scholarly journals Urinary Biomarkers in the Assessment of Early Diabetic Nephropathy

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Cristina Gluhovschi ◽  
Gheorghe Gluhovschi ◽  
Ligia Petrica ◽  
Romulus Timar ◽  
Silvia Velciov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Early Diagnosis ◽  
Gold Standard ◽  
Tubular Injury ◽  
Inflammation Markers ◽  
Vascular Biomarkers ◽  
Early Diabetic Nephropathy ◽  
Biomarkers Of Oxidative Stress

Diabetic nephropathy (DN) is a frequent and severe complication of diabetes mellitus (DM). Its diagnosis in incipient stages may allow prompt interventions and an improved prognosis. Towards this aim, biomarkers for detecting early DN can be used. Microalbuminuria has been proven a remarkably useful biomarker, being used for diagnosis of DN, for assessing its associated condition—mainly cardiovascular ones—and for monitoring its progression. New researches are pointing that some of these biomarkers (i.e., glomerular, tubular, inflammation markers, and biomarkers of oxidative stress) precede albuminuria in some patients. However, their usefulness is widely debated in the literature and has not yet led to the validation of a new “gold standard” biomarker for the early diagnosis of DN. Currently, microalbuminuria is an important biomarker for both glomerular and tubular injury. Other glomerular biomarkers (transferrin and ceruloplasmin) are under evaluation. Tubular biomarkers in DN seem to be of a paramount importance in the early diagnosis of DN since tubular lesions occur early. Additionally, biomarkers of inflammation, oxidative stress, podocyte biomarkers, and vascular biomarkers have been employed for assessing early DN. The purpose of this review is to provide an overview of the current biomarkers used for the diagnosis of early DN.

scholarly journals The Significance of Urinary Markers in the Evaluation of Diabetic Nephropathy

2008 ◽  
Vol 27 (3) ◽  
pp. 376-382 ◽  
Author(s):  
Tatjana Cvetković ◽  
Predrag Vlahović ◽  
Vidosava đorđević ◽  
Lilika Zvezdanović ◽  
Dušica Pavlović ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Urine Concentration ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Urinary Markers ◽  
Stress Markers

The Significance of Urinary Markers in the Evaluation of Diabetic Nephropathy Oxidative stress is considered to be a unifying link between diabetes mellitus (DM) and its complications, including nephropathy (DN). The aim of this study was to determine the parameters of oxidative injury of lipids and proteins as well as the activity of ectoenzymes in the urine of DN patients. The study included 40 individuals: 10 patients with type 2 diabetes mellitus and microalbuminuria (DMT2-MIA), 10 type 2 diabetic patients with macroalbuminuria (DMT2-MAA), 10 patients with type 1 diabetes and microalbuminuria (DMT1-MIA) and 10 age- and sex-matched healthy subjects (control). In the urine we determined TBA reactive substances (TBARS), reactive carbonyl groups (RCG), and the activity of ectoenzymes N-acetyl-β-d-glucosaminidase (NAG), plasma cell differentiation antigen (PC-1), aminopeptidase N (APN) and dipeptidyl peptidase IV (DPP IV). A higher concentration of TBARS in the urine was found in DMT2-MIA and DMT1-MIA, compared to the control group (p<0.001 and P<0.05). The urine concentration of RCD shows similar results with a significant elevation in the groups with DMT2-MAA and DMT1-MIA, compared to the DMT2-MIA (p<0.001) and control group (p<0.001). Activities of NAG, APN and DPPIV were significantly higher in the urine of DMT2-MAA, compared to the control (p<0.01). The activity of PC-1 was slightly increased in that group, but not significantly. In conclusion, the level of oxidative stress markers and activities of brush border ectoenzymes in the urine may be a useful non-invasive and easily repeatable test in DN.

scholarly journals ASSESSMENT OF NEPHRIN AND PODOCIN LEVELS IN THE URINE OF PATIENTS WITH DIABETES MELLITUS

2017 ◽  
Vol 21 (2) ◽  
pp. 33-40
Author(s):  
I. N. Bobkova ◽  
A. A. Shchukina ◽  
M. V. Shestakova
Keyword(s):  
Diabetes Mellitus ◽  
Blood Pressure ◽  
Diabetic Nephropathy ◽  
Serum Creatinine ◽  
Arterial Hypertension ◽  
Healthy Subjects ◽  
Early Diagnostics ◽  
Urinary Levels ◽  
Patients With Diabetes ◽  
Early Diabetic Nephropathy

THE AIM:to assess excretion value of podocytes injury biomarkers in urine and to clarify their significance for early diabetic nephropathy (DN) diagnostics in diabetes mellitus (DM) patients with different severity of albuminuria (AU)/proteinuria(PU).PATIENTS AND METHODS.74 DM pts were studied, including 30 with type1 DM (T1DM) and 44 pts with type2 DM (T2DM). They were divided into three groups: 41 pts with AU <30 mg/gCr (A1), 13 pts with AU 30-300 mg/gCr (A2), 20 pts with PU (A3). CKD S1 was revealed in 41pts, CKD S2 – in 25 pts, CKD S3 – in 8 pts. Arterial hypertension was observed in 52 pts of 74(70%), mainly in T2DM. 10 healthy subjects were studied as control. Urinary levels of nephrin and podocin (an important slit diaphragm proteins) were measured by ELISA.RESULTS.Nephrinuria (NU) >5,84ng/ml/g, which not detecting in controls, was revealed in 63% of A1 pts, in 77% – in A2, in 80% – in A3. Podocinuria (PdU)>1,73ng/ml/g was revealed in 78% of A1 pts, in 54% of A2 and in 83% – A3. NU in pts with PU was significantly higher than in AU<30 mg/g. PDU in groups with different AU/PU was equally high and has no differ between DM types. Direct correlation was obtained between NU and AU (R=0,947 p<0,05). NU and PdU in T1DM correlated directly with serum creatinine (R=0,489 p<0,05 and R=0,468 p<0,05) and indirectly with GFR (R=-0,461 p<0,05 and R=-0,36 р<0,05). In DM duration less than 5 years NU directly correlated with НbА1с level, in T2DM – indirectly with systolic blood pressure.CONCLUSON. Nephrin and podocin levels can be useful for early diagnostics and monitoring of DN. 

Biomarkers of oxidative stress and endothelial dysfunction in glucose intolerance and diabetes mellitus

2008 ◽  
Vol 41 (18) ◽  
pp. 1454-1460 ◽  
Author(s):  
Edimar Cristiano Pereira ◽  
Simone Ferderbar ◽  
Marcelo Chiara Bertolami ◽  
André Arpad Faludi ◽  
Osmar Monte ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Endothelial Dysfunction ◽  
Glucose Intolerance ◽  
Biomarkers Of Oxidative Stress

Antioxidant status and biomarkers of oxidative stress in cats with diabetes mellitus

2009 ◽  
Vol 93 (2) ◽  
pp. 145-145
Author(s):  
J. R. Smith ◽  
S. K. Cox ◽  
S. D. Lauten ◽  
R. C. Hill ◽  
J. W. Bartges ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Antioxidant Status ◽  
Biomarkers Of Oxidative Stress

scholarly journals A Study on Oxidative stress and antioxidant status in Diabetic nephropathy

2020 ◽  
Vol 11 (SPL2) ◽  
pp. 113-115
Author(s):  
Gurupavan kumar Ganta1 ◽  
Kedari G S R
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
South Indian Population ◽  
Statistical Significant Difference ◽  
South Indian ◽  
Significant Difference ◽  
Anti Oxidant

Diabetes mellitus is the most common metabolic disorder in the world. The metabolic dysregulation of diabetes mellitus affects multiple organs like Kidneys, nerves, eyes and heart. Worldwide Among all diabetics, 20-40% of the people are suffering from Diabetic Nephropathy. Oxidative stress is increased by hyperglycemia in which Reactive Oxygen Species has been implicated in the pathology of these complications. The aim of our present study is one such attempt to find the role of oxidative stress by estimating the levels of oxidants and anti oxidant levels in diabetic nephropathy in ethnic south Indian population. 30 diagnosed cases of diabetic Nephropathy  were taken as cases and 30 age and sex matched Type 2 Diabetes mellitus patients were included as controls in the study. For oxidant levels Malondialdehyde (MDA) was estimated and for anti oxidant levels Super oxide Dismutase (SOD), Glutathione Peroxidase (GPx), Glutathione Reductase (GR) were measured. Statistically significant increase is observed in the levels of Malondialdehyde in cases when compared to controls. There is no statistical significant difference in the levels of Superoxide dismutase and Glutathione peroxidase between cases and controls. Whereas statistical significant difference is observed in the levels of Glutathione reductase in Diabetic Nephropathy cases when compared with controls.

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