The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood

Background. Matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) contribute to the inflammatory cascade in the cerebrospinal fluid (CSF) during bacterial meningitis. We determined levels of MPO, MMP-8, MMP-9, and tissue inhibitor of metalloproteinase- (TIMP-) 1 in the CSF of children with bacterial meningitis and investigated how these inflammatory mediators relate to each other and to the disease outcomes. Methods. Clinical data and the diagnostic CSF samples from 245 children (median age eight months) with bacterial meningitis were obtained from a clinical trial in Latin America in 1996–2003. MMP-9 levels in the CSF were assessed by zymography, while MMP-8, MPO, and TIMP-1 concentrations were determined with immunofluorometric and enzyme-linked immunosorbent assays. Results. MPO correlated positively with MMP-8 (rho 0.496, P<0.001) and MMP-9 (rho 0.153, P=0.02) but negatively with TIMP-1 (rho -0.361, P<0.001). MMP-8 emerged as the best predictor of disease outcomes: a CSF MMP-8 concentration above the median increased the odds of death 4.9-fold (95% confidence interval 1.8–12.9). Conclusions. CSF MMP-8 presented as an attractive prognostic marker in children with bacterial meningitis.

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Clinical Prognosis in Neonatal Bacterial Meningitis: The Role of Cerebrospinal Fluid Protein

PLoS ONE ◽

10.1371/journal.pone.0141620 ◽

2015 ◽

Vol 10 (10) ◽

pp. e0141620 ◽

Cited By ~ 12

Author(s):

Jintong Tan ◽

Juan Kan ◽

Gang Qiu ◽

Dongying Zhao ◽

Fang Ren ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Bacterial Meningitis ◽

Clinical Prognosis ◽

Cerebrospinal Fluid Protein

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Role of matrix metalloproteinases and tissue inhibitor of metalloproteinase in pathogenesis of patients with recurrent pregnancy loss

Journal of Reproductive Immunology ◽

10.1016/j.jri.2017.10.021 ◽

2017 ◽

Vol 124 ◽

pp. 78

Author(s):

Ryosuke Mori ◽

Fumiko Ozawa ◽

Yuki Obayashi ◽

Shinobu Goto ◽

Tamao Kitaori ◽

...

Keyword(s):

Matrix Metalloproteinases ◽

Pregnancy Loss ◽

Recurrent Pregnancy Loss ◽

Tissue Inhibitor Of Metalloproteinase ◽

Tissue Inhibitor

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Potential Role of Hyaluronic Acid on Bone in Osteoarthritis: Matrix Metalloproteinases, Aggrecanases, and RANKL Expression are Partially Prevented by Hyaluronic Acid in Interleukin 1-stimulated Osteoblasts

The Journal of Rheumatology ◽

10.3899/jrheum.130378 ◽

2014 ◽

Vol 41 (5) ◽

pp. 945-954 ◽

Cited By ~ 20

Author(s):

Zvezdana Mladenovic ◽

Anne-Sophie Saurel ◽

Francis Berenbaum ◽

Claire Jacques

Keyword(s):

Hyaluronic Acid ◽

Matrix Metalloproteinases ◽

Potential Role ◽

Proteolytic Enzymes ◽

Interleukin 1 ◽

Nuclear Factor Κb ◽

Mrna Levels ◽

Receptor Activator ◽

Polymerase Chain

Objective.To determine the effect of hyaluronic acid (HA) on proteolytic enzymes and bone remodeling mediators induced by interleukin 1β (IL-1β) and related to cartilage catabolism in murine osteoblasts.Methods.Osteoblasts were obtained from Swiss mice and cultured for 3 weeks. HA-treated osteoblasts were incubated with 100 μg/ml HA during the last week of culture, then stimulated with IL-1β (10 ng/ml) for 24 h. The expression of matrix metalloproteinases 3 and 13 (MMP-3 and MMP-13), ADAMTS-4 and ADAMTS-5, tissue inhibitor of metalloproteinases (TIMP), osteoprotegerin, and receptor activator of nuclear factor-κB ligand (RANKL) was determined by real-time polymerase chain reaction. MMP-3 and MMP-13 release was assessed by Western blot analysis.Results.IL-1β increased the mRNA levels of MMP-3 and MMP-13 and ADAMTS-4 and ADAMTS-5 and release of MMP-3 and MMP-13. Seven days of HA treatment significantly prevented the IL-1β-increased mRNA levels of MMP-3 (−61%, p < 0.01), MMP-13 (−56%, p < 0.01), ADAMTS-4 (−58%, p < 0.05), ADAMTS-5 (−52%, p < 0.01), and RANKL (−49%, p < 0.05), but not TIMP. As well, IL-1β-induced production of MMP-3 and MMP-13 was inhibited, by 27% (p < 0.01) and 40% (p < 0.01), respectively.Conclusion.In an inflammatory context in murine osteoblasts, HA can inhibit the expression of MMP and ADAMTS. Because HA can counteract the production of these mediators in chondrocytes, its beneficial effect in osteoarthritis may be due to its action on cartilage and subchondral bone.

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Potential role of the Virchow Robin space in the pathogenesis of bacterial meningitis

Medical Hypotheses ◽

10.1016/j.mehy.2017.09.014 ◽

2017 ◽

Vol 109 ◽

pp. 114-118 ◽

Cited By ~ 1

Author(s):

Patrick Chan ◽

Denis J. Meerdink ◽

James A. Uchizono

Keyword(s):

Bacterial Meningitis ◽

Potential Role ◽

Virchow Robin Space

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Potential role of leptin in angiogenesis: leptin induces endothelial cell proliferation and expression of matrix metalloproteinases in vivo and in vitro

Experimental & Molecular Medicine ◽

10.1038/emm.2001.17 ◽

2001 ◽

Vol 33 (2) ◽

pp. 95-102 ◽

Cited By ~ 284

Author(s):

Hyun-Young Park ◽

Hyuck Moon Kwon ◽

Hyun Joung Lim ◽

Bum Kee Hong ◽

Ju Yong Lee ◽

...

Keyword(s):

Cell Proliferation ◽

Endothelial Cell ◽

Matrix Metalloproteinases ◽

Potential Role ◽

Endothelial Cell Proliferation

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Virgin olive oil enriched with its own phenolic compounds or complemented with thyme improves endothelial function: The potential role of plasmatic fat-soluble vitamins. A double blind, randomized, controlled, cross-over clinical trial

Journal of Functional Foods ◽

10.1016/j.jff.2016.10.032 ◽

2017 ◽

Vol 28 ◽

pp. 285-292 ◽

Cited By ~ 6

Author(s):

Rosa-M. Valls ◽

Marta Farràs ◽

Anna Pedret ◽

Sara Fernández-Castillejo ◽

Úrsula Catalán ◽

...

Keyword(s):

Clinical Trial ◽

Phenolic Compounds ◽

Endothelial Function ◽

Olive Oil ◽

Potential Role ◽

Virgin Olive Oil ◽

Double Blind ◽

Randomized Controlled ◽

Fat Soluble Vitamins

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Comparison of enterovirus detection in cerebrospinal fluid with Bacterial Meningitis Score in children

Einstein (São Paulo) ◽

10.1590/s1679-45082017ao3880 ◽

2017 ◽

Vol 15 (2) ◽

pp. 167-172 ◽

Cited By ~ 4

Author(s):

Frederico Ribeiro Pires ◽

Andréia Christine Bonotto Farias Franco ◽

Alfredo Elias Gilio ◽

Eduardo Juan Troster

Keyword(s):

Cerebrospinal Fluid ◽

Bacterial Meningitis ◽

Aseptic Meningitis ◽

Medical Records ◽

Pediatric Patients ◽

Tertiary Hospital ◽

Viral Meningitis ◽

Parenteral Antibiotic ◽

Score Analysis

ABSTRACT Objective To measure the role of enterovirus detection in cerebrospinal fluid compared with the Bacterial Meningitis Score in children with meningitis. Methods A retrospective cohort based on analysis of medical records of pediatric patients diagnosed as meningitis, seen at a private and tertiary hospital in São Paulo, Brazil, between 2011 and 2014. Excluded were patients with critical illness, purpura, ventricular shunt or recent neurosurgery, immunosuppression, concomitant bacterial infection requiring parenteral antibiotic therapy, and those who received antibiotics 72 hours before lumbar puncture. Results The study included 503 patients. Sixty-four patients were excluded and 94 were not submitted to all tests for analysis. Of the remaining 345 patients, 7 were in the Bacterial Meningitis Group and 338 in the Aseptic Meningitis Group. There was no statistical difference between the groups. In the Bacterial Meningitis Score analysis, of the 338 patients with possible aseptic meningitis (negative cultures), 121 of them had one or more points in the Bacterial Meningitis Score, with sensitivity of 100%, specificity of 64.2%, and negative predictive value of 100%. Of the 121 patients with positive Bacterial Meningitis Score, 71% (86 patients) had a positive enterovirus detection in cerebrospinal fluid. Conclusion Enterovirus detection in cerebrospinal fluid was effective to differentiate bacterial from viral meningitis. When the test was analyzed together with the Bacterial Meningitis Score, specificity was higher when compared to Bacterial Meningitis Score alone.

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