Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans

Anacardium occidentale (AO) contains a number of polyphenolic secondary metabolites with antioxidant activity. The objectives of this study were aimed at investigating the roles of AO leaf extracts in antioxidative stress and longevity, as well as their underlying mechanisms, in the Caenorhabditis elegans (C. elegans) model. AO extracts mediated the survival rate of nematodes under oxidative stress by attenuating intracellular reactive oxygen species (ROS) via the DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways. AO extracts stimulated the expression of stress response genes including SOD-3 and GST-4. Moreover, AO extracts exhibited antiaging activities and enhanced longevity. We observed improved pharyngeal pumping function, attenuation of pigment accumulation (lipofuscin), and an increased lifespan of the worms. Collectively, our results demonstrated that AO extracts exerted both oxidative stress resistance and antiaging properties in the C. elegans model and may lead to new agents to benefit humans in the near future.

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Gengnianchun, a Traditional Chinese Medicine, Enhances Oxidative Stress Resistance and Lifespan in Caenorhabditis elegans by Modulating daf-16/FOXO

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/8432306 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 8

Author(s):

Fanhui Meng ◽

Jun Li ◽

Wenjun Wang ◽

Yan Fu

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Stress Resistance ◽

Transgenic Animals ◽

Wild Type ◽

Oxidative Stress Resistance ◽

C Elegans ◽

And Oxidative Stress

Objective. Gengnianchun (GNC), a traditional Chinese medicine (TCM), is primarily used to improve declining functions related to aging. In this study, we investigated its prolongevity and stress resistance properties and explored the associated regulatory mechanism using a Caenorhabditis elegans model. Methods. Wild-type C. elegans N2 was used for lifespan analysis and oxidative stress resistance assays. Transgenic animals were used to investigate pathways associated with antioxidative stress activity. The effects of GNC on levels of reactive oxygen species (ROS) and expression of specific genes were examined. Results. GNC-treated wild-type worms showed an increase in survival time under both normal and oxidative stress conditions. GNC decreased intracellular ROS levels by 67.95%. GNC significantly enhanced the oxidative stress resistance of several mutant strains, suggesting that the protective effect of GNC is independent of the function of these genes. However, the oxidative stress resistance effect of GNC was absent in worms with daf-16 mutation. We also found upregulation of daf-16 downstream targets including sod-3 and mtl-1. Conclusions. Our findings suggest that GNC extends the lifespan of C. elegans and enhances its resistance to oxidative stress via a daf-16/FOXO-dependent pathway. This study also provides a feasible method for screening the biological mechanisms of TCMs.

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Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways

Phytomedicine ◽

10.1016/j.phymed.2019.153061 ◽

2019 ◽

Vol 64 ◽

pp. 153061 ◽

Cited By ~ 9

Author(s):

Chatrawee Duangjan ◽

Panthakarn Rangsinth ◽

Xiaojie Gu ◽

Shaoxiong Zhang ◽

Michael Wink ◽

...

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Signaling Pathways ◽

Stress Resistance ◽

Leaf Extracts ◽

Oxidative Stress Resistance ◽

And Oxidative Stress

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Streblus asper Lour. exerts MAPK and SKN-1 mediated anti-aging, anti-photoaging activities and imparts neuroprotection by ameliorating Aβ in Caenorhabditis elegans

Nutrition and Healthy Aging ◽

10.3233/nha-210121 ◽

2021 ◽

pp. 1-17

Author(s):

Mani Iyer Prasanth ◽

James Michael Brimson ◽

Dicson Sheeja Malar ◽

Anchalee Prasansuklab ◽

Tewin Tencomnao

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Stress Resistance ◽

Vital Role ◽

Transgenic Strain ◽

Wild Type ◽

Neuroprotective Effects ◽

C Elegans ◽

Streblus Asper

BACKGROUND: Streblus asper Lour., has been reported to have anti-aging and neuroprotective efficacies in vitro. OBJECTIVE: To analyze the anti-aging, anti-photoaging and neuroprotective efficacies of S. asper in Caenorhabditis elegans. METHODS: C. elegans (wild type and gene specific mutants) were treated with S. asper extract and analyzed for lifespan and other health benefits through physiological assays, fluorescence microscopy, qPCR and Western blot. RESULTS: The plant extract was found to increase the lifespan, reduce the accumulation of lipofuscin and modulate the expression of candidate genes. It could extend the lifespan of both daf-16 and daf-2 mutants whereas the pmk-1 mutant showed no effect. The activation of skn-1 was observed in skn-1::GFP transgenic strain and in qPCR expression. Further, the extract can extend the lifespan of UV-A exposed nematodes along with reducing ROS levels. Additionally, the extract also extends lifespan and reduces paralysis in Aβ transgenic strain, apart from reducing Aβ expression. CONCLUSIONS: S. asper was able to extend the lifespan and healthspan of C. elegans which was independent of DAF-16 pathway but dependent on SKN-1 and MAPK which could play a vital role in eliciting the anti-aging, anti-photoaging and neuroprotective effects, as the extract could impart oxidative stress resistance and neuroprotection.

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5′-Hydroxy-6, 7, 8, 3′, 4′-pentamethoxyflavone extends longevity mediated by DR-induced autophagy and oxidative stress resistance in C. elegans

GeroScience ◽

10.1007/s11357-020-00229-6 ◽

2020 ◽

Cited By ~ 1

Author(s):

Shalini Trivedi ◽

Rakesh Pandey

Keyword(s):

Oxidative Stress ◽

Stress Resistance ◽

Oxidative Stress Resistance ◽

C Elegans ◽

And Oxidative Stress

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Production of YP170 Vitellogenins Promotes Intestinal Senescence in Caenorhabditis elegans

The Journals of Gerontology Series A ◽

10.1093/gerona/glz067 ◽

2019 ◽

Vol 74 (8) ◽

pp. 1180-1188 ◽

Cited By ~ 2

Author(s):

Thanet Sornda ◽

Marina Ezcurra ◽

Carina Kern ◽

Evgeniy R Galimov ◽

Catherine Au ◽

...

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Life Span ◽

Stress Resistance ◽

Later Life ◽

Abundant Species ◽

Yolk Proteins ◽

Oxidative Stress Resistance ◽

Vitellogenin Synthesis ◽

Intestinal Atrophy

Abstract During aging, etiologies of senescence cause multiple pathologies, leading to morbidity and death. To understand aging requires identification of these etiologies. For example, Caenorhabditis elegans hermaphrodites consume their own intestinal biomass to support yolk production, which in later life drives intestinal atrophy and ectopic yolk deposition. Yolk proteins (YPs; vitellogenins) exist as three abundant species: YP170, derived from vit-1–vit-5; and YP115 and YP88, derived from vit-6. Here, we show that inhibiting YP170 synthesis leads to a reciprocal increase in YP115/YP88 levels and vice versa, an effect involving posttranscriptional mechanisms. Inhibiting YP170 production alone, despite increasing YP115/YP88 synthesis, reduces intestinal atrophy as much as inhibition of all YP synthesis, which increases life span. By contrast, inhibiting YP115/YP88 production alone accelerates intestinal atrophy and reduces life span, an effect that is dependent on increased YP170 production. Thus, despite copious abundance of both YP170 and YP115/YP88, only YP170 production is coupled to intestinal atrophy and shortened life span. In addition, increasing levels of YP115/YP88 but not of YP170 increases resistance to oxidative stress; thus, longevity resulting from reduced vitellogenin synthesis is not attributable to oxidative stress resistance.

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