scholarly journals Identification of Dietzia Species in a Patient with Endophthalmitis following Penetrating Injury with Retained Intraocular Metallic Foreign Body

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Jesintha Navaratnam ◽  
Lumnije Dedi ◽  
Andreas Myklebust Tjølsen ◽  
Ragnheiður Bragadóttir
Keyword(s):  
Foreign Body ◽  
Deoxyribonucleic Acid ◽  
Flight Mass Spectrometry ◽  
First Case ◽  
Vitreous Sample ◽  
Ribosomal Ribonucleic Acid ◽  
Pars Plana ◽  
Polymerase Chain ◽  
Encoding Gene ◽  
Gram Positive Cocci

To the best of our knowledge, we report the first case of Dietzia species in a patient with endophthalmitis. A 47-year-old man presented to the ophthalmology department with decreased vision, redness, and minimal pain in his right eye after a foreign body struck his eye following working using a hammer and chisel. Broad-spectrum polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) sequencing targeting 16S ribosomal ribonucleic acid-(rRNA-) encoding gene on an undiluted vitreous sample revealed 100% identity with GenBank sequences of Dietzia species including D. natronolimnaea, D. dagingensis, and D. cercidiphylli. The culture of the vitreous samples demonstrated the growth of Gram-positive cocci and polymorphic rods. The isolate from the culture was identified as D. natronolimnaea using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The combination of surgical and medical treatment (pars plana vitrectomy and systemic and topical antibiotics) eradicated the infection successfully.

Transscleral Extraction of an Intraocular Foreign Body From the Posterior Segment of the Eye Without Pars Plana Vitrectomy

2020 ◽  
Vol 76 (1) ◽  
pp. 14-23
Author(s):  
Štěpán Rusňák ◽  
Lenka Hecová
Keyword(s):  
Foreign Body ◽  
Pars Plana Vitrectomy ◽  
Young Patients ◽  
Vitreous Body ◽  
Visual Control ◽  
Intraocular Foreign Body ◽  
Survey Study ◽  
Surgical Method ◽  
Eye Trauma ◽  
Pars Plana

Purpose: Penetrating eye trauma with an intraocular foreign body is very frequent, especially in men in their productive age. Pars plana vitrectomy would be the standard surgical method at our department. However, in indicated cases (metallic intraocular bodies in the posterior eye segment in young patients with well transparent ocular media without detached ZSM and without any evident vitreoretinal traction) transscleral extraction of the intraocular foreign body is performed using the exo magnet, eventually endo magnet with a minimal PPV without PVD induction under the visual control of endo-illumination. Materials and Methods: Between June 2003 and June 2018, 66 eyes of 66 patients diagnosed with a penetrating eye trauma caused by an intraocular foreign body located in the posterior eye segment were treated. In 18 eyes (27,3 %) with a metallic foreign body in vitreous (body) or in retina, no PPV or a minimal PPV without PVD was used as a surgical method. In the remaining 48 eyes (72,7 %), a standard 20G, respectively 23G PPV method were used together with PVD induction and the foreign body extraction via endo or exo magnet. Conclusions: As demonstrated by our survey/study, in the cases of a thoroughly considered indication an experimented vitreoretinal surgeon can perform a safe NCT transscleral extraction from the posterior eye segment via exo magnet, eventually endo magnet under the visual control of a contact display system with a minimal PPV. Thereby, the surgeon can enhance the patient´s chance to preserve their own lens and its accommodative abilities as well as reduce the risk of further surgical interventions of the afflicted eye.

The Importance of Fluconazole in Treatment of Endogenous Endophtalmitis in Patients Prior Treated Using Negative Pressure Therapy for Wound Closure Contaminated with Meticillin-resistant Staphylococcus aureus

2017 ◽  
Vol 68 (7) ◽  
pp. 1598-1601 ◽  
Author(s):  
Anisia Iuliana Alexa ◽  
Roxana Ciuntu ◽  
Alina Cantemir ◽  
Nicoleta Anton ◽  
Ciprian Danielescu ◽  
...  
Keyword(s):  
Antifungal Agents ◽  
Fungal Infections ◽  
Prolonged Treatment ◽  
Endogenous Endophthalmitis ◽  
Pressure Therapy ◽  
First Case ◽  
Severe Infections ◽  
Pars Plana ◽  
Delay In Treatment ◽  
Vitreous Biopsy

Severe infections with C. albicans should be treated promptly with antifungal agents, any delay in treatment increases the risk of endophthalmitis. The systemic Amphotericin B therapy is the gold standard in the treatment of endophthalmitis, but in the case of fungal infections it has not yet been determined. Numerous studies have shown that the use of Fluconazole is effective in the treatment of fungal endophthalmitis. In this paper, we report two cases (3 eyes) that have been presented for the same accusations of significant decrease of AV (visual acuity), ocular pain and blepharospasm suddenly installed, both of which required urgent antibiotic and intravenous antifungal treatment. Both are diagnosed with endogenous endophthalmitis and vitreous biopsy + VPP (pars plana vitrectomy) are performed, with a negative result of the vitreous culture. In both situations the treatment was with antibiotic and systemic antifungals. Postoperatively, evolution was favorable in the first case and less favorable in the second one. The prognosis depends on the virulence of the microorganisms and the time elapsed until initiation of the treatment. Also, the presence of risk factors such as diabetes, sepsis, recent abdominal surgery (C. Albicans is part of the comesary flora of the digestive tract) have influenced the prognosis decisively. Severe infections with C. albicans should be promptly treated with antifungal agents, any delay in treatment increases the risk of endophthalmitis. Even when prolonged treatment of candidemia is instituted, 3% of patients can develop endogenous endophthalmitis, so ocular evaluation is particularly important for patients immobilized in anesthesia and intensive care units.

scholarly journals Matrix-assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) as a Reliable Tool to Identify Species of Catalase-negative Gram-positive Cocci not Belonging to the Streptococcus Genus

2016 ◽  
Vol 10 (1) ◽  
pp. 202-208 ◽  
Author(s):  
Marisa Almuzara ◽  
Claudia Barberis ◽  
Viviana Rojas Velázquez ◽  
Maria Soledad Ramirez ◽  
Angela Famiglietti ◽  
...  
Keyword(s):  
Extraction Methods ◽  
Species Level ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Genus Level ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Level Identification ◽  
Gram Positive Cocci ◽  
Tof Ms

Objective:To evaluate the performance of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) by using 190 Catalase-negative Gram-Positive Cocci (GPC) clinical isolates.Methods:All isolates were identified by conventional phenotypic tests following the proposed scheme by Ruoff and Christensen and MALDI-TOF MS (Bruker Daltonics, BD, Bremen, Germany). Two different extraction methods (direct transfer formic acid method on spot and ethanol formic acid extraction method) and different cut-offs for genus/specie level identification were used. The score cut-offs recommended by the manufacturer (≥ 2.000 for species-level, 1.700 to 1.999 for genus level and <1.700 no reliable identification) and lower cut-off scores (≥1.500 for genus level, ≥ 1.700 for species-level and score <1.500 no reliable identification) were considered for identification. A minimum difference of 10% between the top and next closest score was required for a different genus or species.MALDI-TOF MS identification was considered correct when the result obtained from MS database agreed with the phenotypic identification result.When both methods gave discordant results, the 16S rDNA orsodAgenes sequencing was considered as the gold standard identification method. The results obtained by MS concordant with genes sequencing, although discordant with conventional phenotyping, were considered correct. MS results discordant with 16S orsodA identification were considered incorrect.Results:Using the score cut-offs recommended by the manufacturer, 97.37% and 81.05% were correctly identified to genus and species level, respectively. On the other hand, using lower cut-off scores for identification, 97.89% and 94.21% isolates were correctly identified to genus and species level respectively by MALDI-TOF MS and no significant differences between the results obtained with two extraction methods were obtained.Conclusion:The results obtained suggest that MALDI-TOF MS has the potential of being an accurate tool for Catalase-negative GPC identification even for those species with difficult diagnosis asHelcococcus,Abiotrophia,Granulicatella, among others. Nevertheless, expansion of the library, especially including more strains with different spectra on the same species might overcome potential “intraspecies” variability problems. Moreover, a decrease of the identification scores for species and genus-level identification must be considered since it may improve the MALDI-TOF MS accuracy.

scholarly journals Bartonella spp. in human and animal populations in Gauteng, South Africa, from 2007 to 2009

2012 ◽  
Vol 79 (2) ◽  
Author(s):  
Anastasia N. Trataris ◽  
Jennifer Rossouw ◽  
Lorraine Arntzen ◽  
Allan Karstaedt ◽  
John Frean
Keyword(s):  
Health Care Professionals ◽  
Deoxyribonucleic Acid ◽  
Bartonella Henselae ◽  
Hiv Positive ◽  
Host Immune System ◽  
Wild Rodent ◽  
Chain Reaction ◽  
Blood Samples ◽  
Animal Populations ◽  
Polymerase Chain

Bartonellae are highly adaptive organisms that have the ability to evade the host immune system and cause persistent bacteraemia by occupying the host’s erythrocytes. Bartonella spp. is under-studied and health care professionals often misdiagnose Bartonella-related infections. The aim of this study was to investigate the carriage of Bartonella spp. circulating in human and animal populations in Gauteng using culturing and polymerase chain reaction (PCR) detection. A total of 424 human, 98 cat, 179 dog, and 124 wild rodent blood samples were plated onto specialised media and incubated for 7–21 days at 37 ºC in CO2. Culture isolates morphologically similar to Bartonella control strains were confirmed by PCR and sequenced to determine species. Deoxyribonucleic acid (DNA) was extracted from all blood samples and tested by nested PCR. Bartonella could only be cultured from the cat and rodent specimens. Cat isolates were > 99% similar to Bartonella henselae URBHLIE 9, previously isolated from an endocarditis patient, and rat isolates were > 98% similar to either RN24BJ (candidus ‘Bartonella thailandensis’) or RN28BJ, previously isolated from rodents in China. The PCR prevalences were 22.5% in HIV-positive patients, 9.5% in clinically healthy volunteers, 23.5% in cats, 9% in dogs and 25% in rodents. Findings of this study have important implications for HIV-positive patients.

Vitrectorhexis in penetrating eye injuries in adults

2018 ◽  
Vol 29 (6) ◽  
pp. 689-693
Author(s):  
Miklós D Resch ◽  
Anikó Balogh ◽  
Gábor L Sándor ◽  
Zsuzsanna Géhl ◽  
Zoltán Zsolt Nagy
Keyword(s):  
Foreign Body ◽  
Pars Plana Vitrectomy ◽  
Anterior Segment ◽  
Posterior Chamber ◽  
Ocular Injury ◽  
Eye Injuries ◽  
Anterior Capsule ◽  
Safe Alternative ◽  
Anterior Lens Capsule ◽  
Pars Plana

Introduction: Vitrectorhexis is an alternative for manual continuous curvilinear capsulorhexis originally developed for paediatric cataract surgery. The aim of our study was the evaluation of the technique in adult patients with penetrating ocular injury and traumatic cataracts. Methods: Eight eyes of 8 patients (23–41 years, all males) had penetrating mechanical corneal trauma. Anterior lens capsule was penetrated in all cases and additional posterior capsule defect in five cases. Intraocular foreign body was detected in one case in the crystalline lens and in two cases in the posterior segment. Standard 23G infusion cannula and vitreous cutter were applied to perform anterior capsule opening, removal of lens material, anterior vitrectomy and pars plana vitrectomy if needed. No phacoemulsification or irrigation/aspiration probe was used. Results: Vitrectorhexis could be performed in 7 out of 8 cases with the preservation of peripheral anterior capsule; primary implantation of posterior chamber intraocular lens was possible in all cases (in the bag in three eyes and into the sulcus in five eyes). Anterior chamber was stable in all cases intraoperatively, and no dropped nucleus or lens fragment loss was observed. Surgery was combined with pars plana vitrectomy in three cases, with foreign body removal (when necessary). No postoperative complication occurred. Conclusion: Vitrectorhexis was found to be an effective and safe alternative method in the management of complex anterior segment trauma cases. With its use, traditional cataract surgical devices can be substituted and additional benefits of vitreous cutter can be utilized in selected cases, especially in young adults.

scholarly journals AI-Empowered Computational Examination of Chest Imaging for COVID-19 Treatment: A Review

2021 ◽  
Vol 4 ◽  
Author(s):  
Hanqiu Deng ◽  
Xingyu Li
Keyword(s):  
False Negative ◽  
Screening Tools ◽  
Polymerase Chain Reaction Test ◽  
Test Results ◽  
First Case ◽  
Lung Scan ◽  
Screening Efficiency ◽  
Polymerase Chain ◽  
Chain Reaction Test ◽  
Full Swing

Since the first case of coronavirus disease 2019 (COVID-19) was discovered in December 2019, COVID-19 swiftly spread over the world. By the end of March 2021, more than 136 million patients have been infected. Since the second and third waves of the COVID-19 outbreak are in full swing, investigating effective and timely solutions for patients’ check-ups and treatment is important. Although the SARS-CoV-2 virus-specific reverse transcription polymerase chain reaction test is recommended for the diagnosis of COVID-19, the test results are prone to be false negative in the early course of COVID-19 infection. To enhance the screening efficiency and accessibility, chest images captured via X-ray or computed tomography (CT) provide valuable information when evaluating patients with suspected COVID-19 infection. With advanced artificial intelligence (AI) techniques, AI-driven models training with lung scans emerge as quick diagnostic and screening tools for detecting COVID-19 infection in patients. In this article, we provide a comprehensive review of state-of-the-art AI-empowered methods for computational examination of COVID-19 patients with lung scans. In this regard, we searched for papers and preprints on bioRxiv, medRxiv, and arXiv published for the period from January 1, 2020, to March 31, 2021, using the keywords of COVID, lung scans, and AI. After the quality screening, 96 studies are included in this review. The reviewed studies were grouped into three categories based on their target application scenarios: automatic detection of coronavirus disease, infection segmentation, and severity assessment and prognosis prediction. The latest AI solutions to process and analyze chest images for COVID-19 treatment and their advantages and limitations are presented. In addition to reviewing the rapidly developing techniques, we also summarize publicly accessible lung scan image sets. The article ends with discussions of the challenges in current research and potential directions in designing effective computational solutions to fight against the COVID-19 pandemic in the future.

scholarly journals “COVID 19- Two Waves & More” What Have We Learnt?

2021 ◽  
Vol 5 (12) ◽  
Author(s):  
Manuj Kumar Sarkar ◽  
Subhra Dey ◽  
Boudhayan Das Munshi
Keyword(s):  
World Health Organisation ◽  
Physical Distance ◽  
World Health ◽  
Chain Reaction ◽  
Tissue Paper ◽  
Hand Sanitizer ◽  
First Case ◽  
The World ◽  
Polymerase Chain ◽  
Bat Coronavirus

The first case of SARS-CoV2 admitted on 26th December 2019 in Central Hospital, Wuhan, China. Broncho-alveolar lavage and Polymerase chain reaction of the aspirate showed high abundance of a viral RNA which has 89.1 % nucleotide identity with bat coronavirus previously isolated in China. Soon human to human transmission was observed and the outbreak started spreading. World Health Organisation on 11th March 2020 declared it as pandemic. COVID 19, caused by SARS-CoV-2, a disease we are still struggling to contain. With vaccination drive throughout the world, though the severity in re-infection has come down, but there is still threat by the various variants which are arising from time to time in various countries. The most effective way of preventing the spread of the virus is to keep physical distance from others of at least 1 meter, wearing a well fitted mask, keep hands clean and use hand sanitizer frequently, stay in well ventilated place, avoid crowded place and cough into bent elbow or tissue paper and get vaccinated when once’s turn comes. Therefore, we urge people to follow COVID appropriate behaviour properly. Keywords: COVID 19, SARS-CoV2, COVID appropriate behaviour, Social Distancing

scholarly journals First Report of a Case of Ocular Infection Caused by Purpureocillium lilacinum in Poland

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1046
Author(s):  
Robert Kuthan ◽  
Anna K. Kurowska ◽  
Justyna Izdebska ◽  
Jacek P. Szaflik ◽  
Anna Lutyńska ◽  
...  
Keyword(s):  
Contact Lens ◽  
Pars Plana Vitrectomy ◽  
Penetrating Keratoplasty ◽  
Visual Outcome ◽  
Ocular Infection ◽  
Corneal Perforation ◽  
Purpureocillium Lilacinum ◽  
First Case ◽  
Pars Plana ◽  
Tof Ms

This report describes the first case of an ocular infection induced by Purpureocillium lilacinum in Poland. The patient was a 51-year-old immunocompetent contact lens user who suffered from subacute keratitis and progressive granulomatous uveitis. He underwent penetrating keratoplasty for corneal perforation, followed by cataract surgery due to rapid uveitic cataract. A few weeks later, intraocular lens removal and pars plana vitrectomy were necessary due to endophthalmitis. The patient was treated with topical, systemic, and intravitreal voriconazole with improvement; however, the visual outcome was poor. The pathogen was identified by MALDI-TOF MS.

Sign in / Sign up

Export Citation Format

Share Document