scholarly journals Luteolin Protects Pheochromocytoma (PC-12) Cells against Aβ25-35-Induced Cell Apoptosis through the ER/ERK/MAPK Signalling Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Han-Rui Wang ◽  
Si-Ying Pei ◽  
Dong-Xu Fan ◽  
Yan-Hui Liu ◽  
Xiao-Feng Pan ◽  
...  
Keyword(s):  
Cell Apoptosis ◽  
Positive Control ◽  
Signalling Pathway ◽  
Protective Effects ◽  
Pc 12 Cells ◽  
Mapk Signalling Pathway ◽  
Erk Mapk ◽  
Mapk Signalling ◽  
Luteolin Treatment

The regulatory effect of luteolin on the progression of Alzheimer’s disease (AD) remains unclear from the perspective of apoptosis. The present study aimed to investigate the protective effects of luteolin against Aβ25-35-induced cell apoptosis in pheochromocytoma (PC-12) cells. Aβ25-35 was used to induce an in vitro model of AD. Estradiol was used as a positive control. The PC-12 cells were incubated with luteolin alone or in combination with fulvestrant or U0126. The results showed that luteolin treatment significantly prevents Aβ25-35-induced decrease in cell viability and inhibits Aβ25-35-induced cell apoptosis. After the addition of fulvestrant and U0126, the apoptosis rate of PC-12 cells increased significantly. In addition, luteolin treatment significantly upregulated the expression of Bcl-2 and downregulated the expression of Bax and caspase-3, whereas fulvestrant and U0126 partially reversed the effects of luteolin. Moreover, luteolin treatment upregulated the expression of ERβ and p-ERK1/2, whereas fulvestrant blocked the expression of p-ERK1/2. The study showed that luteolin could activate the ER/ERK/MAPK signalling pathway to protect PC-12 cells against Aβ25-35-induced cell apoptosis via selectively acting on ERβ. Thus, luteolin may be considered as a potential novel therapeutic strategy for AD.

scholarly journals Anti-Angiogenic Efficacy of PSORI-CM02 and the Associated Mechanism in Psoriasis In Vitro and In Vivo

2021 ◽  
Vol 12 ◽  
Author(s):  
Yue Lu ◽  
Yuqi Yang ◽  
Junhong Zhang ◽  
Hongyu Zhang ◽  
Changju Ma ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Umbilical Vein ◽  
Oxidative Status ◽  
Signalling Pathway ◽  
Psoriatic Skin ◽  
Dependent Manner ◽  
Mapk Signalling Pathway ◽  
Mapk Signalling

Psoriasis is a chronic proliferative autoimmune dermatologic disease characterised by abnormal angiogenesis. Thus, regulating angiogenesis in the skin is an important treatment strategy for psoriasis. PSORI-CM02, an empirical Chinese medicine formula optimised from Yin Xie Ling, was created by the Chinese medicine specialist, Guo-Wei Xuan. Clinical studies have shown that PSORI-CM02 is safe and effective for the treatment of psoriasis. However, its anti-psoriatic mechanisms remain to be further explored. In this study, we investigated the effects of PSORI-CM02 on angiogenesis in the skin and the underlying mechanisms in IL-17A-stimulated human umbilical vein endothelial cells (HUVECs) and a murine model of imiquimod (IMQ)-induced psoriasis. In vitro, PSORI-CM02 significantly inhibited the proliferation and migration of IL-17A-stimulated HUVECs in a dose-dependent manner. Further, it markedly regulated the antioxidative/oxidative status and inflammation; suppressed the expression of VEGF, VEGFR1, VEGFR2, ANG1, and HIF-1α; and reduced the phosphorylation of MAPK signalling pathway components in IL-17A-stimulated HUVECs. In vivo studies showed that PSORI-CM02 markedly reduced angiogenesis in the skin of mice with IMQ-induced psoriasis, while significantly rebalancing antioxidant/oxidant levels; inhibiting the production of IL-6, TNF-α, IL-17A, and IL-17F; and repressing the synthesis of angiogenic mediators. In addition, PSORI-CM02 markedly reduced the activation of the MAPK signalling pathway in psoriatic skin tissue. Taken together, our results demonstrated that PSORI-CM02 inhibited psoriatic angiogenesis by reducing the oxidative status and inflammation, suppressing the expression of angiogenesis-related molecules, and inhibiting the activation of the MAPK signalling pathway in vitro and in vivo.

scholarly journals Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells

2020 ◽  
Vol 26 (7) ◽  
pp. 627-634 ◽  
Author(s):  
Zhang Zhu ◽  
Li Xueying ◽  
Li Chunlin ◽  
Xiong Wen ◽  
Zeng Rongrong ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Signalling Pathway ◽  
Inflammatory Factors ◽  
Mrna Levels ◽  
Protective Effects ◽  
Intestinal Epithelial ◽  
Mapk Signalling Pathway ◽  
Tnf Α ◽  
Mapk Signalling

Berberine is an alkaloid extracted from medicinal plants such as Coptis chinensis and Phellodendron chinense. It possesses anti-inflammatory, anti-tumour and anti-oxidation properties, and regulates Glc and lipid metabolism. This study explored the mechanisms of the protective effects of berberine on barrier function and inflammatory damage in porcine intestinal epithelial cells (IPEC-J2) induced by LPS. We first evaluated the effects of berberine and LPS on cell viability. IPEC-J2 cells were treated with 5 μg/ml LPS for 1 h to establish an inflammatory model, and 75, 150 and 250 μg/ml berberine were used in further experiments. The expression of IL-1β, IL-6 and TNF-α was measured by RT-PCR. The key proteins of the NF-κB/MAPK signalling pathway (IκBα, p-IκBα, p65, p-p65, c-Jun N-terminal kinase (JNK), p-JNK, p38, p-p38, ERK1/2 and p-ERK1/2) were detected by Western blot. Upon exposure to LPS, IL-1β, IL-6 and TNF-α mRNA levels and p-IκBα p-p65 protein levels were significantly enhanced. Pre-treatment with berberine reduced the expression of inflammatory factors and was positively correlated with its concentration, and dose dependently inhibited the expression of IκBα, p-IκBα, p-p65, p-p38 and JNK. These results demonstrated that pre-treating intestinal epithelial cells with berberine was useful in preventing and treating diarrhoea induced by Escherichia coli in weaned pigs.

scholarly journals ERK/MAPK signalling pathway and tumorigenesis (Review)

2020 ◽  
Author(s):  
Yan‑Jun Guo ◽  
Wei‑Wei Pan ◽  
Sheng‑Bing Liu ◽  
Zhong‑Fei Shen ◽  
Ying Xu ◽  
...  
Keyword(s):  
Signalling Pathway ◽  
Mapk Signalling Pathway ◽  
Erk Mapk ◽  
Mapk Signalling

scholarly journals Isoquercitrin inhibits the progression of liver cancer in vivo and in vitro via the MAPK signalling pathway

2014 ◽  
Vol 31 (5) ◽  
pp. 2377-2384 ◽  
Author(s):  
GUIHONG HUANG ◽  
BO TANG ◽  
KUN TANG ◽  
XIAOMIN DONG ◽  
JUNGANG DENG ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Signalling Pathway ◽  
Mapk Signalling Pathway ◽  
Mapk Signalling
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