A Simple Method for Patterning Nanoparticles on Planar Surfaces

This paper describes a simple method to pattern nanoparticles on planar surfaces using the antifouling property of poly(ethylene glycol) monolayers deposited from a solution on the native oxide of titanium. Atomic force microcopy was used to pattern the poly(ethylene glycol) monolayers producing protein active sites on the protein-resistant surface. Patterns with different sizes have been generated by shaving the monolayers with different repetitions. Friction force microscopy was used to image the patterns. The smallest patterns are 50 nm and the largest patterns are 500 nm at full width half maximum. The smallest pattern was produced with one shave, whereas the largest pattern was produced by shaving the monolayers 112 times. Protein-coated nanoparticles were immobilised on the shaved (protein active) part of the monolayers by dipping the patterned samples into a solution that contains 2% by volume protein-functionalized nanoparticles with a nominal diameter of 40 nm. Atomic force microscopy was used to take a topographic image of the samples. The topographic image showed that the protein-functionalized nanoparticles were attached onto the shaved part of the substrate but not on the poly(ethylene glycol)-covered part of the substrate. The level of aggregation of the nanoparticles was also investigated from the topographic image. The section analysis of the topographic image of the nanoparticle patterns showed a height of 40 nm which proved that only a monolayer of particles were deposited on the shaved part of the monolayer.