scholarly journals Retrospective Analysis of 2019-nCov-Infected Cases in Dongyang, Southeastern China

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
G. G. Li ◽  
Z. Lv ◽  
Y. S. Wang ◽  
J. F. Li ◽  
L. F. Feng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Clinical Samples ◽  
Test Results ◽  
Infected People ◽  
Sporadic Cases ◽  
Asymptomatic Individuals ◽  
Novel Coronavirus ◽  
Acid Test ◽  
Negative Conversion

The 2019 novel coronavirus (2019-nCov) has caused increasing number of infected cases globally. This study was performed to analyze information regarding the transmission route and presence of viral nucleic acids on several clinical samples. Confirmed 2019-nCov-infected cases were identified in Dongyang and were treated according to guidelines for the diagnosis of 2019-nCov infection released by the National Health Commission. Information regarding the contacts that the infected people had was collected to determine whether it caused clustered cases. A series of successive nucleic acid examination of feces, oropharyngeal swabs, and sputum was also performed, and the results were analyzed. A total of 19 confirmed cases of 2019-nCov infection were identified in Dongyang, Zhejiang Province, China. Five cases showed severe symptoms, and the remaining ones showed mild manifestations. Ten cases infected from two asymptomatic individuals were clustered into two groups. Among 14 cases with consecutive nucleic acid test results, four patients showed positive results in feces after their negative conversion in oropharyngeal swabs. Asymptomatic individuals with the virus could cause 2019-nCov clustered cases, and the clustered cases may differ from sporadic cases on age and length of hospitalization. In addition, nucleic acids in feces last longer than those in oropharyngeal swabs.

scholarly journals Cases Report of Cured Novel Coronavirus-infected Pneumonia Patients with Viral Nucleic Acid Test Positive in Fecal Specimens

2020 ◽  
Author(s):  
Mei Han ◽  
Jingbo Zou ◽  
Wenguang Tian ◽  
Xiaoyu Wei ◽  
Yang Zhou ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnostic ◽  
Clinical Practices ◽  
Rt Pcr ◽  
Viral Nucleic Acid ◽  
Nucleic Acid Test ◽  
Assessment Standard ◽  
Novel Coronavirus ◽  
Acid Test ◽  
Respiratory Specimens

Abstract Background: The outbreak of the novel coronavirus in China (COVID-19) represents a significant and urgent threat to global health. We report here five cases of COVID-19 infection patients in our clinical practices who are medically stable and presumed to successfully “cleared” the virus after antiviral treatments. Case presentation: The clinical evaluation depends on the viral nucleic acid test in respiratory specimens by real-time PCR reverse transcription (RT-PCR) assays according to the authorized guidance. We found that the stool samples of these cured patients remain positive in RT-PCR assay while the virus is undetectable in respiratory specimens. RT-PCR molecular diagnostic assay was designed to specifically detect the presence of viral RNA. Thus, the positive result in the fecal specimens implies the existence of viable virions with the patients. Conclusions: This highlights the importance to look closely at the assessment standard of medical treatment, as well as the need for reevaluation of the criteria for the initial screening, prevention, and care of patients with this emerging infection.

Prevalence and risk factors associated with repeat positive SARS-CoV-2 nucleic acid test results among discharged COVID-19 patients

Medicine ◽  
2021 ◽  
Vol 100 (48) ◽  
pp. e27933
Author(s):  
Yanru Cui ◽  
Jilin Wang ◽  
Gaofeng Wang ◽  
Xiuguo Xie ◽  
Lizhen Tian
Keyword(s):  
Risk Factors ◽  
Nucleic Acid ◽  
Test Results ◽  
Nucleic Acid Test ◽  
Factors Associated ◽  
Acid Test

scholarly journals COVID-19 Pneumonia: A Review of Typical Ct Findings and Differential Diagnosis

2020 ◽  
Vol 1 (3) ◽  
pp. 01-22
Author(s):  
Anthony Venyo
Keyword(s):  
Ct Scan ◽  
Imaging Techniques ◽  
Systemic Disease ◽  
Variable Number ◽  
Emergency Setting ◽  
Test Results ◽  
Imaging Characteristics ◽  
Novel Coronavirus ◽  
High Index Of Suspicion ◽  
Acid Test

Pneumonia that is caused by the 2019 novel coronavirus (SARS-CoV-2, which is also referred to as 2019-nCoV recently did break out in Wuhan China has been coined the terminology of COVID-19. With the spread of the disease, similar cases of COVID-19 had been confirmed in various regions of the world. Because COVID-19 is a relatively new global disease, clinicians, and patients across the globe would initially not be conversant with the clinical features and radiology imaging characteristics of SARS-CoV-2 pneumonia. The causes of pneumonia are protein, many secondary to an underlying cardiorespiratory abnormality while some are related systemic disease. Various imaging techniques generally diagnose cases of Pneumonia. In the current climate, COVID-19 Pneumonia has taken center stage; confirmation relies upon microbiological studies such as real-time polymerase chain reaction or sequencing. These investigations are not usually available in an emergency setting. Computed Tomography (CT) can be used as an essential complement for the diagnosis of COVID-19 Pneumonia in the current epidemic context. But the later may be misleading as other cases of Pneumonia, and interstitial lung disease can easily be confused with COVID-19 Pneumonia. Also, Covid19 Pneumonia may be missed if not considered. The attention of clinicians should be alerted to the possibility of COVID-19 to conduct the appropriate tests to confirm or negate the diagnosis of COVID-19. In asymptomatic as well as in symptomatic patients that have COVID1-9 pneumonia, the initial COVID-19 nuclei acid test results could be normal, which upon subsequent repeat testing would become normal. Still, radiology imaging using a CT scan of thorax would tend to demonstrate various non-specific features that affect a variable number of lobes of the lungs, and these features quickly increase in size when a repeat CT scan of the thorax is undertaken. These findings tend to predate positive COVID-19 test results in some cases of COVID-19. The non-specific changes tend to resolve when the patient resolves from COVID-19 pneumonia. A catalog of radiology images that demonstrate various types of cardio-pulmonary lesions which when encountered by clinicians should alert them to exclude the possibility of COVID-19 Pneumonia has been included in the paper as an aid to alerting clinicians to have a high index of suspicion of radiology images of the thorax which should help them to quickly undertake appropriate tests to confirm or negate the diagnosis of COVID-19 pulmonary infection.

scholarly journals Persistent positivity of SARS-CoV-2 nucleic acid in asymptomatic healthcare worker: infective virion or inactive nucleic acid?

2021 ◽  
Vol 14 (3) ◽  
pp. e241087
Author(s):  
Lokesh Tiwari ◽  
Prakriti Gupta ◽  
Chandra Mani Singh ◽  
Prabhat Kumar Singh
Keyword(s):  
Nucleic Acid ◽  
Healthcare Worker ◽  
Significant Proportion ◽  
Test Duration ◽  
Upper Respiratory Tract ◽  
Occupational Hazard ◽  
Upper Respiratory ◽  
Asymptomatic Individuals ◽  
Acid Test

Asymptomatic individuals positive for SARS-CoV-2 RNA constitute a significant proportion of the infected population and play a role in the transmission of the virus. We describe a healthcare worker who presented with fever and malaise and was diagnosed with mild COVID-19. The symptoms resolved within 4 days but there was persistent positivity of viral RNA in the upper respiratory tract for more than 58 days, which is the longest reported duration of persistence of SARS-CoV-2 in a healthcare worker. In this case report, we discuss clinical and administrative issues such as the role of asymptomatic cases in the transmission of the virus to patients and coworkers as an occupational hazard, interpretation of persistent positivity of nucleic acid test, duration of isolation and return-to-work guidelines pertinent to researchers and global health policymakers.

scholarly journals ClinicalReportsonEarlyDiagnosisofNovelCoronavirus （2019-nCoV）PneumoniainStealthInfectedPatients

2020 ◽  
Author(s):  
Le Yang
Keyword(s):  
Nucleic Acid ◽  
Early Diagnosis ◽  
Early Identification ◽  
Clinical Evidence ◽  
Critical Role ◽  
Clinical Manifestations ◽  
Clinical Analysis ◽  
Chest Ct ◽  
Novel Coronavirus ◽  
Acid Test

An outbreak of novel coronavirus(2019-nCoV) that began in Wuhan, China, is rapidly spreading to all over China, and gradually to multiple countries. Regarding to put the epidemic prevention and spread under the control, early identification and diagnosis play a critical role. Patients with initially no or mild symptoms of Novel Coronavirus Pneumonia (2019-nCoV), namely stealth infection, often lack of typical clinical evidence to establish the diagnosis. Based on the clinical analysis of 4 cases in stealth infection, the results of this study highlight that early diagnosis requires a combination of epidemiological history, clinical manifestations, early chest CT examination, and 2019-nCoV RNA test (nucleic acid test), with particular emphasis on definite epidemiological history and early chest CT findings when positivenucleicacidtestslagbehind.

scholarly journals Urine nucleic acid test positive in an asymptomatic patient with novel coronavirus 2019 infection: a case report

2020 ◽  
Author(s):  
Yaoyong Wang ◽  
Xinjian Chang ◽  
Jianguo Ren ◽  
Dongyan Li ◽  
Changfei Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Throat Swab ◽  
Asymptomatic Patient ◽  
Clinical Manifestations ◽  
Nucleic Acid Test ◽  
Asymptomatic Patients ◽  
Sources Of Infection ◽  
Novel Coronavirus ◽  
Acid Test

Abstract Background: With the emergence of coronavirus disease 2019 in many places around the world, the main medical resources currently focus on the treatment of confirmed patients and the screening of suspected cases. Asymptomatic patients are difficult to detect, but they may be contagious, which makes epidemic control more difficult. We found a case of asymptomatic patient with positive urine coronavirus nucleic acid test, and we hope to attract attention of all circles.Case presentation: An asymptomatic patient with novel coronavirus infection was found in an epidemiological investigation of patients with confirmed coronavirus disease 2019. The patient was admitted to the hospital on February 24, 2020. She had no clinical manifestations such as fever, dry cough, and fatigue, and no abnormal signs. The examination showed that her throat swab was negative for nucleic acid but the urine was positive for nucleic acid. She was given antiviral and symptomatic supportive treatment. On February 26, her throat swab was checked for nucleic acid positive. On March 3 and 5, her throat swab and urine nucleic acid were negative. On March 9 and 12, her throat swab nucleic acid were negative. The patient was discharged from the hospital on March 13 and continued to be isolated and observed outside the hospital. Follow-up was conducted on March 26, the patient did not have any discomfort, the throat swab nucleic acid test was negative, and the isolation was lifted.Conclusion: There are asymptomatic patients with coronavirus disease 2019, and their urine may be one of the sources of infection.

scholarly journals Development of Recombinase Aided Amplification Combined With Disposable Nucleic Acid Test Strip for Rapid Detection of Porcine Circovirus Type 2

2021 ◽  
Vol 8 ◽  
Author(s):  
Wenxian Chen ◽  
Jindai Fan ◽  
Zhaoyao Li ◽  
Yuanyuan Zhang ◽  
Yuwei Qin ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Test Strip ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Clinical Samples ◽  
Gene Fragment ◽  
Nucleic Acid Test ◽  
Orf2 Gene ◽  
Acid Test

Porcine circovirus type 2 (PCV2) is the dominant causative agent of PCV2 systemic disease (PCV2-SD) in pigs. It can also associate with other diseases such as respiratory and enteric diseases, reproductive failure, porcine dermatitis and nephropathy syndrome in pigs. Currently, PCV2 infection is a considerable threat in the swine industry. Therefore, it is of great significance to prevent, control, and accurately detect PCV2 in pig farms. Recombinase aided amplification (RAA) technology is an isothermal nucleic acid amplification technology that could rapidly amplify the target gene fragment at a constant temperature. The amplification products labeled with specific molecules could be visually detected using the test strip with the corresponding antibody. In the present study, the RAA technology combined with a nucleic acid test strip (RAA-strip) was established for simple and specific detection of PCV2. Primers and probes targeting the PCV2 ORF2 gene were designed according to the RAA technology principles. The PCV2 RAA-strip established in this study could detect as low as 103 copies/μL of recombinant plasmids containing the PCV2 ORF2 gene fragment. The lowest detection limit about viral DNA and virus titers was 6.7 × 10−6 ng/μL and 10 TCID50/mL, respectively. Furthermore, no cross-reaction with other porcine viruses occurred at 37°C and within 15 min. We used 42 clinical samples to assess the performance of our established method. The positive rate of clinical samples detected by PCV2 RAA-strip was 50.00%. This was similar to that detected by PCV2 PCR (45.24%). In conclusion, due to the advantages of strong specificity, high sensitivity, excellent reproducibility, and simple operation method, our PCV2 RAA-strip is suitable for the rapid clinical detection of PCV2 on-site.

scholarly journals COVID-19 versus non-COVID-19 pneumonia: A retrospective cohort study in Chengdu, China

2020 ◽  
Author(s):  
Xiao-Jin Li ◽  
Bing-Xing Shuai ◽  
Zhong-Wei Zhang ◽  
Yan Kang
Keyword(s):  
Nucleic Acid ◽  
Retrospective Cohort ◽  
Clinical Characteristics ◽  
Epidemiological Data ◽  
Group Differences ◽  
Test Results ◽  
Imaging Findings ◽  
Viral Nucleic Acid ◽  
Nucleic Acid Test ◽  
Acid Test

Background and ObjectiveSince the outbreak of coronavirus disease 2019 (COVID-19) in December 2019 in Wuhan, Hubei Province, China, it has spread throughout the world and become a global public health emergency. It is important to distinguish COVID-19 from other viral pneumonias to properly screen and diagnose patients, reduce nosocomial infections, and complement the inadequacy of nucleic acid testing. In this study, we retrospectively analysed the clinical data of COVID-19 versus non-COVID-19 patients treated at our hospital between January 17 and February 27, 2020 to summarize our clinical experience in the differential diagnosis of COVID-19.MethodsIn this retrospective cohort study, 23 confirmed COVID-19 patients were consecutively enrolled from January 17 to February 27, 2020, and 29 confirmed non-COVID-19 patients were enrolled in the West China Hospital of Sichuan University. We collected baseline data, epidemiological data, clinical characteristics, imaging findings, viral nucleic acid test results, and survival data. SPSS v22.0 was used for the statistical analysis. Outcomes were followed-up until March 25.ResultsA total of 52 patients were included in this study, including 23 COVID-19 patients and 29 non-COVID-19 patients. No significant between-group differences were observed for age, sex, primary signs or symptoms, cellular immunity, or platelet count. Significant between-group differences were observed in clinical characteristics such as dry cough, contact with individuals from Wuhan, some underlying diseases, nucleated cell count, chest imaging findings, viral nucleic acid test results, 28-day mortality, and 28-day survival.ConclusionEpidemiological data, clinical symptoms, nucleic acid test results for COVID-19 and chest CT manifestation may help distinguish COVID-19 from non-COVID-19 cases, prevent imported cases and nosocomial infections.

scholarly journals A Research on the Results of Viral Nucleic Acid Tests and CT Imaging Variation of Patients with COVID-19

2020 ◽  
Author(s):  
Meng Xu ◽  
Xun Liu ◽  
Chuhong Su ◽  
Yuping Zeng ◽  
Jinqian Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Recovery Period ◽  
Ct Imaging ◽  
Nucleic Acid Detection ◽  
Test Results ◽  
Viral Nucleic Acid ◽  
Nucleic Acid Test ◽  
Acid Test ◽  
Pulmonary Ct ◽  
Nucleic Acid Tests

AbstractBackgroundCoronavirus disease 2019 (COVID-19) has become a global health problem. We aim to investigate the changes in the results of viral nucleic acid tests on pharyngeal swabs and feces of patients with COVID-19 and CT imaging of lungs as the disease progresses.MethodsSeven patients with COVID-19 in the third affiliated hospital of Sun Yat-sen University Yuedong Hospital were retrospectively enrolled with clinical features, including imaging staging, and performance characteristics of viral nucleic acid test results of pharyngeal swabs and feces. The dynamic changes of these features were observed during hospitalization, and therapeutic effect and prognosis of patients were evaluated.ResultsThe results of seven cases with COVID-19 were positive for viral nucleic acid tests on pharyngeal swabs early after the onset of symptoms, and then turned negative; while the results of viral nucleic acid tests on feces were persistently positive in the mid-term clinical treatment and recovery period. And the viral nucleic acid test results were capricious in three cases. Pulmonary CT imaging showed characteristic changes in early, advanced and recovery phases.ConclusionThe application of viral nucleic acid detection and pulmonary CT imaging can be used for screening of suspected cases. Fecal nucleic acid test should be recommended as the reference of discharge standard, in order to minimize the risk of transmission from digestive tract.

Sign in / Sign up

Export Citation Format

Share Document