Effect of Sucrose Containing Iron (II) on Dental Biofilm and Enamel Demineralization in situ

2005 ◽  
Vol 39 (2) ◽  
pp. 123-129 ◽  
Author(s):  
G.D. Pecharki ◽  
J.A. Cury ◽  
A.F. Paes Leme ◽  
C.P.M. Tabchoury ◽  
A.A. Del Bel Cury ◽  
...  
Keyword(s):  
Dental Biofilm ◽  
Enamel Demineralization

scholarly journals In situ Assessment of Effects of the Bromide- and Fluoride-incorporating Adhesive Systems on Biofilm and Secondary Caries

2014 ◽  
Vol 15 (2) ◽  
pp. 142-148 ◽  
Author(s):  
Suyane Maria Luna Cruz de Vasconcelos ◽  
Mary Anne Sampaio de Melo ◽  
Joao Paulo Marques Saraiva Wenceslau ◽  
Iriana Carla Junqueira Zanin ◽  
Haroldo Cesar Pinheiro Beltrao ◽  
...  
Keyword(s):  
Mutans Streptococci ◽  
Adhesive Systems ◽  
Double Blind ◽  
Secondary Caries ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Significant Difference ◽  
In Situ Assessment ◽  
Self Etch

ABSTRACT Aim This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization. Materials and methods During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SETM (self-etch, fluoride/MDPB free adhesive, AB) and Clearfil Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S (ƒ¢S) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 ƒÊm from the restoration margin. Data were analyzed by ANOVA. Results No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm. Conclusion All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research. How to cite this article de Vasconcelos SMLC, de Melo MAS, Wenceslau JPMS, Zanin ICJ, Beltrao HCP, Fernandes CAO, de Almeida PC, Rodrigues LKA. In situ Assessment of Effects of the Bromide- and Fluoride-Incorporating Adhesive Systems on Biofilm and Secondary Caries. J Contemp Dent Pract 2014;15(2):142-148.

scholarly journals Effect of starch on the cariogenic potential of sucrose

2005 ◽  
Vol 94 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Cecilia C. C. Ribeiro ◽  
Cínthia P. M. Tabchoury ◽  
Altair A. Del Bel Cury ◽  
Livia M. A. Tenuta ◽  
Pedro L. Rosalen ◽  
...  
Keyword(s):  
Animal Studies ◽  
Amylase Activity ◽  
Negative Control ◽  
Cross Sectional ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Two Phases ◽  
Mineral Loss

Sincein vitroand animal studies suggest that the combination of starch with sucrose may be more cariogenic than sucrose alone, the study assessedin situthe effects of this association appliedin vitroon the acidogenicity, biochemical and microbiological composition of dental biofilm, as well as on enamel demineralization. During two phases of 14 d each, fifteen volunteers wore palatal appliances containing blocks of human deciduous enamel, which were extra-orally submitted to four groups of treatments: water (negative control, T1); 2 % starch (T2); 10 % sucrose (T3); and 2 % starch+10 % sucrose (T4). The solutions were dripped onto the blocks eight times per day. The biofilm formed on the blocks was analysed with regard to amylase activity, acidogenicity, and biochemical and microbiological composition. Demineralization was determined on enamel by cross-sectional microhardness. The greatest mineral loss was observed for the association starch+sucrose (P<0·05). Also, this association resulted in the highest lactobacillus count in the biofilm formed (P<0·05). In conclusion, the findings suggest that a small amount of added starch increases the cariogenic potential of sucrose.

Effect of Sucrose Concentration on Dental Biofilm Formed in situ and on Enamel Demineralization

2005 ◽  
Vol 40 (1) ◽  
pp. 28-32 ◽  
Author(s):  
C.P. Aires ◽  
C.P.M. Tabchoury ◽  
A.A. Del Bel Cury ◽  
H. Koo ◽  
J.A. Cury
Keyword(s):  
Sucrose Concentration ◽  
Dental Biofilm ◽  
Enamel Demineralization

Effectiveness of a Toothpaste with Low Fluoride Content Combined with Trimetaphosphate on Dental Biofilm and Enamel Demineralization in situ

2015 ◽  
Vol 49 (4) ◽  
pp. 394-400 ◽  
Author(s):  
Eliana M. Takeshita ◽  
Marcelle Danelon ◽  
Luciene P. Castro ◽  
Kikue T. Sassaki ◽  
Alberto C.B. Delbem
Keyword(s):  
Sucrose Solution ◽  
Blind Study ◽  
Double Blind Study ◽  
Oral Appliances ◽  
Double Blind ◽  
Sodium Trimetaphosphate ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Mineral Loss

Objective: The aim of the present study was to evaluate in situ whether a toothpaste with low fluoride associated with sodium trimetaphosphate (TMP) would provide similar effect to that of a 1,100 ppm F toothpaste. Design: This crossover double-blind study consisted of 4 phases (14 days each), during which 10 volunteers wore oral appliances containing 4 enamel bovine blocks. The cariogenic challenge was performed by the application of a 20% sucrose solution (6×/day). The toothpaste treatments (2×/day) were: placebo, 500 ppm F, 500 ppm F plus 1% TMP, and 1,100 ppm F. At the end, enamel mineral loss and biofilm composition were analyzed. Results: The toothpaste with 500 ppm F plus 1% TMP showed the lowest mineral loss (p < 0.05). Regarding the fluoride and calcium concentrations in the enamel and in the biofilm, there were no significant differences between 500 ppm F plus 1% TMP, and 1,100 ppm F toothpastes (p > 0.569), but they were significantly different when compared to toothpaste with 500 ppm F (p < 0.050). Conclusion: The addition of 1% TMP to a low-fluoride toothpaste reduces enamel demineralization in situ similar to a 1,100 ppm F toothpaste.

In situ evaluation of 200 ppm fluoride toothpaste content trimetaphosphate, xylitol and erythritol on enamel demineralization and dental biofilm

2021 ◽  
pp. 103724
Author(s):  
Rômulo Andrade Marcato ◽  
Cássia Cilene Dezan Garbelini ◽  
Marcelle Danelon ◽  
Juliano Pelim Pessan ◽  
Nayara Gonçalves Emerenciano ◽  
...  
Keyword(s):  
Fluoride Toothpaste ◽  
Dental Biofilm ◽  
Enamel Demineralization

scholarly journals Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure

2007 ◽  
Vol 18 (3) ◽  
pp. 185-191 ◽  
Author(s):  
Rodrigo Alex Arthur ◽  
Cínthia Pereira Machado Tabchoury ◽  
Renata de Oliveira Mattos-Graner ◽  
Altair A. Del Bel Cury ◽  
Adriana Franco Paes Leme ◽  
...  
Keyword(s):  
Genotypic Diversity ◽  
Negative Control ◽  
Stress Exposure ◽  
Chain Reaction ◽  
Dental Biofilm ◽  
Polymerase Chain ◽  
Fermentable Carbohydrates ◽  
Almost All ◽  
Frequent Exposure

In situ dental biofilm composition under sugar exposure is well known, but sugar effect on the genotypic diversity of S. mutans in dental biofilm has not been explored. This study evaluated S. mutans genotypic diversity in dental biofilm formed in situ under frequent exposure to sucrose and its monosaccharide constituents (glucose and fructose). Saliva of 7 volunteers was collected for isolation of S. mutans and the same volunteers wore intraoral palatal appliances, containing enamel slabs, which were submitted to the following treatments: distilled and deionized water (negative control), 10% glucose + 10% fructose (fermentable carbohydrates) solution or 20% sucrose (fermentable and EPS inductor) solution, 8x/day. After 3, 7 and 14 days, the biofilms were colleted and S. mutans colonies were isolated. Arbitrarily primed polymerase chain reaction (AP-PCR) of S. mutans showed that salivary genotypes were also detected in almost all biofilm samples, independently of the treatment, and seemed to reflect those genotypes present at higher proportion in biofilms. In addition to the salivary genotypes, others were found in biofilms but in lower proportions and were distinct among treatment. The data suggest that the in situ model seems to be useful to evaluate genotypic diversity of S. mutans, but, under the tested conditions, it was not possible to clearly show that specific genotypes were selected in the biofilm due to the stress induced by sucrose metabolism or simple fermentation of its monosaccharides.

Enamel Demineralization in situ with Various Frequencies of Carbohydrate Consumption with and without Fluoride Toothpaste

2001 ◽  
Vol 80 (8) ◽  
pp. 1721-1724 ◽  
Author(s):  
M.S. Duggal ◽  
K.J. Toumba ◽  
B.T. Amaechi ◽  
M.B. Kowash ◽  
S.M. Higham
Keyword(s):  
Carbohydrate Consumption ◽  
Fluoride Toothpaste ◽  
Enamel Demineralization

scholarly journals Effects of Experimental Agents Containing Tannic Acid or Chitosan on the Bacterial Biofilm Formation in Situ

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1315
Author(s):  
Anton Schestakow ◽  
Matthias Hannig
Keyword(s):  
Hydrochloric Acid ◽  
Tannic Acid ◽  
Biofilm Formation ◽  
Antibacterial Properties ◽  
Positive Control ◽  
Bacterial Biofilm ◽  
Dental Biofilm ◽  
Acid Ph ◽  
Bovine Enamel

Chitosan and tannic acid are known for their antibacterial properties. In the present in-situ study, their antibacterial and anti-adherent effects on biofilm formation on enamel were investigated. Six subjects carried upper jaw splints with bovine enamel specimens, allowing in-situ biofilm formation. During the two-day trial, subjects rinsed with experimental solutions that contained either chitosan, tannic acid (pH = 2.5), tannic acid (pH = 7) or hydrochloric acid. Water served as the negative and chlorhexidine as the positive control. Rinsing occurred four or five times following two different rinsing protocols to investigate both the immediate and long-lasting effects. After 48 h of intraoral exposure, the dental plaque was stained with LIVE/DEAD® BacLight, and fluorescence micrographs were evaluated by using the software ImageJ. The results were verified by scanning electron microscopy. Rinsing with chitosan resulted in little immediate antibacterial and anti-adherent effects but failed to show any long-lasting effect, while rinsing with tannic acid resulted in strong immediate and long-lasting effects. Except for a slightly lower antibacterial effect, the neutral solution of tannic acid was as good as the acidic solution. Hydrochloric acid showed neither an antibacterial nor an anti-adherent effect on dental biofilm formation. Experimental solutions containing tannic acid are promising anti-biofilm agents, irrespective of the pH values of the solutions. Chitosan, on the other hand, was not able to prevent biofilm formation.

Effect of Dentifrice Containing Fluoride and/or Baking Soda on Enamel Demineralization/ Remineralization: An in situ Study

2001 ◽  
Vol 35 (2) ◽  
pp. 106-110 ◽  
Author(s):  
J.A. Cury ◽  
L.N. Hashizume ◽  
A.A. Del Bel Cury ◽  
C.P.M. Tabchoury
Keyword(s):  
In Situ Study ◽  
Enamel Demineralization
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