Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique

Many pathologies of skin, especially ageing and cancer, involve modifications in the matrix alignment. Such tissue reorganization could have impact on cell behaviour and/or more global biological processes. Tissue engineering provides accurate study model by mimicking the skin and it allows the construction of versatile tridimensional models using human cells. It also avoids the use of animals, which gave sometimes nontranslatable results. Among the various techniques existing, the self-assembly method allows production of a near native skin, free of exogenous material. After cultivating human dermal fibroblasts in the presence of ascorbate during two weeks, a reseeding of these cells takes place after elevation of the resulting stroma on a permeable ring and culture pursued for another two weeks. This protocol induces a clear realignment of matrix fibres and cells parallel to the horizon. The thickness of this stretched reconstructed tissue is reduced compared to the stroma produced by the standard technique. Cell count is also reduced. In conclusion, a new, easy, and inexpensive method to produce aligned tissue free of exogenous material could be used for fundamental research applications in dermatology.

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Engineering Tissues without the Use of a Synthetic Scaffold: A Twenty-Year History of the Self-Assembly Method

BioMed Research International ◽

10.1155/2018/5684679 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 5

Author(s):

Ingrid Saba ◽

Weronika Jakubowska ◽

Stéphane Bolduc ◽

Stéphane Chabaud

Keyword(s):

Extracellular Matrix ◽

Self Assembly ◽

Large Scale ◽

Dermal Fibroblasts ◽

The Self ◽

Production Costs ◽

Scale Production ◽

Assembly Method ◽

Assembly Technique

Twenty years ago, Dr. François A. Auger, the founder of the Laboratory of Experimental Organogenesis (LOEX), introduced the self-assembly technique. This innovative technique relies on the ability of dermal fibroblasts to produce and assemble their own extracellular matrix, differing from all other tissue-engineering techniques that use preformed synthetic scaffolds. Nevertheless, the use of the self-assembly technique was limited for a long time due to its main drawbacks: time and cost. Recent scientific breakthroughs have addressed these limitations. New protocol modifications that aim at increasing the rate of extracellular matrix formation have been proposed to reduce the production costs and laboratory handling time of engineered tissues. Moreover, the introduction of vascularization strategies in vitro permits the formation of capillary-like networks within reconstructed tissues. These optimization strategies enable the large-scale production of inexpensive native-like substitutes using the self-assembly technique. These substitutes can be used to reconstruct three-dimensional models free of exogenous materials for clinical and fundamental applications.

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Humidity sensing properties of ZnO colloid crystals coated on quartz crystal microbalance by the self-assembly method

Ceramics International ◽

10.1016/j.ceramint.2012.10.190 ◽

2013 ◽

Vol 39 (4) ◽

pp. 3621-3625 ◽

Cited By ~ 16

Author(s):

Juan Xie ◽

Hu Wang ◽

Yuanhua Lin ◽

Ying Zhou ◽

Yuanpeng Wu

Keyword(s):

Quartz Crystal Microbalance ◽

Self Assembly ◽

Quartz Crystal ◽

The Self ◽

Humidity Sensing ◽

Sensing Properties ◽

Assembly Method ◽

Humidity Sensing Properties

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Electrochemical Behavior of a 4-Nitrothiophenol Modified Electrode Prepared by the Self-Assembly Method

Journal of Colloid and Interface Science ◽

10.1006/jcis.1995.1209 ◽

1995 ◽

Vol 171 (2) ◽

pp. 505-511 ◽

Cited By ~ 33

Author(s):

Hiromori Tsutsumi ◽

Shozo Furumoto ◽

Masayuki Morita ◽

Yoshiharu Matsuda

Keyword(s):

Modified Electrode ◽

Electrochemical Behavior ◽

Self Assembly ◽

The Self ◽

Assembly Method

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Shape-controlled synthesis of cubic-like selenium nanoparticles via the self-assembly method

Carbohydrate Polymers ◽

10.1016/j.carbpol.2016.08.004 ◽

2016 ◽

Vol 153 ◽

pp. 435-444 ◽

Cited By ~ 22

Author(s):

Urarika Luesakul ◽

Seamkwan Komenek ◽

Songchan Puthong ◽

Nongnuj Muangsin

Keyword(s):

Self Assembly ◽

The Self ◽

Selenium Nanoparticles ◽

Controlled Synthesis ◽

Assembly Method ◽

Shape Controlled

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Mastering nanostructured materials through H-bonding recognitions at interfaces

Pure and Applied Chemistry ◽

10.1351/pac-con-10-01-06 ◽

2010 ◽

Vol 82 (4) ◽

pp. 917-929 ◽

Cited By ~ 5

Author(s):

Stefan Mohnani ◽

Anna Llanes-Pallas ◽

Davide Bonifazi

Keyword(s):

Hydrogen Bonding ◽

Electronic Properties ◽

Nanostructured Materials ◽

Self Assembly ◽

The Self ◽

Memory Storage ◽

Technological Application ◽

Storage Devices ◽

Hydrogen Bonding Interactions ◽

Fundamental Research

The controlled engineering of functional architectures composed of π-systems with unusual opto-electronic properties is currently being investigated intensively from both fundamental research and technological application viewpoints. In particular, the exploitation of the supramolecular approach for the facile construction of multidimensional architectures, featuring cavities capable of hosting functional molecules, could be used in several applications, such as nanomedicine, molecular-based memory storage devices, and sensors. This paper highlights our recent strategies to use hydrogen-bonding interactions to prepare nanostructured functional architectures via the self-assembly of organic molecular modules studied at different interfaces.

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Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors

BioMed Research International ◽

10.1155/2020/6051210 ◽

2020 ◽

Vol 2020 ◽

pp. 1-23 ◽

Cited By ~ 1

Author(s):

Vincent Roy ◽

Brice Magne ◽

Maude Vaillancourt-Audet ◽

Mathieu Blais ◽

Stéphane Chabaud ◽

...

Keyword(s):

Tissue Engineering ◽

Tumor Microenvironment ◽

Self Assembly ◽

The Self ◽

Human Organ ◽

Assembly Method ◽

Cancer Models ◽

Organ Specific

Cancer research has considerably progressed with the improvement of in vitro study models, helping to understand the key role of the tumor microenvironment in cancer development and progression. Over the last few years, complex 3D human cell culture systems have gained much popularity over in vivo models, as they accurately mimic the tumor microenvironment and allow high-throughput drug screening. Of particular interest, in vitrohuman 3D tissue constructs, produced by the self-assembly method of tissue engineering, have been successfully used to model the tumor microenvironment and now represent a very promising approach to further develop diverse cancer models. In this review, we describe the importance of the tumor microenvironment and present the existing in vitro cancer models generated through the self-assembly method of tissue engineering. Lastly, we highlight the relevance of this approach to mimic various and complex tumors, including basal cell carcinoma, cutaneous neurofibroma, skin melanoma, bladder cancer, and uveal melanoma.

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New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique

Composition and Function of the Extracellular Matrix in the Human Body ◽

10.5772/62588 ◽

2016 ◽

Author(s):

Ingrid Saba ◽

Weronika Jakubowska ◽

Stéphane Chabaud ◽

Stéphane Bolduc

Keyword(s):

Tissue Engineering ◽

Self Assembly ◽

The Self ◽

Assembly Technique

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Photocatalytic Activity of Pd Doped Tin Dioxide Inverse Opal Films

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.534.135 ◽

2012 ◽

Vol 534 ◽

pp. 135-140 ◽

Cited By ~ 2

Author(s):

Yang Liu ◽

Bo Li ◽

You Wei Yao ◽

Jing Jing Gao ◽

Zhen Dong Liu ◽

...

Keyword(s):

Methylene Blue ◽

Photocatalytic Activity ◽

Self Assembly ◽

Tin Dioxide ◽

Uv Light ◽

Sol Gel ◽

The Self ◽

Inverse Opal ◽

Polystyrene Microsphere ◽

Assembly Technique

Inverse opal films (IOFs) of SnO2 doped with Pd were prepared by the self-assembly technique using polystyrene microsphere (PS sphere) as template in combination with a sol-gel method. The photocatalysis properties of SnO2 IOFs were estimated through measuring the rate of the degradation of methylene blue (MB). The result shows that SnO2 IOFs have good photocatalytic activity, the solution of MB was degradated over 60% in 4 hours when it was dipped in SnO2 IOFs and exposed in the UV light. The addition of Pd in SnO2 IOFs improved the photocatalytic activity of the films and the degradation of MB can exceed 80% with the same condition. This sort of SnO2 IOFs doped with Pd indicated a potential application in photocatalysis field.

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The Relationship between Growth Speed and Ambient Humidity in Convective Self-assembly

MRS Proceedings ◽

10.1557/proc-1273-mm01-07 ◽

2010 ◽

Vol 1273 ◽

Author(s):

Hans D. Robinson ◽

Kai Chen ◽

Stefan V. Stoianov

Keyword(s):

Growth Rate ◽

Self Assembly ◽

Standard Technique ◽

Crystal Growth Rate ◽

Growth Speed ◽

Linear Dependency ◽

Ambient Relative Humidity ◽

Wide Range ◽

Assembly Technique ◽

The Relationship

AbstractWe present a variation of a standard convective self-assembly technique, where the drying meniscus is restricted by a straight-edge located approximately 100 μm above the substrate adjacent to the drying zone. We find this technique to yield films at roughly twice the growth rate compared to the standard technique. We attribute this to differing local evaporation rates in the two cases. We also investigate how the crystal growth rate depends on ambient relative humidity and find a clear linear dependency, which we attribute to the length of the drying zone being constant over a wide range of humidities.

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