Characterization and Physical and Biological Properties of Tissue Conditioner Incorporated with Carum copticum L.

Aim. One of the main problems in dentistry is the injury caused by the long-term application of an ill-fitting denture. The existence of multiple microorganisms along with the susceptibility of the tissue conditioners to colonize them can lead to denture stomatitis. This study is aimed at developing a tissue conditioner incorporated with Carum copticum L. (C. copticum L.) for the effective treatment of these injuries. Materials and Methods. The Carum copticum L. essential oil composition was determined by gas chromatography-mass (GC-mass) spectrometry. The antimicrobial activity of the essential oil against the standard strains of bacterial and fungal species was determined by broth microdilution methods as suggested by the Clinical and Laboratory Standards Institute (CLSI). The physical and chemical properties of the prepared tissue conditioner were investigated by viscoelasticity, FTIR assays, and the release study performed. Furthermore, the antibiofilm activity of the Carum copticum L. essential oil-loaded tissue conditioner was evaluated by using the XTT reduction assay and scanning electron microscopy (SEM). Results. The main component of the essential oil is thymol, which possesses high antimicrobial activity. The broth microdilution assay showed that the essential oil has broad activity as the minimum inhibitory concentration was in the range of 32-128 μg mL-1. The viscoelasticity test showed that the essential oil significantly diminished the viscoelastic modulus on the first day. The FTIR test showed that Carum copticum L. essential oil was preserved as an independent component in the tissue conditioner. The release study showed that the essential oil was released in 3 days following a sustained release and with an ultimate cumulative release of 81%. Finally, the Carum copticum L. essential oil exhibited significant activity in the inhibition of microbial biofilm formation in a dose-dependent manner. Indeed, the lowest and highest amounts of biofilm formation on the tissue conditioner disks are exhibited in the Streptococcus salivarius and Candida albicans by up to 22.4% and 71.4% at the 64 μg mL-1 concentration of C. copticum L. with a statistically significant difference ( P < 0.05 ). Conclusion. The obtained results showed that the Carum copticum L. essential oil-loaded tissue conditioner possessed suitable physical, biological, and release properties for use as a novel treatment for denture stomatitis.

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Antimicrobial Essential Oil Combinations to Combat Foot Odour

Planta Medica ◽

10.1055/a-0592-8022 ◽

2018 ◽

Vol 84 (09/10) ◽

pp. 662-673 ◽

Cited By ~ 4

Author(s):

Ané Orchard ◽

Alvaro Viljoen ◽

Sandy van Vuuren

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Efficacy ◽

Juniperus Virginiana ◽

Broth Microdilution ◽

Synergistic Interactions ◽

Microdilution Method ◽

Broth Microdilution Method

AbstractFoot odour (bromodosis) is an embarrassing and perplexing condition mostly caused by bacteria of the Brevibacterium species. Essential oils are a credible option as an affordable treatment of odour and contribute towards antimicrobial efficacy. Therefore, this study sets out to investigate the antimicrobial activity of essential oil combinations against odour-causing bacteria. The broth microdilution method was used to investigate the antimicrobial activity of 119 essential oil combinations, and the fractional inhibitory index was calculated to determine the interactive profile. Combinations that resulted in synergy in 1 : 1 ratios were further evaluated in different concentrations, and isobolograms were plotted to determine the influence of the ratio on overall activity. Numerous combinations could be identified as having synergistic interactions against the Brevibacterium spp. and no antagonism was observed. The combination of Juniperus virginiana (juniper) and Styrax benzoin (benzoin) demonstrated synergy against all three Brevibacterium spp. tested and J. virginiana was the essential oil responsible for the majority of the synergistic interactions. The results reported here confirm the promising potential of the majority of these oils and selected combinations in treating and controlling bromodosis.

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Antimicrobial activity of Satureja hortensis L. essential oil against pathogenic microbial strains

Archives of Biological Sciences ◽

10.2298/abs1001159m ◽

2010 ◽

Vol 62 (1) ◽

pp. 159-166 ◽

Cited By ~ 20

Author(s):

Tatjana Mihajilov-Krstev ◽

Dragan Radnovic ◽

Dusanka Kitic ◽

Zorica Stojanovic-Radic ◽

Bojan Zlatkovic

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Wide Spectrum ◽

Significant Activity ◽

Fungal Strains ◽

Satureja Hortensis ◽

Conservation Treatment ◽

Microbial Strains ◽

Editorial Decision ◽

Font Color

A hydro-distilled oil of Satureja hortensis L. was investigated for its antimicrobial activity against a panel of 11 bacterial and three fungal strains. The antimicrobial activity was determined using the disk-diffusion and broth microdilution methods. The essential oil of S. hortensis L. showed significant activity against a wide spectrum of Gram (-) bacteria (MIC/MBC=0.025-0.78/0.05-0.78 ?l/ml) and Gram (+) bacteria (MIC/MBC=0.05-0.39/0.05-0.78 ?l/ml), as well as against fungal strains (MIC/MBC=0.20/0.78 ?l/ml). The results indicate that this oil can be used in food conservation, treatment of different diseases of humans, and also for the treatment of plants infected by phytopathogens. Detected autoplagiarism. Link to the Editorial Decision <a href="http://dx.doi.org/10.2298/ABS1004251U">10.2298/ABS1004251U</a>

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Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species

Journal of the Serbian Chemical Society ◽

10.2298/jsc091106052b ◽

2010 ◽

Vol 75 (6) ◽

pp. 739-747 ◽

Cited By ~ 27

Author(s):

Dinesh Bisht ◽

Rajendra Padalia ◽

Lalit Singh ◽

Veena Pande ◽

Priyanka Lal ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antagonistic Activity ◽

Significant Activity ◽

Variable Degree ◽

Bacterial Strains ◽

Fungal Strains ◽

Microbial Strains

The essential oils from six Himalayan Nepeta species, viz. Nepeta leucophylla Benth., Nepeta discolor Royle ex Benth., Nepeta govaniana Benth., Nepeta clarkei Hook f., Nepeta elliptica Royle ex Benth. and Nepeta erecta Benth., were tested for their in vitro antimicrobial activity against six pathogenic bacterial and two fungal strains. The results showed that Pseudomonas aeruginosa was the most sensitive strain tested to the essential oils of Nepeta species. The essential oils of N. elliptica and N. erecta exhibited the highest activity against P. aeruginosa, followed by the essential oils of N. leucophylla and N. clarkei. The essential oils from N. elliptica and N. erecta were also found to be very effective against Serratia marcescens; while the essential oil from N. leucophylla displayed significant activity against Proteus vulgaris and Staphylococcus aureus. Other bacterial strains displayed variable degree of susceptibility against one or more of the tested essential oils. The essential oil from N. leucophylla also showed the highest antifungal activity against both tested fungal strains, viz. Candida albicans and Trichophyton rubrum, followed by the essential oils from N. clarkei, N. govaniana and N. erecta. Iridodial derivatives, viz. iridodial ?-monoenol acetate (25.4 %), dihydroiridodial diacetate (18.2 %) and iridodial dienol diacetate (7.8 %) were identified as the major constituents of N. leucophylla, while the essential oils from N. elliptica and N. erecta were dominated by (7R)-trans, trans nepetalactone (83.4 %) and isoiridomyrmecin (66.7 %), respectively. The essential oil of N. discolor was characterized by 1,8-cineole (25.5 %) and ?-caryophyllene (18.6 %), while N. clarkei was dominated by ?-sesquiphellandrene (22.0 %) and germacrene D (13.0 %). Isoiridomyrmecin (35.2 %) and pregeijerene (20.7 %) were identified as the major constituents of N. govaniana. In general the Nepeta species containing constituents with an iridoid or lactone skeleton were found to have the greater antagonistic activity against most of the microbial strains as compared to those containing regular terpene constituents.

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HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil

Journal of the Serbian Chemical Society ◽

10.2298/jsc190513106s ◽

2019 ◽

Vol 84 (12) ◽

pp. 1355-1365 ◽

Cited By ~ 1

Author(s):

Jovana Stankovic ◽

Miroslav Novakovic ◽

Vele Tesevic ◽

Ana Ciric ◽

Marina Sokovic ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Inhibitory Activity ◽

Biofilm Formation ◽

Inhibitory Concentration ◽

Minor Component ◽

2D Nmr ◽

Antibacterial Compounds ◽

Direct Bioautography

This study was performed to determine the main antibacterial compounds of the essential oil (??) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC?MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations.

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Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

Journal of Chromatographic Science ◽

10.1093/chromsci/bmaa086 ◽

2020 ◽

Author(s):

Haïfa Debbabi ◽

Ridha El Mokni ◽

Ibrahim Jlassi ◽

Rajesh K Joshi ◽

Saoussen Hammami

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Properties ◽

Significant Activity ◽

Bacterial Strains ◽

Gram Positive Bacteria ◽

Sesquiterpene Hydrocarbons ◽

Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

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Juniperus communis essential oil limit the biofilm formation of Mycobacterium avium and Mycobacterium intracellulare on polystyrene in a temperature-dependent manner

International Journal of Environmental Health Research ◽

10.1080/09603123.2020.1741519 ◽

2020 ◽

pp. 1-14

Author(s):

Dolores Peruč ◽

Brigita Tićac ◽

Dalibor Broznić ◽

Željka Maglica ◽

Mladenka Šarolić ◽

...

Keyword(s):

Essential Oil ◽

Mycobacterium Avium ◽

Biofilm Formation ◽

Dependent Manner ◽

Temperature Dependent ◽

Juniperus Communis ◽

Mycobacterium Intracellulare

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Composition of Satureja kitaibelii Essential Oil and its Antimicrobial Activity

Natural Product Communications ◽

10.1177/1934578x1100600934 ◽

2011 ◽

Vol 6 (9) ◽

pp. 1934578X1100600 ◽

Cited By ~ 4

Author(s):

Tatjana Kundaković ◽

Marina Milenković ◽

Saša Zlatković ◽

Nada Kovačević ◽

Nikolić Goran

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Broth Microdilution ◽

Simple Formulation ◽

Aerial Parts ◽

Strong Antimicrobial Activity

The composition of the essential oil obtained by hydrodistillation from the aerial parts of Satureja kitaibelii from Rtanj mountain (Serbia), collected during three years, was studied. Thirty-nine components were identified in each sample of S. kitaibelii essential oil, representing about 87% of the oils. p-Cymene was the most dominant compound in all three oils (27.9%, 14.7% and 24.4%, respectively). The simple formulation of a lozenge with 0.2% of S. kitaibelii essential oil was prepared and the antimicrobial activity of the essential oil and the lozenge with essential oil was tested using a broth microdilution assay. Both essential oil and lozenge possessed strong antimicrobial activity with MIC values of 0.10-25 μg/mL, and 0.97-15.6 mg/mL, respectively.

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Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics

Natural Product Communications ◽

10.1177/1934578x1801300325 ◽

2018 ◽

Vol 13 (3) ◽

pp. 1934578X1801300 ◽

Cited By ~ 2

Author(s):

Marina Milenković ◽

Jelena Stošović ◽

Violeta Slavkovska

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Broth Microdilution ◽

Bacterial Strains ◽

Microdilution Method ◽

Broth Microdilution Method ◽

The Subject

The subject of the study was the investigation of the chemical composition and antimicrobial activity of the essential oils (EOs) isolated from Calamintha sylvatica, C. vardarensis, C. nepeta and C. glandulosa, as well as their antibacterial activity in combination with antibiotics. The quantitative and qualitative analysis of EOs was performed using the GC/FID and GC/MS methods. The antimicrobial activity of EOs against six standard bacterial strains and one strain of yeast was tested using the broth microdilution method, while the antimicrobial activity of a combination of essential oils and gentamicin/ciprofloxacin was tested by the checkerboard method. The dominant components (> 10%) of the essential oils were: cis-piperitone epoxide and menthone ( C. sylvatica), pulegone and menthone ( C. vardarensis), pulegone and piperitenone ( C. nepeta), pulegone, piperitenone, menthone and piperitone ( C. glandulosa). EOs did not exhibit significant antimicrobial activity except the essential oil of C. vardarensis which was selectively active against Staphylococcus aureus (MIC - 21.25 μg/mL). The overall effect of essential oil-antibiotic combinations varied from synergistic (FICI ≤ 0.5) to antagonistic (FICI ≥ 2) depending on the bacterial strain tested.

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Antimicrobial Activity of the Essential Oil ofPlectranthus neochilusagainst Cariogenic Bacteria

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/102317 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 19

Author(s):

Eduardo José Crevelin ◽

Soraya Carolina Caixeta ◽

Herbert Júnior Dias ◽

Milton Groppo ◽

Wilson Roberto Cunha ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Chemical Constituents ◽

Moderate Activity ◽

Significant Activity ◽

Streptococcus Sobrinus ◽

Cariogenic Bacteria ◽

Microdilution Method ◽

Broth Microdilution Method ◽

Major Chemical

This work used the broth microdilution method to investigate the antimicrobial activity of the essential oil obtained from the leaves ofPlectranthus neochilus(PN-EO) against a representative panel of oral pathogens. We assessed the antimicrobial activity of this oil in terms of the minimum inhibitory concentration (MIC). PN-EO displayed moderate activity againstEnterococcus faecalis(MIC = 250 μg/mL) andStreptococcus salivarus(MIC = 250 μg/mL), significant activity againstStreptococcus sobrinus(MIC = 62.5 μg/mL),Streptococcus sanguinis(MIC = 62.5 μg/mL),Streptococcus mitis(MIC = 31.25 μg/mL), andLactobacillus casei(MIC = 31.25 μg/mL), and interesting activity againstStreptococcus mutans(MIC = 3.9 μg/mL). GC-FID and GC-MS helped to identify thirty-one compounds in PN-EO;α-pinene (1, 14.1%),β-pinene (2, 7.1%),trans-caryophyllene (3, 29.8%), and caryophyllene oxide (4, 12.8%) were the major chemical constituents of this essential oil. When tested alone, compounds1,2,3, and4were inactive (MIC > 4000 μg/mL) against all the microorganisms. These results suggested that the essential oil extracted from the leaves ofPlectranthus neochilusdisplays promising activity against most of the evaluated cariogenic bacteria, especiallyS. mutans.

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Antimicrobial activity of some Essential oils and Extracts from Natural sources on Skin and Soft tissue infection causing microbes: An In-vitro Study

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00623 ◽

2021 ◽

pp. 3603-3609

Author(s):

Saumya Subramanian ◽

Padmaja Ananth Shenoy ◽

Vasudev Pai

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Soft Tissue ◽

Essential Oil ◽

Essential Oils ◽

Mentha Piperita ◽

Significant Activity ◽

Soft Tissue Infections ◽

Cinnamon Oil ◽

Cutibacterium Acnes

Introduction: Skin and soft tissue infections (SSTIs) constitute the diverse group of infections with varied clinical presentation and severity. SSTI’s are the most common infections treated in hospital today posing diagnostic and therapeutic challenges. Natural herbs have been widely used as a potential source of therapeutic substances all over the world. A study was conducted to determine the antimicrobial effect of some natural extracts on important SSTI causing pathogens. Materials and methods: In this study the antimicrobial potential of essential oil of Cinnamomum zeylanicum, Eugenia caryophyllata, oil of Cymbopogon citratus, Melaleuca alternifolia, Mentha piperita, Ocimum sanctum and extracts of Curcuma longa, Azadirachta indica and Cassia fistula were analysed against common skin and soft tissue infections (SSTIs) causing microorganisms. The organisms include methicillin resistant Staphylococcus aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA), Streptococcus pyogenes, Streptococcus agalactiae, Cutibacterium acnes, E.coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Bacteroides fragilis. The antimicrobial assay was performed by agar punch well method with different concentrations of essential oil and extracts. High performance liquid chromatography (HPLC) was performed in order to identify the active constituents present in the extracts. Further a formulation was made, with the powdered extract (neem, turmeric, bark of golden shower) and cinnamon oil as the chief component to look for any probable synergetic activity. Results and discussion: In this study all the essential oils showed good antimicrobial activity than the crude extracts. Significant activity against the Gram negative organisms was exhibited by all the products used in the study. Cinnamon and Clove oil had an excellent activity against all the organism whereas tea tree, mint and Tulsi oil showed better results against the Gram positive organisms including acne vulgaris causing organisms. HPLC results emphasised the presence of eugenol and cinnamaldehyde in clove and cinnamon oil respectively. A significant zone of inhibition was observed from the formulation prepared. Hence we indicate that the natural products used in this study have therapeutic properties and therefore could be considered as an alternative medication in treatment of SSTIs.

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