Chemical Composition and Biological Activities of the Essential Oils of Chrysophyllum albidum G. Don (African Star Apple)

The volatile compounds of the fruit and leaf essential oils of the African star fruit, Chrysophyllum albidum G. Don, were characterized by gas chromatography-mass spectrometry in this study. The antimicrobial, antibiofilm, and antioxidant activities of the essential oils were also investigated. Thirty-five and thirty-four compounds, representing 97.84% and 97.87%, were identified in the leaf and fruit essential oils, respectively. The antimicrobial activity of the oils was evaluated in vitro against eight pathogens using the broth microdilution method. The fruit essential oil exhibited broad-spectrum antimicrobial activity in the antimicrobial susceptibility test, with minimum inhibitory concentrations (MICs) ranging from 0.195 to 6.250 mg/mL, while the leaf essential oils showed antimicrobial activity with MICs in the range of 6.875–13.750 mg/mL. The antibiofilm activity was assessed via the crystal violet staining assay, with Pseudomonas aeruginosa as the model organism. The concentrations of the leaf and fruit essential oil required for half-maximal inhibition of biofilm formation (BIC50) were 6.97 ± 0.56 and 4.78 ± 0.21 mg/mL, respectively. In evaluating antioxidant activity, the total antioxidant capacity obtained from the phosphomolybdenum assay was 104.8 ± 2.4 and 101.6 ± 0.8 μg/g AAE for leaf and fruit essential oils, respectively. The IC50 values obtained from the hydrogen peroxide scavenging, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and inhibition of lipid peroxidation assays were 301.8 ± 0.7 and 669.2 ± 2.1 μg/mL, 1048.0 ± 0.3 and 1454.0 ± 0.3 μg/mL, and 460.1 ± 2.7 and 457.4 ± 0.3 μg/mL for both leaf and fruit essential oils, respectively. The results obtained in this study suggest that the leaf and fruit essential oil of Chrysophyllum albidum G. Don could find potential use in the food, cosmetic, and pharmaceutical industries as preservative and pharmaceutical agents.

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Chemical Composition and Biological Activities of Essential Oils from the Leaves, Stems, and Roots of Kadsura coccinea

Molecules ◽

10.3390/molecules26206259 ◽

2021 ◽

Vol 26 (20) ◽

pp. 6259

Author(s):

Tianming Zhao ◽

Chao Ma ◽

Guofei Zhu

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oils ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging ◽

Cholinesterase Inhibition ◽

Pharmaceutical Industries ◽

Kadsura Coccinea ◽

Radical Scavenging Assay

The chemical composition and biological activities of the essential oils from the leaves, stems, and roots of Kadsura coccinea (K. coccinea) were investigated. The essential oils were extracted by hydro distillation and analyzed by gas chromatography mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). Antioxidant activities of the essential oils were examined with DPPH radical scavenging assay, ABTS cation radical scavenging assay, and ferric reducing antioxidant power assay. Antimicrobial activities were evaluated by determining minimum inhibitory concentrations (MIC) and minimum microbiocidal concentrations (MMC). Acetylcholinesterase and butyrylcholinesterase inhibitory activity of the essential oils were also tested. A total of 46, 44, and 47 components were identified in the leaf, stem, and root oils, representing 95.66%, 97.35%, and 92.72% of total composition, respectively. The major compounds of three essential oils were α-pinene (16.60–42.02%), β-pinene (10.03–18.82%), camphene (1.56–10.95%), borneol (0.50–7.71%), δ-cadinene (1.52–7.06%), and β-elemene (1.86–4.45%). The essential oils were found to have weak antioxidant activities and cholinesterase inhibition activities. The essential oils showed more inhibitory effects against Staphylococcus aureus (S. aureus) than those of other strains. The highest antimicrobial activity was observed in the root oil against S. aureus, with MIC of 0.78 mg/mL. Therefore, K. coccinea essential oils might be considered as a natural antibacterial agent against S. aureus with potential application in food and pharmaceutical industries.

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Chemical composition, antimicrobial and antioxidant properties of Mentha longifolia (L.) Huds. essential oil

Journal of Health Sciences ◽

10.17532/jhsci.2012.38 ◽

2012 ◽

Vol 2 (3) ◽

pp. 192-200 ◽

Cited By ~ 11

Author(s):

Haris Nikšić ◽

Elvira Kovač Bešović ◽

Elma Makarević ◽

Kemal Durić

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Radical Scavenging Capacity ◽

Scavenging Capacity ◽

Mentha Longifolia ◽

Layer Chromatography

Introduction: Present study describes the antimicrobial activity and free radical scavenging capacity (RSC) of essential oil from Mentha longifolia (L.) Huds. Aim of this study to investigate the quality, antimicrobial andantioxidant activity of wild species Mentha longifolia essential oil from Bosnia and Herzegovina.Methods: The chemical profi le of essential oil was evaluated by the means of gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC). Antimicrobial activity was tested against 6bacterial strains. RSC was assessed by measuring the scavenging activity of essential oils on 2,2- diphenyl-1-picrylhydrazil (DPPH).Results: The main constituents of the essential oil of M. longifoliae folium were oxygenated monoterpenes,piperitone oxide (63.58%) and 1,8-cineole (12.03%). Essential oil exhibited very strong antibacterial activity.The most important antibacterial activity essential oil was expressed on Gram negative strains: Escherichia coli, Pseudomonas aerginosa and Salmonella enterica. subsp.enterica serotype ABONY. Antioxidant activity was evaluated as a RSC. Investigated essential oil was able to reduce DPPH radicals into the neutral DPPHH form (IC50=10.5 μg/ml) and this activity was dose –dependent.Conclusion: The study revealed signifi cant antimicrobial activity of the investigated essential oil. The examined oil exhibited high RSC, which was found to be in correlation to the content of mainly monoterpeneketones and aldehydes. These results indicate that essential oils could serve as safe antioxidant and antiseptic supplements in pharmaceuticals.

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Studies on the Antimicrobial and Antioxidant Activity and Chemical Composition of the Essential Oils of Kitaibelia vitifolia

Natural Product Communications ◽

10.1177/1934578x1300800530 ◽

2013 ◽

Vol 8 (5) ◽

pp. 1934578X1300800 ◽

Cited By ~ 1

Author(s):

Pavle Mašković ◽

Marija Radojković ◽

Mihailo Ristić ◽

Slavica Solujić

Keyword(s):

Lipid Peroxidation ◽

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Metal Chelating

The objective of this study was to evaluate the potential use of the essential oil of Kitaibelia vitifolia Willd. in the pharmaceutical and food industries. Antimicrobial and antioxidant activities of essential oil of K. vitifolia isolated by hydrodistillation using a Clevenger-type apparatus were assessed. GC/FID and GC/MS analyses were used to determine the major components of the essential oil as sclareoloxide ( cis A/B) 17.9%, sclaral 10.9%, labda-7,13,14-triene 10.6% and sclareol 9.5%. The antimicrobial activity of the essential oil was evaluated against the bacterial strains: Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae ATCC 13883, Escherichia coli ATCC 25922, Proteus vulgaris ATCC 13315, P. mirabilis ATCC 14153, and Bacillus subtilis ATCC 6633; and fungal strains: Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404. Antimicrobial activity was tested using a broth dilution procedure for determination of minimum inhibitory concentration (MIC). The essential oil of K. vitifolia showed strong antimicrobial activity. Antioxidant activities of the essential oils were evaluated using the DPPH and hydroxy radical scavenging, lipid peroxidation and metal chelating assays. The results for antioxidant activity were compared with control antioxidants, ascorbic acid, gallic acid, α-tocopherol and BHT. Results showed that the essential oil possesses antioxidant activity, with total antioxidant capacity of 95.4±0.7 μg AA/g and IC50 values of 5.45±1.45 μg/mL for DPPH free radical scavenging activity, 26.5±1.6 μg/mL for inhibitory activity against lipid peroxidation, 79.4±0.4 μg/mL for hydroxyl radical scavenging activity, and 39.9±0.7 μg/mL for metal chelating activity.

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Composition of essential oil and antioxidant activity of Khat (Catha edulis Forsk), Ethiopia

10.31221/osf.io/w7h8j ◽

2019 ◽

Author(s):

Chem Int

Keyword(s):

Gas Chromatography ◽

Essential Oils ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Bahir Dar ◽

Dpph Radical ◽

Catha Edulis

In this study, we determined the chemical composition and antioxidant activities of the essential oils from two different varieties of khat (Catha edulis Forsk) cultivated in Ethiopia. The essential oils were extracted by hydrodistillation using the Clevenger type apparatus, identifications of compounds were made by gas chromatography and gas chromatography-mass spectrometry (GC-MS). Seventy seven different compounds were identified from essential oils of the two different khat cultivars. The essential oils in the samples from Bahir Dar and Wendo were composed of 50 and 34 compounds, respectively. The major compound identified in khat essentials oils include: limonene, 1-phenyl-1,2-propanedione, 1-hydroxy,1-phenyl-2-propanone, camphor, (sulfurous acid)-2-propylundecyl ester, hexadecane, O-mentha-1(7), 8-dien-3-ol, heptadecane, 10-methylnonadecane, (phthalic acid)-isobutyl octadecyl ester, and tritetracontane. The antioxidant and free radical scavenging activity of the oils were assessed by means of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay. The scavenging activities of the oils were 23.5-23.6 μg AAE/kg of fresh khat sample.

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Chemical variability and antioxidant activities of the essential oils of the aerial parts of Ammoides verticillata and the roots of Carthamus caeruleus and their synergistic effect in combination

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207323666200606213057 ◽

2020 ◽

Vol 23 ◽

Author(s):

Imane Rihab Mami ◽

Noria Merad-Boussalah ◽

Mohammed El Amine Dib ◽

Boufeldja Tabti ◽

Jean Costa ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Natural Food ◽

Chemical Compositions ◽

Chemical Variability ◽

Ammoides Verticillata

Aim and Objective: Oxidative stress is implicated in the development and progression of many disease. Some of appropriate actions that could be initiated to taken to resolve the problem of these diseases are search for new antioxidant substances isolated from plants. The aims of this study were to study the intraspecies variations of A. verticillata and C. caeruleus essential oils from 8 locations using statistical analysis, the in vitro antioxidant properties of collective essential oils and in combinations. Materials and Methods: The essential oils were analyzed by GC and GC-MS. The intraspecies variations of the essential oil compositions were discussed using principal component analysis (PCA) and cluster analysis (CA). The antioxidant properties were evaluated DPPH-radical scavenging activity and β-carotene bleaching test. Results: The main components of Ammoides verticillata collective essential oil (Coll EO) were thymol (30.5%), carvacrol (23.2%), p-cymene (13.1%), limonene (12.5%) and terpinene-4-ol (12.3%). While roots of Carthamus caeruleus essential oil were dominated by carline oxide (86.2%). The chemical variability allowed the discrimination of two main Groups for both Coll EOs. A direct correlation between the altitudes, climate and the chemical compositions was evidenced. Ammoides verticulata and Carthamus caeruleus Coll Eos showed good antioxidant activity. In binary mixture, the interaction both Coll Eos and between oils rich of thymol and/or carvacrol with carlina oxide produced the best synergistic effects, compared to individual essential oils and the synthetic antioxidant (BHT). Conclusion: Ammoides verticillata and Carthamus caeruleus essential oil blends can be used as a natural food preservative and alternative to chemical antioxidants.

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Evaluation of antioxidant and antimicrobial activity of Ziziphora ‎clinopodiodes essential oil eluted at different times

Current Bioactive Compounds ◽

10.2174/1573407216999200507122039 ◽

2020 ◽

Vol 16 ◽

Author(s):

Mojgan Alizadeh ◽

Akram Arianfar ◽

Ameneh Mohammadi

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Positive Control ◽

Essential Oil Composition ◽

Oil Composition ◽

Citronellic Acid ◽

Antimicrobial And Antioxidant Activity

Objective: Ziziphora clinopodioides is an edible medicinal plant belongs to the Labiatae family that widespread all over Iran. It used as culinary and also in cold and cough treatments in Iran. The aim of present work was to evaluate the effect of different timeframes during the hydrodistillation on essential oil composition, antimicrobial and antioxidant activity. Materials and Methods: The essential oil of Z. clinopodiodes was extracted via hydrodistillation with Clevenger apparatus. The fractions of essential oil were captured at 6 times from the beginning of the distillation: (10, 20, 60, 120, 180 and 240 min). The fractions of essential oil were analyzed by GC/MS and their antibacterial, antifungal and antioxidant activities were studied by Disk - well diffusion and DPPH methods respectively. Results: Six distillation times and whole essential oil were captured during the hydrodistillation. Essential oil yield dropped off significantly during distillation progressed (1.0% for 10 min and 0.025 for 240 min). 1,8 Cineol, Isomenthone, Pulegone, Piperitenone and Citronellic acid were major compounds in fractions and they were affected by distillation times. Pulegone was major compound in all of essential oils. In antioxidant activity assay, whole essential oil was stronger than was stronger than positive control and fractions of essential oil, because of higher levels of Isomenthone, Piperitenone and Citronellic acid. Strongest antimicrobial activity against S. aureus, E. coli and C. albicans was observed from 10 min fraction. Conclusion: Our results indicated that distillation time can create essential oils with specific properties and we can achieve to more efficient essential oil in short times.

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Biological Activities of Carlina Oxide Isolated from the Roots of Carthamus caeruleus

The Natural Products Journal ◽

10.2174/2210315509666190117152740 ◽

2020 ◽

Vol 10 (2) ◽

pp. 145-152 ◽

Cited By ~ 1

Author(s):

Imane Rihab Mami ◽

Rania Belabbes ◽

Mohammed El Amine Dib ◽

Boufeldja Tabti ◽

Jean Costa ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Skin Diseases ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Penicillium Expansum ◽

Natural Food ◽

Antioxidant Power

Background: Carthamus caeruleus belongs to the Asteraceae family. The roots are traditionally used as healing agents. They help to heal burns and treat skin diseases. They are also used against joint inflammation and are very effective against diseases such as irritable bowel syndrome for cancer patients. Objectives: The purpose of this work was i) to study the chemical composition of i) the essential oil and hydrosol extract of Carthamus caeruleus, ii) to isolate the major component of both extracts and iii) to evaluate their antioxidant, antifungal and insecticidal activities. Methods: The essential oil and hydrosol extract obtained from the roots were studied by GC and GC/MS. The antioxidant activities were performed using two different methods i) Radical scavenging activity (DPPH) and ii) the Ferric-Reducing Antioxidant Power (FRAP), using BHT as a positive control. Whereas, the antifungal activity of the essential oil and Carlina oxide was investigated against plant fungi. The fumigation toxicity of C. caeruleus essential oil besides Carlina oxide was evaluated against adults of Bactrocera oleae better known as the olive fly. Results: The essential oil and hydrosol extract were mainly represented by acetylenic compounds such as carline oxide and 13-methoxy carline oxide. Carlina oxide was isolated and identified by 1H and 13C NMR spectroscopic means. The results showed that Carlina oxide presented interesting antioxidant and antifungal properties, while C. caeruleus root essential oil had better insecticidal activity. Furthermore, Carlina oxide has demonstrated promising in vivo antifungal activity to control infection of apples by Penicillium expansum. Conclusion: Carlina oxide can be used as a natural food preservative and alternative to chemical fungicides to protect stored apple against Penicillium expansum.

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Ducrosia ismaelis Asch. essential oil: chemical composition profile and anticancer, antimicrobial and antioxidant potential assessment

Open Chemistry ◽

10.1515/chem-2020-0019 ◽

2020 ◽

Vol 18 (1) ◽

pp. 175-184 ◽

Cited By ~ 1

Author(s):

Ramzi A. Mothana ◽

Fahd A. Nasr ◽

Jamal M. Khaled ◽

Omar M. Noman ◽

Nael Abutaha ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Moderate Activity ◽

Antioxidant Agent ◽

Total Oil ◽

Β Carotene ◽

Mcf 7

AbstractThe essential oil of Ducrosia ismaelis Asch. (Apiaceae) that grows wild in Saudi Arabia was investigated utilizing gas chromatography (GC), and gas chromatography-mass spectrometry. Fifty constituents were characterized, representing 96.1% of the total oil. The D. ismaelis essential oil (DIEO) was distinguished by a high composition of oxygenated monoterpenes (51.6%). Decanal (40.6%), α-pinene (15.1%) and dodecanal (13.7%) were the fundamental components. Additionally, DIEO was evaluated for its cytotoxic, antibacterial, antifungal and antioxidant activities. DIEO revealed a great cytotoxic effectiveness against the tested cancer cell lines with IC50 values between 66.2 and 137.3 μg/mL particularly against MCF-7 cancer cells. Furthermore, the induction of apoptosis against MCF-7 cells has been asserted using staining assay (annexin VFITC and/or propidium iodide (PI) dyes) and flow cytometry technique. The DIEO possessed a strong antimicrobial activity against Gram-positive bacterial and fungal strains with MIC-values between 0.07 and 0.31 mg/ml. The values of MBC or MFC were almost once higher than those of MIC’s. Moreover, the β-carotene-bleaching and DPPH free radical-scavenging tests showed that DIEO had a moderate activity (68%) as an antioxidant agent in decolouring of the β-carotene at 1.0 mg/mL and a moderate radical scavenging for DPPH (66 and 72%) at 0.50 and 1.0 mg/mL.

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Antimicrobial Essential Oil Combinations to Combat Foot Odour

Planta Medica ◽

10.1055/a-0592-8022 ◽

2018 ◽

Vol 84 (09/10) ◽

pp. 662-673 ◽

Cited By ~ 4

Author(s):

Ané Orchard ◽

Alvaro Viljoen ◽

Sandy van Vuuren

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Efficacy ◽

Juniperus Virginiana ◽

Broth Microdilution ◽

Synergistic Interactions ◽

Microdilution Method ◽

Broth Microdilution Method

AbstractFoot odour (bromodosis) is an embarrassing and perplexing condition mostly caused by bacteria of the Brevibacterium species. Essential oils are a credible option as an affordable treatment of odour and contribute towards antimicrobial efficacy. Therefore, this study sets out to investigate the antimicrobial activity of essential oil combinations against odour-causing bacteria. The broth microdilution method was used to investigate the antimicrobial activity of 119 essential oil combinations, and the fractional inhibitory index was calculated to determine the interactive profile. Combinations that resulted in synergy in 1 : 1 ratios were further evaluated in different concentrations, and isobolograms were plotted to determine the influence of the ratio on overall activity. Numerous combinations could be identified as having synergistic interactions against the Brevibacterium spp. and no antagonism was observed. The combination of Juniperus virginiana (juniper) and Styrax benzoin (benzoin) demonstrated synergy against all three Brevibacterium spp. tested and J. virginiana was the essential oil responsible for the majority of the synergistic interactions. The results reported here confirm the promising potential of the majority of these oils and selected combinations in treating and controlling bromodosis.

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Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

Journal of Chemistry ◽

10.1155/2015/804658 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 35

Author(s):

Kamel Msaada ◽

Nidhal Salem ◽

Olfa Bachrouch ◽

Slim Bousselmi ◽

Sonia Tammar ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Essential Oils ◽

Methanolic Extract ◽

Biological Activities ◽

Reducing Power ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Oil Composition ◽

Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

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