scholarly journals Functionalised Au Coated Iron Oxide Nanocomposites Based Reusable Immunosensor for AFB1 Detection

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Ruchika Chauhan ◽  
T. Basu

A reusable sandwiched electrochemical piezoelectric immunosensor has been developed for aflatoxin B1 (AFB1) detection using gold coated iron oxide core-shell (Au-Fe3O4) nanostructure. The monoclonal anti-aflatoxin antibody (aAFB1) was immobilized on self-assembled monolayer of 4-aminothiophenol on gold coated quartz crystal to fabricate immunoelectrode (BSA/aAFB1/4-ATP/Au). In addition, secondary rabbit-immunoglobulin antibodies (r-IgGs) functionalized with Au-Fe3O4NPs via cysteamine (r-IgG-Cys-Au-Fe3O4) were allowed to interact with AFB1. Both competitive and noncompetitive strategies were employed and a competition between coated AFB1 and free AFB1 was carried out. The competitive mode shows higher linear range (0.05 to 5 ng mL−1) than the noncompetitive one (0.5 to 5 ng mL−1), high sensitivity 335.7 µA ng−1 mL cm−2, and LOD 0.07 ng mL−1. The fabricated immunosensor has been tested using cereal samples spiked with different concentrations of AFB1. The developed competitive immunoelectrode displays good reproducibility, and storage stability and regenerated with negligible loss in activity through removal of the r-IgG-Cys-Au-Fe3O4conjugate using a strong external magnet.

2009 ◽  
Vol 38 (Supplement-1) ◽  
pp. 87-97 ◽  
Author(s):  
H. Daood

During the last decades comprehensive data in the field of chemistry, biology and technology of spice red pepper (paprika) have rapidly accumulated. In food science and technology the research work focused on, among others, developing accurate, simple, rapid, reliable, and of high sensitivity analytical methods to determine paprika pigments and antioxidants. The research concentrated mainly on the development and optimisation of liquid chromatographic methods to determine the carotenoids, tocopherols (vitamin E) and ascorbic acid (vitamin C). In the area of plant biology/physiology characterisation of ripening of traditional and new cultivars and hybrids of spice red pepper was the main objective of research work carried out by research groups in different countries. In the field of paprika processing and technology special interest has been given to the effect of different drying technologies on the quality components of paprika and their stability during drying, milling and storage. The aim of many research programs was to produce paprika with high colouring capacity, antioxidant content and storage stability. The objective of this article is to review the research works done on spice red pepper in the Central Food Research Institute, Budapest.


Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1397
Author(s):  
Nappaphan Kunanusont ◽  
Boonchai Sangpetngam ◽  
Anongnat Somwangthanaroj

Plastic waste has been incorporated with asphalt to improve the physical properties of asphalt and alleviate the increasing trend of plastic waste being introduced into the environment. However, plastic waste comes in different types such as thermoplastic or thermoset, which results in varied properties of polymer modified asphalt (PMA). In this work, four thermoplastic vulcanizates (TPVs) were prepared using different peroxide concentrations to produce four formulations of gel content (with varying extent of crosslinked part) in order to imitate the variation of plastic waste. All four TPVs were then mixed with asphalt at 5 wt% thus producing four formulations of PMA, which went through physical, rheological, and storage stability assessments. PMA with higher gel content possessed lower penetration and higher softening temperature, indicating physically harder appearance of PMA. Superpave parameters remained unchanged among different gel content PMA at temperatures of 64, 70, and 76 °C. PMA with any level of gel content had lower Brookfield viscosity than PMA without gel content at a temperature of 135 °C. Higher gel content resulted in shorter storage stability measured with greater different softening temperatures between top and bottom layers of PMA after 5 days of 163 °C storage. This study shows that asphalt with thermoset plastic waste is harder and easier to pave, thus making the non-recycling thermoset plastic waste more useful and friendly to the environment.


Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 47
Author(s):  
Julius Rami ◽  
Caroline Dumler ◽  
Nadine Weber ◽  
Michael Rychlik ◽  
Gabriele Netzel ◽  
...  

Folate (Vitamin B9) is critical for a range of biological functions in adults and children, including DNA, protein and neurotransmitter synthesis. It is also essential for the healthy development of the fetus in early pregnancy and for the prevention of neural tube defects, such as spina bifida. Strawberries are considered a tasty and healthy fruit consumed all over the world and may potentially be an important dietary source of natural folates. However, the relative importance of strawberry as a dietary source will depend on the total folate concentration, vitamer profile, storage stability and bioavailability to humans. Red Rhapsody, an important commercial strawberry cultivar in Australia, was screened for its folate content and storage stability by stable isotope dilution assay (SIDA). Total folate content ranged from 90–118 μg/100 g fresh weight (fw), which was well above the value in the Australian Food Composition Database (39 μg/100 g fw). 5-Methyltetrahydrofolate, the biologically active form in humans, was the principal vitamer present. Furthermore, folate remained relatively stable during refrigerated (4 °C) storage (loss of only 28% after 14 days of storage). This information is relevant for consumers since the inherent perishability of strawberry fruit makes refrigerated storage common practice in Australian households.


Author(s):  
Cindy T. Sepúlveda ◽  
Ailén Alemán ◽  
José E. Zapata ◽  
M. Pilar Montero ◽  
M. Carmen Gómez-Guillén

2021 ◽  
Author(s):  
Lidia Rosado Piquer ◽  
Jan Dreiser ◽  
E. Carolina Sañudo

The heterometallic 3d-4f SMM [Co4Dy(OH)2(SALOH)5(chp)4(MeCN)(H2O)2] (1) has been deposited onto iron oxide nanoparticles (NP) with an oleate self-assembled monolayer (SAM) as surfactant. The hybrid molecular-inorganic system 1-NP has been thoroughly...


LWT ◽  
2021 ◽  
pp. 111463
Author(s):  
Tingting Hong ◽  
Qiyan Zhao ◽  
Dan Xu ◽  
Yirong Yuan ◽  
Yue Ma ◽  
...  

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