Assessment of the Wound Healing Property of the Ethanolic Seed Extract of Eleusine coracana Linn using the Chorioallantoic Membrane Model

Access to readily available material for wound healing is very important. The wound healing property of Eleusine coracana a food source in Nigeria was therefore investigated. The ethanolic crude extract of the seed flour was prepared using maceration and qualitative phytochemical screening was carried out on the crude extract using standard methods. The antimicrobial activity of the E. coracana extract was determined using the disc diffusion method against E. coli, S. aureus, P. aeruginosa, and C. tetani. The wound healing property assay was carried out using the Chorioallantoic Membrane Model (CAM) at 50mg, 100mg, 150mg, and 200mg/ml concentration with the basic Fibroblast Growth Factor (bFGF) as the positive control. The crude extract was further fractionated into Hexane, Methanolic, and Aqueous fractions and then subjected to amino acid content analysis using the Applied Biosystems Phenylthiohydantoin (PTH) Amino Acid Analyzer. The phytochemical screening reveals the presence of alkaloids, steroids, terpenes, flavonoids, carbohydrates, glycoside, and protein. E. coli and S. aureus were susceptible to the E. coracana extract at a Minimum Inhibitory Concentration (MIC) of 50µg/ml while the P. aeruginosa was only susceptible at 200µg/ml and C. tetani was not susceptible at all the concentrations used. An increase in percentage angiogenesis was observed at all doses of the extract used indicating good wound healing properties. The amino acid profile reveals the presence of glycine, a proline that is important in wound healing. The E. coracana seeds, therefore, have wound healing properties based on the CAM model.

Formulation and Efficacy of Plaksha (Ficus lacor) Ointment in the Management of Non-Healing Ulcer

Chronic infected wound or non healing ulcer is commonly encountered problem faced in surgico-clinical cases. Wound healing property is most important pharmacological activity of Plaksh or Ficus lacor described by acharya Charaka and Bhavprakash, in an ancient compendia. A male patient aged 55 years, having non healing ulcer on right leg medially above the ankle joint due to trauma, reported to OPD. Wound was irregular with hyper keratinized edges, purulent discharge with foul smell, itching, redness and inflammation. Wound was cleaned with normal saline aseptically and ‘malhar’ or ointment which was prepared by using stem bark of Ficus lacor, was applied locally every day, for thirty days followed by alternate day for next fifteen days. ‘Malhar’ or ointment was prepared as per the formulation mentioned in ‘Ras tarangini’, an ancient compendia. Vitamin C, B complex and Zinc supplement was given orally. After forty five days, wound healed with healthy granulation tissue, purulent discharge and foul smell was totally absent .Bark contains phytochemicals like tannins, polyphenols caffiec acid. It possesses antioxident, anti-inflammatory, antibacterial, astringent activities. Caffeic acid have regulatory mechanism on glucose metabolism in diabetes.

PHARMACEUTICAL STUDY OF KANADI TAILA AND ITS MODIFIED OINTMENT

Aims: To prepare Kanadi Taila as per classical method and its modification into an ointment by using petroleum jelly as a base, as well as to do its organoleptic and physic-chemical analysis. Methods: Kanadi Taila was prepared as per the classical method of Taila Kalpana and its modification into an ointment by modern infusion method by using petroleum jelly as a base. Then it was evaluated for organoleptic and physic-chemical characteristics. Results: The final product Kanadi Taila and Kanadi Taila ointment were of desirable consistency and quality. Discussion: Kanadi Taila is well-known medicine for its wound healing property, and it was prepared as per classical method but due to its difficulty in application, there is a need to modify it into a more patient-friendly form. Kanadi Taila was modified into a topical ointment with white petroleum jelly as the base for attaining the desired consistency. Conclusion: The formulation Kanadi Taila is mentioned in the text Ayurveda Sara, Tratiya bhaga Prameha chikitsa adhyaya. Appropriate Paka Lakshanas were observed during Taila Paka, as well as the prepared Kanadi Taila ointment was of desired consistency

Wound healing potential of Launaea pinnatiϔida Cass leaf juice in rats

Launaea pinnatifida is a procumbent herb native to the coastal area and found throughout India and many other countries. It is utilized as a dietary plant in the preparation of various dishes in the Maldives. Traditionally it is used for the treatment of skin injuries and is reported to contain flavonoids, steroids, glycosides, tannins, saponins etc. As no scientific work has been found to be published related to wound healing potential of the L. pinnatifida plant, the present study investigated the effects of dried leaf juice of L. pinnatifida on wound healing property in rats using various wound models. In result analysis, the wound contraction percentage was found to be increased and the scar area and epithelization time were found to be decreased when 3 % ointment L. pinnatifida leaf juice was applied topically. Similarly, with incision and dead space wound models, wound breaking strength and hydroxyproline levels were found to be increased. So, Launaea pinnatifida leaf juice showed significant wound healing activity in all wound models, which supports its claim for being used traditionally in skin diseases.

Cytotoxicity and wound healing potential of jasmine essential oil

Jasminum sambac is an important fragrant and commercial flower crop of South India. Jasmine essential oil (JEO) is valued for its fragrance which is used mainly in the perfumery and cosmetic industry. JEO exhibits anti-inflammatory, antimicrobial activities and are used in the treatment of skin disorders. This present study was taken with the objective of understanding the cytotoxic and wound healing activity of JEO. JEO at different concentrations for cytotoxicity activity (0.5, 0.75, 1.0, 1.25 and 1.5 μg) and wound healing property (1.0, 1.25, 1.75 and 2.0 μg) were analyzed in-vitro for L929 skin cell lines. The results indicate that, JEO have moderate cytotoxic activity and lower wound healing property.

Efficacy of Plaksha (Ficus lacor) Ointment in the Management of Non- Healing Ulcer W.R.T. Dustavrana

ABSTRACT Chronic infected wound or non healing ulcer is a commonly encountered problem faced in surgical practice . wound healing property is most important pharmacological activity of Plaksh or Ficus lacor described by acharya Charaka and Bhavprakash . A 55 yrs old male patient of non healing ulcer having history of traumatic injury on right leg medially above the ankle joint .wound was irregular with hyper keratonized edges , purulent discharge with foul smell , itching , redness with inflammation. wound was cleaned with normal saline with aseptic precautions and malhar or ointment was used as external application for dressing on every day for one month and on alternate day for half month . Malhar or ointment was prepared on the basis of ‘Ras taranngini’, an ancient compendium . vitamin c, B complex and zinc supplement was given orally. At the end of one and half month wound was healed with healthy granulation tissue ,purulant discharge and foul smell was totally absent.

Measures aimed at prevention of cow’s mastitis incidence

In order to prevent mammary gland' disease, the specialists of InterChemmet LLC, to-gether with scientists from the St. Petersburg State Academy of Veterinary Medicine, have developed a hygiene product. "Compomol DS +" is used to treat the skin of the nipples of the udder before milking. It thoroughly and quickly cleanses the skin from various contaminants and helps to activate milk flow. Since the gate for the penetration of staphy-lococci and streptococci into the udder is an open nipple canal, foam treatment of each nipple was carried out. The nipple was dipped into a foaming glass with a 40% solu-tion of Compomol DS +. After that, the nip-ple was wiped with a soft napkin. Another product called Compomol DS Green Eco developed by InterChemmet LLC is applied to the udder nipples immediately after milking. The drug helps to normalize blood circulation after milking, has a wound healing property. The drug forms a mem-brane. This limits the access of microflora to the udder through the nipple, since the sphincter of the nipple canal remains open for 30 minutes after milking. When using «Compomol DS Green Eco» for 180 days, no negative effects on the skin of the nipples were observed. After hygienic treatment of the nipples, the animals be-haved calmly while putting on cups on the nipples and during milking, which contribut-ed to the complete extraction of milk from the mammary gland. The amount of residual milk after milking was not more than 40-60 ml of milk. An analysis of the results of the research indicates that the above hygiene products reduce the incidence of cows with mastitis by 33.4% and do not cause harmful effects to both animals and the staff. Using of hygiene products on the farm in 96 cows with-in 180 days of the experiment allowed to reduce economic damage by 39900 rubles. Measures aimed at prevention of cow’s mastitis incidence.