Folate in Red Rhapsody Strawberry—Content and Storage Stability.

Folate (Vitamin B9) is critical for a range of biological functions in adults and children, including DNA, protein and neurotransmitter synthesis. It is also essential for the healthy development of the fetus in early pregnancy and for the prevention of neural tube defects, such as spina bifida. Strawberries are considered a tasty and healthy fruit consumed all over the world and may potentially be an important dietary source of natural folates. However, the relative importance of strawberry as a dietary source will depend on the total folate concentration, vitamer profile, storage stability and bioavailability to humans. Red Rhapsody, an important commercial strawberry cultivar in Australia, was screened for its folate content and storage stability by stable isotope dilution assay (SIDA). Total folate content ranged from 90–118 μg/100 g fresh weight (fw), which was well above the value in the Australian Food Composition Database (39 μg/100 g fw). 5-Methyltetrahydrofolate, the biologically active form in humans, was the principal vitamer present. Furthermore, folate remained relatively stable during refrigerated (4 °C) storage (loss of only 28% after 14 days of storage). This information is relevant for consumers since the inherent perishability of strawberry fruit makes refrigerated storage common practice in Australian households.

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The Inside and out of Folate in Strawberries and Avocados

Proceedings ◽

10.3390/proceedings2019036086 ◽

2020 ◽

Vol 36 (1) ◽

pp. 86

Author(s):

Netzel ◽

Dumler ◽

Weber ◽

Striegel ◽

Rychlik ◽

...

Keyword(s):

Active Form ◽

Spherical Shape ◽

Food Composition ◽

Biologically Active ◽

Breeding Lines ◽

Dietary Folate ◽

Stable Isotope Dilution ◽

Isotope Dilution Assay ◽

Growing Environment ◽

Selection Of

Folate, an important B-group vitamin, is considered a critical vitamin in many countries, with folate deficiency being associated with neural tube defects in newborns. Strawberries and avocados are considered a healthy, tasty snack by many consumers, and may potentially be an important dietary source of natural folates, depending on variety and growing environment. A selection of Australian-grown strawberry varieties and breeding lines, as well as commercial avocado cultivars, were screened for their folate content and vitamer profile by stable isotope dilution assay. Total folate content ranged from 69–170 μg/100 g fresh weight (fw) for strawberries and 76–196 μg/100 g fw for avocados, which was well above the values in the Australian Food Composition Database (39 μg/100 g fw for strawberries and 90 μg/100 g fw for avocados, respectively). Furthermore, folate concentration in the outer strawberry tissue was found to be 1.7-fold higher than the inner tissue of the fruit, whereas the inner avocado tissue had 1.4-fold higher folate than the outer green edible tissue. 5-Methyltetrahydrofolate, the biologically active form in humans, was the principal vitamer present. With these high folate concentrations, a punnet (250 g) of Australian-grown strawberries or 200 g of Australian-grown avocados would deliver the FSANZ recommended dietary intake (RDI) for folate (400 μg dietary folate equivalents/day/adult). Furthermore, the differences between outer and inner tissue could indicate that flatter, longer strawberries may have greater potential to accumulate folate than fruit with a more spherical shape, whereas more folate could be accumulated in a rounder-shaped avocado.

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Asphalt Incorporation with Ethylene Vinyl Acetate (EVA) Copolymer and Natural Rubber (NR) Thermoplastic Vulcanizates (TPVs): Effects of TPV Gel Content on Physical and Rheological Properties

Polymers ◽

10.3390/polym13091397 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1397

Author(s):

Nappaphan Kunanusont ◽

Boonchai Sangpetngam ◽

Anongnat Somwangthanaroj

Keyword(s):

Storage Stability ◽

Ethylene Vinyl Acetate ◽

Softening Temperature ◽

Plastic Waste ◽

Gel Content ◽

Modified Asphalt ◽

C Storage ◽

Thermoplastic Vulcanizates ◽

Polymer Modified Asphalt ◽

And Storage

Plastic waste has been incorporated with asphalt to improve the physical properties of asphalt and alleviate the increasing trend of plastic waste being introduced into the environment. However, plastic waste comes in different types such as thermoplastic or thermoset, which results in varied properties of polymer modified asphalt (PMA). In this work, four thermoplastic vulcanizates (TPVs) were prepared using different peroxide concentrations to produce four formulations of gel content (with varying extent of crosslinked part) in order to imitate the variation of plastic waste. All four TPVs were then mixed with asphalt at 5 wt% thus producing four formulations of PMA, which went through physical, rheological, and storage stability assessments. PMA with higher gel content possessed lower penetration and higher softening temperature, indicating physically harder appearance of PMA. Superpave parameters remained unchanged among different gel content PMA at temperatures of 64, 70, and 76 °C. PMA with any level of gel content had lower Brookfield viscosity than PMA without gel content at a temperature of 135 °C. Higher gel content resulted in shorter storage stability measured with greater different softening temperatures between top and bottom layers of PMA after 5 days of 163 °C storage. This study shows that asphalt with thermoset plastic waste is harder and easier to pave, thus making the non-recycling thermoset plastic waste more useful and friendly to the environment.

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Folate content in sea buckthorn berries and related products (Hippophaë rhamnoides L. ssp. rhamnoides): LC-MS/MS determination of folate vitamer stability influenced by processing and storage assessed by stable isotope dilution assay

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-008-1905-3 ◽

2008 ◽

Vol 391 (1) ◽

pp. 211-219 ◽

Cited By ~ 18

Author(s):

Derek Gutzeit ◽

Sabine Mönch ◽

Gerold Jerz ◽

Peter Winterhalter ◽

Michael Rychlik

Keyword(s):

Stable Isotope ◽

Isotope Dilution ◽

Sea Buckthorn ◽

Stable Isotope Dilution Assay ◽

Stable Isotope Dilution ◽

Isotope Dilution Assay ◽

Dilution Assay ◽

Processing And Storage ◽

And Storage

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Processing and Characterization of Recombinant von Willebrand Factor Expressed in Different Cell Types Using a Vaccinia Virus Vector

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648398 ◽

1992 ◽

Vol 67 (01) ◽

pp. 154-160 ◽

Cited By ~ 14

Author(s):

P Meulien ◽

M Nishino ◽

C Mazurier ◽

K Dott ◽

G Piétu ◽

...

Keyword(s):

Vaccinia Virus ◽

Von Willebrand Factor ◽

Human Fibroblasts ◽

Active Form ◽

Cell Types ◽

Biologically Active ◽

L Cells ◽

Von Willebrand ◽

Different Cell Types ◽

Willebrand Factor

SummaryThe cloning of the cDNA encoding von Willebrand factor (vWF) has revealed that it is synthesized as a large precursor (pre-pro-vWF) molecule and it is now clear that the prosequence or vWAgll is responsible for the intracellular multimerization of vWF. We have cloned the complete vWF cDNA and expressed it using a recombinant vaccinia virus as vector. We have characterized the structure and function of the recombinant vWF (rvWF) secreted from five different cell types: baby hamster kidney (BHK), Chinese hamster ovary (CHO), human fibroblasts (143B), mouse fibroblasts (L) and primary embryonic chicken cells. Forty-eight hours after infection, the quantity of vWF antigen found in the cell supernatant varied from 3 to 12 U/dl depending on the cell type. By SDS-agarose gel electrophoresis, the percentage of high molecular weight forms of vWF varied from 39 to 49% relative to normal plasma for BHK, CHO, 143B and chicken cells but was less than 10% for L cells. In all cell types, the two anodic subbands of each multimer were missing. The two cathodic subbands were easily detected only in BHK and L cells. By SDS-PAGE of reduced samples, pro-vWF was present in similar quantity to the fully processed vWF subunit in L cells, present in moderate amounts in BHK and CHO and in very low amounts in 143B and chicken cells. rvWF from all cells bound to collagen and to platelets in the presence of ristocetin, the latter showing a high correlation between binding efficiency and degree of multimerization. rvWF from all cells was also shown to bind to purified FVIII and in this case binding appeared to be independent of the degree of multimerization. We conclude that whereas vWF is naturally synthesized only by endothelial cells and megakaryocytes, it can be expressed in a biologically active form from various other cell types.

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Studying of Physical and Chemical Properties of tomato Seed Oil and Storage Stability

Basrah Journal of Agricultural Sciences ◽

10.33762/bagrs.2016.120266 ◽

2016 ◽

Vol 29 (2) ◽

pp. 266-274

Author(s):

Bushra B. Jerd ◽

Ali A. Sahi

Keyword(s):

Seed Oil ◽

Storage Stability ◽

Chemical Properties ◽

Physical And Chemical Properties ◽

Tomato Seed ◽

Physical And Chemical ◽

And Storage ◽

And Chemical Properties ◽

Tomato Seed Oil

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Innovative approaches to the correction of chronotrope status of pregnant and lactating women

HEALTH OF WOMAN ◽

10.15574/hw.2016.114.37 ◽

2016 ◽

pp. 37-40

Author(s):

S.I. Zhuk ◽

◽

K.K. Bondarenko ◽

Keyword(s):

Folic Acid ◽

Neural Tube ◽

Active Form ◽

Biologically Active ◽

Lactating Women ◽

Pathological Conditions ◽

Prevention And Treatment ◽

The Impact ◽

High Bioavailability ◽

Key Words Mthfr

Most recent studies show the impact of violations in the metabolism of folate and metin period in the pathogenesis of neural tube defects (NTD) of the fetus. Metafolin has a number of advantages, which primarily includes direct intake of substances in biologically active form and the optimum effect, even in the case when the patient homozygote and/or heterozygote genotype 677С T polymorphism in MTHFR. With the aim of prevention and treatment of various pathological conditions related to folate deficiency during pregnancy, it is advisable to apply vitamin-mineral complexes, containing metafolin - active form of folate with high bioavailability. Key words: MTHFR, metafolin, folic acid, pregnancy.

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