Comparison of Oxidative Stresses Mediated by Different Crystalline Forms and Surface Modification of Titanium Dioxide Nanoparticles

Titanium dioxide nanoparticles (TiO2NPs) are manufactured worldwide for use in a wide range of applications. There are two common crystalline forms of TiO2anatase and rutile with different physical and chemical characteristics. We previously demonstrated that an increased DNA damage response is mediated by anatase crystalline form compared to rutile. In the present study, we conjugated TiO2NPs with polyethylene glycol (PEG) in order to reduce the genotoxicity and we evaluated some oxidative stress parameters to obtain information on the cellular mechanisms of DNA damage that operate in response to TiO2NPs different crystalline forms exposure in hepatocarcinoma cell lines (HepG2). Our results indicated a significant increase in oxidative stress mediated by the anatase form of TiO2NPs compared to rutile form. On the other hand, PEG modified TiO2NPs showed a significant decrease in oxidative stress as compared to TiO2NPs. These data suggested that the genotoxic potential of TiO2NPs varies with crystalline form and surface modification.

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Titanium dioxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in human amnion epithelial (WISH) cells

Toxicology in Vitro ◽

10.1016/j.tiv.2011.12.011 ◽

2012 ◽

Vol 26 (2) ◽

pp. 351-361 ◽

Cited By ~ 155

Author(s):

Quaiser Saquib ◽

Abdulaziz A. Al-Khedhairy ◽

Maqsood A. Siddiqui ◽

Faisal M. Abou-Tarboush ◽

Ameer Azam ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Human Amnion

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Deleterious effects of sunscreen titanium dioxide nanoparticles on DNA: efforts to limit DNA damage by particle surface modification

10.1117/12.430765 ◽

2001 ◽

Cited By ~ 31

Author(s):

Nick Serpone ◽

Angela Salinaro ◽

A. Emeline

Keyword(s):

Dna Damage ◽

Titanium Dioxide ◽

Surface Modification ◽

Titanium Dioxide Nanoparticles ◽

Particle Surface

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Genotoxic damage in workers exposed to pigment-grade titanium dioxide (TiO2) during paint production

European Journal of Public Health ◽

10.1093/eurpub/ckaa166.1370 ◽

2020 ◽

Vol 30 (Supplement_5) ◽

Author(s):

S a Bonetta ◽

M Macrì ◽

M Acito ◽

S i Bonetta ◽

G Castrignanò ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Titanium Dioxide ◽

Ultrafine Particles ◽

Management Practices ◽

The European Union ◽

Genotoxic Effects ◽

Early Effect ◽

Genotoxic Damage ◽

Wide Range

Abstract TiO2 is broadly used in a wide range of applications such as photocatalysis, pigments and additives. Despite the promising commercial opportunities, different studies have shown that TiO2 can induce oxidative stress, inflammatory and genotoxic effects. The European Union has classified certain titanium dioxide powders and powder mixtures containing TiO2 as a suspected carcinogen (Category 2) via inhalation. Considering that the information about the potential adverse health effects of TiO2 in occupational environment are still scarce and controversial, the purpose of the study was to investigate the genotoxic effects and oxidative stress in workers exposed to TiO2 during paint production. Biomarkers of early effect (DNA damage and micronuclei) were evaluated in the buccal cells and salivary leucocytes of 30 workers (15 production workers and 15 controls). To collect information about personal details, occupational history, medication, smoking, diet, physical activity, a questionnaire was administered to all workers. Personal and area monitoring have been carried out to determine airborne inhalable and respirable fraction of TiO2; a NanoTracer was used to monitor the presence of ultrafine particles. In spite of the low mass concentration and a mild percentage of nanoparticles, filter deposited TiO2 was in sub-micron size, thus accounting for its respirability. Preliminary results on salivary leucocytes show a slight higher DNA damage in the exposed workers as compared to the controls. Whereas biomarker assessment is still in progress, these preliminary findings show that workers are exposed to low but measurable levels of TiO2 able to induce a mild genotoxic damage. A combined approach using both personal exposure assessment and biomonitoring can improve the risk assessment in occupational settings in which TiO2 is handled. Moreover, this also suggest to take precautionary measure during specific activities and operational phases thus decreasing the risks for worker. Key messages The results of the present study may promote effective risk management practices in occupational environments that uses TiO2. The results obtained suggest the introduction of activities and operational phases with lower risks for the worker.

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Cytotoxicity, DNA damage, and apoptosis induced by titanium dioxide nanoparticles in human non-small cell lung cancer A549 cells

Environmental Science and Pollution Research ◽

10.1007/s11356-014-3717-7 ◽

2014 ◽

Vol 22 (7) ◽

pp. 5519-5530 ◽

Cited By ~ 62

Author(s):

Yurong Wang ◽

Haiyan Cui ◽

Jiaping Zhou ◽

Fengjuan Li ◽

Jinju Wang ◽

...

Keyword(s):

Lung Cancer ◽

Dna Damage ◽

Titanium Dioxide ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Titanium Dioxide Nanoparticles ◽

A549 Cells ◽

Small Cell ◽

Small Cell Lung

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Quercetin and Idebenone Ameliorate Oxidative Stress, Inflammation, DNA damage, and Apoptosis Induced by Titanium Dioxide Nanoparticles in Rat Liver

Dose-Response ◽

10.1177/1559325818812188 ◽

2018 ◽

Vol 16 (4) ◽

pp. 155932581881218 ◽

Cited By ~ 10

Author(s):

Laila M. Fadda ◽

Hanan Hagar ◽

Azza M. Mohamed ◽

Hanaa M. Ali

Keyword(s):

Titanium Dioxide ◽

Histopathological Examination ◽

Titanium Dioxide Nanoparticles ◽

Reactive Protein ◽

Wide Range ◽

Factor Α ◽

Immunoglobin G ◽

Endothelial Growth Factor

Titanium dioxide nanoparticles (TiO2-NPs) are extensively used in a wide range of applications; however, many reports have investigated their nanotoxicological effect at the molecular level either in vitro or in vivo systems. The defensive roles of quercetin (Qur) or idebenone (Id) against the hepatotoxicity induced by TiO2-NPs were evaluated in the current study. The results showed that the coadministration of Qur or Id to rats intoxicated with TiO2-NPs markedly ameliorated the elevation in hepatic malondialdehyde (MDA), serum alanine amino-transferase (ALT), glucose, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), immunoglobin G (IgG), and C-reactive protein (CRP) levels compared to their levels in TiO2-NPs-treated rats. The aforementioned antioxidants also effectively modulated the changes in the levels of serum vascular endothelial growth factor (VEGF), nitric oxide (NO), hepatic DNA breakage, caspase-3, and inhibition of drug metabolizing enzymes (cytochrome P450s; CYP4502E12E1) in rat livers induced by TiO2-NPs toxicity. The histopathological examination of the liver section showed that TiO2-NPs caused severe degeneration of most hepatocytes with an increase in collagen in the portal region, while treatment with the antioxidants in question improved liver architecture. These outcomes supported the use of Qur and Id as protective agents against the hepatotoxicity induced by TiO2-NPs and other hepatotoxic drugs.

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Titanium Dioxide Nanoparticles Induce DNA Damage in Peripheral Blood Lymphocytes from Polyposis coli, Colon Cancer Patients and Healthy Individuals: An Ex Vivo/In Vitro Study

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2017.14691 ◽

2017 ◽

Vol 17 (12) ◽

pp. 9274-9285 ◽

Cited By ~ 5

Author(s):

Malgorzata Kurzawa-Zegota ◽

Vyom Sharma ◽

Mojgan Najafzadeh ◽

P. Dominic Reynolds ◽

Justin P Davies ◽

...

Keyword(s):

Colon Cancer ◽

Dna Damage ◽

Titanium Dioxide ◽

Ex Vivo ◽

Titanium Dioxide Nanoparticles ◽

In Vitro Study ◽

Peripheral Blood Lymphocytes ◽

Healthy Individuals ◽

Polyposis Coli

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SURFACE MODIFICATION OF TITANIUM DIOXIDE NANOPARTICLES WITH ACRYLIC ACID/ISOBUTYLENE COPOLYMER UNDER ULTRASONIC TREATMENT

Periódico Tchê Química ◽

10.52571/ptq.v16.n32.2019.356_periodico32_pgs_338_344.pdf ◽

2019 ◽

Vol 16 (32) ◽

pp. 338-344

Author(s):

Nikolay A. BULYCHEV ◽

Lev N. RABINSKIY

Keyword(s):

Titanium Dioxide ◽

Surface Modification ◽

Composite Materials ◽

Acrylic Acid ◽

Ultrasonic Treatment ◽

Titanium Dioxide Nanoparticles ◽

Polymer Coatings ◽

Pigment Surface ◽

Electrokinetic Sonic Amplitude ◽

Modification Of Titanium

The influence of the ultrasonic treatment of block copolymer solution on its solid-liquid interface behavior was investigated in detail. The surface modification of titanium dioxide nanoparticles in aqueous dispersions of specially tailor-made periodic acrylic acid/isobutylene copolymer by ultrasonic treatment was studied in order to get new approaches for the creation of hybrid composite materials or polymer coatings. The pigment surface modification by the above copolymer was comparatively investigated regarding conventional adsorption as contrasted to an ultrasonic treatment assisted procedure. The course and efficiency of the polymer adsorption onto the pigment surface were quantified by electrokinetic sonic amplitude measurements. The higher efficiency of the pigment surface coating by the copolymer as achieved by ultrasonic treatment in comparison to conventional adsorption is a consequence of ultrasonically induced pigment surface activation. Two perspective avenues of the utilization of the discovered effects for creation of organic-inorganic composite materials are anticipated: the nanoparticles could first be treated by ultrasound in the presence of polymers and so create a surface modifying coating and the second option is an entrainment of the nanoparticles into the monomer matrix which can be polymerized afterward yielding a polymer with immobilized nanoparticles.

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