scholarly journals The Influence of ICAM1 3 ′ UTR Gene Polymorphism on the Occurrence and Metastasis of Primary Liver Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Li He ◽  
Suwen Wang ◽  
Xusheng Ma
Keyword(s):  
Primary Liver Cancer ◽  
Recessive Model ◽  
Primary Hepatocellular Carcinoma ◽  
Intercellular Adhesion Molecule ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dominant Model ◽  
Intercellular Adhesion Molecule 1 ◽  
Dominance Model ◽  
Increased Risk ◽  
History Of

Objective. In this study, we explored the influence of single nucleotide polymorphism (SNP) in the noncoding region of intercellular adhesion molecule 1 (ICAM1) gene on the occurrence and metastasis of primary hepatocellular carcinoma (PHC). Methods. Sanger sequencing was used to analyze the genotypes of rs3093032, rs923366, and rs281437 locus in the 3 ′ untranslated region (UTR) of the ICAM1 gene. The level of plasma ICAM1 was analyzed by enzyme-linked immunosorbent assay (ELISA). Results. After adjusting for risk factors such as BMI, smoking, drinking, family history of tumors, and hepatitis B virus test results, the CT genotype at rs3093032 of the ICAM1 gene ( OR = 0.19 , 95% CI: 0.08-0.44, P < 0.01 ), dominance model ( OR = 0.23 , 95% CI: 0.11-0.48, P < 0.01 ), and T allele ( OR = 0.27 , 95% CI: 0.14-0.53, P < 0.01 ) were related to the reduced risk of PHC susceptibility. rs923366 locus CT genotype ( OR = 0.63 , 95% CI: 0.44-0.90, P = 0.01 ), TT genotype ( OR = 0.23 , 95% CI: 0.10-0.53, P < 0.01 ), dominant model ( OR = 0.55 , 95% CI: 0.39-0.77, P < 0.01 ), recessive model ( OR = 0.28 , 95% CI: 0.12-0.62, P < 0.01 ), and T allele ( OR = 0.55 , 95% CI: 0.42-0.73, P < 0.01 ) were related to a reduction in the risk of PHC susceptibility. rs281437 locus CT genotype ( OR = 2.08 , 95% CI: 1.40-3.09, P < 0.01 ), TT genotype ( OR = 5.20 , 95% CI: 2.22-12.17, P < 0.01 ), dominant model ( OR = 2.45 , 95% CI: 1.69-3.54, P < 0.01 ), recessive model ( OR = 4.32 , 95% CI: 1.86-10.06, P < 0.01 ), and T allele ( OR = 2.46 , 95% CI: 1.79-3.38, P < 0.01 ) were significantly related to the increased risk of PHC susceptibility. SNPs at rs3093032, rs923366, and rs281437 of the ICAM1 gene were significantly correlated with TNM stage and tumor metastasis of PHC patients ( P < 0.05 ). Conclusion. SNPs at rs3093032, rs923366, and rs281437 in the 3 ′ UTR region of the ICAM1 gene are related to the occurrence and metastasis of PHC.

scholarly journals VCAM-1 Is Upregulated in Uranium Miners Compared to Other Miners

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1223
Author(s):  
Nour A. Ass’ad ◽  
Xin Shore ◽  
Orrin Myers ◽  
Alexandra R. Camacho ◽  
Quiteria Jacquez ◽  
...  
Keyword(s):  
Adhesion Molecule ◽  
Multivariable Analysis ◽  
The United States ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Metal Mining ◽  
Amyloid A ◽  
History Of ◽  
Screening Clinic ◽  
Mining Coal

The United States has a rich history of mining including uranium (U)-mining, coal mining, and other metal mining. Cardiovascular diseases (CVD) are largely understudied in miners and recent literature suggests that when compared to non-U miners, U-miners are more likely to report CVD. However, the molecular basis for this phenomenon is currently unknown. In this pilot study, a New Mexico (NM)-based occupational cohort of current and former miners (n = 44) were recruited via a mobile screening clinic for miners. Serum- and endothelial-based endpoints were used to assess circulating inflammatory potential relevant to CVD. Non-U miners reported significantly fewer pack years of smoking than U-miners. Circulating biomarkers of interest revealed that U-miners had significantly greater serum amyloid A (SAA), soluble intercellular adhesion molecule 1 (ICAM-1, ng/mL), soluble vascular cell adhesion molecule 1 (VCAM-1, ng/mL), and VCAM-1 mRNA expression, as determined by the serum cumulative inflammatory potential (SCIP) assay, an endothelial-based assay. Even after adjusting for various covariates, including age, multivariable analysis determined that U-miners had significantly upregulated VCAM-1 mRNA. In conclusion, VCAM-1 may be an important biomarker and possible contributor of CVD in U-miners. Further research to explore this mechanism may be warranted.

scholarly journals Circulating cell adhesion molecules in metabolically healthy obesity

2020 ◽  
Author(s):  
Arij Mulhem ◽  
Yusef Moulla ◽  
Nora Klöting ◽  
Thomas Ebert ◽  
Anke Tönjes ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecules ◽  
Intercellular Adhesion Molecule ◽  
Serum Concentrations ◽  
Metabolically Healthy Obesity ◽  
Intercellular Adhesion Molecule 1 ◽  
Increased Risk ◽  
Metabolically Healthy

Abstract Background/Objectives People with metabolically healthy obesity (MHO) may still have an increased risk for cardiovascular mortality compared to metabolically healthy lean (MHL) individuals. However, the mechanisms linking obesity to cardiovascular diseases are not entirely understood. We therefore tested the hypothesis that circulating cell adhesion molecules (CAMs) are higher in MHO compared to MHL individuals. Subjects/Methods Serum concentrations of soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular adhesion molecule-1 (sVCAM-1), E-selectin and P-selectin were measured in age- and sex-matched groups of MHL (n = 32), MHO categorized into BMI-matched insulin sensitive (IS, n = 32) or insulin resistant (IR) obesity (n = 32) and people with metabolically unhealthy obesity (MUO, n = 32). Results Indeed, individuals with MHO have significantly higher sICAM-1, E-selectin, and P-selectin serum concentrations compared to MHL people. However, these CAMs are still significantly lower in IS compared to IR MHO. There was no difference between the groups in sVCAM-1 serum concentrations. Compared to all other groups, circulating adhesion molecules were significantly higher in individuals with MUO. Conclusions These findings suggest that obesity-related increased cardiovascular risk is reflected and may be mediated by significantly higher CAMs. The mechanisms causing elevated adhesion molecules even in the absence of overt cardio-metabolic risk factors and whether circulating CAMs could predict cardiovascular events need to be explored.

Circulating forms of intercellular adhesion molecule (ICAM)-1 in mice lacking membranous ICAM-1

Blood ◽  
2000 ◽  
Vol 95 (4) ◽  
pp. 1350-1355 ◽  
Author(s):  
Natasja K. van den Engel ◽  
Edmund Heidenthal ◽  
Antje Vinke ◽  
Hubert Kolb ◽  
Stephan Martin
Keyword(s):  
Adhesion Molecule ◽  
Transmembrane Domain ◽  
Flow Cytometric Analysis ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Functions ◽  
Wild Type ◽  
Spleen Cells ◽  
Intercellular Adhesion Molecule 1

Mice deficient in intercellular adhesion molecule-1 (ICAM-1), lacking membranous ICAM-1, show a normal development but abnormalities of inflammatory and immune functions. Although the membrane-bound form of ICAM-1 is not detectable in the mutant strain, circulating ICAM-1 (cICAM) is present in serum from ICAM-1-deficient mice in similar amounts as in serum from wild-type mice. These findings were confirmed in vitro by flow cytometric analysis of lipopolysaccharide-stimulated spleen cells, and cICAM-enzyme-linked immunosorbent assay analysis of supernatants of cultured spleen cells. To analyze for the source of cICAM-1, spleen cell RNA was isolated and ICAM-1 RNA was amplified by reverse transcriptase-polymerase chain reaction using primers binding in the 5′ and 3′ untranslated regions. Different fragments were cloned and sequenced. In wild-type RNA the common 5 domain form of ICAM-1 was identified. In RNA from ICAM-1 mutant mice only 3 smaller fragments were found. Sequencing these fragments identified 3 alternatively spliced isoforms of ICAM-1, lacking 2 or 3 extracellular domains. However, in all spliced fragments the transmembrane domain was included. Therefore, we postulate that circulating forms of ICAM-1 are generated by proteolytic cleavage of membranous ICAM-1. The data indicate that the expression of membranous ICAM-1 and the appearance of circulating forms in serum are independently regulated mechanisms.

Increased Serum Concentrations of Adhesion Molecules after Coronary Angioplasty

1994 ◽  
Vol 87 (6) ◽  
pp. 627-633 ◽  
Author(s):  
Robert W. Kurz ◽  
Bernhard Graf ◽  
Franz Gremmel ◽  
Christian Wurnig ◽  
Felix Stockenhuber
Keyword(s):  
Coronary Angiography ◽  
Adhesion Molecules ◽  
Adhesion Molecule ◽  
Coronary Angioplasty ◽  
Interleukin 2 ◽  
Intercellular Adhesion Molecule ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Concentrations ◽  
Intercellular Adhesion Molecule 1

1. Reocclusion is still a significant complication after percutaneous transluminal coronary angioplasty. The injury of coronary arteries resulting from PTCA plays an important role in the pathophysiology of both abrupt closure and late restenosis after an initially successful procedure. Cytokines play a pivotal role in the accumulation of circulating blood cells at the endothelium and are known to regulate their interaction with the vessel wall. 2. To obtain further information about this interaction, serum concentrations of soluble endothelial leukocyte adhesion molecule 1 (sELAM-1), leucocyte endothelial cell adhesion molecule 1 (sL-selectin), intercellular adhesion molecule 1 (sICAM-1), interleukin 2 receptor (sIL-2R) and interleukin 8 (IL-8) detected by enzyme-linked immunosorbent assay were monitored in 30 consecutive patients referred for elective PTCA. Fifteen patients who underwent elective coronary angiography without PTCA served as controls. 3. All patients underwent successful first PTCA. Within 24 h the serum concentrations of sELAM-1 increased gradually from 21.7 (SD 7.1) to 48.2 (SD 8.6) ng/ml (P < 0.01); levels of sL-selectin rose from 982.1 (SD 128.7) to 1541.3 (SD 104.6) ng/ml after 48h (P < 0.01). Serum levels of IL-8 remained stable initially, but peaked at the end of the observation time of 72 h (9.4, SD 3.8, versus 16.1, SD 4.9 ng/ml; P < 0.05). A positive correlation was found between the number of dilatations and the rise in these parameters (P < 0.01). No significant changes were found in the serum concentrations of sICAM-1 and sIL-2R after PTCA or in any of the parameters in patients after coronary angiography. 4. We conclude that PTCA induces a significant rise in the concentration of certain adhesion molecules in serum. Thus, we provide preliminary data on the potential role of cytokines for blood cell-endothelium interaction after PTCA. Further investigations and larger numbers of patients are needed to clarify the role of circulating cytokines for endothelial injury and restenosis after PTCA.

scholarly journals 5-HTTLPR polymorphism in the serotonin transporter gene and migraine: A systematic review and meta-analysis

Cephalalgia ◽  
2010 ◽  
Vol 30 (11) ◽  
pp. 1296-1305 ◽  
Author(s):  
Markus Schürks ◽  
Pamela M Rist ◽  
Tobias Kurth
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Recessive Model ◽  
Dominant Model ◽  
Migraine Aura ◽  
Transporter Gene ◽  
Increased Risk ◽  
Genotype Information ◽  
S Allele ◽  
European Women

Background and methods: Data on the association between the SLC6A4 5-HTTLPR polymorphism and migraine are conflicting. We performed a systematic review and meta-analysis among studies published up to September 2009. For each study with genotype information, we calculated odds ratios (OR) and 95% confidence intervals (CI) assuming additive, dominant, and recessive genetic models. We then calculated pooled ORs and 95% CIs. Results: Among the ten studies identified there was no overall association between the polymorphism and any migraine for Europeans or Asians. However, European women carrying the S allele had an increased risk for any migraine (dominant model: pooled OR = 2.02; 95% CI 1.24–3.28). Results among Europeans further suggested an increased risk for migraine with aura among carriers of the S/S genotype (recessive model: pooled OR = 1.41; 95% CI 0.83–2.40). Conclusions: While our results indicate no overall association between the SLC6A4 5-HTTLPR polymorphism and migraine among Europeans and Asians, gender and migraine aura status may have modifying roles among Europeans.

Baseline ICAM-1 and VCAM-1 are Increased in Initially Healthy Middle-Aged Men who Develop Cardiovascular Disease during 6.6 Years of Follow-Up

Angiology ◽  
2008 ◽  
Vol 60 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Caroline Schmidt ◽  
Johannes Hulthe ◽  
Björn Fagerberg
Keyword(s):  
Adhesion Molecule ◽  
Femoral Artery ◽  
Cardiovascular Events ◽  
High Serum ◽  
Intercellular Adhesion Molecule ◽  
Serum Concentrations ◽  
Intercellular Adhesion Molecule 1 ◽  
Baseline Serum ◽  
Increased Risk

The objective of the present study was to investigate if there was a difference in baseline serum concentrations of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) between groups with and without cardiovascular events during a mean follow-up of 6.6 years in a group of initially healthy 58-year-old men. A further aim was to examine if high serum concentrations of ICAM-1 and VCAM-1 were associated with carotid and femoral artery plaque occurrence, separately. Men with cardiovascular events during follow-up had higher median serum ICAM-I and VCAM-I than those without events ( P < .05). The median of serum ICAM-I and VCAM-1 in the event group was used as the cutoff level, and in those with ICAM-1 and VCAM-1 above the cutoff value, there was an increased risk of having a plaque in the femoral artery (OR = 2.8, 95% CI = 1.8-4.3; and OR = 1.6, 95% CI = 1.1-2.5, respectively).

scholarly journals Meta-Analysis of the Relationship between XRCC1-Arg399Gln and Arg280His Polymorphisms and the Risk of Prostate Cancer

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jie Yan ◽  
Xiantao Wang ◽  
Hui Tao ◽  
Zengfu Deng ◽  
Wang Yang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Risk ◽  
Meta Analysis ◽  
Prostate Cancer Risk ◽  
Recessive Model ◽  
Dominant Model ◽  
Xrcc1 Arg399gln ◽  
Increased Risk ◽  
Arg399gln Polymorphism ◽  
The Relationship

Abstract Prostate cancer is one of the most common noncutaneous malignancies in Western countries. Because there has been a debate regarding the relationship between the XRCC1-Arg399Gln and Arg280His polymorphisms and prostate cancer risk, we therefore performed this meta-analysis. The electronic databases PubMed, EMBASE and Medline were searched prior to October 1, 2014. An odds ratio and 95% confidence interval were used to calculate association. Heterogeneity was tested by both a chi-square test and I2statistic. Funnel plots and Egger’s test were used to assess publication bias. All statistical analyses were performed using STATA 12.0 software. A significant association between the XRCC1-Arg399Gln polymorphism and prostate cancer risk was found under a homozygote model and a recessive model. A significant association between XRCC1-Arg280His and prostate cancer risk was found under a heterozygote model and a dominant model. Overall, the results of this meta-analysis show that the XRCC1-Arg399Gln polymorphism may be associated with an increased risk for prostate cancer under the homozygote model and the recessive model. And XRCC1-Arg280His polymorphism is likely to be related with prostate cancer risk under the heterozygote model and the dominant model. Additional larger well-designed studies are needed to validate our results.

scholarly journals Early childhood infections, antistreptococcal and basal ganglia antibodies in adult ADHD: A preliminary study

2020 ◽  
Author(s):  
Francesco Oliva ◽  
Giulia di Girolamo ◽  
Francesca Malandrone ◽  
Noemi Iaia ◽  
Fiorella Biasi ◽  
...  
Keyword(s):  
Basal Ganglia ◽  
Adult Adhd ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recurrent Infections ◽  
Hyperactivity Disorder ◽  
Childhood Infections ◽  
Streptolysin O ◽  
Increased Risk ◽  
History Of ◽  
The Relationship

Abstract Background: To explore the relationship between adult Attention Deficit/ Hyperactivity Disorder (ADHD), antistreptococcal titers, ABGA, and recurrent infections during early childhood.Method: Childhood history of recurrent infections and a blood sample were collected in a sample of DSM-IV adult outpatients with ADHD. The anti-streptolysin O (ASO), anti-deoxyribonuclease B (anti-DNase B), and anti-basal ganglia antibodies (ABGA) titers were determined in patient plasma by enzyme-linked immunosorbent assay (ELISA). Titers positivity was evaluated following manufacturer's specifications. Absolute titers were also collected as continuous variables.Results: Fourteen out of 22 (63.6%) have had recurrent infections in childhood (i.e., seven, 31.8%, have had tonsillitis or adenoiditis and seven, 31.8%, have had any other infections). Eighteen patients (81.9%) were positive for anti-DNase B, five (22.7%) for ASO, and 4 (18.2%) were positive for both of them. Five participants (22.7%) were ABGA positive, whereas only two (9.1%) were positive for all three antibodies.Conclusions: patients with ADHD might be more prone to infections during childhood and subclinical streptococcal infections during adulthood. Moreover, they seem to have an increased risk for basal ganglia autoimmunity in adulthood. Both infections and the ensuing acquired autoimmunity could influence the neurodevelopmental process, by contributing, at least in part, to the ADHD pathogenesis.

Sign in / Sign up

Export Citation Format

Share Document