scholarly journals Quantification Analysis of 13 Organic Components and 8 Inorganic Elements in Angelica Sinensis Radix and Its Different Parts Combined with Chemical Recognition Pattern

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Xi Li ◽  
Yixin Yao ◽  
Xiaoxiao Wang ◽  
Chang An ◽  
Shanshan Gao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Phenolic Acids ◽  
Inductively Coupled Plasma ◽  
Angelica Sinensis ◽  
Inorganic Elements ◽  
Organic Components ◽  
Chemical Recognition ◽  
Significant Difference ◽  
Angelica Sinensis Radix ◽  
Different Parts

Angelica Sinensis Radix (Danggui, DG) is one of the most commonly prescribed traditional Chinese medicines. The organic components include phthalides and phenolic acids. Meanwhile, inorganic elements play an important role in clinical effect. DG and its different parts have different effects. There is no relevant report on the analysis of organic compounds and inorganic elements among them. Therefore, ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry was developed for the simultaneous determination of 13 organic components (8 phthalides and 5 phenolic acids), and 8 inorganic elements were determined by inductively coupled plasma mass spectrometry. The contents of 32 samples were analyzed by orthogonal partial least squares discrimination analysis, hierarchical cluster analysis, and least-significant difference of one-way analysis of variance. The results showed that the differences were significant among DG and its different parts. 11 difference markers (Ca, Z-ligustilide, Mg, Mn, Fe, Na, K, Cu, Zn, coniferyl ferulate, and senkyunolide A) were obtained by variable importance for the project. These difference markers were some different among DG and its different parts, especially Z‐ligustilide, coniferyl ferulate, Mg, Zn, the differences were significant. This study can provide a reference for DG research.

Two Novel Thio-Arsenosugars in Scallops Identified with HPLC - ICPMS and HPLC - ESMS

2005 ◽  
Vol 2 (3) ◽  
pp. 171 ◽  
Author(s):  
Markus Kahn ◽  
Reingard Raml ◽  
Ernst Schmeisser ◽  
Birgit Vallant ◽  
Kevin A. Francesconi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
High Performance ◽  
Dry Mass ◽  
New Method ◽  
Ionization Mass ◽  
Arsenic Compounds ◽  
Freeze Dried ◽  
Significant Difference ◽  
Seafood Products

Environmental Context. A new group of arsenic compounds, namely thio-arsenosugars, has recently been discovered in some marine samples, including seafood products. We do not yet understand how these arsenic compounds are produced by living organisms, nor do we know their implications for human health. Their unusual chromatographic properties are the reason that they are difficult to detect by existing methods. We present a new method for finding these thio-arsenosugars, and we also report two new thio-arsenosugars. Abstract. Two new thio-arsenosugars were identified in aqueous and methanol extracts from the gonad and the muscle of the great scallop by using high-performance liquid chromatography–inductively coupled plasma mass spectrometry (HPLC–ICPMS) and HPLC–electrospray ionization mass spectrometry (ESMS). The gonad of the scallops contained thio-arsenosugar–sulfonate [0.067 mg (As) kg−1; dry mass ± 3.7%] and thio-arsenosugar–sulfate [0.267 mg (As) kg−1; dry mass ± 1.2%]. The muscle also contained both thio-arsenosugar–sulfonate [0.030 mg (As) kg−1; dry mass ± 2.6%] and thio-arsenosugar–sulfate [0.200 mg (As) kg−1; dry mass ± 2.6%]. Methanol increased the extraction efficiency threefold for the two thio-arsenosugars as compared to water. Fresh and freeze-dried samples from both parts of the scallop showed no significant difference with respect to the concentration of the two thio-arsenosugars. In order to confirm the presence of the thio-arsenosugars, a new method for their determination with HPLC–ESMS was developed. Under optimized conditions [Hamilton PRP-X100; 150 by 1.0 mm; 20 mM NH4HCO3 with 55% (v/v) methanol at pH 10.3] we succeeded in determining the pseudomolecular ions of thio-arsenosugar–glycerol, thio-arsenosugar–phosphate, thio-arsenosugar–sulfonate, and thio-arsenosugar–sulfate at concentrations less than 5 µg (As) dm−3 in standard solutions and real samples.

scholarly journals Analysis of Sr and Ba profiles measured by ultra-high-resolution mass spectrometry LA-ICP-MS in otoliths of juvenile anadromous sockeye salmon Oncorhynchus nerka in the early marine life-history stage as a proxy for fresh to marine water transition

2021 ◽  
Vol 201 (3) ◽  
pp. 669-685
Author(s):  
Yu. Egorova ◽  
Yu. Kuzmenko ◽  
T. Spesivy ◽  
B. P.V. Hunt ◽  
E. A. Pakhomov
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Sockeye Salmon ◽  
High Resolution Mass Spectrometry ◽  
Oncorhynchus Nerka ◽  
Life History Stage ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Reliable Marker

The strontium (Sr) and barium (Ba) profiles in otoliths of juvenile sockeye salmon Oncorhynchus nerka from British Columbia are measured using a Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) system and analyzed. The highest possible measurement resolution (near-daily) was used to assess variability and repeatability of the breakpoint (marine entry) estimates inferred from Sr:Ca and Ba:Ca ratios. Such resolution for the otolith chemical composition (to an accuracy of 2 μm) was reached using the rotating slit, which width was close to the daily circulus width of the otoliths. So, daily or 2-day changes in the elemental composition were recorded during the period of transition to the marine environment. Sr profiles were generally similar among the fish, starting with low values of Sr:Ca in the fresh water and increasing sharply after the marine entry. The Ba:Ca signal was more complex, showing in most cases a dramatic increase immediately before the breakpoint. Besides, multiple peaks in the Ba profiles were recorded prior to the marine transition with a significant difference of their number between fish from different populations. A breakpoint was detectable in the Ba profiles 3–11 µm prior to its appearance in the Sr profiles. The complexity of Ba profiles may cause erroneous estimates of the marine entry date; thus, the Sr signal is a more reliable marker of marine transition for juvenile sockeye. 

scholarly journals Mapping the Inorganic and Proteomic Differences among Different Types of Human Teeth: A Preliminary Compositional Insight

Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1540
Author(s):  
Vaibhav Sharma ◽  
Simran Rastogi ◽  
Kaushal Kumar Bhati ◽  
Alagiri Srinivasan ◽  
Ajoy Roychoudhury ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Hierarchical Structures ◽  
Inorganic Materials ◽  
Human Teeth ◽  
Inductively Coupled ◽  
Protein Protein Interaction ◽  
Organic Components ◽  
Inorganic And Organic

In recent years, studies on mineralized tissues are becoming increasingly popular not only due to the diverse mechanophysical properties of such materials but also because of the growing need to understand the intricate mechanism involved in their assembly and formation. The biochemical mechanism that results in the formation of such hierarchical structures through a well-coordinated accumulation of inorganic and organic components is termed biomineralization. Some prime examples of such tissues in the human body are teeth and bones. Our current study is an attempt to dissect the compositional details of the inorganic and organic components in four major types of human teeth using mass spectrometry-based approaches. We quantified inorganic materials using inductively coupled plasma resonance mass spectrometry (ICP-MS). Differential level of ten different elements, Iron (Fe), Cadmium (Cd), Potassium (K), Sulphur (S), Cobalt (Co), Magnesium (Mg), Manganese (Mn), Zinc (Zn), Aluminum (Al), and Copper (Cu) were quantified across different teeth types. The qualitative and quantitative details of their respective proteomic milieu revealed compositional differences. We found 152 proteins in total tooth protein extract. Differential abundance of proteins in different teeth types were also noted. Further, we were able to find out some significant protein-protein interaction (PPI) backbone through the STRING database. Since this is the first study analyzing the differential details of inorganic and organic counterparts within teeth, this report will pave new directions to the compositional understanding and development of novel in-vitro repair strategies for such biological materials.

scholarly journals Evaluation of an Element-Tagged Duplex Immunoassay Coupled with Inductively Coupled Plasma Mass Spectrometry Detection: A Further Study for the Application of the New Assay in Clinical Laboratory

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5370 ◽  
Author(s):  
Wencan Jiang ◽  
Gongwei Sun ◽  
Wenbin Cui ◽  
Shasha Men ◽  
Miao Jing ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Clinical Laboratory ◽  
Clinical Sample ◽  
Measurement Range ◽  
Clinical Samples ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry

Background: Element-tagged immunoassay coupled with inductively coupled plasma mass spectrometry (ICP-MS) detection has the potential to revolutionize immunoassay analysis for multiplex detection. However, a further study referring to the standard evaluation and clinical sample verification is needed to ensure its reliability for simultaneous analysis in clinical laboratories. Methods: Carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) were chosen for the duplex immunoassay. The performance of the assay was evaluated according to guidelines from the Clinical and Laboratory Standards Institute (CLSI). Moreover, reference intervals (RIs) of CEA and AFP were established. At last, 329 clinical samples were analyzed by the proposed method and results were compared with those obtained with electrochemiluminescent immunoassay (ECLIA) method. Results: The measurement range of the assay was 2–940 ng/mL for CEA and 1.5–1000 ng/mL for AFP, with a detection limit of 0.94 ng/mL and 0.34 ng/mL, respectively. The inter-assay and intra-assay imprecision were all less than 6.58% and 10.62%, respectively. The RI of CEA and AFP was 0–3.84 ng/mL and 0–9.94 ng/mL, respectively. Regarding to clinical sample detection, no significant difference was observed between the proposed duplex assay and the ECLIA method. Conclusions: The ICP-MS-based duplex immunoassay was successfully developed and the analytical performance fully proved clinical applicability. Well, this could be different with other analytes.

scholarly journals Nanoparticle Characterisation of Traditional Homeopathically Manufactured Cuprum metallicum and Gelsemium sempervirens Medicines and Controls

Homeopathy ◽  
2018 ◽  
Vol 107 (04) ◽  
pp. 244-263 ◽  
Author(s):  
Martine Goyens ◽  
Etienne Capieaux ◽  
Philippe Devos ◽  
Pierre Dorfman ◽  
Michel Van Wassenhoven
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Pure Water ◽  
European Pharmacopoeia ◽  
Inductively Coupled ◽  
Significant Difference ◽  
Plasma Mass Spectrometry ◽  
The Mean ◽  
Gelsemium Sempervirens ◽  
Dilution Level

Background Homeopathy is controversial due to its use of very highly diluted medicines (high potencies/dynamisations). Methods We used a multi-technology approach to examine dilutions of two commonly used homeopathic medicines: an insoluble metal, Cuprum metallicum, and a soluble plant tincture, Gelsemium sempervirens, for the presence of nanoparticles (NPs) of original substance. The homeopathic medicines tested were specially prepared, according to the European pharmacopoeia standards. We compared the homeopathic dilutions/dynamisations with simple dilutions and controls. Results Using Mass Spectrometry (Single Particle-Inductively Coupled Plasma-Mass Spectrometry) and Dynamic Light Scattering (DLS) we could not find the expected copper in the 4cH potentisation and could not confirm the results previously obtained by Chikramane et al (2010). For Gelsemium medicines, using sensitive chromatography (HPLC-UV) up to a dilution level of 6 dH (3cH = dilution 10e-6), there was no significant difference in alkaloid content between a simple dilution and a homeopathic potency.For higher potentisations, however, NP tracking analysis findings revealed the presence of particles in all samples (except for pure water). The measurements showed large differences in particle quantities, mean particle sizes and standard deviations of the mean sizes between manufacturing lines of different starting material.There was always more material in potentised medicines than in potentised pure water. Gelsemium yielded the largest quantity of material (36 times more than that from copper at the same potentisation, 30 cH). The shapes and the chemical composition of the material are differentiable between different medicines and controls. Conclusion Potentisation influences specifically the nature of NPs detected. This material demonstrates that the step-by-step process (dynamised or not) does not match with the theoretical expectations in a dilution process. The Avogadro/Loschmidt limit is not relevant at all. It was not possible to reproduce the findings of Chikramane et al (2010) using inductively coupled plasma-mass spectrometry with copper. Copper NPs could not be detected at 4cH and above.

Using ICP-MS to Detect Inorganic Elements in Organic Materials: A New Tool to Identify Mordants or Dyes on Ancient Textiles

2004 ◽  
Vol 852 ◽  
Author(s):  
Laure Dussubieux ◽  
Mary W. Ballard
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Organic Materials ◽  
Organic Dye ◽  
Inorganic Elements ◽  
Metallic Elements ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Inorganic Constituents

ABSTRACTThe feasibility of determining metallic elements used as mordants or dyes with inorganic constituents was tested using inductively coupled plasma – mass spectrometry (ICP-MS). For this purpose, modern, historic and archaeological samples were investigated. Mordants containing copper, iron, tin, aluminium or uranium were successfully identified as well as an organic dye with a substitutional bromine.

scholarly journals Determination of Inorganic Elements in the Rhizome of Paris polyphylla Smith Var. chinensis (Franch.) Hara by Using Inductively Coupled Plasma Mass Spectrometry

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Tiezhu Chen ◽  
Juan Lin ◽  
Jun Yang ◽  
Yina Tang ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Limit Of Detection ◽  
Inorganic Elements ◽  
Cultivation Conditions ◽  
Limit Of Quantification ◽  
Inductively Coupled ◽  
Paris Polyphylla ◽  
Validation Parameters ◽  
Plasma Mass Spectrometry

The objective of this study was to investigate the concentrations of inorganic elements in the rhizome of Paris polyphylla Smith var. chinensis (Franch.) Hara of different planting years and cultivation conditions. Twenty-five inorganic elements including Al, As, B, Ba, Be, Bi, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Na, Ni, P, Pb, Se, Sr, Ti, V, and Zn in the rhizome were determined by using inductively coupled plasma mass spectrometry (ICP-MS). The analytical method was validated by measuring several parameters including linearity, correlation coefficient, limit of detection (LOD), limit of quantification (LOQ), and recovery. The linear working ranges were three, 0–300 μg/L, 0–500 μg/L, and 0–1000 μg/L, and the correlation coefficients (r) values were higher than 0.998. LOD varied from 0.001 μg/L (Be) to 11.957 μg/L (P), and LOQ varied from 0.003 μg/L (Be) to 35.870 μg/L (P). The recoveries spanned from 95.2 (Co) to 105.3% (Pb). Validation parameters showed the possibility of using whole of the sample preparation procedures used in this study. Based on the determined results, it is indicated that the toxic elements As, Cd, and Pb had no ingestion risk. The planting years and cultivation conditions had significant effects on the concentrations of inorganic elements of P. polyphylla var. chinensis. The concentrations of inorganic elements in cultivated samples were different from those in wild samples. The results can provide useful theoretical basis for the quality control and rational use of P. polyphylla var. chinensis.

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