scholarly journals Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Qianru Chi ◽  
Dongxu Wang ◽  
Xueyuan Hu ◽  
Shiping Li ◽  
Shu Li
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Sulfide ◽  
Energy Metabolism ◽  
Inflammatory Response ◽  
Human Health ◽  
Mapk Pathway ◽  
Classical Pathway ◽  
Inos Activity ◽  
Mda Content ◽  
Sulfur Containing

Hydrogen sulfide (H2S) is one of the main pollutants in the atmosphere, which is a serious threat to human health. The decomposition of sulfur-containing organics in chicken houses could produce a large amount of H2S, thereby damaging poultry health. In this study, one-day-old broilers were selected and exposed to 4 or 20 ppm of H2S gas (0-3 weeks: 4±0.5 ppm, 4-6 weeks: 20±0.5 ppm). The spleen samples were collected immediately after the chickens were euthanized at 2, 4, and 6 weeks. The histopathological and ultrastructural observations showed obvious necrosis characteristics of H2S-exposed spleens. H2S exposure suppressed GSH, CAT, T-AOC, and SOD activities; increased NO, H2O2, and MDA content and iNOS activity; and induced oxidative stress. ATPase activities and the expressions of energy metabolism-related genes were significantly decreased. Also, the expressions of related necroptosis (RIPK1, RIPK3, MLKL, TAK1, TAB2, and TAB3) were significantly increased, and the MAPK pathway was activated. Besides, H2S exposure activated the NF-κB classical pathway and induced TNF-α and IL-1β release. Taken together, we conclude that H2S exposure induces oxidative stress and energy metabolism dysfunction; evokes necroptosis; activates the MAPK pathway, eventually triggering the NF-κB pathway; and promotes inflammatory response in chicken spleens.

scholarly journals Hydrogen Sulfide Attenuates Aortic Remodeling in Aortic Dissection Associating with Moderated Inflammation and Oxidative Stress through a NO-Dependent Pathway

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 682
Author(s):  
Hsin-Ying Lu ◽  
Hung-Lung Hsu ◽  
Chih-Han Li ◽  
Shao-Jung Li ◽  
Shing-Jong Lin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Sulfide ◽  
Inflammatory Response ◽  
Aortic Dissection ◽  
Vascular Remodeling ◽  
Aortic Wall ◽  
Inflammatory Cells ◽  
Sod Activity ◽  
Aortic Remodeling ◽  
And Oxidative Stress

Aortic dissection (AD) is a highly lethal vascular disease characterized by separation of the constituent layers of the aortic wall. An increasing body of research indicates that inflammatory response and oxidative stress are implicated in vascular remodeling, which plays a key role in the development of AD. Hydrogen sulfide (H2S) has been found to protect against various types of cardiovascular disease, including myocardial infarction, arthrosclerosis, and hypertension. However, research on the effect of H2S on AD is insufficient. This study therefore elucidated the effect of H2S on the development and progression of AD, and the potential mechanism involved. Using β-aminopropionitrile fumarate (BAPN) and angiotensin II (Ang-II)-induced AD animal models, the administration of NaHS (as H2S donor, 56 μmol/kg body weight/day) was found to retard the development of AD. Murine VSMCs (Movas) exposed to interleukin-6 (IL-6) (20 ng/mL) to induce phenotypic switch. Histological analyses indicated that H2S administration inhibited the accumulation of inflammatory cells in the aortic wall and the related expression of inflammatory genes. Additionally, H2S treatment elevated aortic superoxide dismutase (SOD) activity and ablated malonaldehyde (MDA) and nitric oxide (NO) levels. In mechanistic terms, H2S attenuated IL-6 induced a pathological VSMC phenotypical switch through NO modulation by N(G)-monomethyl-L-arginine acetate salt (L-NMMA) stimulation. H2S inhibits AD formation by decreasing the inflammatory response, and oxidative stress, and by positively participating in vascular remodeling. These findings suggest a role for H2S as a novel and promising therapeutic strategy to prevent AD development.

Chinese Prescription Kangen-karyu and Salviae Miltiorrhizae Radix Improve Age-Related Oxidative Stress and Inflammatory Response through the PI3K/Akt or MAPK Pathways

2014 ◽  
Vol 42 (04) ◽  
pp. 987-1005 ◽  
Author(s):  
Chan Hum Park ◽  
Dae Hyun Kim ◽  
Min Hi Park ◽  
Mi Kyung Kim ◽  
Nam Deuk Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Response ◽  
Inflammatory Responses ◽  
Mapk Pathway ◽  
Mitogen Activated Protein Kinase ◽  
Salviae Miltiorrhizae ◽  
Mapk Pathways ◽  
Age Related ◽  
Salviae Miltiorrhizae Radix ◽  
Old Rats

This study examined whether Kangen-karyu and its crude drug, Salviae Miltiorrhizae Radix, have a reno-protective effect on the age-related oxidative stress and inflammatory response through the phosphoinositide 3-kinase (PI3K)/Akt or mitogen-activated protein kinase (MAPK) pathways in aged rats. Kangen-karyu or Salviae Miltiorrhizae Radix (20 mg/kg body weight/day) was administered orally to old groups of rats for 16 days, and their effects were compared with the vehicle-treated old and young rats. The administration of Kangen-karyu caused a slight decrease in the serum glucose level and a significant decrease in the serum insulin level in the old rats. The increased levels of serum renal functional parameter (urea-nitrogen) and oxidative parameter were significantly reduced by both Kangen-karyu and Salviae Miltiorrhizae Radix. The old rats exhibited a dysregulation of the protein expression related to insulin resistance, oxidative stress, and inflammation in the kidneys, but Kangen-karyu administration significantly reduced the expression of the inflammatory proteins through the PI3K/Akt pathway. On the other hand, the Salviae Miltiorrhizae Radix-treated old rats showed a decrease in the inflammatory cytokines through the MAPK pathway. These results provide important evidence that Kangen-karyu and Salviae Miltiorrhizae Radix have a pleiotropic effect on the PI3K/Akt and MAPK pathways, showing renoprotective effects against the development of inflammation in old rats. This study provides scientific evidence that Kangen-karyu and Salviae Miltiorrhizae Radix improve the inflammatory responses via the PI3K/Akt or MAPK pathways in the kidney of old rats.

scholarly journals Astaxanthin protects cognitive function of vascular dementia

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Ningwei Zhu ◽  
Xiao Liang ◽  
Ming Zhang ◽  
Xiaolan Yin ◽  
Hui Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cognitive Function ◽  
Inflammatory Response ◽  
Vascular Dementia ◽  
Morris Water Maze Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Sod Activity ◽  
Mda Content ◽  
And Oxidative Stress

Abstract Objective The purpose of this study was to evaluate the effect of astaxanthin (AST) on cognition function, inflammatory response and oxidative stress in vascular dementia (VD) mice. Method VD mice model was established by left unilateral common carotid arteries occlusion (LUCCAO). Following LUCCAO, AST was intragastrically administered for 30 days. Object recognition test and morris water maze test were used to evaluate cognitive function. Hematoxylin and eosin staining was performed to observe the hippocampal neuron structure. Enzyme-linked immunosorbent assay kit and bicinchoninic acid kit were respectively adopted to measure IL-1β and IL-4 protein expression and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in hippocampus and prefrontal cortex. Results AST improved the discrimination ability of VD mice. The escape latency and path length of VD mice treated with AST were dramatically reduced. Besides, AST 200 mg/kg enhanced crossing platform time and the number of times crossing the platform quadrant, and alleviated the morphological impairment in VD mice. Moreover, we found that AST inhibited IL-1β expression and MDA content, whereas promoted IL-4 expression and SOD activity in a dose-dependent manner. Conclusion AST could improve cognitive impairment and hippocampal neurons in VD mice, which may be related to suppression of inflammatory response and oxidative stress.

scholarly journals Effects of hydrogen sulfide on hemodynamics, inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats

Critical Care ◽  
2010 ◽  
Vol 14 (5) ◽  
Author(s):  
Frédérique Ganster ◽  
Mélanie Burban ◽  
Mathilde de la Bourdonnaye ◽  
Lionel Fizanne ◽  
Olivier Douay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Sulfide ◽  
Inflammatory Response ◽  
Hemorrhagic Shock ◽  
And Oxidative Stress

scholarly journals Hydrogen sulfide protects from cisplatin-induced acute kidney injury via attenuating inflammation activated by necroptosis in canine

2020 ◽  
Author(s):  
Shuang Wang ◽  
Enshuang Xu ◽  
Ruidong Ge ◽  
Haotian Jia ◽  
Yuying Fan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Kidney Injury ◽  
Hydrogen Sulfide ◽  
Energy Metabolism ◽  
Kidney Injury ◽  
The Body ◽  
Inflammatory Factors ◽  
Cancer Drug ◽  
Signal Molecules ◽  
Canine Kidney

Abstract Background Cisplatin, as an effective anti-cancer drug, has significant effects on a variety of solid tumors such as ovarian cancer and malignant lymphoma. However, cisplatin has strong nephrotoxicity, which greatly limits its clinical application. Unfortunately, the way to validly inhibit cisplatin-induced kidney damage remain poor understood currently. Hydrogen sulfide (H2S) was considered to be the third new gas signal molecules, involved in various physiological functions of the body. This present study was designed to investigate the effect of H2S on cisplatin-induced acute kidney injury (AKI) and the involved mechanisms in canine.Results Cisplatin-inject canine developed severe AKI symptom as indicated by renal dysfunction and pathological changes. Whereas H2S attenuated the serum creatinine (Scr), blood urea nitrogen (Bun) level and renal tubular damage. Cisplatin induced necroptosis and regulated the corresponding protein expression of RIPK1, RIPK3, PARP1, and then activated inflammatory factors expression such as TNF-α, NF-κB, IL-1β in canine kidney tissues. Cisplatin also triggered oxidative stress and affected energy metabolism. However, H2S significantly improved necroptosis and inflammation, manifested by increasing Cas8 activity and the expression of anti-inflammatory factors such as IL-4, IL-10 and IFN-γ. At the same time, the antioxidant capacity and energy metabolism levels of canine kidney were notably improved.Conclusion Collectively, our results suggest that H2S protect kidney from cisplatin-induced AKI via mitigating necroptosis and inflammation. Besides, H2S improved the level of energy metabolism and displayed a potency in cisplatin-induced oxidative stress. These provided a new idea for reducing cisplatin nephrotoxicity.

Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

2020 ◽  
Vol 11 (10) ◽  
pp. 8547-8559
Author(s):  
Hongjing Zhao ◽  
Yu Wang ◽  
Mengyao Mu ◽  
Menghao Guo ◽  
Hongxian Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Secondary Metabolites ◽  
Human Health ◽  
Grass Carp ◽  
Aquatic Environment ◽  
Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

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