Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics

Context. The wettability of the chemically modified dentin substrate is a condition that intervenes in dentin-vascular and cellular interaction across regenerative endodontics. Aims. To compare the effect of CS and CSnp on the wettability in root dentine with other irrigation protocols with an experimental in vitro model prior regenerative endodontics. Methods and Material. An in vitro experimental study that included eighty hemisected human root distributed into 8 groups: G1- distilled water; G2- 1% NaOCl/17% EDTA; G3- hypochlorous acid 0.025% HOCl, G4- 1% NaOCl/0.025% HOCl/17% EDTA, G5- 0.2 g/100 mL CS, G6- 1% NaOCl/0.2 g/100 mL CS, G7- CSnp, and G8- 1% NaOCl/CSnp. The wettability analysis calculated the contact angle (θ) between a drop of a blood-like and root dentinal surface; topographic characterization with scanning electron microscopy (SEM) quantified the diameter and number of tubules per area; spectroscopy infrared analyses (IR-S) identified chemical changes in the inorganic (phosphate/carbonate) and organic phase (amide/methyl). Statistical analysis: a linear mixed model, Kruskal–Wallis, and Holm–Bonferroni correction ( P < 0.05) were used. Results. Significantly higher wettability for G2 (27.1 ( P = 0.0001)) was found. A mean value of 67°±°for experimental groups ( P = 0.07) was found, and we did not identify differences between them. The SEM identified greater tubular opening and erosion for G4 and greater dentinal permeability per area for NaOCl/CS. IR-S identified dentinal organic integrity with NaOCl-CS/CSnp compared to organic reduction promoted for NaOCl/EDTA. Conclusions. This in vitro dentin determined an indirect association between the wettability and organic contents. The oxidative effect of NaOCl could be neutralized by CS-CSnp, and consequently, the wettability of the substrate decreases.

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Development of a Platform of Cellular Interaction in Tissue Patterns: In Vitro Model of Endomycardial Fibrosis

2019 Global Medical Engineering Physics Exchanges/ Pan American Health Care Exchanges (GMEPE/PAHCE) ◽

10.1109/gmepe-pahce.2019.8717360 ◽

2019 ◽

Author(s):

P. Orozco ◽

Y. Montoya ◽

J. Bustamante

Keyword(s):

In Vitro Model ◽

Cellular Interaction ◽

Vitro Model

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Comment on the paper, “Growth of yeast cultures as in vitro model for investigating homœopathic remedies”

British Homeopathic Journal ◽

10.1016/s0007-0785(85)80042-9 ◽

1985 ◽

Vol 74 (02) ◽

pp. 93-96 ◽

Cited By ~ 4

Author(s):

Rose D. Baker ◽

C.W. Smith

Keyword(s):

Saccharomyces Cerevisiae ◽

Growth Rate ◽

In Vitro Model ◽

Mean Value ◽

Statistical Test ◽

Yeast Cultures ◽

The Mean ◽

Vitro Model

SummaryA recent paper by Steffen1 repeating earlier work by Jones et al.2 failed to confirm any effect of potencies of Pulsatilla on the growth rate of cultures of Saccharomyces cerevisiae which had previously been reported.We find that using a more specific statistical test on the results given by Steffen1 it can be shown that these results contain almost the same periodicity with potency that Jones et al.2 reported, although only corresponding to a variation of ±1.5% about the mean value.

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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