Osteogenic Differentiation of hDPSCs on Biogenic Bone Apatite Thin Films

A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is crucial for obtaining faster osseointegration compared to conventional, thick hydroxyapatite (HA) coatings, for both orthopedics and dentistry. Here, we investigated the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (hDPCS) on as-deposited and heat-treated BAp and stoichiometric HA. First, we showed that heat-treated BAp films can significantly promote hDPSC adhesion and proliferation. Moreover, hDPSCs, while initially maintaining the typical fibroblast-like morphology and stemness surface markers, later started expressing osteogenic markers such as Runx-2 and OSX. Noteworthy, when cultured in an osteogenic medium on annealed BAp films, hDPSCs were also able to reach a more mature and terminal commitment, with respect to HA and as-deposited films. Our findings suggest that annealed BAp films not only preserve the typical biological properties of stemness of, hDPSCs but also improve their ability of osteogenic commitment.

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PREPARATION AND STRUCTURE CHARACTERIZATION OF BiFeO3-SrBi2Nb2O9 THIN FILMS ON SILICON BY SOL-GEL PROCESSING

International Journal of Modern Physics B ◽

10.1142/s0217979202015601 ◽

2002 ◽

Vol 16 (28n29) ◽

pp. 4455-4459

Author(s):

HAOSHUANG GU ◽

WANQIANG CAO ◽

JUNMIN XUE ◽

JOHN WANG

Keyword(s):

Thin Films ◽

Sol Gel ◽

Precursor Solution ◽

Monomethyl Ether ◽

Structure Characterization ◽

Layered Perovskite ◽

Heat Treated ◽

Average Grain Size ◽

Gel Processing

A new system 0.1 BiFeO 3-0.9 SrBi 2 Nb 2 O 9 thin films have been successfully prepared by an ethanolamine-modified sol-gel technique. The precursor solution was synthesized from compounds, Bi(NO 3)3· 5H 2 O , Sr(NO 3)2, Fe(NO 3)3· 9H 2 O and Nb(OC 2 H 5)5 in solution ethylene glycol monomethyl ether. The thin films were deposited on Si single crystal by spinning coating, and heat-treated at temperatures ranging from 400°C to 700°C. Crystallization of thin films occurred at about 500 ~ 600°C and the films exhibit a pure phase of layered perovskite ferroelectric structure. The grain of films is well distributed and the average grain size of the film is about 100nm.

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Characterization of heat-treated tungsten thin films using picosecond duration thermoelastic transients

Optics and Lasers in Engineering ◽

10.1016/s0143-8166(02)00090-8 ◽

2003 ◽

Vol 40 (4) ◽

pp. 379-391 ◽

Cited By ~ 6

Author(s):

C.J.K. Richardson ◽

J.B. Spicer

Keyword(s):

Thin Films ◽

Heat Treated

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Structural Characterization of La1-xSrxCoO3 Thin Films Deposited by Pulsed Electron Deposition Method

Acta Physica Polonica A ◽

10.12693/aphyspola.130.1121 ◽

2016 ◽

Vol 130 (4) ◽

pp. 1121-1123

Author(s):

Ł. Cieniek ◽

A. Kopia ◽

A. Cyza ◽

K. Kowalski ◽

J. Kusiński

Keyword(s):

Thin Films ◽

Structural Characterization ◽

Deposition Method ◽

Pulsed Electron Deposition

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Preparation and Characterization of Tungsten Trioxide (WO3) Thin Films

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.678.32 ◽

2013 ◽

Vol 678 ◽

pp. 32-36 ◽

Cited By ~ 4

Author(s):

Raveendran Lavanya ◽

Gandhimathinathan Saroja ◽

Veerapandy Vasu

Keyword(s):

Thin Films ◽

Electron Beam Evaporation ◽

Glass Substrates ◽

Heat Treated ◽

Electron Beam Evaporation Technique ◽

Different Temperatures ◽

Evaporation Technique ◽

Crystalline Nature ◽

Wo3 Thin Films

The thin films of WO3were prepared on cleaned microscopic glass substrates by the electron beam evaporation technique. The films were coated at room temperature using pure WO3pellets as source. The prepared films were further post heat treated at different temperatures (100°C to 350°C) for about 1hr in air. The optical properties of WO3 thin films were studied in detail. The increase in the density of the film as the annealing temperature increases have been confirmed by the transmittance spectra. The film annealed at 250°C shows a strong photoluminescence peak. The peak intensity is found to be less for all other temperature. The observed results were discussed in terms of crystalline nature of WO3.

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Corrigendum to “Osteogenic Differentiation of hDPSCs on Biogenic Bone Apatite Thin Films”

Stem Cells International ◽

10.1155/2017/6587384 ◽

2017 ◽

Vol 2017 ◽

pp. 1-1 ◽

Cited By ~ 4

Author(s):

Michele Bianchi ◽

Alessandra Pisciotta ◽

Laura Bertoni ◽

Matteo Berni ◽

Alessandro Gambardella ◽

...

Keyword(s):

Thin Films ◽

Osteogenic Differentiation ◽

Bone Apatite

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Preparation and characterization of SrBi2Nb2O9 thin films made by polymeric precursors

Journal of Materials Research ◽

10.1557/jmr.1998.0400 ◽

1998 ◽

Vol 13 (10) ◽

pp. 2932-2935 ◽

Cited By ~ 21

Author(s):

S. M. Zanetti ◽

E. R. Leite ◽

E. Longo ◽

J. A. Varela

Keyword(s):

Thin Films ◽

Intermediate Phase ◽

Perovskite Phase ◽

Incident Angle ◽

Precursor Solution ◽

Fluorite Phase ◽

Polymeric Precursor ◽

Heat Treated ◽

Free Grazing

A polymeric precursor solution was employed in preparing SrBi2Nb2O9 (SBN) powder and thin films dip coated onto Si(100) substrate. XRD results show that the SBN perovskite phase forms at temperatures as low as 600 °C through an intermediate fluorite phase. This fluorite phase is observed for samples heat-treated at temperatures of 400 and 500 °C. After heat treatment at temperatures ranging from 300 to 800 °C, thin films were shown to be crack free. Grazing incident angle XRD characterization shows the occurrence of the fluorite intermediate phase for films also. The thickness of films, measured by MEV, was in the order of 80–100 nm.

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Preparation of Superconducting thin Films of High Tc Cuprates by a Novel Spin-on/Pyrolysis Technique

MRS Proceedings ◽

10.1557/proc-99-677 ◽

1987 ◽

Vol 99 ◽

Cited By ~ 1

Author(s):

C. E. Rice ◽

R. B. Van Dover ◽

G. J. Fisanick

Keyword(s):

Thin Films ◽

Scale Up ◽

Microstructural Characterization ◽

Room Temperature Resistivity ◽

Superconducting Thin Films ◽

Vacuum Equipment ◽

High Tc ◽

Heat Treated ◽

Compositional Uniformity

ABSTRACTSuperconducting thin films of high Tc cuprates have been prepared using wet chemical precursors, which are spun on a substrate, pyrolyzed and heat-treated. This new method is simple and inexpensive, and is easily amenable to scale-up to cover large areas. No vacuum equipment is required. The technique allows easy manipulation of film stoichiometry and gives films having excellent compositional uniformity. A 1600 A film of Ba2YCu3O7 prepared by this method had a room temperature resistivity of 500/xfzcm, Tc/(onset) of 90K, and R=0 at 58K. The preparation and electrical and microstructural characterization of thin films of Ba2YCu3O7 and other high TC cuprates will be discussed.

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