scholarly journals Effects of Diode Laser, Gaseous Ozone, and Medical Dressings onEnterococcus faecalisBiofilms in the Root Canal Ex Vivo

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Kerstin Bitter ◽  
Alexander Vlassakidis ◽  
Mediha Niepel ◽  
Daniela Hoedke ◽  
Julia Schulze ◽  
...  
Keyword(s):  
Diode Laser ◽  
Root Canal ◽  
Ex Vivo ◽  
Bacterial Reduction ◽  
Chi Square ◽  
Root Canals ◽  
Planktonic Bacteria ◽  
Gaseous Ozone ◽  
Colony Forming Units ◽  
Chi Square Test

The objective was to compare the antibacterial effects of adjunctive disinfection using diode laser and gaseous ozone compared to the medical dressings calcium hydroxide (Ca(OH)2) and chlorhexidine gel (CHX-Gel) onEnterococcus faecalisbiofilms in human root canals ex vivo. Root canals of 180 human extracted teeth were infected byE. faecalisand divided into 3 main groups (G): G1, control; G2, instrumentation and irrigation using 0.9% NaCl; G3, instrumentation and irrigation using 1% NaOCl. In each main group, the following treatments were applied: gaseous ozone, diode laser, and medical dressings of Ca(OH)2or CHX-Gel for 7 days (n=15). Reduction of colony forming units (CFUs) inside the root canal of planktons and frequencies of adherent bacteria after treatment were calculated. Bacterial reduction was significantly affected by the irrigation protocol (p<0.0005) and the disinfection method (p<0.0005), and a significant interaction between both factors could be observed (p<0.0005; ANOVA). In G3 (instrumentation using 1% NaOCl), no significant effect of disinfection methods could be demonstrated on planktonic bacteria (p=0.062; ANOVA) and frequencies of adherent bacteria (p>0.05; chi-square test). Instrumentation and irrigation using NaOCl combined with ozone or laser application resulted in comparable bacterial reduction onE. faecalisto the application of medical dressings.

scholarly journals Ex vivo accuracy of three electronic apex locators using different apical file sizes

2012 ◽  
Vol 23 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Bruno Carvalho de Vasconcelos ◽  
Leonardo de Alencar Matos ◽  
Elilton Cavalcante Pinheiro-Júnior ◽  
Antônio Sérgio Teixeira de Menezes ◽  
Nilton Vivacqua-Gomes
Keyword(s):  
Root Canal ◽  
Ex Vivo ◽  
Chi Square ◽  
Apical Constriction ◽  
Visual Measurement ◽  
Chi Square Test ◽  
The Mean

This study evaluated the accuracy of three electronic apex locators (Root ZX, Novapex, and Justy II) in root canal length determinations using different apical file sizes, considering the apical constriction (AC) and the major foramen (MF) as anatomic references. The diameter of the apical foramina of 40 single-rooted teeth was determined by direct visual measurement and the master apical file was established. Electronic measurements were then performed using 3 instruments: the selected master apical file (adjusted file), one size smaller (intermediate file), and two sizes smaller (misfit file). The distances from the tip of files fixed in the canals to the MF and to the AC were measured digitally. Precision at AC and at MF for the misfit, intermediate and adjusted apical files was as follows: 80%/88%/83% and 78%/83%/95% (Root ZX); 80%/85%/80% and 68%/73%/73% (Novapex); and 78%/80%/78% and 65%/78%/70% (Justy II). Considering the mean discrepancies, statistically significant differences were found only for the adjusted file at MF, with Root ZX presenting the best results at MF. The chi-square test showed significant differences between the acceptable measurements at AC and at MF for the Justy II and Novapex (± 0.5 mm) regardless of file adjustment. Under the conditions of the present study, all devices provided acceptable electronic measurements regardless of file adjustment, except for Root ZX which had its performance improved significantly when the precisely fit apical file was used. Justy II and Novapex provided electronic measurements nearest to the AC.

scholarly journals Analysis of anatomy and configuration of the canal system of the maxillary second premolar in the population of Bosnia and Herzegovina

2021 ◽  
Vol 12 (1) ◽  
pp. 9-18
Author(s):  
Brankica Davidović ◽  
Ljiljana Bjelović ◽  
Igor Radović ◽  
Bojana Davidović ◽  
Svjetlana Janković ◽  
...  
Keyword(s):  
Bosnia And Herzegovina ◽  
Root Canal ◽  
Statistical Significance ◽  
Type I ◽  
Canal System ◽  
Chi Square ◽  
Morphological Variations ◽  
Root Canals ◽  
Single Root ◽  
Chi Square Test

Introduction. Successful endodontic treatment depends upon the clinician's knowledge and ability to recognize and diagnose the presence of anatomical and morphological variations of the root and canal system. The aim of this study was to establish the number of roots and root canal configurations of the maxillary second premolar in the population of Bosnia and Herzegovina. Methods. The study sample was comprised of 150 maxillary second premolar teeth extracted for orthodontic or prosthetic reasons. Endodontic drills were used for trepanation of cavum dentis, and the number and patency of each root canal were determined by Kexpander # 15. Then, the samples were decalcified, made transparent and colored, to enable 3D viewing of the canal system. Decalcified teeth were observed from two projections (clinical and approximal) and analyzed in detail with a magnifying glass under 3 × and 5 × magnification in order to determine the number of roots, number of canals, root canal configuration using Vertucci's classification and number of anastomoses between canals. Statistical significance was obtained using Chi-square test. Results. The results obtained by decalcification of the teeth showed that, by radiographic analysis from the clinical projection, all the teeth had a single root. While, by the analysis from the approximal projection, 94.0% had one, 6.0% two roots. From the approximal projection, 70.7% with a single root canal and 29.3% with two root canals are visualized. The most common type of root canal configuration in the maxillary second premolars was Type I in both clinical (87.9%) and approximal projection (40.7%). Conclusion. These results emphasize the importance of knowing the variations in root canal morphology, because excluding the possibility of morphological variations can lead to failure of endodontic therapy

scholarly journals A comparative study of root canal shaping using protaper universal and protaper next rotary files in preclinical dental education

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7419 ◽  
Author(s):  
Gül Çelik ◽  
Feyza Özdemir Kısacık ◽  
Emir Faruk Yılmaz ◽  
Arife Mersinlioğlu ◽  
İhsan Furkan Ertuğrul ◽  
...  
Keyword(s):  
Root Canal ◽  
Dental Students ◽  
Chi Square ◽  
Significance Level ◽  
Root Canals ◽  
Rotary Instruments ◽  
Working Length ◽  
Chi Square Test ◽  
The Mean ◽  
Canal Shaping

BackgroundDentistry has undergone an evolution in endodontics practice caused by the advancement of rotary techniques for root canal preparation and their subsequent incorporation into the teaching of dentistry undergraduates. This research aimed to evaluate the shaping ability of third-year dental students as their first experience in rotary instrumentation using ProTaper Universal (PTU) and ProTaper Next (PTN) (Dentsply Maillefer) rotary instruments in simulated curved canals.MethodsForty students instrumented 200 simulated canals with a 40° curvature in resin blocks according to the manufacturer’s instructions with PTU and 39 students and 195 canals with PTN files. The canals were prepared at a speed of 300 rpm using a 16:1 reduction hand-piece powered by an electric motor (Xsmart; Dentsply Maillefer). The final apical preparation was set to F2 for the PTU and X2 for the PTN group. The change in canal curvature was evaluated based on Schneider technique using the AutoCAD 2007 software on post-digital photographs. The incidence of instrument fracture and deformation, the incidence of ledge, the change in working length (WL), and the working time were noted. The data were analyzed with Student’st-test and Chi-Square test at a significance level of 0.05 using SPSS.ResultsPTN maintained the original canal curvature better, resulting in fewer fractures and ledges, and shaped the canals faster than the PTU (P < 0.05). The mean curves of the resin canals after the instrumentation for the PTU and PTN groups were 24.03° ± 3.14° and 25.64° ± 2.72°, respectively. Thirty-three (17.4%) PTU and 18 (9.3%) PTN files fractured (p < 0.05). Nine (4.5%) PTU and 2 (2.6%) PTN deformed (p > 0.05). The change in WL after instrumentation was 0.97 mm ± 0.95 mm in PTU and 0.96 mm ± 0.80 mm in PTN (p < 0.05). The mean times were 627 s ± 18 s for PTU and 379 s ± 18 s for PTN (p < 0.000).DiscussionPTN can be recommended in severely curved root canals in terms of maintenance of the original canal curvature, superior instrument fracture and fewer ledges. Even if training before preparation provides an acceptable level of canal shaping for preclinical students, the use of NiTi rotary instruments should be included in the undergraduate dental curriculum, contributing to an increase in the quality of root canal shaping and, consequently, to an improvement of the clinical experience of students.

scholarly journals Variations in root canal morphology of the permanent mandibular first molar in Indian population- A CBCT study

2021 ◽  
Vol 7 (2) ◽  
pp. 61-66
Author(s):  
Vasavi Santosh ◽  
Rhea Reji John ◽  
Mandavi Waghmare ◽  
Reema Manoj
Keyword(s):  
Root Canal ◽  
Indian Population ◽  
Type I ◽  
Chi Square ◽  
Root Canals ◽  
Gender And Age ◽  
Chi Square Test ◽  
Root Canal Morphology ◽  
Mandibular First Molar ◽  
Canal Morphology

The purpose of this study was to determine the variations in the number of canals and its morphology in permanent mandibular first molars using cone-beam computed tomography (CBCT) in a subset of Indian population. A total of 350 CBCT scans were collected and evaluated in all three orthogonal planes to determine the number of root canals and study their anatomic variations using Vertucci’s classification.: The total number of roots and root canals, the root canal configuration, the incidence, and the correlations between left- and right-side occurrences and between males and females were analysed. Statistically significant differences were evaluated using the chi-square test with SPSS 16.0 for Windows (SPSS, Chicago, IL), with significance set at p &#60; 0.05.: The results of this study showed that predominantly, two thirds of the subjects had type VI mesial canal configuration and two-thirds had type I distal canal configuration. Also there was significant variance in the canal anatomy across gender and age of the subjects.: CBCT is a clinically useful tool in understanding the variations in root canal morphology, which guides in the assessment of the canal morphology aiding in successful root canal treatment.

scholarly journals Application of XP-Endo finisher files in the reduction of microbial load in oval-shaped root canals - An ex vivo study

2019 ◽  
Author(s):  
Giovanna Dornelas Mantovani ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Lidiane Mendes Louzada ◽  
Rodrigo Arruda Vasconcelos ◽  
Maria Cristina Carvalho ◽  
...  
Keyword(s):  
Saline Solution ◽  
Ex Vivo ◽  
Microbial Reduction ◽  
Bacterial Reduction ◽  
Significance Level ◽  
Root Canals ◽  
Colony Forming Units ◽  
Post Hoc ◽  
Cleaning Efficacy ◽  
Ex Vivo Study

The present study evaluated the effectiveness of XP-Endo Finisher (XPF) associated with XP-Endo Shaper (XPS) or Reciproc Blue (RB) files in the reduction of Enterococcus faecalis in oval-shaped root canals. Eighty mandibular incisors with single oval-shaped root canals were contaminated with E. faecalis for 10 days at 37°C with centrifugation on alternate days. The teeth were randomly assigned to eight experimental groups (n=10) as follows: G1 – XP-Endo Shaper, G2 – XP-Endo Shaper + XP-Endo Finisher, G3 – Reciproc Blue, and G4 – Reciproc Blue + XP-Endo Finisher. Chemomechanical preparation was performed with saline solution (NaCl) or 2.5% sodium hypochlorite (NaOCl). Microbial reduction was assessed by colony forming units (CFU/mL) count before (S1) and after (S2) CMP by using sterile paper points. ANOVA, Tukey’s test and Bonferroni’s post-hoc tests were used at 5% significance level. Bacteria were present in all initial samples (P>.05). All instrumentation techniques reduced bacteria, irrespective of the irrigating solution (P<.05). With the use of NaCl, Reciproc Blue was more effective than XP-Endo Shaper (P=.035). The association of XP-Endo Finisher improved the cleaning efficacy of both systems (P=.239). With the use of NaOCl, XP-Endo Shaper and Reciproc Blue presented similar effectiveness (P=.779). XP-Endo Finisher enhanced the bacterial reduction of both systems tested (P<.05). The use of NaOCl improved the CMP, irrespective of the instrumentation technique used (P<.05).

scholarly journals Extra canal in permanent maxillary first molar in patients undergoing root canal treatment at a tertiary care dental hospital, Nepal

2021 ◽  
Vol 10 (1) ◽  
pp. 47-51
Author(s):  
Archana Gharti ◽  
Neera Joshi ◽  
Kranti Prajapati ◽  
Sharada Devi Wagle ◽  
Suraj Shrestha
Keyword(s):  
Root Canal ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Root Canal Treatment ◽  
Chi Square ◽  
Cross Sectional ◽  
Root Canals ◽  
Conservative Dentistry ◽  
Ethical Approval ◽  
Chi Square Test

Background: Permanent maxillary first molar normally has three root canals but it can have extra root canals for which several studies were done in many parts of the globe. However, this type of study to explore extra canal is lagging in the context of Nepal. Objectives: The objective was to determine the percentage of extra canal in permanent maxillary first molar in patients undergoing root canal treatment and to compare the same between male and female. Methods: The descriptive cross-sectional study was conducted in the department of conservative dentistry and endodontics of People’s Dental College and Hospital from 20th December 2019 to 20th December 2020 after receiving ethical approval from Nepal Health Research Council on 18th December 2019 (ERB protocol No 888/2019) . One hundred and eighty permanent maxillary first molar teeth without root canal calcification were enrolled. Convenient sampling was done. Access opening was done and all canal orifices were located and recorded. Data were analyzed using Statistical Package for the Social Sciences (SPSS) version 22.0 with frequency table. Chi-square test was used for inferential statistics. Results: Out of 180 teeth, extra canals were present in 44(24.4%) having more numbers in male than in females. Extra canal was present in the mesiobuccal root. Conclusion: Extra canal in the permanent maxillary first molar is present in Nepalese population with greater number in male than in female. Thus, exploration of the pulp chamber in search of an extra canal is necessary to prevent failure of root canal treatment.

scholarly journals Effect of a Modified Irrigation Protocol on the Cleanliness of Moderately Curved Canals

2020 ◽  
Author(s):  
Garance Voegeli ◽  
Enrico Di Bella ◽  
Mustapha Mekki ◽  
Pierre Machtou ◽  
Serge Bouillaguet
Keyword(s):  
Root Canal ◽  
Apical Region ◽  
Chi Square ◽  
Root Canals ◽  
Chi Square Test ◽  
Root Dentin ◽  
Edx Analyses ◽  
Canal Shaping ◽  
Wallis Anova ◽  
Better Than

Abstract Objectives This study tested the hypothesis that modifying the sequence of sodium hypochlorite (NaOCl)/ethylene diamine tetra-acetic acid (EDTA) irrigation during root canal shaping would improve apical cleanliness in moderately curved canals. Materials and Methods Forty-five root canals were prepared using Protaper Gold with three irrigation protocols. Standard irrigation (SI) used 0.5 mL 3% NaOCl between each instrument, followed by 5 mL 17% EDTA manually agitated for 30 seconds. Reverse irrigation (RI) used 0.5 mL of 17% EDTA between each instrument, then 5 mL of 3% NaOCl, manually agitated for 30 seconds. Reverse irrigation plus (RI+) was similar to RI, except NaOCl (5 mL), used as a final rinse, (5 mL), used as a final rinse, was allowed to interact for 3 minutes with dentin before manual agitation (30 seconds).Root canal cleanliness was evaluated under the scanning electron microscope (SEM) (Hulsmann score); the chemical composition of dentin after irrigation was analyzed by energy dispersive X-ray (EDX). Statistical Analysis Results were compared using Kruskal–Wallis ANOVA by ranks and Wilcoxon matched paired posthoc tests. A Chi-square test assessed whether the best cleanliness would demonstrate a significant association with one irrigation protocol; odds ratio calculation was performed using score “1” versus score “2 or more” (2+) (p < 0.05). Results In the apical region, cleanliness was better in RI+ than SI and both significantly better than RI. Odd ratios indicate that the cleanliness in RI+ was significantly better than RI and SI groups (p < 0.000 and 0.003, respectively). Independently of the irrigation protocol, EDX analyses showed no chemical alteration of root dentin. Conclusions Using 17% EDTA during shaping, followed by 3% NaOCl rinse for 3 minutes, improved apical cleanliness without inducing erosion of dentin.

scholarly journals Effect of a 980-nm diode laser on post-operative pain after endodontic treatment in teeth with apical periodontitis: A randomized clinical trial

2020 ◽  
Author(s):  
Tuna Kaplan ◽  
Güzide Pelin Sezgin ◽  
Sema Sönmez Kaplan
Keyword(s):  
Diode Laser ◽  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Control Group ◽  
Pain Level ◽  
Chi Square ◽  
Root Canals ◽  
Post Operative Pain ◽  
Average Pain ◽  
Laser Group

Abstract Background: This study aimed to assess the effect of a 980-nm diode laser following chemomechanical root canal preparation on the severity of post-operative pain (PP) after root canal treatment (RCT). Methods: This study included asymptomatic, single-rooted teeth with a periapical index (PAI) score of 3 or 4. All the patients received RCT, including dressing with calcium hydroxide (Ca(OH) 2 ), during two visits. The patients were randomly divided into two groups ( n : 30): a control group, in which the final irrigation was performed using 5 ml of 2.5% sodium hypochlorite (NaOCI), followed by 5 ml of 17% and ethylenediaminetetraacetic acid (EDTA) and 5 ml of distilled water, and a laser group, in which the root canals were irradiated using a 980-nm diode laser after the final irrigation at both visits. The pain levels after both visits were evaluated using a visual analogue scale (VAS) after 8 h, 24 h, 48 h and 7 d. In addition, the time intervals to medication intake were recorded. The collected data were statistically analysed using the chi-square and Mann–Whitney U test ( p <0.05). Results: The average pain level in the control group 24 h after the first visit was significantly higher than that in the laser group ( p <0.05). The average pain level 24 h and 48 h after the second visit was significantly higher in the control group ( p <0.05). The levels of PP 24 h after the first visit were higher than those after the second visit only in the control group ( p <0.05). After the first visit, analgesic use in the control group was significantly higher after 8 h (40%) and 24 h (23%) as compared with that in the laser group ( p <0.05). Conclusions: Root canal irradiation with a diode laser may reduce PP after RCT in single-rooted teeth with a PAI score of 3 or 4.

scholarly journals Prevalence of major apical foramen mismatching the root apex in root canals of human permanent teeth

RSBO ◽  
2017 ◽  
Vol 13 (3) ◽  
pp. 188
Author(s):  
Liana Freire de Brito ◽  
Sylas Jamerson Sá Nogueira ◽  
Claudio Maniglia Ferreira ◽  
Fabio De Almeida Gomes ◽  
Bruno Carvalho Sousa
Keyword(s):  
Ex Vivo ◽  
Incidence Angle ◽  
Root Apex ◽  
Ethics Committee ◽  
Descriptive Statistics ◽  
Permanent Teeth ◽  
Chi Square ◽  
Root Canals ◽  
Working Length ◽  
Chi Square Test

The periapical radiograph is used to determine the working length in endodontic treatment. However, this radiograph does not define the position of the major apical foramen. Objective: This ex vivo laboratorial study aims to determine the prevalence of the major apical foramen mismatching the root apex in human permanent teeth, because when present, this mismatch makes the radiographic method inaccurate for determining the working length. Material and methods: This study used 400 human permanent teeth: 100 incisors, 100 canines, 100 premolars, and 100 molars, totalizing 634 canals (Ethics Committee, Federal University of Ceará #263/10). The access and negotiation of the root canals were performed with K-type files #10 and #15 until the tip of the instrument were visible at the root foramen through 5-fold magnifying glass. Periapical radiographs were performed on bucco-lingual incidence for single-rooted teeth; the incidence angle was changed for multi-rooted teeth. The films were processed and then analyzed by three calibrated examiners (Kappa 80%). Descriptive statistics and chi-square test were used for analyzing of the results. Results: The prevalence of major apical foramens mismatching the root apex was 19.72% of the canals studied. The incisors showed the lowest prevalence (9%, p<0.05) while the highest prevalence was seen in canine teeth (28%). Conclusion: According to the methodology applied, the prevalence of major apical foramens mismatching the root apex foramens make necessary an adjunct method for determining the working length, because it alone was not capable of determining the foramen position.

scholarly journals Dentinal Microcrack Formation During Root Canal Treatment with Mtwo Instruments and MicroSeal Filling Technique

2018 ◽  
Vol 9 (1-2) ◽  
pp. 31-37
Author(s):  
Teocrito Carlesi ◽  
Giuseppe Nigro ◽  
Luigi Di Giacomo ◽  
Gianluca Plotino ◽  
Vito Antonio Malagnino
Keyword(s):  
Root Canal ◽  
Root Canal Treatment ◽  
Control Group ◽  
Chi Square ◽  
Root Canal Filling ◽  
Root Canals ◽  
Rotary Instruments ◽  
Chi Square Test ◽  
Microcrack Formation ◽  
Group 2

Objective: The purpose of this study was to evaluate dentinal microcrack formation during root canal treatment using Mtwo NiTi rotary instruments and MicroSeal filling technique. Materials and Methods: Forty-five single-rooted teeth were selected for the present study and divided into 3 different groups ( n = 15): Group 1, in which root canals were instrumented with Mtwo rotary files; Group 2, in which root canals were instrumented with Mtwo rotary files and filled using the MicroSeal technique; and Group 3, that acts as a control group in which no instrumentation nor obturation were performed. Then, roots were sectioned 3 mm, 6 mm, and 9 mm from the apex and the cut surface has been observed under a stereomicroscope to check for the presence or absence of dentinal microcracks. Number of dentinal microcracks was registered for each group at different levels and data were statistically analyzed using the Chi-square test with a level of significance set at P < .05. Results: No statistically significant differences were registered among groups ( P < .05). No microcracks were detected in Groups 1 and 3, while two dentinal microcracks were registered in Group 2. Conclusions: Mtwo rotary instruments and the MicroSeal root canal filling technique did not significantly induce dentinal microcrack formation.

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