Association of rs944289, rs965513, and rs1443434 in TITF1/TITF2 with Risks of Papillary Thyroid Carcinoma and with Nodular Goiter in Northern Chinese Han Populations

Objective. In this study, we aimed to investigate the associations of three single-nucleotide polymorphisms (SNPs) on TITF1/TITF2 (rs944289, rs965513, and rs1443434) with susceptibility to papillary thyroid carcinoma (PTC) and with nodular goiter (NG) in northern Chinese Han populations. Methods. We performed a case-control study comprising 861 PTC patients, 562 NG patients, and 896 normal controls (NCs). One TITF1 SNP (rs944289) and two TITF2 SNPs (rs965513 and rs1443434) were genotyped. Departures from Hardy–Weinberg equilibrium (HWE) in the control group were evaluated using chi-square test. Associations of the SNPs with PTC and with NG were assessed by unconditional logistic regression using the online SNPStats program. Bonferroni correction was performed for multiple tests in genotype analyses. Data analysis was performed by SPSS24.0 unless otherwise specified. Results. For rs944289, T allele was associated with increased risks for both PTC (OR = 1.23, 95% CI: 1.08–1.41, P=0.002) and NG (OR = 1.28, 95% CI: 1.10–1.50, P=0.002), and TT genotype significantly increased NG risk (recessive model, OR = 1.60, 95% CI: 1.22–2.10, P=0.001). For rs965513, no association was observed after Bonferroni correction. For rs1443434, G allele was associated with increased PTC risk (OR = 1.33, 95% CI: 1.10–1.61, P=0.003). Moreover, PTC risk increased with the number of total risk alleles of the three SNPs (OR = 1.25, 95% CI: 1.13–1.37, P<0.001). After stratified by gender, the risk effect of rs944289 T allele on PTC was only observed in females (OR = 1.29, 95% CI: 1.10–1.50, P=0.001). Individuals carrying rs944289-rs965513-rs1443434 haplotypes T-G-G and T-G-T had increased risks of PTC (OR = 1.82, 95% CI: 1.25–2.64, P=0.002) and NG (OR = 1.28, 95% CI: 1.06–1.54, P=0.011), respectively. Conclusions. There are associations of rs944289 and rs1443434 polymorphisms with PTC risk and association of rs944289 polymorphism with NG risk. Haplotypes T-G-G and T-G-T are risk haplotypes of PTC and NG, respectively.

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Altered TEG Parameters Identify Hypercoagulablilty and are of Diagnosis Value for Papillary Thyroid Carcinoma Patients

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/a-0723-3295 ◽

2018 ◽

Vol 128 (05) ◽

pp. 297-302

Author(s):

Songsong Lu ◽

Rui Kang ◽

Yongchao Wang ◽

Mengjie Zhu ◽

Lei Zhao ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Correlation Analysis ◽

Thyroid Carcinoma ◽

Roc Curve ◽

Nodular Goiter ◽

Roc Curves ◽

Control Group ◽

Healthy Controls ◽

Papillary Thyroid ◽

Routine Coagulation

Abstract Objective The goal of this study was to clarify the changes and clinical significance of thromboelastography (TEG) parameters in papillary thyroid carcinomas and nodular goiters. Methods 62 nodular goiter (NG) patients and 53 papillary thyroid carcinoma (PTC) patients were included. Coagulation indicators, together with a series of TEG parameters were collected and analyzed, compared with results of 61 healthy controls (HC). Correlation analysis was conducted between routine coagulation indicators and TEG parameters. ROC curves were used to evaluate the potential diagnosis value of the TEG parameters that were altered in papillary thyroid carcinoma patients. Results APTT and PT in papillary thyroid carcinoma group were statistically significant higher than that in control group (p<0.05). MPV was found to be higher in PTC than NG and healthy controls.R, K and SP levels were significantly lower in PTC group than that in HC group; Angle, CI and TPI levels were significantly higher in PTC group than that in HC group. Areas under the ROC curve of CI, TPI, and angle were 0.725, 0.714, and 0.687 for distinguishing PTC from HC, 0.662, 0.668 and 0.591 for distinguishing PTC from NG. Conclusion TEG parameters are altered and indicate hypercoagulablilty status of papillary thyroid carcinoma patients; CI, TPI, and angle could be potential diagnosis indicators for detecting papillary thyroid carcinoma.

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Higher urinary bisphenol A concentration and excessive iodine intake are associated with nodular goiter and papillary thyroid carcinoma

Bioscience Reports ◽

10.1042/bsr20170678 ◽

2017 ◽

Vol 37 (4) ◽

Cited By ~ 9

Author(s):

Zhenzhen Zhou ◽

Jing Zhang ◽

Fang Jiang ◽

Yan Xie ◽

Xiaochen Zhang ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Nodular Goiter ◽

Iodine Intake ◽

Control Group ◽

Papillary Thyroid ◽

Excessive Iodine ◽

Gender Specific Differences ◽

Gender Specific ◽

Excessive Iodine Intake

In the present study, we investigated whether bisphenol A (BPA) levels and excessive iodine intake were associated with papillary thyroid carcinoma (PTC) and nodular goiter (NG). We determined total BPA concentrations (TBC) in paired serum and urine samples, and urinary iodine concentrations (UIC) in urine samples collected from PTC patients, NG patients, and healthy individuals, then compared BPA concentrations and UIC within and between each patient group. The results showed that there were no gender-specific differences in serum TBC and UIC in each group, and no differences across all patient groups. Urinary BPA concentrations (UBC) were higher in the NG and PTC groups compared with the control group. UBC showed gender-specific differences in the NG and PTC group. Furthermore, UIC were higher in the NG and PTC groups compared with the control group. Higher UBC and excessive iodine intake were risk factors for NG and PTC according to multivariate logistic regression analysis. There was a significant correlation between UBC and UIC in each group. These data suggested that higher UBC and excessive iodine intake are associated with NG and PTC. The metabolic and functional pathways between BPA and iodine are potentially linked to the pathogenesis and progression of NG and PTC.

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Assessment Of Plasma B-Catenin Concentration As Biomarker Of Thyroid Cancer*

Polish Journal of Surgery ◽

10.1515/pjs-2015-0067 ◽

2015 ◽

Vol 87 (7) ◽

Cited By ~ 1

Author(s):

Michał Bełdowski

Keyword(s):

Thyroid Cancer ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Histopathological Examination ◽

Nodular Goiter ◽

Diagnostic Methods ◽

Control Group ◽

Papillary Thyroid ◽

It Use ◽

Cellular Expression

AbstractNew diagnostic methods for thyroid diseases are still being searched for. Immunohistochemical diagnosis is expanded by the introduction of new biomarkers including ß-catenin (B-Cat). Associations are indicated between the cellular expression of this biomarker and tumor stage, nodal metastases and the degree of tumor cell differentiation. Reports are scarce regarding the plasma level of this biomarker in malignant neoplastic diseases.was to analyze the plasma B-Cat concentration and the possibility of it use in the diagnostics of patients with nodular goiter and papillary thyroid carcinoma.Plasma B-Cat concentration was determined in 64 patients with goiter and 15 healthy volunteers. The final histopathological examination revealed 41 cases of papillary thyroid carcinoma (PTC) and 13 cases of nodular goiter (NG).A significant increase in B-Cat (p <0.05) in both groups compared to the control group. No differences in the concentrations of biomarker was demonstrated between the PTC and NG groups. After determining the AUC for the tested biomarker, the B-Cat ratio of the area value 0.721 was the strong diagnostic test.Changes in the plasma B-Cat concentration can be the biomarker of thyroid cancer but it cannot be used for the detection of papillary thyroid carcinoma becouse of concomitant tumor-like lesions in the thyroid gland.

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Multiple Endocrine Neoplasia Type 1 Combined With Papillary Thyroid Carcinoma and Nodular Goiter: A Case Report and Review of The Literature

10.21203/rs.3.rs-92002/v1 ◽

2020 ◽

Author(s):

Jialu Xu ◽

Su Dong ◽

Zhe Han ◽

Lele Sun ◽

Jia Liu

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Multiple Endocrine Neoplasia ◽

Multiple Endocrine Neoplasia Type ◽

Nodular Goiter ◽

Surgical Removal ◽

Papillary Thyroid ◽

Endocrine Neoplasia ◽

Endocrine Neoplasia Type

Abstract Introduction: This is the first report of multiple endocrine neoplasia type 1 (MEN1) combined with papillary thyroid carcinoma (PTC) and nodular goiter in China. MEN1 is a rare hereditary tumor syndrome inherited in an autosomal dominant manner and presenting mostly as tumors of the parathyroid, endocrine pancreas (such as gastrinoma) and anterior pituitary. However, PTC and nodular goiter were not previously regarded as components.Patient concerns: We present a 35-year-old woman with MEN1 accompanied with coinstantaneous PTC and nodular goiter.Diagnosis: The pathological diagnosis was PTC with cervical lymph node metastasis, nodular goiter, parathyroid cyst and adenomatoid hyperplasia. Genetic analysis was performed and a germ-line MEN1 gene mutation was detected.Interventions: The patient underwent unilateral lobectomy of the thyroid gland and surgical removal of the parathyroid tumors.Outcomes: After 6 months of follow-up, ultrasonic examination of the patient’s neck showed no abnormality. Serum calcium and parathyroid hormone levels were normal.Conclusion: This is the first experience of a case of MEN1 combined with PTC and nodular goiter in China. MEN1 syndrome may have predisposed the present patient to PTC and nodular goiter. PTC and/or nodular goiter may be new components of MEN1. More cases are required to confirm this association.

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Abstract 2215: Association between single-nucleotide polymorphisms of BRAF and papillary thyroid carcinoma in a Chinese population

10.1158/1538-7445.am2014-2215 ◽

2014 ◽

Author(s):

Kexin Chen

Keyword(s):

Papillary Thyroid Carcinoma ◽

Single Nucleotide Polymorphisms ◽

Thyroid Carcinoma ◽

Chinese Population ◽

Nucleotide Polymorphisms ◽

Papillary Thyroid ◽

Single Nucleotide

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Association between the KRAS Gene Polymorphisms and Papillary Thyroid Carcinoma in a Chinese Han Population

Journal of Cancer ◽

10.7150/jca.16507 ◽

2016 ◽

Vol 7 (15) ◽

pp. 2420-2426 ◽

Cited By ~ 4

Author(s):

Lifeng Ning ◽

Wenwang Rao ◽

Yaqin Yu ◽

Xiaoli Liu ◽

Yuchen Pan ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Gene Polymorphisms ◽

Chinese Han Population ◽

Papillary Thyroid ◽

Chinese Han ◽

Kras Gene ◽

Han Population

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A meta-analysis of circulating microRNAs in the diagnosis of papillary thyroid carcinoma

PLoS ONE ◽

10.1371/journal.pone.0251676 ◽

2021 ◽

Vol 16 (5) ◽

pp. e0251676

Author(s):

Yan liu ◽

Houfa Geng ◽

Xuekui Liu ◽

Mingfeng Cao ◽

Xinhuan Zhang

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Likelihood Ratio ◽

Meta Analysis ◽

Positive Likelihood Ratio ◽

Negative Likelihood Ratio ◽

Diagnostic Value ◽

Control Group ◽

Papillary Thyroid ◽

Circulating Micrornas

Background Aim of this meta-analysis was to evaluate the overall diagnostic value of circulating mini miRNAs for papillary thyroid carcinoma (PTC) and to find the possible molecular marker with higher diagnostic value for PTC. Methods We searched the Pubmed, Cochrane and Embase database until June 2020. We selected relevant literatures associated with the diagnosis of PTC with circulating miRNAs. The number of cases in experimental group and the control group, sensitivity and specificity could be extracted from the literatures. Results We got 9 literatures including 2114 cases of PTC. Comprehensive sensitivity was 0.79, comprehensive specificity was 0.82, positive likelihood ratio was 4.3, negative likelihood ratio was 0.26, diagnostic advantage ratio was 16. The summary receiver operating characteristic curve was drawn and the Area Under the Curve was 0.87. Conclusions Circulating microRNAs may be promising molecular markers for the diagnosis of papillary thyroid carcinoma. Combined detection of certain serum microRNAs can improve the diagnostic accuracy of papillary thyroid carcinoma. Especially MiR-222 and miR-146b may be prime candidates for the diagnosis of PTC in Asian population.

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Genetic predisposition to papillary thyroid carcinoma is mediated by a long non-coding RNA TINCR enhancer polymorphism

10.21203/rs.3.rs-1023538/v1 ◽

2021 ◽

Author(s):

Qiang Wang ◽

Hong Huang ◽

Peng Chen ◽

Xiao Xiao ◽

Xiaolei Luo ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Cancer Susceptibility ◽

Luciferase Reporter ◽

Nucleotide Polymorphisms ◽

Papillary Thyroid ◽

Enhancer Activity ◽

Enhancer Region ◽

Non Coding Rna ◽

The Impact

Abstract Single nucleotide polymorphisms (SNPs) in the enhancer region have been demonstrated to confer to altered enhancer activities, aberrant gene expression, and cancer susceptibility. In this study, we aimed to examine the association between an SNP, rs8101923, within terminal differentiation-induced non-coding RNA (TINCR) and the risk of papillary thyroid carcinoma (PTC). Blood samples from 559 patients with PTC and 445 healthy individuals were collected. The rs8101923 was genotyped by using polymerase chain reaction-restriction fragment length polymorphism assay. The impact of the rs8101923 on TINCR expression and enhancer activity was evaluated by quantitative real-time PCR and dual-luciferase reporter assay. The binding of AP-2α to TINCR enhancer was determined by chromatin immunoprecipitation. The rs8101923 G allele was significantly associated with a higher risk of PTC (adjusted OR = 1.37; 95% CI: 1.15–1.64). Mechanistically, the rs8101923 was related to increased transcriptional levels and enhancer activities (P < 0.05). Transcription factor AP-2α binds to the enhancer region of TINCR containing the rs8101923 locus, and promotes cell proliferation in PTC. These findings suggest the rs8101923 as a risk factor in the pathogenesis of PTC, which provides evidence for explaining the mechanism of the rs8101923 risk allele predisposing to PTC.

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Gold Nanoparticles Suppressed Proliferation, Migration, and Invasion in Papillary Thyroid Carcinoma Cells via Downregulation of CCT3

Journal of Nanomaterials ◽

10.1155/2019/1687340 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Fangzhou Liu ◽

Dawei Ma ◽

Wei Chen ◽

Xinyuan Chen ◽

Yichun Qian ◽

...

Keyword(s):

Gold Nanoparticles ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Molecular Mechanisms ◽

Target Therapy ◽

Migration And Invasion ◽

Control Group ◽

Papillary Thyroid ◽

And Migration ◽

Induced Apoptosis

Emerging evidences have demonstrated that gold nanoparticles (AuNPs) have been used for cancer treatment. The aim of this study was to investigate the effects and molecular mechanisms of AuNPs on papillary thyroid carcinoma (PTC) cells (BCPAP and TPC-1). Characterizations of AuNPs were detected by UV-Vis spectra, transmission electron microscopy (TEM), and dynamic light scattering (DLS). Cell proliferation and apoptosis, migration, and invasion of PTC cells were evaluated by MTT, flow cytometry, wound healing, and transwell assays, respectively. Furthermore, qRT-PCR and western blot assays were performed to assess the protein expressions related to apoptosis and migration including caspase-3, caspase-9, Bax, Bcl-2, MMP-2, and MMP-9. The study revealed that AuNPs significantly suppressed cell viability, migration, and invasion and remarkably induced apoptosis of BCPAP and TPC-1 cells compared with the control group. Moreover, AuNPs negatively regulated the expression of CCT3 and silencing of CCT3 obviously promoted the proliferation, migration, and invasion inhibition and apoptosis induction of PTC cells combined with AuNPs. Collectively, these results highlighted the potential application of AuNPs in PTC target therapy.

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