetrahydro-2H-pyran ) was synthesized according to an already published work and is referred to as Pd-Se3. The interaction of synthesized complexes with DNA and bovine serum albumin were done. Also, antimicrobial activity and in vitro testing, cell viability, and cytotoxic effects of synthesized ligands and complexes on human epithelial colorectal cancer cell line HCT-116 were studied. Molecular docking simulations were performed to understand better the binding modes of the complexes reported in this paper with DNA and BSA, as well as to comprehend their antimicrobial activity. Methods: The interactions of the synthesized complexes with DNA and bovine serum albumin were done using UV-Vis and emission spectral studies as well as docking studies. Antimicrobial activity was tested by determining the minimum inhibitory concentrations (MIC) and minimum microbicidal concentration (MMC) using the resazurin microdilution plate method. Cytotoxic activity on cancer cells was studied by MTT test. Results: The Pd(II) complexes showed a significant binding affinity for calf thymus DNA and bovine serum albumin by UV-Vis and emission spectral studies. The intensity of antimicrobial activity varied with the complexes Pd-Se1 and Pd-Se3, showing significantly higher activity than the corresponding ligand. The most significant activity was shown on Pseudomo-nas aeruginosa. Under standardized laboratory conditions for in vitro testing, cell viability and cytotoxic effects of synthesized ligands and complexes were studied on human epithelial colorectal cancer cell line HCT-116, where Pd-Se2 showed some significant cytotoxic effects. Conclusion: The newly synthesized complexes have the potential to be further investigated as metallodrugs.

scholarly journals Design and development of paclitaxel-loaded bovine serum albumin nanoparticles for brain targeting

2011 ◽  
Vol 61 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Amit Bansal ◽  
Deepak Kapoor ◽  
Rishi Kapil ◽  
Neha Chhabra ◽  
Sanju Dhawan
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Correlation Spectroscopy ◽  
Brain Targeting ◽  
Release Mechanism ◽  
Design And Development ◽  
Albumin Nanoparticles

Design and development of paclitaxel-loaded bovine serum albumin nanoparticles for brain targeting Bovine serum albumin (BSA) nanoparticles loaded with paclitaxel (PTX) were prepared using a desolvation technique. A 32 full factorial design (FFD) was employed to formulate nanoparticles. Nanoparticles were characterized for particle size by photon correlation spectroscopy and surface morphology by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Encapsulation efficiency, zeta potential and particle yield were also determined. Response surface linear modelling (RSLM) was used to predict the optimal formulation. Various models were applied to determine the release mechanism from PTX nanoparticles. The effect of drug-polymer ratio on the release profile of formulations was observed and was applied to determine the suitability of the predicted optimal formulation. A preliminary study to determine the feasibility of targeting the prepared nanoparticles to brain was also carried out using mice as in vivo models.

Antimicrobial Activity and Interaction with Bovine Serum Albumin of Nickel(II) Complex

2012 ◽  
Vol 554-556 ◽  
pp. 1831-1834
Author(s):  
Yu Fen Liu ◽  
Hai Tao Xia ◽  
De Fu Rong
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ground State ◽  
Bovine Serum ◽  
Binding Constants ◽  
Quenching Mechanism ◽  
Binding Reaction ◽  
Physiological Conditions

The binding reaction of nickel(II) complex [Ni(C16H20N2)2•(H2O)2]Cl2•C3H7NO with bovine serum albumin(BSA) was studied by fluorescence spectroscopy under the simulative physiological conditions. The experimental results show that the fluorescence quenching of BSA by nickel(II) complex is a result of the formation of ground state complex and the quenching mechanism was static quenching. The binding constants were 4.24×103L•mol−1at 293K with one binding site. The antimicrobial activity study found that the nickel(II) complex was active against Escherichia coli, Staphylococcus aureus and Bacillus subtilis.

Inhibition of Sodium Hypochlorite Antimicrobial Activity in the Presence of Bovine Serum Albumin

2010 ◽  
Vol 36 (2) ◽  
pp. 268-271 ◽  
Author(s):  
Fernanda Geraldes Pappen ◽  
Wei Qian ◽  
Jolanta Aleksejūnienė ◽  
Renato de Toledo Leonardo ◽  
Mario Roberto Leonardo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Sodium Hypochlorite ◽  
Bovine Serum

Cationic double chained metallosurfactants: synthesis, aggregation, cytotoxicity, antimicrobial activity and their impact on the structure of bovine serum albumin

Soft Matter ◽  
2018 ◽  
Vol 14 (25) ◽  
pp. 5306-5318 ◽  
Author(s):  
Gurpreet Kaur ◽  
Preeti Garg ◽  
Baljinder Kaur ◽  
Ganga Ram Chaudhary ◽  
Sandeep Kumar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protein Binding ◽  
Bovine Serum ◽  
Metal Ion

The insertion of the metal ion into the framework of the surfactant structure enhances its aggregation and protein binding/folding/unfolding abilities.

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