Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies

The objectives of this study were to develop and optimize ultrasound-assisted extraction (UAE) for shikonin from Arnebia euchroma using response surface methodology (RSM) and to evaluate the antimicrobial activity of shikonin. The maximum yield of shikonin was 1.26% under the optimal extraction conditions (ultrasound power, 93 W; time, 87 min; temperature, 39°C; and liquid-solid ratio, 11 : 1). Shikonin showed inhibitory activity against standard strains and clinical isolates to varying extents (MICs ranging from 128 to 1024 μg/mL, MBCs ranging from 256 to 2048 μg/mL), and it was more effective for Gram-positive bacteria as indicated by lower MIC and MBC values. Time-kill curves revealed that antibacterial activity of shikonin exhibited a dose-response relationship. In summary, via this study, we identified ultrasound-assisted RSM as the optimal extraction method for shikonin, which is a potential material for the treatment of bacterial infections.

Download Full-text

A ULTRASOUND-ASSISTED EXTRACTION (UAE) OF PHYTOCHEMICALS WITH RESPONSE SURFACE METHODOLOGY(RSM) IN Curcuma xanthorrhiza

Science Proceedings Series ◽

10.31580/sps.v2i2.1431 ◽

2020 ◽

Vol 2 (2) ◽

pp. 139-146

Author(s):

Najiba Azemi ◽

Norazah Basar ◽

Susilawati Toemen

Keyword(s):

Response Surface Methodology ◽

Optimum Condition ◽

Response Surface ◽

Extraction Process ◽

Extraction Time ◽

Array Detector ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted

The Curcuma genus belongs to the Zingiberaceae family is commonly known for its usage as spice and medicine.it is widely used in pharmaceutical, food supplement, cosmetic industries lead to economic benefits. Recently, noticing to an eco-friendly extraction approach which provides the extraction efficiency is considered that in the extraction process, exploring the optimum condition of extraction parameters. This study is performed to improve the bioactive compounds extraction from C. xanthorrhiza by ultrasound-assisted extraction (UAE) through Response Surface Methodology (RSM). Three independent variables: extraction time (5-20 min), temperature (30-50 ° C) and liquid-solid ratio (6-10 mL / g) are applied using Box-Behnken (BBD) design. Quantification of curcumin and xanthorrhizol are carried out using reversed-phase high-performance liquid chromatography (RP HPLC) equiped by a photodiode array detector. Extraction time 20 minutes, temperature of 50°C, and LS ratio 8 mL/g are found as the optimum condition of extraction yield and quantification of xanthorrhizol. Curcumin 's optimum state, however, is observed at extraction time 12.50 minutes, temperature 30 ° C, and liquid-solid ratio of 10 mL / g. This study suggests that there are favorable potential using method of ultrasound-assisted extraction under specific parameters in the extraction process which is advantageous for advanced research.

Download Full-text

Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology

Journal of Food Quality ◽

10.1155/2021/5591022 ◽

2021 ◽

Vol 2021 ◽

pp. 1-14

Author(s):

Soumaya Hasni ◽

Ghayth Rigane ◽

Hanene Ghazghazi ◽

Hajer Riguene ◽

Amir Bouallegue ◽

...

Keyword(s):

Response Surface Methodology ◽

Phenolic Compounds ◽

Response Surface ◽

Total Phenolic ◽

Ultrasound Assisted Extraction ◽

Eucalyptus Marginata ◽

Esi Ms ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted

Eucalyptus marginata L. has a significant value in traditional medicine and recently has been shown to possess many pharmacological properties in vitro. The main goal of the present study was to optimize the extraction parameters of phenolic compounds from Eucalyptus marginata L. leaves using the extraction technique assisted by ultrasound in comparison with maceration using response surface methodology as a predicted tool. Therefore, total phenolic and flavonoid contents have been optimized, taking into account four variables: extraction time, temperature, liquid-to-solid ratio, and ethanol concentration. The optimum ultrasound-assisted extraction method for total phenolic and total flavonoid contents was obtained by ensuring the following parameters: t = 49.9 min, T = 74.9°C, liquid-to-solid ratio = 39.5 ml/g, and ethanol = 58.48%. The optimum extract has been subjected to LC-ESI-MS analysis. This technique allowed us to identify ten phenolic compounds: four phenolic acids mainly gallic acid (27.77 ± 0.06 µg/g DW) and protocatechuic acid (37.66 ± 0.04 µg/g DW) and six flavonoid compounds such as quercetrin (150.78 ± 0.02 µg/g DW) and hyperoside (39.19 ± 0.03 µg/g DW). These green and efficient procedures should be a promising option to guide industrial design for the production of phenolic-rich plant extracts.

Download Full-text

UPLC-ESI-MS/MS Analysis and Evaluation of Antioxidant Activity of Total Flavonoid Extract from Paeonia lactiflora Seed Peel and Optimization by Response Surface Methodology (RSM)

BioMed Research International ◽

10.1155/2021/7304107 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Guiqin Shi ◽

Jiaxin Shen ◽

Tao Wei ◽

Fei Ren ◽

Famou Guo ◽

...

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Response Surface ◽

Ethanol Concentration ◽

Paeonia Lactiflora ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasound Assisted

In this study, the ultrasound-assisted extraction (UAE) of flavonoid from Paeonia lactiflora seed peel was optimized by response surface methodology (RSM). Single-factor experiments and a three-factor three-level Box-Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction: ethanol concentration ( X 1 ), liquid-solid ratio ( X 2 ), and ultrasonic time ( X 3 ). The results showed that the optimal flavonoid yield (10.9045 mg RE/g) was as follows: ethanol concentration 62.93%, ultrasonic time 64.56 min, and liquid-solid ratio 24.86 mL/g. The optimized extract of P. lactiflora seed shell was further analyzed by UPLC-ESI-MS/MS, and 20 main flavonoids were identified and quantified, among which protocatechuic acid, vanillic acid, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzaldehyde had the highest content. Furthermore, the results of the antioxidant test showed that the P. lactiflora seed peel extract obtained under optimized UAE conditions exhibited good antioxidant activity. The experimental results showed that ultrasound-assisted extraction was a fast, efficient, and simple method for extracting active ingredients from P. lactiflora seed peel, thereby making this byproduct a promising source of compounds in food and healthcare sectors.

Download Full-text

Response Surface Methodology (RSM)-Based Optimization of Ultrasound-Assisted Extraction of Sennoside A, Sennoside B, Aloe-Emodin, Emodin, and Chrysophanol from Senna alexandrina (Aerial Parts): HPLC-UV and Antioxidant Analysis

Molecules ◽

10.3390/molecules27010298 ◽

2022 ◽

Vol 27 (1) ◽

pp. 298

Author(s):

Perwez Alam ◽

Omar M. Noman ◽

Rashed N. Herqash ◽

Omer M. Almarfadi ◽

Ali Akhtar ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Extraction Time ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Aerial Parts ◽

Assisted Extraction ◽

Aloe Emodin ◽

Ultrasound Assisted

In this study, ultrasound-assisted extraction conditions were optimized to maximize the yields of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol from S. alexandrina (aerial parts). The three UAE factors, extraction temperature (S1), extraction time (S2), and liquid to solid ratio (S3), were optimized using response surface methodology (RSM). A Box–Behnken design was used for experimental design and phytoconstituent analysis was performed using high-performance liquid chromatography-UV. The optimal extraction conditions were found to be a 64.2 °C extraction temperature, 52.1 min extraction time, and 25.2 mL/g liquid to solid ratio. The experimental values of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol (2.237, 12.792, 2.457, 0.261, and 1.529%, respectively) agreed with those predicted (2.152, 12.031, 2.331, 0.214, and 1.411%, respectively) by RSM models, thus demonstrating the appropriateness of the model used and the accomplishment of RSM in optimizing the extraction conditions. Excellent antioxidant properties were exhibited by S. alexandrina methanol extract obtained using the optimized extraction conditions with a DPPH assay (IC50 = 59.7 ± 1.93, µg/mL) and ABTS method (47.2 ± 1.40, µg/mL) compared to standard ascorbic acid.

Download Full-text

Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules ofPapaver somniferumby Response Surface Methodology

International Journal of Analytical Chemistry ◽

10.1155/2015/796349 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Ibrahim Bulduk ◽

Bahdışen Gezer ◽

Mustafa Cengiz

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Extraction Yield ◽

Quadratic Model ◽

Ultrasound Assisted Extraction ◽

Experimental Conditions ◽

Satisfactory Description ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted

In this study, amount of morphine from poppy capsules(Papaver somniferum)was investigated using ultrasonic assisted extraction (UAE). Response surface methodology was used to estimate effective experimental conditions on the content extraction of poppy capsules. For this purpose, solvent/solid ratio (10–20 mL/500 mg sample), pH (1–13), time (30–60 min), and temperature (30–50°C) were chosen as experimental variables. The affected response is extraction recovery values for morphine from poppy straw. For interpreting the relationship between experimental factors and response, a design table was established with combinations of three different concentrations levels of this compound in 29 trials. The second order quadratic model gave a satisfactory description of the experimental data. In our study,R-Squared (0.96), Adj-R-Squared (0.92), and PredR-Squared (0.78) values for extraction yield display good accuracy of the derived model. The predicted optimal conditions for the highest morphine level (3.38 mg morphine/500 mg-sample) were found at 19.99 mL solvent/500 mg solid ratio, 59.94 min extraction time, 1.10 pH, and 42.36°C temperature. In the optimal extraction conditions, the experimental values are very close to the predicted values. Consequently, the response surface modeling can be achieved sufficiently to predict extraction yield from poppy straw by ultrasound assisted extraction.

Download Full-text