scholarly journals Phytofabrication of Bioactive Molecules Encapsulated Metallic Silver Nanoparticles fromCucumis sativusL. and Its Enhanced Wound Healing Potential in Rat Model

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Perumal Venkatachalam ◽  
Palanivel Sangeetha ◽  
Natesan Geetha ◽  
Shivendra Vikram Sahi
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Rat Model ◽  
Wound Repair ◽  
Bioactive Molecules ◽  
Metallic Silver ◽  
Standard Drug ◽  
Resonance Band ◽  
Ointment Base ◽  
Wound Healing Activity

The present study describes a rapid method for synthesis of metallic silver nanoparticles using callus (CAgNPs) and leaf extracts (LEAgNPs) ofCucumis sativusand evaluation of its wound healing activity in rat model. The prepared silver nanoparticles showed a peak at 350 nm corresponding to the surface plasmon resonance band. The FTIR spectroscopy measurements showed the presence of the possible biomolecules. X-ray diffraction analysis confirmed the crystalline structure of the synthesized silver nanoparticles. TEM images showed the size of the synthesized CAgNPs with diameter ranged from 21 nm to 23 nm with polygonal shape whereas, in the case of LEAgNPs, spherical shape was noticed with an average size between 11 nm and 19 nm. The EDX results indicated the chemical composition at specific locations on synthesized nanoparticles. Furthermore the topical application of ointment prepared using synthesized AgNPs was found to show enhanced wound healing activity in Wistar albino rat model. By the 21st day, the ointment base containing 5% (w/w) of silver nanoparticles showed 100% potential wound healing activity than the standard drug as well as control bases. Results strongly showed that the ointment base containing LEAgNPs was found to be very effective in wound repair mechanism in the experimental rats.

Comparison of the ficus for the wound healing activity in various species

2020 ◽  
Vol 10 (4) ◽  
pp. 67-70
Author(s):  
Mothilal K ◽  
Akila CR ◽  
Mahender K ◽  
Chaitanya Kumar K ◽  
Ravi D
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Subcutaneous Tissue ◽  
Significant Activity ◽  
Herbal Extracts ◽  
Standard Drug ◽  
Excision Wound ◽  
Injuries And Wounds ◽  
Skin Conditions ◽  
Wound Healing Activity

Injuries and wounds are any sorts of damage to the skin or subcutaneous tissue. Usually, any wounds of such sorts are self-healed. Sometimes, there may be a delay in healing, and that delay is caused due to the functional delays in various processes of wound healing. All the Ficus plants show similar activities like the antioxidant, anti-inflammatory and wound healing properties 7including skin conditions like ulcers and rheumatism. The anthelmintic property and immunomodulatory are also seen. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. All the extracts showed a better healing ability, but the extract of FBO-100 showed the highest activity followed by FMO followed by FHO and finally the FRO. Overall, the activity of the extract ointment was comparable and was significant compared to the standard drug ointment. The wound closure of the extract ointment treated groups were better and were completed in 12 days, and the activity was more than 96%. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. The plants of microcarpa, benghalensis, religiosa and hispida are compared for the activity, and the order showed for the activity was FBO>FMO>FHO>FRO.

Sustained Wound Healing Activity of Curcumin Loaded Oleic Acid Based Polymeric Bandage in a Rat Model

2012 ◽  
Vol 9 (10) ◽  
pp. 2801-2811 ◽  
Author(s):  
Chandana Mohanty ◽  
Manasi Das ◽  
Sanjeeb K. Sahoo
Keyword(s):  
Wound Healing ◽  
Oleic Acid ◽  
Rat Model ◽  
Wound Healing Activity

Eugenol-acacia gum-based bifunctional nanofibers as a potent antifungal transdermal substitute

Nanomedicine ◽  
2021 ◽  
Author(s):  
Payal Gupta ◽  
Purusottam Mishra ◽  
Lalita Mehra ◽  
Kartikey Rastogi ◽  
Ramasare Prasad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inclusion Complex ◽  
Polyvinyl Alcohol ◽  
Rat Model ◽  
Candida Species ◽  
Antibiofilm Activity ◽  
Acacia Gum ◽  
Rat Experiments ◽  
Wound Healing Activity

Aim: Fungal biofilms interfere with the wound healing processes. Henceforth, the study aims to fabricate a biomaterial-based nano-scaffold with the dual functionalities of wound healing and antibiofilm activity. Methods: Nanofibers comprising acacia gum, polyvinyl alcohol and inclusion complex of eugenol in β-cyclodextrin (EG-NF) were synthesized using electrospinning. Antibiofilm studies were performed on Candida species, and the wound-healing activity was evaluated through an in vivo excision wound rat model. Results: The EG-NF potentially eradicated the mature biofilm of Candida species and their clinical isolates. Further, EG-NF also enhanced the re-epithelization and speed of wound healing in in vivo rat experiments. Conclusion: The study established the bifunctional applications of eugenol nanofibers as a transdermal substitute with antifungal potency.

Evaluation of Wound Healing Activity of Vanda roxburghii R.Br(Orchidacea): A Preclinical Study in a Rat Model

2005 ◽  
Vol 4 (4) ◽  
pp. 200-204 ◽  
Author(s):  
B. S. Nayak ◽  
R. Suresh ◽  
A. V. C. Rao ◽  
G. K. Pillai ◽  
E. M. Davis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Preclinical Study ◽  
Wound Healing Activity

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

scholarly journals IN VITRO WOUND HEALING AND ANTIMICROBIAL PROPERTY OF COTTON FABRICS COATED OPTIMIZED SILVER NANOPARTICLES SYNTHESIZED USING PELTOPHORUM PTEROCARPUM LEAF EXTRACTS

2019 ◽  
pp. 216-222
Author(s):  
ANNAMALAI P ◽  
BALASHANMUGAM P ◽  
KALAICHELVAN PT
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Incubation Temperature ◽  
Cotton Fabrics ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Potential Measurement ◽  
Healing Property ◽  
Wound Healing Activity ◽  
Wound Healing Property

Objective: The present study was aimed to study at investigating the antibacterial potential of silver nanoparticles (AgNPs) coated cotton fabrics against different pathogens and also for their wound healing property using fibroblasts cells. Materials and Methods: The leaf extracts of Peltophorum pterocarpum were used for the synthesis of AgNPs and were characterizing using ultraviolet-visible spectrophotometer, transmission electron microscopy, energy dispersive X-ray (EDX) spectroscopy, dynamic light scattering analysis, and zeta potential measurement. The AgNPs were coated on cotton fabrics and tested for their antibacterial efficacy using agar well diffusion method. The wound healing property of synthesized AgNPs was tested using fibroblast 3T3 cells. Results: The plant extracts of P. pterocarpum were utilized for AgNPs. The optimum condition for synthesizing AgNPs was found to be 1 mg/ml plant concentration, 7 pH, 1 mM silver nitrate concatenation, and incubation temperature of 37°C. The shape of synthesized AgNPs was found to be spherical with an average size between 20 and 50 nm, and elemental silver peaks were confirmed by EDX spectrum. The cotton fabrics coated with AgNPs show good zone of inhibition against all the tested pathogens and the treated fabrics were also characterized using scanning electron microscope which reveals the presence of AgNPs on the fabrics. The scratch assay reveals that the AgNPs have good wound healing activity when tested against fibroblast 3T3. Conclusion: The present results conclude that the synthesized AgNPs have good stability with potent antimicrobial activity when coated with cotton fabrics. The AgNPs also found to have good activity significant wound healing activity when tested using fibroblast cells.

scholarly journals Correction: Synthesis of DNA-guided silver nanoparticles on a graphene oxide surface: enhancing the antibacterial effect and the wound healing activity

RSC Advances ◽  
2019 ◽  
Vol 9 (67) ◽  
pp. 39434-39434
Author(s):  
Chunyi Tong ◽  
Wei Zou ◽  
Weimin Ning ◽  
Jialong Fan ◽  
Li Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Antibacterial Effect ◽  
Oxide Surface ◽  
Wound Healing Activity

Correction for ‘Synthesis of DNA-guided silver nanoparticles on a graphene oxide surface: enhancing the antibacterial effect and the wound healing activity’ by Chunyi Tong et al., RSC Adv., 2018, 8, 28238–28248.

scholarly journals Wound repair and regeneration potential of the fruits of Terminalia bellarica

2015 ◽  
Vol 4 (5) ◽  
pp. 253-258
Author(s):  
S. Kirubanandan ◽  
◽  
S. Renganathan ◽  
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Granulation Tissue ◽  
Wound Repair ◽  
Treated Group ◽  
Bioactive Molecules ◽  
Synergistic Activity ◽  
Regeneration Potential ◽  
Soxhlet Apparatus

The infection at the wound site is a severe kind of problem and it delays regeneration of epidermis and dermis in the wound and slows wound closure. Due to the secretion of microbial enzymes by wound pathogens, a variety of extracellular matrix proteins were degraded. Synthetic antimicrobial therapy used in the wound management and eradication of pathogens. However, it has many shortcomings such as anti-microbial resistance, cyto-toxicity against host tissue and absence of synergistic activity. In order to overcome these limitations, Pyto-pharmaceuticals extracted from herbal plants were applied to manage the wound infection and treatment. The objective of this work is to evaluate the wound repair and regeneration potential of the fruits of Terminalia bellarica which has a variety of pharmacological activities such as astringent, antiseptic and laxative. The dry fruits of Terminalia bellarica were grounded into powder form using the grinder. Extraction was performed by using Soxhlet apparatus with 95% (v/v) ethanol. The dried extract was dissolved in Dimethyl Sulfoxide (DMSO) and used to assay the antibacterial activity against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853. An ointment was prepared from the ethanol extract (10% w/w) and assessed for its in vivo wound healing potential on infected rat model by rate of healing, bacterial count, biochemical analysis, and expression of matrix metalloproteinase. In addition to that, the collagen content in the granulation tissue was estimated to comment on wound regeneration potential of the fruits of Terminalia bellarica. The treated group has shown significantly improved wound regeneration and well formed epidermis and dermis in the granulation tissue. Furthermore, Assessment of granulation tissue on every fourth day showed significant reduction in bacterial pathogens CFU with significant elevated level of collagen, hexosamine, uronic acid, in the treated group (P<0.05). The reduced level expression of matrix metalloproteinase expression observed in the treated group by gelatin zymography and the synthesis of substantial amount of collagen in the granulation tissue confirms our in vivo assessment. The results showed the antibacterial and wound healing activities of Terminalia bellarica fruits ointment, necessary for the management of infected open dermal wounds. The isolation of bioactive molecules from Terminalia bellarica fruits and its interaction various cells using cell culture studies would be future work.

scholarly journals Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardiumoccidentale Leaves in Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 2061-2068
Author(s):  
Meena Kumari K ◽  
Amberkar Mohanbabu Vittalrao ◽  
Praveen Kumar SE ◽  
Sushma Prabhath ◽  
Charitha Charitha
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Saline Control ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Healing Activity

BACKGROUND -This study undertaken to screen a potential wound healing activity of an ethanolic extract of Anacardiumoccidentale(Ao)leaves in Wistar rats. MATERIALS AND METHODS-Excision wound of 500mm2created on the back of Wistar rats. A total of thirty adult Wistar rats were prescreened and divided into five groups of six rats each.Group I and II rats were treated by normal saline (control) and povidone-iodine (standard drug), respectively. Group III and IV with 0.5% and 2%of Anacardiumoccidentale ointment (test drugs), respectively, and Group V rats treated with anti-wound healer drug dexamethasone, i.m. + 2% Anacardiumoccidentale. All drugs were applied topically except Inj. dexamethasone. In the model of excision wound, the wound healing effect was determined by parameters such as the percentage of wound contraction, duration of epithelialization, and histopathological examination. RESULT-All drugs, povidone-iodine, 0.5%, and 2% of the Ao ointment treated group of rats showed a statistically significant increase (P<0.05) in the % of wound contraction on 5th and 6thdays Vs.Control rats. In contrast,povidone-iodine, 2% Ao ointment and (dexamethasone + 2% Ao)treated rats decreased the period of epithelialization which was statistically significant (P<0.05) compared to the control.However, the standard povidone-iodine treated rats showed a maximum increase in the percentage of wound contraction and minimum period of epithelialization, followed by a higher dose of 2% Ao extract. The plant extract 0.5% and 2% of Ao showeda significant increase inthe rate of wound contraction as compared to the standard drug povidone-iodine also. The combination of (2%Aoextract+dexamethasone) drug-treated rats decreased the rate of wound contraction on 5th, 9th days; whereas, povidone-iodine increased on all days, i.e., 5th, 9th, 13th and 17th days,which was statistically significant (p<0.05) Vs.Normal saline. The povidone and 2% of Ao extract hastened the rate of wound healing activity, which was evident by maximum re-epithelialization and presence of the collagen fibers. CONCLUSION-In our study, the topical application of Anacardiumoccidentale leaf extract has shown promising wound healing activity; however, it failed to reverse the anti-wound healing property of steroids.

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