A ULTRASOUND-ASSISTED EXTRACTION (UAE) OF PHYTOCHEMICALS WITH RESPONSE SURFACE METHODOLOGY(RSM) IN Curcuma xanthorrhiza

2020 ◽  
Vol 2 (2) ◽  
pp. 139-146
Author(s):  
Najiba Azemi ◽  
Norazah Basar ◽  
Susilawati Toemen
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Response Surface ◽  
Extraction Process ◽  
Extraction Time ◽  
Array Detector ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The Curcuma genus belongs to the Zingiberaceae family is commonly known for its usage as spice and medicine.it is widely used in pharmaceutical, food supplement, cosmetic industries lead to economic benefits. Recently, noticing to an eco-friendly extraction approach which provides the extraction efficiency is considered that in the extraction process, exploring the optimum condition of extraction parameters. This study is performed to improve the bioactive compounds extraction from C. xanthorrhiza by ultrasound-assisted extraction (UAE) through Response Surface Methodology (RSM). Three independent variables: extraction time (5-20 min), temperature (30-50 ° C) and liquid-solid ratio (6-10 mL / g) are applied using Box-Behnken (BBD) design. Quantification of curcumin and xanthorrhizol are carried out using reversed-phase high-performance liquid chromatography (RP HPLC) equiped by a photodiode array detector. Extraction time 20 minutes, temperature of 50°C, and LS ratio 8 mL/g are found as the optimum condition of extraction yield and quantification of xanthorrhizol. Curcumin 's optimum state, however, is observed at extraction time 12.50 minutes, temperature 30 ° C, and liquid-solid ratio of 10 mL / g. This study suggests that there are favorable potential using method of ultrasound-assisted extraction under specific parameters in the extraction process which is advantageous for advanced research.  

scholarly journals Response Surface Methodology (RSM)-Based Optimization of Ultrasound-Assisted Extraction of Sennoside A, Sennoside B, Aloe-Emodin, Emodin, and Chrysophanol from Senna alexandrina (Aerial Parts): HPLC-UV and Antioxidant Analysis

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 298
Author(s):  
Perwez Alam ◽  
Omar M. Noman ◽  
Rashed N. Herqash ◽  
Omer M. Almarfadi ◽  
Ali Akhtar ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Aerial Parts ◽  
Assisted Extraction ◽  
Aloe Emodin ◽  
Ultrasound Assisted

In this study, ultrasound-assisted extraction conditions were optimized to maximize the yields of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol from S. alexandrina (aerial parts). The three UAE factors, extraction temperature (S1), extraction time (S2), and liquid to solid ratio (S3), were optimized using response surface methodology (RSM). A Box–Behnken design was used for experimental design and phytoconstituent analysis was performed using high-performance liquid chromatography-UV. The optimal extraction conditions were found to be a 64.2 °C extraction temperature, 52.1 min extraction time, and 25.2 mL/g liquid to solid ratio. The experimental values of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol (2.237, 12.792, 2.457, 0.261, and 1.529%, respectively) agreed with those predicted (2.152, 12.031, 2.331, 0.214, and 1.411%, respectively) by RSM models, thus demonstrating the appropriateness of the model used and the accomplishment of RSM in optimizing the extraction conditions. Excellent antioxidant properties were exhibited by S. alexandrina methanol extract obtained using the optimized extraction conditions with a DPPH assay (IC50 = 59.7 ± 1.93, µg/mL) and ABTS method (47.2 ± 1.40, µg/mL) compared to standard ascorbic acid.

scholarly journals Modelling and Optimization of Ultrasound Assisted Extraction of Polyphenols Using Response Surface Methodology

2020 ◽  
Vol 5 (9) ◽  
pp. 1004-1012
Author(s):  
Junior Franck Ekorong Akouan Anta ◽  
Biloa Dorothée Marcelle ◽  
Bruno Fabrice Siewe ◽  
Raghavarao K. S. M. S.
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Reducing Power ◽  
Extraction Yield ◽  
Operating Conditions ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Stirring Rate ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Mango seed kernels are by-products of the consumption and transformation of mango fruits (Mangifera indica L.). Many ways of valorisation have been proposed, and among them, their phenolic compounds extraction. To increase the extraction yield, ultrasound-assisted extraction was modelled and optimized. The 4 factors Central Composite design associated with the Response Surface Methodology (RSM) were used to achieve that goal. The effect of extraction time, temperature, stirring rate and the Ultrasound Amplitude, on the total phenolic compound extraction yield and the total reducing power of the extract, were studied and modelled. The modelling allows us to do a multi-response optimization to identify the best-operating conditions to achieve at the same time the highest extraction yield and antioxidant capacity. The optimal operating conditions achieved were 41.82 min of extraction time, 54.75⁰C as extraction time, under 266.67 rpm as stirring rate, and 100% ultrasound amplitude. With an expected extraction yield of 71.35 mg GA/g, and 123.058 mg AA/g of total reducing power. 2 extraction cycles, under these conditions, are enough to extract a maximum of the phenolic content, under the described conditions.

scholarly journals Ultrasound-Assisted Extraction for Simultaneous Quantitation of Potential Sweetening Compounds from Derris reticulata Aqueous Extracts: A Response Surface Methodology Approach

2020 ◽  
Author(s):  
Keerati Thamapan ◽  
Natta Laohakunjit ◽  
Orapin Kerdchuchen ◽  
Punchira Vongsawasdi ◽  
Withawat Mingvanish
Keyword(s):  
Response Surface Methodology ◽  
Optimal Condition ◽  
Response Surface ◽  
Glycyrrhetinic Acid ◽  
Array Detector ◽  
Aqueous Extracts ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Derris Reticulata

Abstract Background: Derris reticulata or Oi Sam Saun is a highly sweet Thai plant, rich in bioactive compounds, and widely used for its medicinal properties. In this study, sweet aqueous extracts from the stems of Oi Sam Saun were extracted using ultrasound-assisted extraction (UAE). Phenolic, flavonoid, and sugar compound extraction was optimized using the Box-Behnken design (BBD) and response surface methodology (RSM). Methods: Three independent variables—extraction temperature (40–80°C), sonication time (20–60 min), and extraction ratio (1:10–1:30 g/mL)—were investigated, and the optimal condition was used to determined phenolics, flavonoid and 18β-glycyrrhetinic acid by High performance liquid chromatography-diode array detector (HPLC-DAD). Sensory evaluation was also performed.Results: The values of 84°C, 64 min, and ratio 1:8 g/mL were found to be optimal. Under these conditions, experimental values were well correlated with predicted values, and phenolic, flavonoid, and sugar contents were determined as 0.4725 mgGAE/gDW, 0.1489 ± 0.033 mgCE/gDW, and 4.802 ± 0.651 mg/gDW, respectively. Gallic acid, p-coumaric acid, quercetin, and kaempferol was also found in optimal condition. Moreover, the extract contained 18β-glycyrrhetinic acid (0.529 ± 0.002 mg/100 mg) and was 166 times sweeter than sucrose.Conclusion: High level of phenolics, flavonoids and sugars was detected in optimal condition of the extract. Therefore, this Thai medicinal plant, which has several pharmacological benefits, is newly potentially and applicable as a sweetening agent or sugar substitute in foods.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Bioactive Compounds from Acacia Seyal Gum Using Response Surface Methodology and Their Chemical Content Identification by Raman, FTIR, and GC-TOFMS

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1612
Author(s):  
Tahani Maher ◽  
Nassereldeen A. Kabbashi ◽  
Mohamed E. S. Mirghani ◽  
Md Z. Alam ◽  
Djabir Daddiouaissa ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Bioactive Compounds ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Acacia Seyal

Acacia Seyal gum (ASG), also known as gum Arabic, is an antioxidant-rich soluble fiber. ASG has been reported to have many biological activities, including anticancer, antidiabetic, antiulcer, and immunomodulatory activity. Extraction of bioactive compounds from ASG is commonly performed using conventional extraction methods. However, these techniques have certain limitation in terms of extraction time, energy, and solvent requirements. Ultrasound-assisted extraction (UAE) could be used as an alternative technique to extract bioactive compounds in less time, at low temperature, and with less energy and solvent requirements. In this study, the UAE extraction of ASG was optimized using response surface methodology (RSM). A face-centered central composite design (FCCCD) was used to monitor the effect of different independent factors of ultrasound operation (sonication time, temperature, and solvent ratio) on ASG extraction yield. In addition, screening and characterization of phytochemicals in 60% ethanol ASG extract was carried out using Raman microscopy, Fourier transform infrared spectroscopy (FTIR), and gas chromatography time-of-flight mass spectroscopy (GC-TOFMS) analysis. The results indicated that, under optimal conditions (extraction time 45 min, extraction temperature 40 °C, and solid–liquid ratio of 1:25 g/mL), the yield of ASG was 75.87% ± 0.10. This yield was reasonably close to the predicted yield of 75.39% suggested by the design of experiment. The ANOVA revealed that the model was highly significant due to the low probability value (p < 0.0001). Raman spectrum fingerprint detected polysaccharides, such as galactose and glucose, and protein like lysine and proline, while FTIR spectrum revealed the presence of functional groups peaks value of alkanes, aldehydes, aliphatic amines, and phenol. GC-TOFMS spectroscopic detected the presence of strong d-galactopyranose, carotenoid, and lycopene antioxidant compounds. In conclusion, this study demonstrated that the UAE technique is an efficient method to achieve a high yield of ASG extracts. The selected model is adequate to optimize the extraction of several chemical compounds reported in this study.

scholarly journals Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Soumaya Hasni ◽  
Ghayth Rigane ◽  
Hanene Ghazghazi ◽  
Hajer Riguene ◽  
Amir Bouallegue ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Eucalyptus Marginata ◽  
Esi Ms ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Eucalyptus marginata L. has a significant value in traditional medicine and recently has been shown to possess many pharmacological properties in vitro. The main goal of the present study was to optimize the extraction parameters of phenolic compounds from Eucalyptus marginata L. leaves using the extraction technique assisted by ultrasound in comparison with maceration using response surface methodology as a predicted tool. Therefore, total phenolic and flavonoid contents have been optimized, taking into account four variables: extraction time, temperature, liquid-to-solid ratio, and ethanol concentration. The optimum ultrasound-assisted extraction method for total phenolic and total flavonoid contents was obtained by ensuring the following parameters: t = 49.9 min, T = 74.9°C, liquid-to-solid ratio = 39.5 ml/g, and ethanol = 58.48%. The optimum extract has been subjected to LC-ESI-MS analysis. This technique allowed us to identify ten phenolic compounds: four phenolic acids mainly gallic acid (27.77 ± 0.06 µg/g DW) and protocatechuic acid (37.66 ± 0.04 µg/g DW) and six flavonoid compounds such as quercetrin (150.78 ± 0.02 µg/g DW) and hyperoside (39.19 ± 0.03 µg/g DW). These green and efficient procedures should be a promising option to guide industrial design for the production of phenolic-rich plant extracts.

scholarly journals Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Xiang-Yue Huang ◽  
Hua-Lin Fu ◽  
Hua-Qiao Tang ◽  
Zhong-Qiong Yin ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Bacterial Infections ◽  
Maximum Yield ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Arnebia Euchroma ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Time Kill

The objectives of this study were to develop and optimize ultrasound-assisted extraction (UAE) for shikonin from Arnebia euchroma using response surface methodology (RSM) and to evaluate the antimicrobial activity of shikonin. The maximum yield of shikonin was 1.26% under the optimal extraction conditions (ultrasound power, 93 W; time, 87 min; temperature, 39°C; and liquid-solid ratio, 11 : 1). Shikonin showed inhibitory activity against standard strains and clinical isolates to varying extents (MICs ranging from 128 to 1024 μg/mL, MBCs ranging from 256 to 2048 μg/mL), and it was more effective for Gram-positive bacteria as indicated by lower MIC and MBC values. Time-kill curves revealed that antibacterial activity of shikonin exhibited a dose-response relationship. In summary, via this study, we identified ultrasound-assisted RSM as the optimal extraction method for shikonin, which is a potential material for the treatment of bacterial infections.

scholarly journals Ultrasound-Assisted Extraction of Carotenoids from Carrot Pomace and Their Optimization through Response Surface Methodology

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6763
Author(s):  
Muhammad Umair ◽  
Saqib Jabbar ◽  
Mustapha M. Nasiru ◽  
Zhaoxin Lu ◽  
Jianhao Zhang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Total Carotenoids ◽  
Independent Variables ◽  
Assisted Extraction ◽  
Productive Process ◽  
Ultrasound Assisted

Ultrasound-assisted extraction (UAE) was used to extract carotenoids from the carrot pomace. To investigate the effect of independent variables on the UAE, the response surface methodology (RSM) with central-composite design (CCD) was employed. The study was conducted with three independent variables including extraction time (min), temperature (°C), and ethanol concentration (%). The results showed that the optimal conditions for UAE were achieved with an extraction time of 17 min, temperature of 32 °C, and ethanol concentration of 51% of total carotenoids (31.82 ± 0.55); extraction time of 16 min, temperature of 29 °C, and ethanol concentration of 59% for a combination of β-carotene (14.89 ± 0.40), lutein (5.77 ± 0.19), and lycopene (2.65 ± 0.12). The non-significant (p > 0.05) correlation under optimal extraction conditions between predicted and experimental values suggested that UAE is the more productive process than conventional techniques for the extraction of carotenoids from the carrot pomace.

scholarly journals Optimization of Ultrasound Assisted Extraction of Isoliquiritigenin from Licorice by Response Surface Methodology

2015 ◽  
Vol 9 (1) ◽  
pp. 149-154
Author(s):  
Hu Zhiyong ◽  
Zhou Yajing ◽  
Xu Xia ◽  
Liu Shuyan ◽  
Jing Jie
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Chemopreventive Agent ◽  
Assisted Extraction ◽  
Potential Cancer

Isoliquiritigenin (ILG) of licorice is a potential cancer chemopreventive agent. In this paper, response surface methodology (RSM) integrating with Box-Behnken design (BBD) was firstly employed to optimize the ILG extraction, which based on the single-factor experiments. According to analysis, the effect of extraction time and ethanol concentration on yields was significantly. The optimum values were the ethanol concentration of 73.25%, extraction time of 27.82 min and liquid-solid ratio of 19.10: 1. Under these conditions, the highest yield of ILG from licorice was 0.26 mg/g.

scholarly journals UPLC-ESI-MS/MS Analysis and Evaluation of Antioxidant Activity of Total Flavonoid Extract from Paeonia lactiflora Seed Peel and Optimization by Response Surface Methodology (RSM)

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Guiqin Shi ◽  
Jiaxin Shen ◽  
Tao Wei ◽  
Fei Ren ◽  
Famou Guo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Paeonia Lactiflora ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasound Assisted

In this study, the ultrasound-assisted extraction (UAE) of flavonoid from Paeonia lactiflora seed peel was optimized by response surface methodology (RSM). Single-factor experiments and a three-factor three-level Box-Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction: ethanol concentration ( X 1 ), liquid-solid ratio ( X 2 ), and ultrasonic time ( X 3 ). The results showed that the optimal flavonoid yield (10.9045 mg RE/g) was as follows: ethanol concentration 62.93%, ultrasonic time 64.56 min, and liquid-solid ratio 24.86 mL/g. The optimized extract of P. lactiflora seed shell was further analyzed by UPLC-ESI-MS/MS, and 20 main flavonoids were identified and quantified, among which protocatechuic acid, vanillic acid, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzaldehyde had the highest content. Furthermore, the results of the antioxidant test showed that the P. lactiflora seed peel extract obtained under optimized UAE conditions exhibited good antioxidant activity. The experimental results showed that ultrasound-assisted extraction was a fast, efficient, and simple method for extracting active ingredients from P. lactiflora seed peel, thereby making this byproduct a promising source of compounds in food and healthcare sectors.

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