Cytokine and Chemokine Profile in Patients with Multiple Myeloma Treated with Bortezomib

The aim of the study was to determine the levels of selected cytokines and chemokines in the serum of multiple myeloma (MM) patients treated with bortezomib-based regimens. A total of 71 MM patients were examined: 41 with primary refractory disease (17) or early relapse (28), and 30 who were bortezomib sensitive with no progression for at least six months. Patients who demonstrated CR or PR after bortezomib-based therapies longer than six months after treatment discontinuation were designated bortezomib sensitive. Serum cytokine levels were assayed with Bio-Rad Bio-Plex Pro Human Cytokine 27-Plex Assay on the MAGPIX Multiplex Reader and the Bio-Plex® 200 System (Bio-Rad). Higher levels of MIP-1α and lower levels of MIP-1β and IL-9 were associated with better responses to bortezomib-based treatment, and higher levels of IL-1ra and IL-8 were associated with bone involvement. MCP-1 was elevated in patients with hemoglobin<10 g/dl compared to those without anemia. The levels of IL-8, MIP-1α, and TNF-α were significantly higher in patients with renal insufficiency. Only MIP-1α was elevated in patients with hypercalcemia compared to patients with normal calcium levels. In conclusion, distinct cytokines are involved in the pathogenesis of MM and may play a prominent role in the prediction of treatment response. However, a single measurement of serum cytokines should be interpreted with caution and further studies are needed.

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Serum Cytokine Levels in Patients of Multiple Myeloma at Diagnosis and at Relapse

Clinical Lymphoma Myeloma & Leukemia ◽

10.1016/j.clml.2015.07.535 ◽

2015 ◽

Vol 15 ◽

pp. e252

Author(s):

S. Jasrotia ◽

L. Kumar ◽

R. Gupta

Keyword(s):

Multiple Myeloma ◽

Serum Cytokine ◽

Cytokine Levels

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Effect of peanut oil and nandrolone on serum cytokines in pubertal term rats

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.7819 ◽

2017 ◽

Author(s):

Erdal Tasgin ◽

Seyfullah Haliloglu

Keyword(s):

Serum Levels ◽

Peanut Oil ◽

Male Rats ◽

Control Group ◽

Nandrolone Decanoate ◽

Serum Cytokine ◽

Blood Samples ◽

Serum Cytokines ◽

Cytokine Levels ◽

Cytokine Synthesis

The purpose of this study was to determine the effect of nandrolone on cytokine levels in pubertal term female and male rats. Totally 60 pubertal term Spraque Dawley rat (30/30 female/male) were divided into 3 equal groups. Control group received no treatment. PO group received intraperitoneally peanut oil every 5 days with an intervening gap of 2 days where no treatment was given. ND group received intraperitoneally nandrolone decanoate (10 mg/kg) within peanut oil like PO group. At the end of the four weeks, blood samples were obtained. Serum cytokine levels were measured by ELISA. Peanut oil significantly (P less than 0.05) increased serum levels of interleukin-1â and interleukin-6 in pubertal term male rats, while nandrolone decanoate had no any effect on the cytokine levels compared to experimental groups. It may be stated that nandrolone decanoate has no excessive inducer effect on the cytokine synthesis in pubertal male and female term.

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Serum Cytokine and Chemokine Profile in Relation to the Severity of Coronavirus Disease 2019 in China

The Journal of Infectious Diseases ◽

10.1093/infdis/jiaa363 ◽

2020 ◽

Vol 222 (5) ◽

pp. 746-754 ◽

Cited By ~ 22

Author(s):

Ying Chi ◽

Yiyue Ge ◽

Bin Wu ◽

Wenshuai Zhang ◽

Tao Wu ◽

...

Keyword(s):

Infectious Disease ◽

Proinflammatory Cytokine ◽

Serum Levels ◽

Therapeutic Strategies ◽

Clinical Classification ◽

Serum Cytokine ◽

Infected Male ◽

Chemokine Production ◽

Serum Cytokines ◽

Cytokines And Chemokines

Abstract Coronavirus disease 2019 (COVID-19) is an emerging infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We investigated the serum cytokine and chemokine levels in asymptomatic, mild, moderate, severe, and convalescent SARS-CoV-2–infected cases. Proinflammatory cytokine and chemokine production induced by SARS-CoV-2 were observed not only in symptomatic patients but also in asymptomatic cases, and returned to normal after recovery. IL-6, IL-7, IL-10, IL-18, G-CSF, M-CSF, MCP-1, MCP-3, IP-10, MIG, and MIP-1α were found to be associated with the severity of COVID-19. Moreover, a set of cytokine and chemokine profiles were significantly higher in SARS-CoV-2–infected male than female patients. The serum levels of MCP-1, G-CSF, and VEGF were weakly and positively correlated with viral titers. We suggest that combinatorial analysis of serum cytokines and chemokines with clinical classification may contribute to evaluation of the severity of COVID-19 and optimize the therapeutic strategies.

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Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay

Scientific Reports ◽

10.1038/s41598-021-81953-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Laura Hocum Stone ◽

Scott Hunter Oppler ◽

Julia L. Nugent ◽

Sarah Gresch ◽

Bernhard J. Hering ◽

...

Keyword(s):

Immune Responses ◽

Nonhuman Primates ◽

Rhesus Macaques ◽

Clinical Settings ◽

Model Design ◽

Serum Cytokine ◽

Serum Cytokines ◽

Cytokine Levels ◽

The Impact ◽

Cytokine Profiling

AbstractCytokine profiling is a valuable tool for monitoring immune responses associated with disease and treatment. This study assessed the impact of sex and sedation on serum cytokines in healthy nonhuman primates (NHPs). Twenty-three cytokines were measured from serum using a bead-based multiplex assay. Assay validation for precision, sensitivity, recovery, linearity, and stability was performed. Samples from male and female cynomolgus and rhesus macaques either cooperating or sedated were compared. All cytokines except TNFα demonstrated acceptable sensitivity and precision, with variable recovery and linearity. IFNγ, IL-2, IL-5, IL-6, IL-8, IL-12/23 (p40), IL-13, IL-15, MCP-1, TGFα, VEGF met acceptance criteria; G-CSF, IL-4, IL-10, MIP1α, sCD40L were marginal. Higher cytokine levels were observed in females and cytokine levels were blunted in sedated NHPs when compared to awake cooperating NHPs. Significant differences observed in cytokines related to sex, species, or imposed by handling highlight the importance of model design on translational relevance for clinical settings.

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Serum cytokine levels in congenital diaphragmatic hernia: predictive impact for the need for extracorporeal membrane oxygenation?

Klinische Pädiatrie ◽

10.1055/s-0030-1261313 ◽

2010 ◽

Vol 222 (S 01) ◽

Author(s):

M Veit ◽

E Tutdibi ◽

J Tautz ◽

T Schaible ◽

L Gortner

Keyword(s):

Extracorporeal Membrane Oxygenation ◽

Congenital Diaphragmatic Hernia ◽

Diaphragmatic Hernia ◽

Serum Cytokine ◽

Membrane Oxygenation ◽

Cytokine Levels

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An Examination of Generalized Pustular Psoriasis in Pregnancy Based on the Serum Cytokine Levels.

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.61.144 ◽

1999 ◽

Vol 61 (2) ◽

pp. 144-147

Author(s):

Miyako KUDO ◽

Yoshihiro UMEBAYASHI ◽

Tomoe KOTSUJI ◽

Fujio OTSUKA

Keyword(s):

Pustular Psoriasis ◽

Serum Cytokine ◽

Cytokine Levels ◽

Generalized Pustular Psoriasis ◽

In Pregnancy

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Associations of serum cytokines and chemokines with the risk of incident cancer in a prospective rheumatoid arthritis cohort

International Immunopharmacology ◽

10.1016/j.intimp.2021.107719 ◽

2021 ◽

Vol 97 ◽

pp. 107719

Author(s):

Bryant R. England ◽

Megan Campany ◽

Harlan Sayles ◽

Punyasha Roul ◽

Yangyuna Yang ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Serum Cytokines ◽

Cytokines And Chemokines ◽

Rheumatoid Arthritis Cohort ◽

Incident Cancer

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Reactive arthritis and undifferentiated peripheral spondyloarthritis share human leucocyte antigen B27 subtypes and serum and synovial fluid cytokine profiles

Rheumatology ◽

10.1093/rheumatology/keaa746 ◽

2020 ◽

Author(s):

Jyoti Ranjan Parida ◽

Sandeep Kumar ◽

Sakir Ahmed ◽

Smriti Chaurasia ◽

Ratnadeep Mukherjee ◽

...

Keyword(s):

Statistical Difference ◽

Human Leucocyte Antigen ◽

Healthy Controls ◽

Hla B27 ◽

Serum Cytokine ◽

Cytokine Profiles ◽

Cytokine Levels ◽

Peripheral Spondyloarthritis ◽

Multiplex Cytokine ◽

Age And Sex

Abstract Objectives Peripheral SpA (pSpA) is comprised of ReA, PsA, enteritis-associated arthritis and undifferentiated pSpA (upSpA). ReA and upSpA share T cell oligotypes and metabolomics in serum and SF. We investigated HLA-B27 subtypes and cytokines in serum and SF that were compared between ReA and upSpA. Methods ReA and upSpA were compared in two cohorts. In cohort I (44 ReA and 56 upSpA), HLA-B27 subtyping was carried out. In cohort II (17 ReA and 21 upSpA), serum and SF cytokines were compared using a multiplex cytokine bead assay (27 cytokines). A total of 28 healthy controls with similar age and sex to cohort II were included for comparison of serum cytokine levels. Results In cohort I, HLA-B27 was positive in 81.8% (36/44) of ReA and 85.71% (48/56) of upSpA patients. HLA-B27 typing was successful in 70 patients (30 ReA and 40 uSpA). HLA-B*2705 was the most common, followed by HLA-B*2704 and HLA-B*2707. Frequencies were the same between ReA and upSpA. In cohort II, 14 cytokines were detectable in the serum of patients. The levels of eight cytokines were higher than in the controls. The cytokine levels of ReA and upSpA were similar. Sixteen cytokines were detectable in the SF of patients. There was no statistical difference in the levels between ReA and upSpA. The cytokine profiles in sera and SF were also similar among HLA-B27-positive and negative patients. Conclusion ReA and upSpA have similar HLA-B27 subtype associations and similar cytokine profiles. They should be considered as a single entity during studies as well as clinical management.

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