scholarly journals Diagnostic Value of Cytokeratin 17 during Oral Carcinogenesis: An Immunohistochemical Study

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Sirima Sanguansin ◽  
Theerachai Kosanwat ◽  
Rachai Juengsomjit ◽  
Sopee Poomsawat
Keyword(s):  
Diagnostic Marker ◽  
Diagnostic Value ◽  
Premalignant Lesions ◽  
Oral Potentially Malignant Disorders ◽  
Normal Oral Mucosa ◽  
Cytokeratin 17 ◽  
Oral Carcinogenesis ◽  
Clinicopathologic Factors ◽  
Potentially Malignant

Background. Little is known about the role of cytokeratin 17 (CK17) during oral carcinogenesis. CK17 expression in oral leukoplakia (OL), the most encountered oral potentially malignant disorders and oral squamous cell carcinoma (OSCC), remains very limited. To determine the role of CK17 during oral carcinogenesis and its potential diagnostic marker in oral premalignant and malignant lesions, this study evaluated CK17 expression in OL without dysplasia, OL with dysplasia, and OSCC. CK17 expression in these tissues was compared with those of normal oral mucosa (NOM). Additionally, the relationship between CK17 expression and clinicopathologic factors of OSCC was investigated. Methods. CK17 expression was evaluated in 186 samples consisting of 12 NOM, 33 OL without dysplasia, 58 OL with dysplasia, and 83 OSCC using immunohistochemistry. The proportion of positively immunostained cells was evaluated and scored. Results. CK17 was expressed in 8.3%, 54.5%, 74.1%, and 90.4% of NOM, OL without dysplasia, OL with dysplasia, and OSCC, respectively. NOM had a significantly lower CK17 score than OL with dysplasia ( p = 0.0003 ) and OSCC ( p < 0.0001 ). A significant association between CK17 expression and histopathologic differentiation of OSCC was found. Tumors with well differentiation had high CK17 expression compared with those of moderate and poor differentiation. Conclusion. CK17 was overexpressed in OL with dysplasia and OSCC, suggesting that CK17 plays a pivotal role in the development of premalignant lesions and OSCC. Of clinical significance, CK17 may be a good diagnostic marker for oral premalignant lesions and OSCC. Additionally, CK17 could be used as an objective tool to classify histopathologic grade in OSCC. The findings that CK17 expression is high in OSCC but low in NOM imply that CK17 may serve as a potential therapeutic target for OSCC.

scholarly journals Clinical Review and Management of Oral Potentially Malignant Disorders with Epithelial Dysplasia

2021 ◽  
Author(s):  
Mustafa Mohammed Abdulhussain ◽  
Ali Sami Muhsin
Keyword(s):  
Oral Mucosa ◽  
Precancerous Lesion ◽  
Oral Lesion ◽  
Epithelial Dysplasia ◽  
Premalignant Lesions ◽  
Oral Potentially Malignant Disorders ◽  
Oral Epithelial Dysplasia ◽  
Normal Oral Mucosa ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

Background: Oral potentially malignant disorders (OPMDs) comprise any disorders, tumors, in addition to any microscopic alterations that have a risk of malignant development of cancers of the mouth. When epithelial dysplasia is detected in an oral lesion, it is termed as a precancerous lesion. Finding: Several changes in the color or thickness of normal oral mucosa might be detected during the clinical diagnosis of the oral lesions. Leukoplakia of the oral cavity is a clinical name for one of the most predominant OPMDs of the oral mucosa. When comparing oral examination with naked eyes to planning to apply staining with special stain or using an image of optical fluorescence, the incidence of patients with oral epithelial dysplasia may rise, as well as the clearing of the lesion boundary. Increased size of more than 2cm2, the presence of colored regions with a red hue, the presence of lichenoid process characteristics, and severe epithelial dysplasia are all considered risk factors. One-third of premalignant lesions may progress to cancer, whereas the other two-thirds may stay stable or regress without progressing to malignancy. Conclusion: It is critical to research the patients' unique characteristics, which include psychological, genetic, dietary, and dental problems. When epithelial dysplasia is present in an oral lesion, it is termed a precancerous lesion. Oral potential malignant diseases with epithelial dysplasia may or may not develop into carcinoma and may or may not be recurrent.

scholarly journals Clinical Review and Management of Oral Potentially Malignant Disorders with Epithelial Dysplasia

2021 ◽  
Vol 4 (4) ◽  
Author(s):  
Mustafa Mohammed Abdulhussain ◽  
◽  
Ali Sami Muhsin
Keyword(s):  
Oral Mucosa ◽  
Precancerous Lesion ◽  
Oral Lesion ◽  
Epithelial Dysplasia ◽  
Premalignant Lesions ◽  
Oral Potentially Malignant Disorders ◽  
Oral Epithelial Dysplasia ◽  
Normal Oral Mucosa ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

Background: Oral potentially malignant disorders (OPMDs) comprise any disorders, tumors, in addition to any microscopic alterations that have a risk of malignant development of cancers of the mouth. When epithelial dysplasia is detected in an oral lesion, it is termed as a precancerous lesion. Finding: Several changes in the color or thickness of normal oral mucosa might be detected during the clinical diagnosis of the oral lesions. Leukoplakia of the oral cavity is a clinical name for one of the most predominant OPMDs of the oral mucosa. When comparing oral examination with naked eyes to planning to apply staining with special stain or using an image of optical fluorescence, the incidence of patients with oral epithelial dysplasia may rise, as well as the clearing of the lesion boundary. Increased size of more than 2cm2, the presence of colored regions with a red hue, the presence of lichenoid process characteristics, and severe epithelial dysplasia are all considered risk factors. One-third of premalignant lesions may progress to cancer, whereas the other two-thirds may stay stable or regress without progressing to malignancy. Conclusion: It is critical to research the patients' unique characteristics, which include psychological, genetic, dietary, and dental problems. When epithelial dysplasia is present in an oral lesion, it is termed a precancerous lesion. Oral potential malignant diseases with epithelial dysplasia may or may not develop into carcinoma and may or may not be recurrent.

scholarly journals TNFα Signaling Is Increased in Progressing Oral Potentially Malignant Disorders and Regulates Malignant Transformation in an Oral Carcinogenesis Model

2021 ◽  
Vol 11 ◽  
Author(s):  
Jeffrey W. Chadwick ◽  
Rachel Macdonald ◽  
Aiman A. Ali ◽  
Michael Glogauer ◽  
Marco A. Magalhaes
Keyword(s):  
Malignant Transformation ◽  
Oncologic Outcomes ◽  
Oral Potentially Malignant Disorders ◽  
Critical Pathways ◽  
Oral Carcinogenesis ◽  
Multi Stage ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

Oral carcinogenesis represents a multi-stage process which encompasses several genetic and molecular changes that promote the progression of oral potentially malignant disorders (OPMDs) to oral squamous cell carcinomas (OSCCs). A better understanding of critical pathways governing the progression of OMPDs to OSCCs is critical to improve oncologic outcomes in the future. Previous studies have identified an important role of tumor necrosis factor α (TNFα) and TNF receptor 1 (TNFR1) in the invasiveness of oral cancer cell lines. Here, we investigate the expression of TNFα and TNFR1 in human OPMDs that progress to OSCC compared to non-progressing OPMDs utilizing fluorescent immunohistochemistry (FIHC) to show increased TNFα/TNFR1 expression in progressing OPMDs. In order to interrogate the TNFα/TNFR1 signaling pathway, we utilized a 4-nitroquinoline 1-oxide (4-NQO) mouse model of oral carcinogenesis to demonstrate that TNFα/TNFR1 expression is upregulated in 4-NQO-induced OSCCs. TNFα neutralization decreased serum cytokines, inhibited the development of invasive lesions and reduced tumor-associated neutrophils in vivo. Combined, this data supports the role of TNFα in oral malignant transformation, suggesting that critical immunoregulatory events occur downstream of TNFR1 leading to malignant transformation. Our results advance the understanding of the mechanisms governing OSCC invasion and may serve as a basis for alternative diagnostic and therapeutic approaches to OPMDs and OSCC management.

scholarly journals DNA Methylation Patterns in Saliva of Tobacco users with High-Risk Oral Potentially Malignant Disorders

2021 ◽  
pp. 129-137
Author(s):  
Mimansha Patel ◽  
Madhuri Nitin Gawande ◽  
Minal Shashikant Chaudhary ◽  
Alka Harish Hande
Keyword(s):  
Dna Methylation ◽  
High Risk ◽  
Malignant Transformation ◽  
Promoter Methylation ◽  
Genomic Dna ◽  
Premalignant Lesions ◽  
Oral Potentially Malignant Disorders ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Methylation Patterns

Background: “Oral Potentially Malignant Disorder (OPMD)” is a well-known symptom that, if untreated, can be carcinogenic. It includes leukoplakia, erythroplakia or erythroleukoplakia. One of the typical premalignant lesions of the oral cavity is “oral leukoplakias (OLs),” which frequently precedes “OSCCs.”OLs with dysplastic characteristics are considered to be at a higher risk of “malignant transformation.” So, early diagnosis of "oral squamous cell carcinomas (OSCCs)" is desperately required to enhance patient prognosis and quality of life (QOL).As a result, we examined the distinctive promoter methylation presence in high-risk OLs. Objectives: To detect, compare & correlate “DNA methylation” patterns in normal individuals, tobacco users without disease and tobacco users with the disease. Methodology: With the participants' full consent, 48 saliva samples were obtained and prepared. DNA isolation, restriction digestion of genomic DNA, extraction of restriction enzyme digested genomic DNA, Polymerase Chain Reaction (PCR), and Agarose Gel Electrophoresis (AGE) were all carried out. Expected results: This study will help us to assess the use of Saliva as an aid to identifying both high and low risk “Oral Potentially Malignant Disorders.” Conclusion: Peculiar promoter methylation of various genes was related to a high possibility of malignant transformation in OLs.

scholarly journals Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry

2010 ◽  
Vol 32 (5-6) ◽  
pp. 373-383
Author(s):  
Alessandra Donadini ◽  
Massimo Maffei ◽  
Antonio Cavallero ◽  
Monica Pentenero ◽  
Davide Malacarne ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Resolution ◽  
Human Lymphocytes ◽  
Mitotic Division ◽  
Dna Index ◽  
Normal Oral Mucosa ◽  
Oral Carcinogenesis ◽  
Clinically Normal ◽  
Risk Of Progression ◽  
Potentially Malignant

Oral potentially malignant lesions (OPMLs) with dysplasia and aneuploidy are thought to have a high risk of progression into oral squamous cell carcinomas (OSCCs). Non-dysplastic “oral distant fields” (ODFs), characterized by clinically normal appearing mucosa sited at a distance from co-existing OPMLs, and non-dysplastic OPMLs may also represent an early pre-cancerous state. ODFs, OPMLs without and with dysplasia and OSCCs were investigated by high resolution DNA content flow cytometry (FCM). ODFs and OPMLs without dysplasia were DNA aneuploid respectively in 7/82 (8.5%) and 25/109 (23%) cases. “True normal oral mucosa” and human lymphocytes from healthy donors were DNA diploid in all cases and were used as sex specific DNA diploid controls. Dysplastic OPMLs and OSCCs were DNA aneuploid in 12/26 (46%) and 12/13 (92%) cases. The DNA aneuploid sublines were characterized by the DNA Index (DI ≠ 1). Aneuploid sublines in ODFs and in non-dysplastic and dysplastic OPMLs were near-diploid (DI < 1.4) respectively in all, 2/3 and 1/3 of the cases. DNA aneuploid OSCCs, instead, were characterized prevalently by multiple aneuploid sublines (67%), which were commonly (57%) high-aneuploid (DI ≥ 1.4). DNA near-diploid aneuploid sublines in ODFs and OPMLs appear as early events of the oral carcinogenesis in agreement with the concept of field effect. Near-diploid aneuploidization is likely to reflect mechanisms of loss of symmetry in the chromosome mitotic division. High DNA aneuploid and multiple sublines in OPMLs with dysplasia and OSCCs suggest, instead, mechanisms of “endoreduplication” of diploid and near-diploid aneuploid cells and chromosomal loss. High resolution DNA FCM seems to enable the separation of subsequent progression steps of the oral carcinogenesis.

scholarly journals The role of interventional surgery in oral potentially malignant disorders

2014 ◽  
Vol 5 (2) ◽  
pp. 84-89 ◽  
Author(s):  
Peter Thomson
Keyword(s):  
Malignant Transformation ◽  
Scientific Literature ◽  
Squamous Carcinoma ◽  
Oral Potentially Malignant Disorders ◽  
Management Guidelines ◽  
Increased Risk ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Rising Incidence

Oral potentially malignant disorders are mucosal diseases with a significantly increased risk of squamous carcinoma development – a lethal and deforming disease with rising incidence, especially in young people. Despite the ability to recognise pre-cancer disorders in patients, clinicians remain unable to predict individual mucosal lesion behaviour or quantify the risk of malignant transformation. No clear management guidelines exist and the available scientific literature is unable to answer the fundamental question: does early diagnosis and interventional management treat pre-cancer effectively and prevent malignant transformation?

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